Fluorescence imaging through the second near-infrared window(NIR-Ⅱ,1000–1700 nm) allows in-depth imaging.However, current imaging systems use wide-field illumination and can only provide low-contrast 2D information,...Fluorescence imaging through the second near-infrared window(NIR-Ⅱ,1000–1700 nm) allows in-depth imaging.However, current imaging systems use wide-field illumination and can only provide low-contrast 2D information, without depth resolution. Here, we systematically apply a light-sheet illumination, a time-gated detection, and a deep-learning algorithm to yield high-contrast high-resolution volumetric images. To achieve a large Fo V(field of view) and minimize the scattering effect, we generate a light sheet as thin as 100.5 μm with a Rayleigh length of 8 mm to yield an axial resolution of 220 μm. To further suppress the background, we time-gate to only detect long lifetime luminescence achieving a high contrast of up to 0.45 Icontrast. To enhance the resolution, we develop an algorithm based on profile protrusions detection and a deep neural network and distinguish vasculature from a low-contrast area of 0.07 Icontrast to resolve the 100μm small vessels. The system can rapidly scan a volume of view of 75 × 55 × 20 mm3and collect 750 images within 6mins. By adding a scattering-based modality to acquire the 3D surface profile of the mice skin, we reveal the whole volumetric vasculature network with clear depth resolution within more than 1 mm from the skin. High-contrast large-scale 3D animal imaging helps us expand a new dimension in NIR-Ⅱ imaging.展开更多
Combining phototherapy and chemotherapy has been considered a promising modality for cancer therapy due to their synergistic effect.Herein,we developed three D-π-A-structured boron dipyrromethenes(BODIPYs)(named as B...Combining phototherapy and chemotherapy has been considered a promising modality for cancer therapy due to their synergistic effect.Herein,we developed three D-π-A-structured boron dipyrromethenes(BODIPYs)(named as B-B,B-C,and B-C-Pt).Due to their enlargedπ-conjugated structure and high intramolecular charge transfer effect,the synthesized BODIPYs had photothermal conversion capability,and their absorption and fluorescence spectra were red-shifted.The cisplatin-appended BODIPY(B-C-Pt)exhibited good singlet oxygen(^1O_(2))generation ability and near infrared(NIR)absorption and fluorescence(λ_(Abs)=748 nm,λ_(Em)=947 nm).After being encapsulated by distearoyl phosphoethanolamine polyethyleneglycol 2000(DSPE-PEG-2000),which could inhibit the H-aggregation of B-C-Pt,the absorption and fluorescence of the obtained B-C-Pt nanoparticles(NPs)were red-shifted to 762 and 985 nm,respectively.The^1O_(2)quantum yield and photothermal conversion efficiency of the B-C-Pt NPs were 4.0%and 40.6%,respectively.Moreover,B-C-Pt NPs had chemotherapeutic efficacy due to the presence of cisplatin.In vitro and in vivo studies further demonstrated that B-C-Pt NPs had synergistic therapeutic efficacy.Together,B-C-Pt NPs could be employed in NIRⅡfluorescent and photoacoustic imaging-guided synergistic phototherapy and chemotherapy for cancer treatment.展开更多
Various phototheranostics have recently been developed for phototherapy.Through proper molecular design,the photochemical and photophysical properties of these phototheranostics can be promoted.Herein,an acceptor-dono...Various phototheranostics have recently been developed for phototherapy.Through proper molecular design,the photochemical and photophysical properties of these phototheranostics can be promoted.Herein,an acceptor-donor-acceptor(A-D-A)-structured dye,BTP-4F-DMO,was synthesized and prepared into water-soluble nanoparticles(NPs).The obtained BTP-4F-DMO NPs had strong absorption from650 nm to 850 nm and a fluorescence emission peak at~900 nm that tailed to~1100 nm.The NPs showed a superhigh photothermal conversion efficiency of 90.5%±5%and could simultaneously generate·OH and^(1)O_(2)with a^(1)O_(2)generation quantum yield of 4.6%under 808 nm laser irradiation.Due to these advanced properties,BTP-4F-DMO NPs can switch the role of autophagy from pro-survival to prodeath,thereby further promoting cancer cell death.These features make BTP-4F-DMO NPs a promising multifunctional phototheranostic agent for NIR-II fluorescence/photoacoustic dual-mode imaging-guided synergetic photodynamic/photothermal therapy.In general,this work provides a strategy for expanding the biomedical applications of organic A-D-A-structured phototheranostics.展开更多
