Effect of electroacupuncture at Jiaji (EX-B2) points on the expression of microRNA-21 and neuronal apoptosis in rats with acute spinal cord injury

Objective:To observe the effect of electroacupuncture at Jiaji (EX-B2) points on the expression of microRNA-21 and neuronal apoptosis in rats with acute spinal cord injury. Methods:SD rats were randomly divided into sham-operated group, model group, electro-acupuncture group and methylprednisolone group. The model of acute spinal cord injury was reproduced by modified Allen's method. Each group was given corresponding intervention treatment 2 hours after modeling. The electroacupuncture group was treated with electro-acupuncture at Jiaji (EX-B2) points in T8 to T12, the methylprednisolone group was given intraperitoneal injection of 30 mg/kg methylprednisolone sodium succinate, and the sham operation group and model group were not treated. After treatment, BBB scores were observed to evaluate the motor function of posterior limb in each group, and the injured spinal cord tissues were taken. The pathological changes of spinal cord neuropathy were observed by Nissl staining. The expression of microRNA-21 was detected by RT-qPCR, and the expression of apoptotic proteins (Bax, Bcl-2 and cleaved-Caspase-3) was detected by Western-Blot. Result:Compared with sham operation group, BBB, neuron survival rate and expression of microRNA-21 were significantly lower, apoptotic rate of neurons was significantly higher, expression of Bax, Bcl-2, cleaved Caspase-3 protein and Bax/Bcl-2 ratio were significantly increased in model group (P< 0.05). Electroacupuncture could significantly increase BBB score, neuron survival rate and expression of microRNA-21, reduce apoptotic rate of neurons and the ratio of Bax/Bcl-2, and inhibit the expression of Bax, Bcl-2 and cleaved Caspase-3 protein in tissues, which were significantly different from those of model group (P< 0.05). Conclusions: Electroacupuncture at Jiaji (EX-B2) points can significantly promote the recovery of neurological function in rats after ASCI, and its mechanism may be closely related to the up-regulation of the expression of microRNA-21 in tissues and the inhibition of the activation of Bax/Bcl-2/cleaved Caspase-3 signaling pathway.

THE TREATMENT OF CERVICAL SPONDYLOPATHY BY LASER ACUPUNCTURE AT THE CERVICAL JIAJI POINTS: AN ANALYSIS OF THERAPEUTIC EFFECT OF 102 CASES

Laser acupuncture therapy was employed in the treatment of spondylopathy.Thetherapeutic effect of the three types of the spondylopathy,nerve root type,vertebral artery type andspinal cord type were stuied and compared.The results indicate a significant difference between thethree types of spondylopathy.The best therapeutic effect was obtained in nerve root type with a remarkable effective rate of 98.5％;the effective rates for the vertebral artery type and the spinal cordtype were 92.3％and 25％,respectively.Combining laser irradiatlon with acupuncture,the therapyposseses the functions of acupuncture,heat effect and mild moxibustion action.Therefore,it showedeffect quickly and had the advantages of short treatment course and high therapeutic effect.Clinicalobservation has confirmed that laser acupuncture is more effective than single laser irradiation.Thistherapy offers another simple but effective method for the treatment of spondylopathy.

Effectiveness of electroacupuncture at Jiaji acupoints(EX-B 2), plus moxibustion and intermediate on postherpetic neuralgia: a randomized controlled trial

OBJECTIVE:To investigate the effectiveness of electroacupuncture at Jiaji acupoints(EX-B 2)plus moxibustion and intermediate frequency on postherpetic neuralgia(PHN).METHODS:A total of 140 outpatients who satisfied the inclusion criteria and volunteered for this treatment were randomly divided into treatment(n=70)and control(n=70)groups.Both groups received a localized lesion area and electroacupuncture treatment combined with moxibustion and intermediate frequency.The treatment group(TG)increased acupuncture at Jiaji acupoints(EX-B 2)and electroacupuncture.Pain and anxiety were assessed before and after 5,10,15,and 20 treatments by using visual pain simulation score(VAS)and Hamilton anxiety scale(HAMA),respectively.Clinical efficacy was also evaluated.RESULTS:The baseline between the two groups did not significantly differ(P>0.05).The VAS and HAMA scores of the two groups after treatment significantly decreased compared with those of various treatment stages(P>0.05).The HAMA score(P<0.01)of TG was lower than that of the control group(CG).The VAS score of TG was lower than that of CG in the 5th and 10th treatments(P<0.01).In the 15 th and 10 th scores,CG was also superior to TG(P<0.05).CONCLUSION:The combined treatment of electroacupuncture at Jiaji acupoints(EX-B 2),moxibustion,and intermediate frequency can relieve the pain and anxiety symptoms of PHN.The efficacy of the combined treatment was superior to traditional acupuncture.

