Oil content is a crucial indicator for evaluating the quality of peanuts.A rapid and non-destructive method to determine oil content of individual peanut seed can provide robust technical support for breeding high-oil...Oil content is a crucial indicator for evaluating the quality of peanuts.A rapid and non-destructive method to determine oil content of individual peanut seed can provide robust technical support for breeding high-oil-content peanut varieties.In this study,we established a rapid determination method using near-infrared hyperspectral imaging and chemometrics to assess the oil content of single peanut seed.After selecting key wavelengths through competitive adaptive reweighted sampling(CARS),uninformative variable elimination(UVE),and random frog(RF),we constructed an oil content calibration model based on partial least squares regression for single peanut seed.Validation results demonstrated that the correlation coefficient was 0.8393 with a root mean square error of 1.7771 in the calibration set,while it was 0.7915 with a root mean square error of 2.2943 in the independent prediction set.Most samples exhibited relative errors below 5%,confirming the reliability of this model in predicting oil content of single peanut seed.展开更多
To ensure the quality and safety of pure milk,detection method of typical preservative-potassium sorbate in milk was researched in this paper.Hyperspectral imaging technology was applied to realize rapid detection.Inf...To ensure the quality and safety of pure milk,detection method of typical preservative-potassium sorbate in milk was researched in this paper.Hyperspectral imaging technology was applied to realize rapid detection.Influence factors for hyperspectral data collection for milk samples were firstly researched,including height of sample,bottom color and sample filled up container or not.Pretreatment methods and variable selection algorithms were applied into original spectral data.Rapid detection models were built based on support vector machine method(SVM).Finally,standard normalized variable(SNV)-competitive adaptive reweighted sampling(CARS)and SVM model was chosen in this paper.The accuracies of calibration set and testing set were 0.97 and 0.97,respectively.Kappa coefficient of the model was 0.93.It could be seen that hyperspectral imaging technology could be used to detect for potassium sorbate in milk.Meanwhile,it also provided methodological supports for the rapid detection of other preservatives in milk.展开更多
We observed atherosclerotic plaque phantoms using a novel near-infrared (NIR) hyperspectral imaging (HSI) technique. Data were obtained through saline or blood layers to simulate an angioscopic environment for the pha...We observed atherosclerotic plaque phantoms using a novel near-infrared (NIR) hyperspectral imaging (HSI) technique. Data were obtained through saline or blood layers to simulate an angioscopic environment for the phantom. For the study, we developed a NIR-HSI system with an NIR supercontinuum light source and mercury-cadmium-telluride camera. Apparent spectral absorbance was obtained at wavelengths of 1150 - 2400 nm. Hyperspectral images of lipid were constructed using a spectral angle mapper algorithm. Bovine fat covered with saline or blood was observed using hyperspectral images at a wavelength around 1200 nm. Our results show that NIR-HSI is a promising angioscopic technique with the potential to identify lipid-rich plaques without clamping and saline injection.展开更多
Tea plant stresses threaten the quality of tea seriously.The technology corresponding to the fast detection and differentiation of stresses is of great significance for plant protection in tea plantation.In recent yea...Tea plant stresses threaten the quality of tea seriously.The technology corresponding to the fast detection and differentiation of stresses is of great significance for plant protection in tea plantation.In recent years,hyperspectral imaging technology has shown great potential in detecting and differentiating plant diseases,pests and some other stresses at the leaf level.However,the lack of studies at canopy level hampers the detection of tea plant stresses at a larger scale.In this study,based on the canopy-level hyperspectral imaging data,the methods for identifying and differentiating the three commonly occurred tea stresses(i.e.,the tea leafhopper,anthrax and sun burn)were studied.To account for the complexity of the canopy scenario,a stepwise detecting strategy was proposed that includes the process of background removal,identification of damaged areas and discrimination of stresses.Firstly,combining the successive projection algorithm(SPA)spectral analysis and K-means cluster analysis,the background and overexposed non-plant regions were removed from the image.Then,a rigorous sensitivity analysis and optimization were performed on various forms of spectral features,which yielded optimal features for detecting damaged areas(i.e.,YSV,Area,GI,CARI and NBNDVI)and optimal features for stresses discrimination(i.e.,MCARI,CI,LCI,RARS,TCI and VOG).Based on this information,the models for identifying damaged areas and those models for discriminating different stresses were established using K-nearest neighbor(KNN),Random Forest(RF)and Fisher discriminant analysis.The identification model achieved an accuracy over 95%,and the discrimination model achieved an accuracy over 93%for all stresses.The results suggested the feasibility of stress detection and differentiation using canopy-level hyperspectral imaging techniques,and indicated the potential for its extension over large areas.展开更多