Multimodal imaging in the second near-infrared window(NIR-II)guided cancer therapy is a highly precise and efficient cancer theranostic strategy.However,it is still a challenge to develop activated NIR-II optical imag...Multimodal imaging in the second near-infrared window(NIR-II)guided cancer therapy is a highly precise and efficient cancer theranostic strategy.However,it is still a challenge to develop activated NIR-II optical imaging and therapy agents.In this study,we develop a pH-responsive hybrid plasmonic-fluorescent vesicle by self-assembly of amphiphilic plasmonic nanogapped gold nanorod(AuNNR)and fluorescent down-conversion nanoparticles(DCNP)(AuNNR-DCNP Ve),showing remarkable and activated NIR-II fluorescence(FL)/NIR-II photoacoustic(PA)imaging performances.The hybrid vesicle also exhibited superior loading capacity of doxorubicin as a superior drug carrier and efficient radiosensitizer for X-ray-induced radiotherapy.Interestingly,the accumulated hybrid AuNNR-DCNP Ve in the tumor resulted in a recovery of NIR-II FL imaging signal and a variation in NIR-II PA imaging signal.Dual activated NIR-II PA and FL imaging of the hybrid vesicle could trace drug release and precisely guided cancer radiotherapy to ultimately reduce the side effects to healthy tissue.展开更多
Monitoring mitochondrial derived copper（Ⅱ） in live cells is highly demanded, but accurately detecting is unmet due to the interference with cytoplasmic copper（Ⅱ）. Herein, we have reported the design,synthesis an...Monitoring mitochondrial derived copper（Ⅱ） in live cells is highly demanded, but accurately detecting is unmet due to the interference with cytoplasmic copper（Ⅱ）. Herein, we have reported the design,synthesis and characterization of photocontrollable fluorogenic probe, MCu-3, which is equipped with a photo-labile group（nitrobenzyl group） and mitochondria targeting unit（triphenylphosphonium salt）.This novel probe showed an intense fluorescence enhancement in response to copper（Ⅱ） without interference from other metal cations in the biological condition（p H 6–9）. The detection limit is 1.7 ×10^（-7） mol/L in HEPES buffer. The confocal fluorescence imaging results demonstrated MCu-3 can visualize mitochondrial copper（Ⅱ） in live mammalian cells. The clear advantage of our photocontrollable method is successful to avoid the influence of cytoplasmic copper（Ⅱ） during mitochondria specific detection.展开更多
Phototheranostic with highly integrated functions is an attractive platform for cancer management. It remains challenging to develop a facile phototheranostic platform with complementary bimodal imaging and combinatio...Phototheranostic with highly integrated functions is an attractive platform for cancer management. It remains challenging to develop a facile phototheranostic platform with complementary bimodal imaging and combinational therapy capacity. Herein, the small-molecule cyanine IR780 loaded liposomes have been harnessed as a nanoplatform to simultaneously realize photoacoustic(PA)/the second near-infrared window(NIR-Ⅱ) fluorescence imaging and image-guided surgery/adjuvant photothermal therapy(PTT).This nanoplatform exhibits attractive properties like uniform controllable size, stable dispersibility, NIR-Ⅱ fluorescence emission, photothermal conversion, and biocompatibility. Benefiting from the complementary PA/NIR-Ⅱ fluorescence bimodal imaging, this nanoplatform was successfully applied in