Effects of electroacupuncture at “Jiaji ” (EX-B 2) on myocardial apoptosis and the expression of Bcl-2 and Bax in rats with ischemia-reperfusion injury

Objective To explore the protective mechanism of acupuncture on myocardial ischemia-reperfusion injury.Methods Fifty Wistar rats were randomly divided into a sham-operation group,a model group,a Jiaji group,a Neiguan group and a Yanglingquan group,10-rats

针刺头部穴配合夹脊治疗慢性疲劳综合征疗效观察

目的观察针刺头部穴配合夹脊治疗慢性疲劳综合征的临床疗效。方法将70例慢性疲劳综合征患者随机分为治疗组和对照组,每组35例。治疗组采用针刺头部穴配合夹脊治疗,对照组采用常规针刺治疗。观察两组治疗前后疲劳量表-14评分,并比较两组临床疗效。结果两组治疗后疲劳量表-14评分与同组治疗前比较,差异均具有统计学意义(P<0.01)。治疗组治疗后疲劳量表-14评分与对照组比较,差异具有统计学意义(P<0.05)。治疗组总有效率和愈显率分别为91.4%和71.4%,对照组分别为82.9%和45.7%。两组愈显率比较,差异具有统计学意义(P<0.05)。结论针刺头部穴配合夹脊是一种治疗慢性疲劳综合征的有效方法。

电针夹脊穴配合透灸法治疗颈型颈椎病35例

目的:观察电针夹脊穴配合透灸法治疗颈椎病的临床疗效。方法:将70例患者随机分为对照组和治疗组,每组各35例。治疗组采用电针夹脊穴配合透灸法治疗;对照组采用传统针灸治疗。观察WAS值的变化、治疗疗程、止痛时间、临床疗效、复发率等。结果:对照组有效率为85.7%,治疗组有效率为97.1%,两组有效率比较,差异具有统计学意义(P<0.05);治疗组治疗后VAS评分、止痛时间与对照组比较,差异具有统计学意义(P<0.05)。结论:电针夹脊穴配合透灸法治疗颈型颈椎病,操作方法简单、疗程短、疗效显著。

麦粒灸加针刺颈夹脊穴治疗儿童抽动症临床观察

目的观察麦粒灸加针刺颈夹脊穴治疗儿童抽动症的临床效果。方法将我院门诊就诊的46例儿童抽动症患儿随机分成治疗组和对照组,治疗组进行针刺第2、3、4、5颈夹脊穴加麦粒灸治疗,1次/日,10次为一个疗程;对照组进行托吡酯合硫必利药物治疗,观察治疗1个月后2组患儿的治疗效果和不良反应。结果治疗组患儿眨眼、皱额、肌肉抽动等明显减少,治疗有效率为95.65%,疗效优于对照组的51.17%(P<0.05),且无不良作用。结论麦粒灸加针刺颈夹脊穴可有效改善儿童抽动症的症状,显效快且安全有效,值得临床推广。

四位一体推拿疗法联合温针灸治疗神经根型颈椎病气滞血瘀证34例

目的:观察四位一体推拿疗法联合温针灸治疗神经根型颈椎病气滞血瘀证的临床疗效。方法:选取2017年10月至2019年10月本院诊治的神经根型颈椎病气滞血瘀证患者136例,按照随机数字表法分为对照组、推拿组、温针灸组和治疗组,每组34例。对照组给予颈复康冲剂内服治疗,推拿组在对照组治疗的基础上给予四位一体推拿疗法治疗,温针灸组在对照组治疗的基础上给予颈夹脊穴温针灸治疗,治疗组在对照组治疗的基础上给予四位一体推拿疗法联合温针灸治疗。观察四组患者临床疗效、复发率及治疗前后症状积分、P物质(substance P,SP)和白细胞介素-6 (interleukin-6,IL-6)水平、颈椎功能障碍指数(neck disability index, NDI)评分变化情况。结果:治疗组有效率88.23%,温针灸组有效率为61.76%,推拿组有效率为58.82%,对照组有效率为44.11%,治疗组有效率高于其他三组,差异有统计学意义(P<0.05);治疗组治疗后症状积分低于其他三组,差异具有统计学意义(P<0.05);治疗组治疗后SP、IL-6水平显著下降,且低于其他三组,差异具有统计学意义(P<0.05);治疗组治疗后NDI评分低于其他三组,差异具有统计学意义(P<0.05);治疗后6个月,治疗组复发率低于其他三组,但差异无统计学意义(P>0.05)。结论:四位一体推拿疗法联合温针灸治疗神经根型颈椎病气滞血瘀证,可显著缓解患者疼痛症状,亦可改善肌肉供血供氧情况及颈部生理功能,作用机制可能与调控血清SP、IL-6浓度,抑制炎性反应有一定相关性。

