Piezo1-targeted aerosol inhalation nanoparticles for acute lung injury

Inhaled drug-containing nanocarriers have been well applied as an important strategy in pulmonary dis-eases.Acute lung injury(ALI)is characterized by abnormal lung tension and complex pathogenesis,with high mortality because of the limitations of targeted intervention.Accordingly,in the present study,we first identified that the mechanosensitive ion channel Piezo1 participated in the ALI-associated processes induced by lipopolysaccharide(LPS)in vitro and in vivo.Then,chitosan-stabilized bovine serum albu-min nanoparticles(NCs)emulsified with the Piezo1 inhibitor GsMTx4(NC-GsMTx4)were generated and exhibited excellent biocompatibility and biological function.Through aerosol inhalation,NC-GsMTx4 im-proved lung injury and inhibited cell apoptosis in LPS-stressed ALI mice and alleviated pulmonary fibrosis during the later stage of ALI.Mechanistically,GsMTx4 could regulate inflammation and apoptosis in lung epithelial cells via NF-κB and ERK1/2 signaling.In summary,our findings provide new insights into the pathological mechanisms of Piezo1 in ALI progression,and nebulized inhalation of NC-GsMTx4 offers a prospective platform for targeting Piezo1 to treat ALI efficiently and conveniently.

Photodegradation of benzene by TiO_2 nanoparticles prepared by flame CVD process

Photodegradation of benzene at ppb levels by mixed-phase TiO2 nanoparticles, synthesized by the oxidation of TiCl4 in propane/air turbulent flame chemical vapor deposition （CVD） process, is investigated experimentally by using a tubular photoreactor with thin TiO2 films coated on the reactor wall by sedimentation. Effects of inlet benzene concentration from 10 to 300μg/m3, rutile mass fraction from about 20 to 50% and photoluminescence （PL） intensity of TiO2 nanoparticles on degradation degree are examined under the conditions of 70% relative humidity, 38 μg/cm2 catalyst loading, 24mW/cm2 UV irradiation of 254 nm and 5.7 s residence time in the reactor. Based on experimental results, separation of photoinduced electron （e-） and hole （h＋） pairs by rutile phase is discussed as photo-induced electron （e-） in anatase phase will migrate to rutile surface due to that the potential of conductive band of rutile is lower than that of anatase, leading to more holes ready on anatase surface for oxidation reactions.

Cisplatin-loaded Poly(L-glutamic acid)-g-Methoxy Poly(ethylene glycol) Nanoparticles as a Potential Chemotherapeutic Agent against Osteosarcoma

Herein, cisplatin-loaded poly（L-glutamic acid）-g-methoxy poly（ethylene glycol） nanoparticles were evaluated as a potential chemotherapeutic agent against osteosarcoma by using alone or with an i RGD（internalizing RGD, CRGDKDPDC）. The release rate of platinum from the cisplatin-loaded nanoparticles CDDP/PLG160-g-m PEG2K（CDDP-NPs） accelerated with the increase of the acidity of the environment. In vitro test demonstrated that CDDP-NPs could inhibit the proliferation of MNNG/Hos osteosarcoma cells with IC50（72 h） of 12.2 μg·mL^-1. In vivo test for MNNG/Hos osteosarcoma tumor bearing mice exhibited that CDDP-NPs had comparable or slightly higher efficacy but significantly lower side effects in comparison with free CDDP. The coadministration of i RGD could further enhance the anticancer efficacy of CDDP-NPs against MNNG/Hos osteosarcoma without bringing obvious side effects. Therefore, CDDP-NPs using alone or with iRGD have great potential for the treatment of osteosarcoma.