precise vasculature delineation and tumor diagnosis. Interestingly, the tumor was clearly detected by NIR-Ⅱ fluorescence imaging with the highest tumor-to-normal-tissue ratio up to 12.69, while signal interference from the liver was significantly reduced, due to the difference in the elimination rate of the nanoplatform in the liver and tumor. Under the precise guidance of the image, the tumor was accurately resected, and the simulated residual lesion after surgery was completely ablated by adjuvant PTT. This combined therapy showed improved antitumor efcacy over the individual surgery or PTT. This work develops a facile phototheranostic nanoplatform with great significance in accurately diagnosing and effectively treating tumors using simple NIR light irradiation.展开更多
Ovarian cancer is a global problem,and is typically diagnosed in the middle or late stages,with a mysterious abdominal mass or atypical abdominal metastases due to the lack of specific initial diagnostic methods.Dual-...Ovarian cancer is a global problem,and is typically diagnosed in the middle or late stages,with a mysterious abdominal mass or atypical abdominal metastases due to the lack of specific initial diagnostic methods.Dual-modal near-infrared Ⅱ(NIR-Ⅱ,1,000–1,700 nm)fluorescence/photoacoustic imaging has great potential in early ovarian cancer diagnosis and image-guided surgery due to its high sensitivity and deep penetration.Herein,we report a novel organic NIR-Ⅱ dye(H10)with excellent aggregation-induced-emission(AIE)characteristics(I/I0>1.6)utilizing a selenadiazolo-[3,4-f]benzo[c][1,2,5]thiadiazole(ST)-based building block.Then,water-soluble and biocompatible H10@follicle-stimulating hormone(H10@FSH)dots with superior optical/photoacoustic properties and a tenfold increase in ovarian-specific targeting ability were synthesized.Finally,for the first time,in vivo dual-mode NIR-Ⅱ fluorescent/photoacoustic(PA)imaging and image-guided surgery of patient-derived tumor xenograft(PDTX)and micro-metastatic abdominal ovarian cancer lesions were investigated.This novel strategy will establish a new method for early detection of ovarian cancer and significantly improve the prognosis of ovarian cancer patients.展开更多
Gold nanoclusters(AuNCs)with near-infraredⅡ(NIR-Ⅱ)photoluminescence(PL)have emerged as novel bioimaging probes for in vivo disease diagnosis.So far,it still lacks a systematic review focusing on the synthesis,PL tun...Gold nanoclusters(AuNCs)with near-infraredⅡ(NIR-Ⅱ)photoluminescence(PL)have emerged as novel bioimaging probes for in vivo disease diagnosis.So far,it still lacks a systematic review focusing on the synthesis,PL tuning,and in vivo imaging of NIR-Ⅱluminescent AuNCs.In this review,we briefly introduce the synthesis of NIR-Ⅱluminescent AuNCs using various surface ligands.We discuss the origins and properties of NIR-ⅡPL in AuNCs,and summarize the strategies for improving and/or tuning NIR-ⅡPL emissions.We also provide an overview of the recent progress in the application of AuNCs in tumor-targeted imaging,molecular imaging,and other areas(such as the sensitive imaging of bones,vessels,lymph nodes,etc.).Finally,we present the prospects and challenges in the field of NIR-Ⅱluminescent AuNCs and related imaging applications,expecting to offer comprehensive understanding of this field,and thereby deepening and broadening the biological application of AuNCs.展开更多
近红外二区(900~1 880 nm,the Second Near-Infrared Region,NIR-Ⅱ)荧光宽场显微成像技术是当前大深度活体成像的一大研究热点,在基础研究和临床应用方面都拥有巨大的潜力。对比可见光(360~760 nm)和近红外一区(760~900 nm,the First N...近红外二区(900~1 880 nm,the Second Near-Infrared Region,NIR-Ⅱ)荧光宽场显微成像技术是当前大深度活体成像的一大研究热点,在基础研究和临床应用方面都拥有巨大的潜力。对比可见光(360~760 nm)和近红外一区(760~900 nm,the First Near-Infrared Region,NIR-Ⅰ)的成像,NIR-Ⅱ荧光宽场显微成像技术在活体层面具有更高的清晰度和更深的组织穿透。在NIR-Ⅱ宏观成像基础上,对组织微结构清晰成像的需求迫使成像试剂持续发展,成像系统不断精进。目前,NIR-Ⅱ荧光宽场显微成像技术在脉管显微造影、肿瘤精确分析、炎症准确追踪等生物应用上都获得一系列突破,相关研究对象包含啮齿类动物(如小鼠,大鼠)及灵长类动物(如狨猴,猕猴)等。将来随着仪器商业化和国产化突破,成像试剂安全性逐步提高,NIR-Ⅱ荧光宽场显微成像应用价值将不断攀升。本文从NIR-Ⅱ荧光成像的机制及优势展开讨论,综述NIR-Ⅱ荧光宽场显微成像的系统特点和演进历史,以及其在不同生物模型上活体成像方面的最新探索和前景展望,以期推动NIR-Ⅱ荧光宽场显微成像技术进一步普及。展开更多