Objective:We applied hyperspectral imaging(HSI)system to distinguish early caries from soundand pigmented areas.It will provide a theoretical basis and technical support,for research anddevelopment of an instrument th...Objective:We applied hyperspectral imaging(HSI)system to distinguish early caries from soundand pigmented areas.It will provide a theoretical basis and technical support,for research anddevelopment of an instrument that could be used for screening and detection of early dentalcaries.Methods:Eighteen extracted human teeth(molars and premolars),with varying degrees ofnatural pathology and no degree of decay involving dentin were obtained.HSI system with awavelength range from 400 to 1000nm was used to obtain images of all 18 teeth containingsound,carious and pigmented areas.We compared the spectra of the wavebands at both 500 nmand 780 nm from the different tooth states,and the reflectance diference bet ween sound versuscarious lesions and sound versus pigmented areas,respectively.Results:There was a slight diference in refectance bet ween carious areas and pigmented areas at500 nm.A substantial difference was additionally noted in refectance bet ween carious areas andpigmented areas at 780 nm.Conclusion:The results have shown that the interference of tooth surface pigment can be elim-inated in the near-infrared(NIR)waveband,and the caries can be effectively identifed from the pigmented areas.Thus,it could be used to detect carious areas of teeth in place of the traditionalvisual inspection method or white light endoscopy.Clinical significance:The NIR difused light signal enables the identification of early caries frompigment and other interference,providing a reasonable detection tool for early detection andearly treatment of teeth diseases.展开更多
As unsafe components in herbal medicine(HM),saccharides can affect not only the drug appearance and stabilization,but also the drug efficacy and safety.The present study focuses on the in-line monitoring of batch alco...As unsafe components in herbal medicine(HM),saccharides can affect not only the drug appearance and stabilization,but also the drug efficacy and safety.The present study focuses on the in-line monitoring of batch alcohol precipitation processes for saccharide removal using nearinfrared(NIR)spectroscopy.NIR spectra in the 4000–10,000-cm^(-1)wavelength range are acquired in situ using a transflectance probe.These directly acquired spectra allow characterization of the dynamic variation tendency of saccharides during alcohol precipitation.Calibration models based on partial least squares(PLS)regression have been developed for the three saccharide impurities,namely glucose,fructose,and sucrose.Model errors are estimated as the root-meansquare errors of cross-validation(RMSECVs)of internal validation and root-mean-square errors of prediction(RMSEPs)of external validation.The RMSECV values of glucose,fructose,and sucrose were 1.150,1.535,and 3.067 mg·mL^(-1),and the RMSEP values were 0.711,1.547,and 3.740 mg·mL^(-1),respectively.The correlation coeffcients(r)between the NIR predictive and the reference measurement values were all above 0.94.Furthermore,NIR predictions based on the constructed models improved our understanding of sugar removal and helped develop a control strategy for alcohol precipitation.The results demonstrate that,as an alternative process analytical technology(PAT)tool for monitoring batch alcohol precipitation processes,NIR spectroscopy is advantageous for both efficient determination of quality characteristics(fast,in situ,and requiring no toxic reagents)and process stability,and evaluating the repeatability.展开更多
Hyperspectral imaging is gaining a significant role in agricultural remote sensing applications.Its data unit is the hyperspectral cube which holds spatial information in two dimensions while spectral band information...Hyperspectral imaging is gaining a significant role in agricultural remote sensing applications.Its data unit is the hyperspectral cube which holds spatial information in two dimensions while spectral band information of each pixel in the third dimension.The classification accuracy of hyperspectral images(HSI)increases significantly by employing both spatial and spectral features.For this work,the data was acquired using an airborne hyperspectral imager system which collected HSI in the visible and near-infrared(VNIR)range of 400 to 1000 nm wavelength within 180 spectral bands.The dataset is collected for nine different crops on agricultural land with a spectral resolution of 3.3 nm wavelength for each pixel.The data was cleaned from geometric distortions and stored with the class labels and annotations of global localization using the inertial navigation system.In this study,a unique pixel-based approach was designed to improve the crops'classification accuracy by using the edge-preserving features(EPF)and principal component analysis(PCA)in conjunction.The preliminary processing generated the high-dimensional EPF stack by applying the edge-preserving filters on acquired HSI.In the second step,this high dimensional stack was treated with the PCA for dimensionality reduction without losing significant spectral information.The resultant feature space(PCA-EPF)demonstrated enhanced class separability for improved crop classification with reduced dimensionality and computational cost.The support vector machines classifier was employed for multiclass classification of target crops using PCA-EPF.The classification performance evaluation was measured in terms of individual class accuracy,overall accuracy,average accuracy,and Cohen kappa factor.The proposed scheme achieved greater than 90%results for all the performance evaluation metrics.The PCA-EPF proved to be an effective attribute for crop classification using hyperspectral imaging in the VNIR range.The proposed scheme is well-suited for practical applications of crops and landfill estimations using agricultural remote sensing methods.展开更多
