The composition of materials in a micro-/nano-devices plays a key role in determining their mechanical,physical,and chemical properties.Especially,for devices with a compositional change on nanoscale which can often b...The composition of materials in a micro-/nano-devices plays a key role in determining their mechanical,physical,and chemical properties.Especially,for devices with a compositional change on nanoscale which can often be achieved by point-by-point direct writing technology using a focused ion beam(FIB),electron beam(EB),or laser beam(LB),but so far,nanoscale composition analysis of a large-area micro/nano structures with a variation composition remains a big challenge in cost,simpleness,and flexibility.Here we present a feasible route to realize large-area composition analysis with nanoscale spatial resolution by using Raman spectroscopy.We experimentally verified the capability of this method by analyzing a complex Sn-SnOx system of a microscale grayscale mask with nanoscale spatial resolution of composition.Further analyses using Auger electron spectroscopy,transmission electron microscopy,and atomic force microscopy indicated the effectiveness and practicality of our method.This work opens up a way to analyze the composition of a large-area complex system at a nanoscale spatial resolution,and the method can be extended to many other material systems.展开更多
Fluorescence nanoscopy provides imaging techniques that overcome the diffraction-limited resolution barrier in light microscopy,thereby opening up a new area of research in biomedical imaging in fields such as neurosc...Fluorescence nanoscopy provides imaging techniques that overcome the diffraction-limited resolution barrier in light microscopy,thereby opening up a new area of research in biomedical imaging in fields such as neuroscience.Here,we review the foremost fluorescence nanoscopy techniques,including descriptions of their applications in elucidating protein architectures and mobility,the real-time determination of synaptic parameters involved in neural processes,three-dimensional imaging,and the tracking of nanoscale neural activity.We conclude by discussing the prospects of fluorescence nanoscopy,with a particular focus on its deployment in combination with related techniques(e.g.,machine learning)in neuroscience.展开更多
基金This work was supported by the National Natural Science Foundation of China(Nos.51971070,and 10974037)the national Key Research and Development Program of China(No.2016YFA0200403)+4 种基金the CAS Strategy Pilot Program(No.XDA 09020300)Eu-FP7 Project(No 247644)Guangdong Provincial Key Laboratory Program(No.2021B1212040001)the Department of Science and Technology of Guangdong ProvinceThe work performed at SZTU was supported by NSFC(No.61805156)。
文摘The composition of materials in a micro-/nano-devices plays a key role in determining their mechanical,physical,and chemical properties.Especially,for devices with a compositional change on nanoscale which can often be achieved by point-by-point direct writing technology using a focused ion beam(FIB),electron beam(EB),or laser beam(LB),but so far,nanoscale composition analysis of a large-area micro/nano structures with a variation composition remains a big challenge in cost,simpleness,and flexibility.Here we present a feasible route to realize large-area composition analysis with nanoscale spatial resolution by using Raman spectroscopy.We experimentally verified the capability of this method by analyzing a complex Sn-SnOx system of a microscale grayscale mask with nanoscale spatial resolution of composition.Further analyses using Auger electron spectroscopy,transmission electron microscopy,and atomic force microscopy indicated the effectiveness and practicality of our method.This work opens up a way to analyze the composition of a large-area complex system at a nanoscale spatial resolution,and the method can be extended to many other material systems.
基金the Zhangjiang National Innovation Demonstration Zone(ZJ2019-ZD-005)the National Natural Science Foundation of China(11874267)supported by a fellowship of the China Postdoctoral Science Foundation(2020M671169)。
文摘Fluorescence nanoscopy provides imaging techniques that overcome the diffraction-limited resolution barrier in light microscopy,thereby opening up a new area of research in biomedical imaging in fields such as neuroscience.Here,we review the foremost fluorescence nanoscopy techniques,including descriptions of their applications in elucidating protein architectures and mobility,the real-time determination of synaptic parameters involved in neural processes,three-dimensional imaging,and the tracking of nanoscale neural activity.We conclude by discussing the prospects of fluorescence nanoscopy,with a particular focus on its deployment in combination with related techniques(e.g.,machine learning)in neuroscience.