Although metal-based chemical agents have demonstrated promising bacteriostatic effects in phototherapy,their short excitation/emission wavelengths and inadequate phototherapy efficiencies make their application in vi...Although metal-based chemical agents have demonstrated promising bacteriostatic effects in phototherapy,their short excitation/emission wavelengths and inadequate phototherapy efficiencies make their application in vivo difficult.We therefore synthesized a novel Pt(Ⅱ)metallacycle(Pt1110)that can be activated with a 980 nm laser for photodiagnosis/treatment in deep tissue.We found that Pt1110 significantly improved photothermal conversion(95%improvement)and ^(1)O_(2) generation(ΦΔ75%increase)compared to the ligand itself 1 and was well capable of light-induced sterilization under safe laser irradiation(0.72 W/cm^(2)).In addition,Pt1110 has little to no toxicity to cells.After incorporated into liposome,Pt1110 NPs was effective in wound healing in infection and keratitis models upon laser irradiation,which was accurately observed by NIR-Ⅱfluorescence imaging.This novel metal-coordinated supramolecular material has a potential to become a universal platform for phototherapy in deep tissue.展开更多
Detection of mercury ions(Hg^(2+))in actual samples is of significant importance due to the toxicity of Hg^(2+)to human health.In this work,a simple tetraphenylethene(TPE)derived fluorescent probe TPE-Hg based on aggr...Detection of mercury ions(Hg^(2+))in actual samples is of significant importance due to the toxicity of Hg^(2+)to human health.In this work,a simple tetraphenylethene(TPE)derived fluorescent probe TPE-Hg based on aggregation-induced emission(AIE)mechanism was synthesized.TPE-Hg can visually recognize Hg^(2+)in THF/HEPES(1:9,v/v,HEPES 20 mmol/L,pH 7.3)system with rapid response,strong anti-interference ability,large Stokes shift(203 nm),and low detection limit(7.548×10^(-7)mol/L).The results show that Hg^(2+)triggered elimination of TPE-Hg lead to releasing of an AIE-active compound 2 is responsible to the sensing mechanism.TPE-Hg is applicable to detect Hg^(2+)in actual water samples and image Hg^(2+)in living MCF-7 cells.In addition,TPE-Hg is suitable to assay the Hg^(2+)level in seafood and tea samples,and it is alsoapplicable intest strips.展开更多
基金Technology Program(KQTD20170810110913065,20200925174735005)National Natural Science Foundation of China(62005116,51720105015)Guangdong Provincial Key Laboratory of Advanced Biomaterials(2022B1212010003).
文摘Fluorescence imaging through the second near-infrared window(NIR-Ⅱ,1000–1700 nm) allows in-depth imaging.However, current imaging systems use wide-field illumination and can only provide low-contrast 2D information, without depth resolution. Here, we systematically apply a light-sheet illumination, a time-gated detection, and a deep-learning algorithm to yield high-contrast high-resolution volumetric images. To achieve a large Fo V(field of view) and minimize the scattering effect, we generate a light sheet as thin as 100.5 μm with a Rayleigh length of 8 mm to yield an axial resolution of 220 μm. To further suppress the background, we time-gate to only detect long lifetime luminescence achieving a high contrast of up to 0.45 Icontrast. To enhance the resolution, we develop an algorithm based on profile protrusions detection and a deep neural network and distinguish vasculature from a low-contrast area of 0.07 Icontrast to resolve the 100μm small vessels. The system can rapidly scan a volume of view of 75 × 55 × 20 mm3and collect 750 images within 6mins. By adding a scattering-based modality to acquire the 3D surface profile of the mice skin, we reveal the whole volumetric vasculature network with clear depth resolution within more than 1 mm from the skin. High-contrast large-scale 3D animal imaging helps us expand a new dimension in NIR-Ⅱ imaging.