Efficient and accurate acquisition of the rice grain protein content(GPC)is important for selecting high-quality rice varieties,and remote sensing technology is an attractive potential method for this task.However,the...Efficient and accurate acquisition of the rice grain protein content(GPC)is important for selecting high-quality rice varieties,and remote sensing technology is an attractive potential method for this task.However,the majority of multispectral sensors are poor predictors of GPC due to their broad spectral bands.Hyperspectral technology provides a new analytical technology for bridging the gap between phenomics and genomics.However,the small size of typical datasets is a constraint for model construction for estimating GPC,limiting their accuracy and reducing their ability to generalize to a wide range of varieties.In this study,we used hyperspectral data of rice grains from 515 japonica varieties and deep convolution generative adversarial networks(DCGANs)to generate simulated data to improve the model accuracy.Features sensitive to GPC were extracted after applying a continuous wavelet transform(CWT),and the estimated GPC model was constructed by partial least squares regression(PLSR).Finally,a genome-wide association study(GWAS)was applied to the measured and generated datasets to detect GPC loci.The results demonstrated that the simulated GPC values generated after 8,000 epochs were closest to the measured values.The wavelet feature(WF_(1743,2)),obtained from the data with the addition of 200 simulated samples,exhibited the highest GPC estimation accuracy(R^(2)=0.58 and RRMSE=6.70%).The GWAS analysis showed that the estimated values based on the simulated data detected the same loci as the measured values,including the OsmtSSB1L gene related to grain storage protein.This study provides a new technique for the efficient genetic study of phenotypic traits in rice based on hyperspectral technology.展开更多
OBJECTIVE:To evaluate the quality of Moyao(Myrrh)in the identification of the geographical origin and processing of the products.METHODS:Raw Moyao(Myrrh)and two kinds of Moyao(Myrrh)processed with vinegar from three c...OBJECTIVE:To evaluate the quality of Moyao(Myrrh)in the identification of the geographical origin and processing of the products.METHODS:Raw Moyao(Myrrh)and two kinds of Moyao(Myrrh)processed with vinegar from three countries were identified using near-infrared(NIR)spectroscopy combined with chemometric techniques.Principal component analysis(PCA)was used to reduce the dimensionality of the data and visualize the clustering of samples from different categories.A classical chemometric algorithm(PLS-DA)and two machine learning algorithms[K-nearest neighbor(KNN)and support vector machine]were used to conduct a classification analysis of the near-infrared spectra of the Moyao(Myrrh)samples,and their discriminative performance was evaluated.RESULTS:Based on the accuracy,precision,recall rate,and F1 value in each model,the results showed that the classical chemometric algorithm and the machine learning algorithm obtained positive results.In all of the chemometric analyses,the NIR spectrum of Moyao(Myrrh)preprocessed by standard normal variation or Multivariate scattering correction combined with KNN achieved the highest accuracy in identifying the geographical origins,and the accuracy of identifying the processing technology established by the KNN method after first-order derivative pretreatment was the best.The best accuracy of geographical origin discrimination and processing technology discrimination were 0.9853 and 0.9706 respectively.CONCLUSIONS:NIR spectroscopy combined with chemometric technology can be an important tool for tracking the origin and processing technology of Moyao(Myrrh)and can also provide a reference for evaluations of its quality and the clinical use.展开更多
This study was carried out to investigate the feasibility of using visible and near infrared hyperspectral imaging for the variety classification of mung beans.Raw hyperspectral images of mung beans were acquired in t...This study was carried out to investigate the feasibility of using visible and near infrared hyperspectral imaging for the variety classification of mung beans.Raw hyperspectral images of mung beans were acquired in the wavelengths of 380-1023 nm,and all images were calibrated by the white and dark reference images.The spectral reflectance values were extracted from the region of interest(ROI)of each calibrated hyperspectral image,and then they were treated as the independent variables.The dependent variables of four varieties of mung beans were set as 1,2,3 and 4,respectively.The extreme learning machine(ELM)model was established using full spectral wavelengths for classification.Modified gram-schmidt(MGS)method was used to identify effective wavelengths.Based on the selected wavelengths,the ELM and linear discriminant analysis(LDA)models were built.All models performed excellently with the correct classification rates(CCRs)covering 99.17%-99.58% in the training sets and 99.17%-100%in the testing sets.Fifteen wavelengths(432 nm,455 nm,468 nm,560 nm,705 nm,736 nm,760 nm,841 nm,861 nm,921 nm,930 nm,937 nm,938 nm,959 nm and 965 nm)were recommended by MGS.The results demonstrated that hyperspectral imaging could be used as a non-destructive method to classify mung bean varieties,and MGS was an effective wavelength selection method.展开更多
