As a case of space-time interaction,near-repeat calculation indicates that when an event takes place at a certain location,its immediate geographical surroundings would face an increased risk of experiencing subsequen...As a case of space-time interaction,near-repeat calculation indicates that when an event takes place at a certain location,its immediate geographical surroundings would face an increased risk of experiencing subsequent events within a fairly short period of time.This paper presents an exploratory study that extends the investigation of the near-repeat phenomena to a series of space-time interaction,namely event chain calculation.Existing near-repeat tools can only deal with a limited amount of data due to computation constraints,let alone the event chain analysis.By deploying the modern accelerator technology and hybrid computer systems,this study demonstrates that large-scale near-repeat calculation or event chain analysis can be partially resolved through high-performance computing solutions to advance such a challenging statistical problem in both spatial analysis and crime geography.展开更多
Genetic analysis showed that cold tolerance at booting stage of near-isogenic lines （NILs） of Kunmingxiaobaigu was controlled by a gene with large phenotypic variance. One hundred and sixty-four simple sequence repe...Genetic analysis showed that cold tolerance at booting stage of near-isogenic lines （NILs） of Kunmingxiaobaigu was controlled by a gene with large phenotypic variance. One hundred and sixty-four simple sequence repeats （SSR） distributed over 12 chromosomes were used to screen polymorphism between Towata （recurrent parent, RP） and near-isogenic line pool （NILP）, and two SSR markers at the long arm of chromosome 5 showed polymorphism in comparison with RP genome. Of the two markers, RM31 was found possibly linked with the cold tolerance gene at booting stage through one-way ANOVA. Twelve SSR markers around RM31 were then used to detect polymorphism between RP and NIL, and only RM7452 had polymorphism. The gene of cold tolerance at booting stage was further mapped on chromosome 5 between RM7452 and RM31 with genetic distances of 4.8 cM and 8.0 cM, respectively. This gene explained 10.50% of phenotypic variance and 5.10% of phenotypic variance of fully filled grains, and was tentatively designated as Ctb（t）.展开更多
This paper studied the expert system of genotype discrimination for the STR locus D5S818 based on near-infrared spectroscopy-principal discriminant variate (PDV).Six genotypes,i.e.genotypes 10-10,10-11,11-11,11-12,11-...This paper studied the expert system of genotype discrimination for the STR locus D5S818 based on near-infrared spectroscopy-principal discriminant variate (PDV).Six genotypes,i.e.genotypes 10-10,10-11,11-11,11-12,11-13 and 13-13,were selected as research subjects.Based on the optimum polymerase chain reaction (PCR) conditions,about 54 measuring samples for each genotype were obtained;these samples were tested by near-infrared spectroscopy directly.With differences between homozygote genotypes and heterozygote ones,and differences of the total number of core repeat units between the six genotypes,two types of genotyping-tree structure were constructed and their respective PDV models were studied using the near-infrared spectra of the samples as recognition variables.Finally,based on the classification ability of these two genotyping-tree structures,an optimum expert system of genotype discrimination was built using the PDV models.The result demonstrated that the built expert system had good discriminability and robustness;without any preprocessing for PCR products,the six genotypes studied could be discriminated rapidly and correctly.It provided a methodological support for establishing an expert system of genotype discrimination for all genotypes of locus D5S818 and other STR loci.展开更多
在广谱抗稻瘟病品种云引与普感稻瘟病品种丽江新团黑谷(Lijiangxintuanheigu,LTH)构建的近等基因系抗、感病子代基因组重测序的差异基因中,从与免疫应答(immune response)相关的分类单元里得到8个基因序列有差异的基因.它们都为编码富...在广谱抗稻瘟病品种云引与普感稻瘟病品种丽江新团黑谷(Lijiangxintuanheigu,LTH)构建的近等基因系抗、感病子代基因组重测序的差异基因中,从与免疫应答(immune response)相关的分类单元里得到8个基因序列有差异的基因.它们都为编码富含亮氨酸重复序列的类受体蛋白激酶基因,并聚集于11号染色体的邻近位置,且与已知的白叶枯病抗性基因Xa21具有一定同源性.为进一步探究这些类受体蛋白激酶类基因在稻瘟病抗性中可能发挥的功能,我们分析了这些基因的结构、进化及抗感子代间的序列差异类型,同时还分别采用离体和喷雾接菌的方式对云引和LTH进行稻瘟病接种并取样.通过实时荧光定量PCR(quantitative real time polymerase chain reaction,q RT-PCR)发现,部分基因能受到稻瘟病菌的诱导,推测它们可能在水稻对抗稻瘟病病菌中发挥一定作用,为进一步挖掘稻瘟病相关抗性基因奠定基础.展开更多
文摘As a case of space-time interaction,near-repeat calculation indicates that when an event takes place at a certain location,its immediate geographical surroundings would face an increased risk of experiencing subsequent events within a fairly short period of time.This paper presents an exploratory study that extends the investigation of the near-repeat phenomena to a series of space-time interaction,namely event chain calculation.Existing near-repeat tools can only deal with a limited amount of data due to computation constraints,let alone the event chain analysis.By deploying the modern accelerator technology and hybrid computer systems,this study demonstrates that large-scale near-repeat calculation or event chain analysis can be partially resolved through high-performance computing solutions to advance such a challenging statistical problem in both spatial analysis and crime geography.