基金supported by National Natural Science Foundation of China(No.62175262)the Science and Technology Innovation Program of Hunan Province(No.2022RC1201)the Fundamental Research Funds for the Central Universities of Central South University(No.2019zzts849)。
文摘Combining phototherapy and chemotherapy has been considered a promising modality for cancer therapy due to their synergistic effect.Herein,we developed three D-π-A-structured boron dipyrromethenes(BODIPYs)(named as B-B,B-C,and B-C-Pt).Due to their enlargedπ-conjugated structure and high intramolecular charge transfer effect,the synthesized BODIPYs had photothermal conversion capability,and their absorption and fluorescence spectra were red-shifted.The cisplatin-appended BODIPY(B-C-Pt)exhibited good singlet oxygen(^1O_(2))generation ability and near infrared(NIR)absorption and fluorescence(λ_(Abs)=748 nm,λ_(Em)=947 nm).After being encapsulated by distearoyl phosphoethanolamine polyethyleneglycol 2000(DSPE-PEG-2000),which could inhibit the H-aggregation of B-C-Pt,the absorption and fluorescence of the obtained B-C-Pt nanoparticles(NPs)were red-shifted to 762 and 985 nm,respectively.The^1O_(2)quantum yield and photothermal conversion efficiency of the B-C-Pt NPs were 4.0%and 40.6%,respectively.Moreover,B-C-Pt NPs had chemotherapeutic efficacy due to the presence of cisplatin.In vitro and in vivo studies further demonstrated that B-C-Pt NPs had synergistic therapeutic efficacy.Together,B-C-Pt NPs could be employed in NIRⅡfluorescent and photoacoustic imaging-guided synergistic phototherapy and chemotherapy for cancer treatment.
基金supported by the National Natural Science Foundation of China(Nos.61805287 and 62175262)the Innovation-Driven Project of Central South University(No.2020CX021)。
文摘Various phototheranostics have recently been developed for phototherapy.Through proper molecular design,the photochemical and photophysical properties of these phototheranostics can be promoted.Herein,an acceptor-donor-acceptor(A-D-A)-structured dye,BTP-4F-DMO,was synthesized and prepared into water-soluble nanoparticles(NPs).The obtained BTP-4F-DMO NPs had strong absorption from650 nm to 850 nm and a fluorescence emission peak at~900 nm that tailed to~1100 nm.The NPs showed a superhigh photothermal conversion efficiency of 90.5%±5%and could simultaneously generate·OH and^(1)O_(2)with a^(1)O_(2)generation quantum yield of 4.6%under 808 nm laser irradiation.Due to these advanced properties,BTP-4F-DMO NPs can switch the role of autophagy from pro-survival to prodeath,thereby further promoting cancer cell death.These features make BTP-4F-DMO NPs a promising multifunctional phototheranostic agent for NIR-II fluorescence/photoacoustic dual-mode imaging-guided synergetic photodynamic/photothermal therapy.In general,this work provides a strategy for expanding the biomedical applications of organic A-D-A-structured phototheranostics.
基金This work was supported by the National Natural Science Foundation of China(Nos.21635002 and 21874024)the joint research projects of Health and Education Commission of Fujian Province(No.2019-WJ-20).
文摘Multimodal imaging in the second near-infrared window(NIR-II)guided cancer therapy is a highly precise and efficient cancer theranostic strategy.However,it is still a challenge to develop activated NIR-II optical imaging and therapy agents.In this study,we develop a pH-responsive hybrid plasmonic-fluorescent vesicle by self-assembly of amphiphilic plasmonic nanogapped gold nanorod(AuNNR)and fluorescent down-conversion nanoparticles(DCNP)(AuNNR-DCNP Ve),showing remarkable and activated NIR-II fluorescence(FL)/NIR-II photoacoustic(PA)imaging performances.The hybrid vesicle also exhibited superior loading capacity of doxorubicin as a superior drug carrier and efficient radiosensitizer for X-ray-induced radiotherapy.Interestingly,the accumulated hybrid AuNNR-DCNP Ve in the tumor resulted in a recovery of NIR-II FL imaging signal and a variation in NIR-II PA imaging signal.Dual activated NIR-II PA and FL imaging of the hybrid vesicle could trace drug release and precisely guided cancer radiotherapy to ultimately reduce the side effects to healthy tissue.
基金supported by the National Natural Science Foundation of China (Nos. 81672508, 61505076)Natural Science Foundation of Jiangsu Province (No. BK20140951)+1 种基金Key University Science Research Project of Jiangsu Province (No. 16KJA180004)SICAM Fellowship & Scholarship by Jiangsu National Synergetic Innovation Center for Advanced Materials
文摘Monitoring mitochondrial derived copper（Ⅱ） in live cells is highly demanded, but accurately detecting is unmet due to the interference with cytoplasmic copper（Ⅱ）. Herein, we have reported the design,synthesis and characterization of photocontrollable fluorogenic probe, MCu-3, which is equipped with a photo-labile group（nitrobenzyl group） and mitochondria targeting unit（triphenylphosphonium salt）.This novel probe showed an intense fluorescence enhancement in response to copper（Ⅱ） without interference from other metal cations in the biological condition（p H 6–9）. The detection limit is 1.7 ×10^（-7） mol/L in HEPES buffer. The confocal fluorescence imaging results demonstrated MCu-3 can visualize mitochondrial copper（Ⅱ） in live mammalian cells. The clear advantage of our photocontrollable method is successful to avoid the influence of cytoplasmic copper（Ⅱ） during mitochondria specific detection.