Soybean is a crop with a long cultivation history that occupies an important position in agricultural production.Soybean mosaic virus disease(SMV)has caused a rapid decline in soybean yields,causing huge losses to the...Soybean is a crop with a long cultivation history that occupies an important position in agricultural production.Soybean mosaic virus disease(SMV)has caused a rapid decline in soybean yields,causing huge losses to the soybean industry,wherefrom its early detec-tion is particularly important.This study proposes a new classification method for the early SMV,dividing its severity into grades 0,1 and 2.In the case of a small number of experi-mental samples of soybeans,this study proposes a combined convolutional neural network and support vector machine(CNN-SVM)method for the early detection of SMV.Experimen-tal results showed that the accuracy of the training set of the CNN-SVM model reached 96.67%,and the accuracy rate of the test set reached 94.17%.The experiment proved the feasibility of using the proposed CNN-SVM model to classify early SMV under the new clas-sification method,and provided a new direction for early SMV detection based on hyper-spectral images.展开更多
Soil total nitrogen content(TN)is a crucial factor in boosting the growth of crops.Its sur-plus or scarcity will alter the quality and yield of crops to a certain extent.Traditional methods such as chemical analysis i...Soil total nitrogen content(TN)is a crucial factor in boosting the growth of crops.Its sur-plus or scarcity will alter the quality and yield of crops to a certain extent.Traditional methods such as chemical analysis is complicated,laborious and time-consuming.A faster and more efficient method to detect TN should be explored to address this problem.The hyperspectral technology integrates conventional energy and spectroscopy which aids in the simultaneous collection of spatial and spectral information from an object.It has grad-ually proved its significance and gained popularity in the analysis of soil composition.This study discussed the possibility of using hyperspectral technology to detect TN,analyzed six spectral data preprocessing methods and five modeling methods:partial least squares(PLS),back-propagation(BP)neural network,radial basis function(RBF)neural network,extreme learning machine(ELM)and support vector regression(SVR)with evaluation index R^(2) and RMSE.Setting the content of chemical analysis as the control and comparing the errors from spectral analysis.According to the results,all five models can be used for TN detection,and the SVR model with R^(2) 0.9121 and RMSE 0.7581 turned to the best method.The study showed that the spectral model can detect TN quickly,providing a reference for the detection of elements in soil with favorable research significance.展开更多
This manuscript presents a preliminary investigation on the applicability of hyperspectral imaging technology for nondestructive and rapid analysis to reveal covered original handwritings.The hyperspectral imager Nuan...This manuscript presents a preliminary investigation on the applicability of hyperspectral imaging technology for nondestructive and rapid analysis to reveal covered original handwritings.The hyperspectral imager Nuance‑Macro was used to collect the reflected light signature of inks from the overlapping parts.The software Nuance1p46 was used to analyze the reflected light signature of inks which shows the covered original handwritings.Different types of black/blue ballpoint pen inks and black/blue gel pen inks were chosen for sample preparation.From the hyperspectral images examined,the covered original handwritings of application were revealed in 90.5%,69.1%,49.5%,and 78.6%of the cases.Further,the correlation between the revealing effect and spectral characteristics of the reflected light of inks at the overlapping parts was interpreted through theoretical analysis and experimental verification.The results indicated that when the spectral characteristics of the reflected light of inks at the overlapping parts were the same or very similar to that of the ink that was used to cover the original handwriting,the original handwriting could not be shown.On the contrary,when the spectral characteristics of the reflected light of inks at the overlapping parts were different to that of the ink that was used to cover the original handwriting,the original handwriting was revealed.展开更多
基金supported by the National Key Research and Development Project of China(2021YFD1600101)the earmarked fund for the China Agriculture Research System(CARS-12 and CARS-13)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2024-OCRI).