基金supportedby China National Natural Science Foundation(30160043,30260060) the Natural ScienceFoundation of Yunnan Province(2004CO010Z).
文摘Genetic analysis showed that cold tolerance at booting stage of near-isogenic lines （NILs） of Kunmingxiaobaigu was controlled by a gene with large phenotypic variance. One hundred and sixty-four simple sequence repeats （SSR） distributed over 12 chromosomes were used to screen polymorphism between Towata （recurrent parent, RP） and near-isogenic line pool （NILP）, and two SSR markers at the long arm of chromosome 5 showed polymorphism in comparison with RP genome. Of the two markers, RM31 was found possibly linked with the cold tolerance gene at booting stage through one-way ANOVA. Twelve SSR markers around RM31 were then used to detect polymorphism between RP and NIL, and only RM7452 had polymorphism. The gene of cold tolerance at booting stage was further mapped on chromosome 5 between RM7452 and RM31 with genetic distances of 4.8 cM and 8.0 cM, respectively. This gene explained 10.50% of phenotypic variance and 5.10% of phenotypic variance of fully filled grains, and was tentatively designated as Ctb（t）.
基金supported by grants from the National Natural Science Foundation of China (Grant no. 81001686)
文摘This paper studied the expert system of genotype discrimination for the STR locus D5S818 based on near-infrared spectroscopy-principal discriminant variate (PDV).Six genotypes,i.e.genotypes 10-10,10-11,11-11,11-12,11-13 and 13-13,were selected as research subjects.Based on the optimum polymerase chain reaction (PCR) conditions,about 54 measuring samples for each genotype were obtained;these samples were tested by near-infrared spectroscopy directly.With differences between homozygote genotypes and heterozygote ones,and differences of the total number of core repeat units between the six genotypes,two types of genotyping-tree structure were constructed and their respective PDV models were studied using the near-infrared spectra of the samples as recognition variables.Finally,based on the classification ability of these two genotyping-tree structures,an optimum expert system of genotype discrimination was built using the PDV models.The result demonstrated that the built expert system had good discriminability and robustness;without any preprocessing for PCR products,the six genotypes studied could be discriminated rapidly and correctly.It provided a methodological support for establishing an expert system of genotype discrimination for all genotypes of locus D5S818 and other STR loci.
文摘在广谱抗稻瘟病品种云引与普感稻瘟病品种丽江新团黑谷(Lijiangxintuanheigu,LTH)构建的近等基因系抗、感病子代基因组重测序的差异基因中,从与免疫应答(immune response)相关的分类单元里得到8个基因序列有差异的基因.它们都为编码富含亮氨酸重复序列的类受体蛋白激酶基因,并聚集于11号染色体的邻近位置,且与已知的白叶枯病抗性基因Xa21具有一定同源性.为进一步探究这些类受体蛋白激酶类基因在稻瘟病抗性中可能发挥的功能,我们分析了这些基因的结构、进化及抗感子代间的序列差异类型,同时还分别采用离体和喷雾接菌的方式对云引和LTH进行稻瘟病接种并取样.通过实时荧光定量PCR(quantitative real time polymerase chain reaction,q RT-PCR)发现,部分基因能受到稻瘟病菌的诱导,推测它们可能在水稻对抗稻瘟病病菌中发挥一定作用,为进一步挖掘稻瘟病相关抗性基因奠定基础.