基金financially supported in part by the National Natural Science Foundation of China(Nos.62075103,81973488)the Natural Science Foundation of Jiangsu Province(No.BK20211271)+4 种基金the Foundation of Anhui Province Key Laboratory of Pharmaceutical Preparation Technology and Application(No.2021KFKT07)the Training Program of Innovation and Entrepreneurship for College Students in Jiangsu(No.202110312037Z)the Jiangsu Provincial Medical Youth Talent(No.QNRC2016075)the Nanjing Medical Science and Technique Development Foundation(No.ZKX19022)the Jiangsu Provincial High level Health Talent“six one project”(No.LGY2019005)。
文摘Phototheranostic with highly integrated functions is an attractive platform for cancer management. It remains challenging to develop a facile phototheranostic platform with complementary bimodal imaging and combinational therapy capacity. Herein, the small-molecule cyanine IR780 loaded liposomes have been harnessed as a nanoplatform to simultaneously realize photoacoustic(PA)/the second near-infrared window(NIR-Ⅱ) fluorescence imaging and image-guided surgery/adjuvant photothermal therapy(PTT).This nanoplatform exhibits attractive properties like uniform controllable size, stable dispersibility, NIR-Ⅱ fluorescence emission, photothermal conversion, and biocompatibility. Benefiting from the complementary PA/NIR-Ⅱ fluorescence bimodal imaging, this nanoplatform was successfully applied in precise vasculature delineation and tumor diagnosis. Interestingly, the tumor was clearly detected by NIR-Ⅱ fluorescence imaging with the highest tumor-to-normal-tissue ratio up to 12.69, while signal interference from the liver was significantly reduced, due to the difference in the elimination rate of the nanoplatform in the liver and tumor. Under the precise guidance of the image, the tumor was accurately resected, and the simulated residual lesion after surgery was completely ablated by adjuvant PTT. This combined therapy showed improved antitumor efcacy over the individual surgery or PTT. This work develops a facile phototheranostic nanoplatform with great significance in accurately diagnosing and effectively treating tumors using simple NIR light irradiation.
基金supported by the National Key Research and development Program of China(Nos.2020YFA0908800 and 2015DFA30440)the National Natural Science Foundation of China(Nos.82111530209,81773674,81573383,91959103,and 61971447)+4 种基金Hubei Province Scientific and Technical Innovation Key Project(No.2020BAB058)Beijing Natural Science Foundation(No.JQ18023)Shenzhen Science and Technology Research Grant(No.JCYJ20190808152019182)the Local Development Funds of Science and Technology Department of Tibet(Nos.XZ202102YD0033C and XZ202202YD0021C)the Fundamental Research Funds for the Central Universities.
文摘Ovarian cancer is a global problem,and is typically diagnosed in the middle or late stages,with a mysterious abdominal mass or atypical abdominal metastases due to the lack of specific initial diagnostic methods.Dual-modal near-infrared Ⅱ(NIR-Ⅱ,1,000–1,700 nm)fluorescence/photoacoustic imaging has great potential in early ovarian cancer diagnosis and image-guided surgery due to its high sensitivity and deep penetration.Herein,we report a novel organic NIR-Ⅱ dye(H10)with excellent aggregation-induced-emission(AIE)characteristics(I/I0>1.6)utilizing a selenadiazolo-[3,4-f]benzo[c][1,2,5]thiadiazole(ST)-based building block.Then,water-soluble and biocompatible H10@follicle-stimulating hormone(H10@FSH)dots with superior optical/photoacoustic properties and a tenfold increase in ovarian-specific targeting ability were synthesized.Finally,for the first time,in vivo dual-mode NIR-Ⅱ fluorescent/photoacoustic(PA)imaging and image-guided surgery of patient-derived tumor xenograft(PDTX)and micro-metastatic abdominal ovarian cancer lesions were investigated.This novel strategy will establish a new method for early detection of ovarian cancer and significantly improve the prognosis of ovarian cancer patients.