文摘Oil content is a crucial indicator for evaluating the quality of peanuts.A rapid and non-destructive method to determine oil content of individual peanut seed can provide robust technical support for breeding high-oil-content peanut varieties.In this study,we established a rapid determination method using near-infrared hyperspectral imaging and chemometrics to assess the oil content of single peanut seed.After selecting key wavelengths through competitive adaptive reweighted sampling(CARS),uninformative variable elimination(UVE),and random frog(RF),we constructed an oil content calibration model based on partial least squares regression for single peanut seed.Validation results demonstrated that the correlation coefficient was 0.8393 with a root mean square error of 1.7771 in the calibration set,while it was 0.7915 with a root mean square error of 2.2943 in the independent prediction set.Most samples exhibited relative errors below 5%,confirming the reliability of this model in predicting oil content of single peanut seed.
基金Supported by the National Key Research and Development Program of China(2016YFD0700204-02)China Agriculture Research System(CARS-36)Heilongjiang Post-doctoral Subsidy Project of China(LBH-Z17020)。
文摘To ensure the quality and safety of pure milk,detection method of typical preservative-potassium sorbate in milk was researched in this paper.Hyperspectral imaging technology was applied to realize rapid detection.Influence factors for hyperspectral data collection for milk samples were firstly researched,including height of sample,bottom color and sample filled up container or not.Pretreatment methods and variable selection algorithms were applied into original spectral data.Rapid detection models were built based on support vector machine method(SVM).Finally,standard normalized variable(SNV)-competitive adaptive reweighted sampling(CARS)and SVM model was chosen in this paper.The accuracies of calibration set and testing set were 0.97 and 0.97,respectively.Kappa coefficient of the model was 0.93.It could be seen that hyperspectral imaging technology could be used to detect for potassium sorbate in milk.Meanwhile,it also provided methodological supports for the rapid detection of other preservatives in milk.
文摘We observed atherosclerotic plaque phantoms using a novel near-infrared (NIR) hyperspectral imaging (HSI) technique. Data were obtained through saline or blood layers to simulate an angioscopic environment for the phantom. For the study, we developed a NIR-HSI system with an NIR supercontinuum light source and mercury-cadmium-telluride camera. Apparent spectral absorbance was obtained at wavelengths of 1150 - 2400 nm. Hyperspectral images of lipid were constructed using a spectral angle mapper algorithm. Bovine fat covered with saline or blood was observed using hyperspectral images at a wavelength around 1200 nm. Our results show that NIR-HSI is a promising angioscopic technique with the potential to identify lipid-rich plaques without clamping and saline injection.
基金This work was supported by Zhejiang Public Welfare Program of Applied Research(LGN19D010001)Zhejiang Agricultural Cooperative and Extensive Project of Key Technology(2020XTTGCY04-02+1 种基金2020XTTGCY01-05)the National Key R&D Program of China(2017YFE0122500).
文摘Tea plant stresses threaten the quality of tea seriously.The technology corresponding to the fast detection and differentiation of stresses is of great significance for plant protection in tea plantation.In recent years,hyperspectral imaging technology has shown great potential in detecting and differentiating plant diseases,pests and some other stresses at the leaf level.However,the lack of studies at canopy level hampers the detection of tea plant stresses at a larger scale.In this study,based on the canopy-level hyperspectral imaging data,the methods for identifying and differentiating the three commonly occurred tea stresses(i.e.,the tea leafhopper,anthrax and sun burn)were studied.To account for the complexity of the canopy scenario,a stepwise detecting strategy was proposed that includes the process of background removal,identification of damaged areas and discrimination of stresses.Firstly,combining the successive projection algorithm(SPA)spectral analysis and K-means cluster analysis,the background and overexposed non-plant regions were removed from the image.Then,a rigorous sensitivity analysis and optimization were performed on various forms of spectral features,which yielded optimal features for detecting damaged areas(i.e.,YSV,Area,GI,CARI and NBNDVI)and optimal features for stresses discrimination(i.e.,MCARI,CI,LCI,RARS,TCI and VOG).Based on this information,the models for identifying damaged areas and those models for discriminating different stresses were established using K-nearest neighbor(KNN),Random Forest(RF)and Fisher discriminant analysis.The identification model achieved an accuracy over 95%,and the discrimination model achieved an accuracy over 93%for all stresses.The results suggested the feasibility of stress detection and differentiation using canopy-level hyperspectral imaging techniques,and indicated the potential for its extension over large areas.