火针围刺联合夹脊电针治疗急性期带状疱疹:随机对照试验

ObjectivesTo investigate the clinical efficacy of surrounding fire needling combined with electroacupuncture (EA) at Jiājĭ (夹脊EX-B2) on acute herpes zoster, and explore the potential mechanism of this combined treatment by measuring the changes of serum inflammatory cytokines and pain mediators.MethodsSixty patients with acute herpes zoster were randomly assigned to either acupuncture plus western medicine group or western medicine group. In the western medicine group, the patients took orally mecobalamin and ganciclovir for 14 consecutive days. In the acupuncture plus western medicine group, on the base of the western medicine, the surrounding fire needling (fire needling was operated swiftly toward the center of skin lesions.) combined with EA at EX-B2 was provided. Acupoints of EX-B2 were selected based on the nerve ganglion corresponding to the skin lesions, as well as two adjacent nerve ganglions(one level upwards and one downwards) to the lesions, and were stimulated with EA once daily for 14 days. The change of symptoms from the blister eruption to scabbing and decrustation were observed. The score of visual analogue scale (VAS), serum inflammatory cytokines (interleukin-1β, interleukin-6, tumor necrosis factor-α) and pain mediators (calcitonin gene related peptide, substance P) before and after treatment, the scores of Pittsburgh sleep quality index (PSQI) and Hamilton depression scale (HAMD) before and after treatment as well as at 1 month of follow-up, and the overall effective rate and the incidence of post-hepatic neuralgia at 1 month of follow-up were assessed and compared between two groups, respectively.ResultsAfter treatment completion, the time of blister relief, scabbing and decrustation in the acupuncture plus western medicine group was significantly shorter when compared with that of the western medicine group (all P < 0.05). After treatment, VAS score, PSQI score and HAMD score were all reduced significantly when compared with that before treatment within group (P < 0.05). These scores in the acupuncture plus western medicine were reduced significantly compared with the western medicine group at the same time point (all P < 0.05). After treatment, the incidence of post-hepatic neuralgia was 6.89%, lower than that in western medicine group (50.00%, P < 0.05). After treatment, the values of serum inflammatory cytokines (IL-1β, IL-6, TNT-a) and pain mediators (CGRP, SP) were reduced when compared with the those before treatment within group (P < 0.05), and the reduction in the acupuncture plus western medicine group was larger than that in the western medicine group (P < 0.05). The total effective rate was 96.67% in the acupuncture plus western medicine group, higher than western medicine group (86.67%, P < 0.05).ConclusionsSurrounding fire needling therapy combined with EA at EX-B2 on the base of western medicine obtained better therapeutic effect on acute herpes zoster when compared with western medicine. This combined treatment may effectively relieve pain and depressive emotions, improve sleep quality and reduce the incidence of post-hepatic neuralgia. The underlying mechanism may related to regulation of pain mediators and reduction of inflammatory cytokines.

针刺颈夹脊穴联合推拿对主观认知下降患者认知功能的影响

目的:在生活方式健康宣教、元记忆训练基础上,观察针刺颈夹脊穴联合推拿对主观认知下降(SCD)患者认知功能、睡眠质量、血流动力学指标的影响。方法:将60例SCD患者随机分为观察组(30例,脱落1例)和对照组(30例,脱落3例)。对照组予生活方式健康宣教和元记忆训练,每天训练2次,每次15 min,每周5 d,连续4周;在对照组基础上,观察组采用针刺颈夹脊穴联合推拿治疗,先使用一指禅偏锋推法结合拨法分别沿足太阳膀胱经和督脉按摩颈部10~15 min,然后针刺双侧C1-C7颈夹脊穴,留针30 min,每日1次,每周5次,连续4周。于治疗前后,观察两组简易智能精神状态量表(MMSE)评分、韦克斯勒记忆量表第四版(WMS-Ⅳ)评估的总记忆商(FSMQ)评分、匹兹堡睡眠质量指数(PSQI)评分,采用经颅多普勒超声检测两组患者血流动力学指标[左侧椎动脉(LVA)、右侧椎动脉(RVA)、基底动脉(BA)的平均血流速度(Vm)和血管搏动指数(PI)]。结果:治疗后,两组患者MMSE、FSMQ评分较治疗前升高(P<0.05,P<0.001),观察组PSQI评分较治疗前降低(P<0.05),观察组BA的Vm值较治疗前升高(P<0.001);观察组MMSE、FSMQ评分以及BA的Vm值均高于对照组(P<0.05,P<0.001),PSQI评分低于对照组(P<0.05)。结论:针刺颈夹脊穴联合推拿在改善SCD患者认知功能、缓解慢性情绪应激和改善睡眠状态方面更具优势,其作用机制可能是改善BA的供血。