基金supported by the National Key Research&Development Program of China(2020YFA0709900)the National Natural Science Foundation of China(22027805,22274024)+2 种基金the Major Project of Science and Technology of Fujian Province(2020HZ06006)the Young Elite Scientist Sponsorship Program by CAST(YESS20200110)China Postdoctoral Science Foundation(2022M720737,2021T140117)
文摘Gold nanoclusters(AuNCs)with near-infraredⅡ(NIR-Ⅱ)photoluminescence(PL)have emerged as novel bioimaging probes for in vivo disease diagnosis.So far,it still lacks a systematic review focusing on the synthesis,PL tuning,and in vivo imaging of NIR-Ⅱluminescent AuNCs.In this review,we briefly introduce the synthesis of NIR-Ⅱluminescent AuNCs using various surface ligands.We discuss the origins and properties of NIR-ⅡPL in AuNCs,and summarize the strategies for improving and/or tuning NIR-ⅡPL emissions.We also provide an overview of the recent progress in the application of AuNCs in tumor-targeted imaging,molecular imaging,and other areas(such as the sensitive imaging of bones,vessels,lymph nodes,etc.).Finally,we present the prospects and challenges in the field of NIR-Ⅱluminescent AuNCs and related imaging applications,expecting to offer comprehensive understanding of this field,and thereby deepening and broadening the biological application of AuNCs.
基金supported by National Natural Science Foundation of China(22022404,22074050,21904103)the Fundamental Research Funds for the Central Universities(CCNU22QN007)+4 种基金the Open Research Fund of State Key Laboratory of Chemo/Biosensing and Chemometrics(2021005)the Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules Hubei University(KLSAOFM2111)the Opening Project of Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education,Jianghan University(JDGD-202209)the Open Project Program of Key Laboratory for Analytical Science of Food Safety and Biology,Ministry of Education(FS2202)supported by the CRI project of National Research Foundation of Republic of Korea(2018R1A3B1052702,JSK)。
文摘Although metal-based chemical agents have demonstrated promising bacteriostatic effects in phototherapy,their short excitation/emission wavelengths and inadequate phototherapy efficiencies make their application in vivo difficult.We therefore synthesized a novel Pt(Ⅱ)metallacycle(Pt1110)that can be activated with a 980 nm laser for photodiagnosis/treatment in deep tissue.We found that Pt1110 significantly improved photothermal conversion(95%improvement)and ^(1)O_(2) generation(ΦΔ75%increase)compared to the ligand itself 1 and was well capable of light-induced sterilization under safe laser irradiation(0.72 W/cm^(2)).In addition,Pt1110 has little to no toxicity to cells.After incorporated into liposome,Pt1110 NPs was effective in wound healing in infection and keratitis models upon laser irradiation,which was accurately observed by NIR-Ⅱfluorescence imaging.This novel metal-coordinated supramolecular material has a potential to become a universal platform for phototherapy in deep tissue.
基金funded by the National Natural Science Foundation of China(Nos.22278038,21878023),the Program for Distinguished Professor of Liaoning Province.
文摘Detection of mercury ions(Hg^(2+))in actual samples is of significant importance due to the toxicity of Hg^(2+)to human health.In this work,a simple tetraphenylethene(TPE)derived fluorescent probe TPE-Hg based on aggregation-induced emission(AIE)mechanism was synthesized.TPE-Hg can visually recognize Hg^(2+)in THF/HEPES(1:9,v/v,HEPES 20 mmol/L,pH 7.3)system with rapid response,strong anti-interference ability,large Stokes shift(203 nm),and low detection limit(7.548×10^(-7)mol/L).The results show that Hg^(2+)triggered elimination of TPE-Hg lead to releasing of an AIE-active compound 2 is responsible to the sensing mechanism.TPE-Hg is applicable to detect Hg^(2+)in actual water samples and image Hg^(2+)in living MCF-7 cells.In addition,TPE-Hg is suitable to assay the Hg^(2+)level in seafood and tea samples,and it is alsoapplicable intest strips.
基金supported by the National Key R&D Program of China(2019YFA0210100)the National Natural Science Foundation of China(22234003,U21A20287,22374045,and21890744)。