基金supported by the National Natural Science Foundation of China 62175153the Shanghai Science and Technology Commission 21S902700.
文摘Objective:We applied hyperspectral imaging(HSI)system to distinguish early caries from soundand pigmented areas.It will provide a theoretical basis and technical support,for research anddevelopment of an instrument that could be used for screening and detection of early dentalcaries.Methods:Eighteen extracted human teeth(molars and premolars),with varying degrees ofnatural pathology and no degree of decay involving dentin were obtained.HSI system with awavelength range from 400 to 1000nm was used to obtain images of all 18 teeth containingsound,carious and pigmented areas.We compared the spectra of the wavebands at both 500 nmand 780 nm from the different tooth states,and the reflectance diference bet ween sound versuscarious lesions and sound versus pigmented areas,respectively.Results:There was a slight diference in refectance bet ween carious areas and pigmented areas at500 nm.A substantial difference was additionally noted in refectance bet ween carious areas andpigmented areas at 780 nm.Conclusion:The results have shown that the interference of tooth surface pigment can be elim-inated in the near-infrared(NIR)waveband,and the caries can be effectively identifed from the pigmented areas.Thus,it could be used to detect carious areas of teeth in place of the traditionalvisual inspection method or white light endoscopy.Clinical significance:The NIR difused light signal enables the identification of early caries frompigment and other interference,providing a reasonable detection tool for early detection andearly treatment of teeth diseases.
基金the State Administration of Traditional Chinese Medicine of Zhejiang Province Project(No.2015ZQ022)the Zhejiang TCM Health Science and Technology Project(No.2015KYB110).
文摘As unsafe components in herbal medicine(HM),saccharides can affect not only the drug appearance and stabilization,but also the drug efficacy and safety.The present study focuses on the in-line monitoring of batch alcohol precipitation processes for saccharide removal using nearinfrared(NIR)spectroscopy.NIR spectra in the 4000–10,000-cm^(-1)wavelength range are acquired in situ using a transflectance probe.These directly acquired spectra allow characterization of the dynamic variation tendency of saccharides during alcohol precipitation.Calibration models based on partial least squares(PLS)regression have been developed for the three saccharide impurities,namely glucose,fructose,and sucrose.Model errors are estimated as the root-meansquare errors of cross-validation(RMSECVs)of internal validation and root-mean-square errors of prediction(RMSEPs)of external validation.The RMSECV values of glucose,fructose,and sucrose were 1.150,1.535,and 3.067 mg·mL^(-1),and the RMSEP values were 0.711,1.547,and 3.740 mg·mL^(-1),respectively.The correlation coeffcients(r)between the NIR predictive and the reference measurement values were all above 0.94.Furthermore,NIR predictions based on the constructed models improved our understanding of sugar removal and helped develop a control strategy for alcohol precipitation.The results demonstrate that,as an alternative process analytical technology(PAT)tool for monitoring batch alcohol precipitation processes,NIR spectroscopy is advantageous for both efficient determination of quality characteristics(fast,in situ,and requiring no toxic reagents)and process stability,and evaluating the repeatability.
文摘Hyperspectral imaging is gaining a significant role in agricultural remote sensing applications.Its data unit is the hyperspectral cube which holds spatial information in two dimensions while spectral band information of each pixel in the third dimension.The classification accuracy of hyperspectral images(HSI)increases significantly by employing both spatial and spectral features.For this work,the data was acquired using an airborne hyperspectral imager system which collected HSI in the visible and near-infrared(VNIR)range of 400 to 1000 nm wavelength within 180 spectral bands.The dataset is collected for nine different crops on agricultural land with a spectral resolution of 3.3 nm wavelength for each pixel.The data was cleaned from geometric distortions and stored with the class labels and annotations of global localization using the inertial navigation system.In this study,a unique pixel-based approach was designed to improve the crops'classification accuracy by using the edge-preserving features(EPF)and principal component analysis(PCA)in conjunction.The preliminary processing generated the high-dimensional EPF stack by applying the edge-preserving filters on acquired HSI.In the second step,this high dimensional stack was treated with the PCA for dimensionality reduction without losing significant spectral information.The resultant feature space(PCA-EPF)demonstrated enhanced class separability for improved crop classification with reduced dimensionality and computational cost.The support vector machines classifier was employed for multiclass classification of target crops using PCA-EPF.The classification performance evaluation was measured in terms of individual class accuracy,overall accuracy,average accuracy,and Cohen kappa factor.The proposed scheme achieved greater than 90%results for all the performance evaluation metrics.The PCA-EPF proved to be an effective attribute for crop classification using hyperspectral imaging in the VNIR range.The proposed scheme is well-suited for practical applications of crops and landfill estimations using agricultural remote sensing methods.
