Surface-enhanced Raman scattering(SERS)spectroscopy is presented as a sensitive and spe-cific molecular tool for clinical diagnosis and prognosis monitoring of various diseases including cancer.In order for clinical a...Surface-enhanced Raman scattering(SERS)spectroscopy is presented as a sensitive and spe-cific molecular tool for clinical diagnosis and prognosis monitoring of various diseases including cancer.In order for clinical application of SERS technique,an ideal method of bulk synthesis of SERS nanoparticles is necessary to obtain sensitive,stable and highly reproducible Raman signals.In this contribution,we determined the ideal conditions for bulk synthesis of Raman reporter(Ra)molecules embedded silver-gold core-shell nanoparticles(Au@Ra@AgNPs)using hydroquinone as reducing agent of silver nitrate.By using UV-Vis spectroscopy,Raman spectroscopy and transmission electron microscopy(TEM),we found that a 2∶1 ratio of silver nitrate to hydroquinone is ideal for a uniform silver coating with a strong and stable Raman signal.Through stability testing of the optimized Au@Ra@AgNPs over a two-week period,these SERS nanotags were found to be stable with minimal signal change occurred.The sta-bility of antibody linked SERS nanotags is also crucial for cancer and disease diagnosis,thus,we further conjugated the as-prepared SERS nanotags with anti-EpCAM antibody,in which the stability of bioconjugated SERS nanotags was tested over eight days.Both UV-Vis and SERS spectroscopy showed stable absorption and Raman signals on the anti-EpCAM conju-gated SERS nanotags,indicating the great potential of the synthesized SERS nanotags for future applications which require large,reproducible and uniform quantities in order for cancer biomarker diagnosis and monitoring.展开更多
Owing to the unique advantages of surface enhanced Raman scattering(SERS)in high sensitivity,specificity,multiplexing capability and photostability,it has been widely used in many applications,among which SERS biosens...Owing to the unique advantages of surface enhanced Raman scattering(SERS)in high sensitivity,specificity,multiplexing capability and photostability,it has been widely used in many applications,among which SERS biosensing and bioimaging are the focus in recent years.The successful applications of SERS for non-invasive biomarker detection and bioimaging under in vitro,in vivo and ex vivo conditions,offer significant clinical information to improve diagnostic and prognostic outcomes.This review provides recent developments and applications of SERS,in particular SERS nanotags in biosensing and bioimaging,describing case studies in which different types of biomarkers have been investigated,as well as outlining future challenges that need to be addressed before SERS sees both pathological and clinical use.展开更多
基金This work was supported by the Australian Research Council(ARC)through its Centre of Excellence for Nanoscale BioPhotonics(CE140100003)ARC Discovery Projects(DP200102004).
文摘Surface-enhanced Raman scattering(SERS)spectroscopy is presented as a sensitive and spe-cific molecular tool for clinical diagnosis and prognosis monitoring of various diseases including cancer.In order for clinical application of SERS technique,an ideal method of bulk synthesis of SERS nanoparticles is necessary to obtain sensitive,stable and highly reproducible Raman signals.In this contribution,we determined the ideal conditions for bulk synthesis of Raman reporter(Ra)molecules embedded silver-gold core-shell nanoparticles(Au@Ra@AgNPs)using hydroquinone as reducing agent of silver nitrate.By using UV-Vis spectroscopy,Raman spectroscopy and transmission electron microscopy(TEM),we found that a 2∶1 ratio of silver nitrate to hydroquinone is ideal for a uniform silver coating with a strong and stable Raman signal.Through stability testing of the optimized Au@Ra@AgNPs over a two-week period,these SERS nanotags were found to be stable with minimal signal change occurred.The sta-bility of antibody linked SERS nanotags is also crucial for cancer and disease diagnosis,thus,we further conjugated the as-prepared SERS nanotags with anti-EpCAM antibody,in which the stability of bioconjugated SERS nanotags was tested over eight days.Both UV-Vis and SERS spectroscopy showed stable absorption and Raman signals on the anti-EpCAM conju-gated SERS nanotags,indicating the great potential of the synthesized SERS nanotags for future applications which require large,reproducible and uniform quantities in order for cancer biomarker diagnosis and monitoring.
基金the Australian Research Council(ARC)Discovery Early Career Research Award(DECRA-DE 140101056)to Y.W.
文摘Owing to the unique advantages of surface enhanced Raman scattering(SERS)in high sensitivity,specificity,multiplexing capability and photostability,it has been widely used in many applications,among which SERS biosensing and bioimaging are the focus in recent years.The successful applications of SERS for non-invasive biomarker detection and bioimaging under in vitro,in vivo and ex vivo conditions,offer significant clinical information to improve diagnostic and prognostic outcomes.This review provides recent developments and applications of SERS,in particular SERS nanotags in biosensing and bioimaging,describing case studies in which different types of biomarkers have been investigated,as well as outlining future challenges that need to be addressed before SERS sees both pathological and clinical use.