Treatment of Herpes Zoster with Electroacupuncture plus Collateral-pricking and Cupping Therapy

Objective:To observe the clinical effect of electroacupuncture(EA)plus collateral-pricking and cupping therapy for herpes zoster.Methods:Fifty-three cases with herpes zoster were randomly divided into two groups.Thirty-one cases in the treatment group were treated with EA at Jiaji(EX-B 2)points plus local collateral-pricking and cupping therapy.Twenty-two cases in the control group were treated with oral administration of Valaciclovir Hydrochloride and Indomethacin.Results:The curative and remarkable effective rate was 93.5%in the treatment group and 72.7%in the control group.The difference between the two groups was statistically significant(P<0.01),indicating that EA plus collateral-pricking and cupping therapy is better than Western medications in the clinical effects for herpes zoster.Conclusion:The EA plus collateral-pricking and cupping method is an effective therapy for herpes zoster.

针刺“手四针”治疗风寒湿型颈型颈椎病临床观察

目的:比较"手四针"与常规针刺颈夹脊治疗风寒湿型颈型颈椎病的临床疗效差异。方法:将80例颈型颈椎病患者随机分为观察组和对照组,每组40例。观察组取患侧三间、外劳宫、中渚、后溪四穴,对照组取双侧颈4、颈5、颈6夹脊穴,均隔日针刺1次,分别于治疗前、首次治疗后即刻、治疗半个月后、治疗1个月后观察两组患者疼痛视觉模拟评分(VAS)及治疗1个月后的临床疗效。结果:两组治疗后各观察时点疼痛VAS评分均较治疗前降低(均P<0.05),观察组治疗后即刻的疼痛VAS评分较对照组降低更明显(P<0.01);观察组和对照组的治愈率分别为52.5%(21/40)、32.5%(13/40),总有效率分别为95.0%(38/40)、82.5%(33/40),观察组疗效优于对照组(均P<0.05)。结论:"手四针"与常规针刺颈夹脊治疗方法对于风寒湿型颈型颈椎病均有很好的疗效,"手四针"疗法的即刻疗效更显著。

A Clinical Observation of Functional Abdominal Pain Syndrome in Patients Treated by Traditional Chinese Spinal Orthopedic Manipulation

Objective： To evaluate the clinical effect of traditional Chinese spinal orthopedic manipulation（TCSOM） in treating patients with functional abdominal pain syndrome（FAPS） in comparison with Pinaverium Bromide（Dicetel, PBD）, and to assess a possible cause for FAPS. Methods： Eighty patients with FAPS were randomly and equally assigned to the TCSOM group and PBD group according to the random number table. All patients in the TCSOM group were treated with a maximum of 5 times of spinal manipulations. Patients in the PBD group were instructed to take 50 mg 3 times a day, consistently for 2 weeks. The symptoms of pre-and post-treatment were assessed on a visual analog scale（VAS） pain score. A symptom improvement rating（SIR） was implemented to evaluate the effects of the treatments. Results： The symptoms of 27 cases of the TCSOM group were relieved soon after the first TCSOM treatment and 9 cases were significantly improved. The VAS pain scores in the TCSOM group were significantly lower than those in the PBD group after 2 weeks treatment. According to the SIR based on VAS, the TCSOM group included 30 cases with excellent results, 7 cases with good, and 3 cases with poor. Adverse events to the treatment were not reported. Based on VAS, the PBD group reported 8 cases with excellent results, 10 cases with good and 22 cases with poor. There was a significant difference between the two groups（P〈0.01）. Conclusions： The displacement of intervertebral discs and/or vertebra in the thoracic or lumbar region seems to be a contributing factor in the symptoms of FAPS. TCSOM is an effective treatment for FAPS.

项丛刺结合颈夹脊穴针刺、TDP神灯照射治疗颈源性眩晕50例临床观察

目的观察项丛刺结合颈夹脊穴针刺、TDP神灯照射治疗颈源性眩晕的临床疗效。方法将97例颈源性眩晕患者随机分为治疗组和对照组。对照组47例采用颈夹脊穴针刺联合TDP神灯照射治疗,治疗组50例在对照组治疗方法的基础上结合项丛刺治疗。连续治疗10次为1个疗程,2个疗程后统计疗效。结果治疗组总有效率为94.00%,显著高于对照组的80.85%,2组比较差异有统计学意义(P<0.05);与同组治疗前比较,2组治疗后颈性眩晕症状与功能评估(ESCV)评分显著升高,差异均有统计学意义(P<0.05),且治疗组治疗后ESCV评分显著高于对照组,差异有统计学意义(P<0.01)。结论项丛刺结合颈夹脊穴针刺、TDP神灯照射治疗颈源性眩晕疗效满意,并能迅速缓解眩晕、头痛、颈肩痛等症状,值得临床推广运用。