基金supported by the National Key Research and Development Program of China(2021YFD2000100)the National Natural Science Foundation of China(32101617)+3 种基金the Fundamental Research Funds for the Central Universities(JSJL2023005)the Zhongshan Biological Breeding Laboratory(ZSBBL-KY2023-05)the Key Independent Research Project of Jjiangsu Key Laboratory of Information Agriculture(KLIAZZ2301)the Jiangsu Collaborative Innovation Center for Modern Crop Production(JCICMCP).
文摘Efficient and accurate acquisition of the rice grain protein content(GPC)is important for selecting high-quality rice varieties,and remote sensing technology is an attractive potential method for this task.However,the majority of multispectral sensors are poor predictors of GPC due to their broad spectral bands.Hyperspectral technology provides a new analytical technology for bridging the gap between phenomics and genomics.However,the small size of typical datasets is a constraint for model construction for estimating GPC,limiting their accuracy and reducing their ability to generalize to a wide range of varieties.In this study,we used hyperspectral data of rice grains from 515 japonica varieties and deep convolution generative adversarial networks(DCGANs)to generate simulated data to improve the model accuracy.Features sensitive to GPC were extracted after applying a continuous wavelet transform(CWT),and the estimated GPC model was constructed by partial least squares regression(PLSR).Finally,a genome-wide association study(GWAS)was applied to the measured and generated datasets to detect GPC loci.The results demonstrated that the simulated GPC values generated after 8,000 epochs were closest to the measured values.The wavelet feature(WF_(1743,2)),obtained from the data with the addition of 200 simulated samples,exhibited the highest GPC estimation accuracy(R^(2)=0.58 and RRMSE=6.70%).The GWAS analysis showed that the estimated values based on the simulated data detected the same loci as the measured values,including the OsmtSSB1L gene related to grain storage protein.This study provides a new technique for the efficient genetic study of phenotypic traits in rice based on hyperspectral technology.
基金Jiangxi Provincial Administration of Traditional Chinese Medicine Key Research Laboratory on the Fundamentals of Chinese Medicine Evidence(Gan TCM Science and Education Word[2022]No.8-4)Jiangxi University of Chinese Medicine Science and Technology Innovation Team Development Program:Traditional Chinese Medicine Constitution-State Identification Health Management Research Team(No.CXTD22016)。
文摘OBJECTIVE:To evaluate the quality of Moyao(Myrrh)in the identification of the geographical origin and processing of the products.METHODS:Raw Moyao(Myrrh)and two kinds of Moyao(Myrrh)processed with vinegar from three countries were identified using near-infrared(NIR)spectroscopy combined with chemometric techniques.Principal component analysis(PCA)was used to reduce the dimensionality of the data and visualize the clustering of samples from different categories.A classical chemometric algorithm(PLS-DA)and two machine learning algorithms[K-nearest neighbor(KNN)and support vector machine]were used to conduct a classification analysis of the near-infrared spectra of the Moyao(Myrrh)samples,and their discriminative performance was evaluated.RESULTS:Based on the accuracy,precision,recall rate,and F1 value in each model,the results showed that the classical chemometric algorithm and the machine learning algorithm obtained positive results.In all of the chemometric analyses,the NIR spectrum of Moyao(Myrrh)preprocessed by standard normal variation or Multivariate scattering correction combined with KNN achieved the highest accuracy in identifying the geographical origins,and the accuracy of identifying the processing technology established by the KNN method after first-order derivative pretreatment was the best.The best accuracy of geographical origin discrimination and processing technology discrimination were 0.9853 and 0.9706 respectively.CONCLUSIONS:NIR spectroscopy combined with chemometric technology can be an important tool for tracking the origin and processing technology of Moyao(Myrrh)and can also provide a reference for evaluations of its quality and the clinical use.
基金This work was supported by the National Key Scientific Instrument and Equipment Development Projects(2014YQ470377)the Scientific Research Foundation for Returned Overseas Students and the Fundamental Research Funds for the Central Universities of China(2012FZA6005,2013QNA6011).
文摘This study was carried out to investigate the feasibility of using visible and near infrared hyperspectral imaging for the variety classification of mung beans.Raw hyperspectral images of mung beans were acquired in the wavelengths of 380-1023 nm,and all images were calibrated by the white and dark reference images.The spectral reflectance values were extracted from the region of interest(ROI)of each calibrated hyperspectral image,and then they were treated as the independent variables.The dependent variables of four varieties of mung beans were set as 1,2,3 and 4,respectively.The extreme learning machine(ELM)model was established using full spectral wavelengths for classification.Modified gram-schmidt(MGS)method was used to identify effective wavelengths.Based on the selected wavelengths,the ELM and linear discriminant analysis(LDA)models were built.All models performed excellently with the correct classification rates(CCRs)covering 99.17%-99.58% in the training sets and 99.17%-100%in the testing sets.Fifteen wavelengths(432 nm,455 nm,468 nm,560 nm,705 nm,736 nm,760 nm,841 nm,861 nm,921 nm,930 nm,937 nm,938 nm,959 nm and 965 nm)were recommended by MGS.The results demonstrated that hyperspectral imaging could be used as a non-destructive method to classify mung bean varieties,and MGS was an effective wavelength selection method.
基金This work is supported by National Natural Science Founda-tion of China(NSFC)(32071904)。
文摘Soybean is a crop with a long cultivation history that occupies an important position in agricultural production.Soybean mosaic virus disease(SMV)has caused a rapid decline in soybean yields,causing huge losses to the soybean industry,wherefrom its early detec-tion is particularly important.This study proposes a new classification method for the early SMV,dividing its severity into grades 0,1 and 2.In the case of a small number of experi-mental samples of soybeans,this study proposes a combined convolutional neural network and support vector machine(CNN-SVM)method for the early detection of SMV.Experimen-tal results showed that the accuracy of the training set of the CNN-SVM model reached 96.67%,and the accuracy rate of the test set reached 94.17%.The experiment proved the feasibility of using the proposed CNN-SVM model to classify early SMV under the new clas-sification method,and provided a new direction for early SMV detection based on hyper-spectral images.
基金This study was funded by the National Key Technologies Research and Development Program“Ecology Safety Assur-ance Technology for Coalx Basein Northwest Arid DesertArea”(2017YFC0504400)the National Natural Science Foundation of China(31770769)the Fundamental Research Funds for the Central Universities(NO.2015ZCQ-GX-03)。
文摘Soil total nitrogen content(TN)is a crucial factor in boosting the growth of crops.Its sur-plus or scarcity will alter the quality and yield of crops to a certain extent.Traditional methods such as chemical analysis is complicated,laborious and time-consuming.A faster and more efficient method to detect TN should be explored to address this problem.The hyperspectral technology integrates conventional energy and spectroscopy which aids in the simultaneous collection of spatial and spectral information from an object.It has grad-ually proved its significance and gained popularity in the analysis of soil composition.This study discussed the possibility of using hyperspectral technology to detect TN,analyzed six spectral data preprocessing methods and five modeling methods:partial least squares(PLS),back-propagation(BP)neural network,radial basis function(RBF)neural network,extreme learning machine(ELM)and support vector regression(SVR)with evaluation index R^(2) and RMSE.Setting the content of chemical analysis as the control and comparing the errors from spectral analysis.According to the results,all five models can be used for TN detection,and the SVR model with R^(2) 0.9121 and RMSE 0.7581 turned to the best method.The study showed that the spectral model can detect TN quickly,providing a reference for the detection of elements in soil with favorable research significance.
基金supported by Program for Young Innovative Research Team in China University of Political Science and Law(1000‑10814344)Young Research in China University of Political Science and Law(16ZFQ82008).
文摘This manuscript presents a preliminary investigation on the applicability of hyperspectral imaging technology for nondestructive and rapid analysis to reveal covered original handwritings.The hyperspectral imager Nuance‑Macro was used to collect the reflected light signature of inks from the overlapping parts.The software Nuance1p46 was used to analyze the reflected light signature of inks which shows the covered original handwritings.Different types of black/blue ballpoint pen inks and black/blue gel pen inks were chosen for sample preparation.From the hyperspectral images examined,the covered original handwritings of application were revealed in 90.5%,69.1%,49.5%,and 78.6%of the cases.Further,the correlation between the revealing effect and spectral characteristics of the reflected light of inks at the overlapping parts was interpreted through theoretical analysis and experimental verification.The results indicated that when the spectral characteristics of the reflected light of inks at the overlapping parts were the same or very similar to that of the ink that was used to cover the original handwriting,the original handwriting could not be shown.On the contrary,when the spectral characteristics of the reflected light of inks at the overlapping parts were different to that of the ink that was used to cover the original handwriting,the original handwriting was revealed.