To accelerate the breeding and selection of Pinus densiflora Siebold and Zucc. resistance to pine wilt disease, a micropropagation system was established and nematode resistance evaluated in vitro. Cotyledon-hypocotyl...To accelerate the breeding and selection of Pinus densiflora Siebold and Zucc. resistance to pine wilt disease, a micropropagation system was established and nematode resistance evaluated in vitro. Cotyledon-hypocotyl explants from 28-day-old seedlings were first cultured on Gresshoff and Doy medium supplemented with 4.0 mg L^(-1) 6-benzyladenine and 0.2 mg L^(-1) a-naphthaleneacetic acid(NAA) to stimulate the formation of buds. Induced buds were subsequently subcultured on Gupta and Durzan medium supplemented with 0.1%(w/v)activated charcoal for elongation. Stem sections derived from shoots were used as explants for the further multiplication. Roots were formed from shoots transferred to woody plant medium containing 0.2 mg L^(-1) NAA for4 weeks. The nematode resistance test showed that symptoms in micropropagated shoots after infection with pine wood nematode(PWN) were similar to those in plants infected in the field. The wilting rate varied from 20 to100% among different clones 18 days after inoculation.The most susceptible clone was Clone 6-4 with a 100%wilting rate, while Clone 8-4 showed a relatively high resistance with a 20% wilting rate. The number of nematodes recovered from Clone 8-4 shoots was significantly lower(P = 0.05) than from Clones 5-10 and 16-4. This work contributes to the breeding of PWN resistance in P.densiflora.展开更多
Pinus densiflora needle extract (PDNE) is widely reported to have many pharmacological activities including antioxidant potential. However, the solvent system used for extraction greatly affects its antioxidant qualit...Pinus densiflora needle extract (PDNE) is widely reported to have many pharmacological activities including antioxidant potential. However, the solvent system used for extraction greatly affects its antioxidant quality. Hence, in the present study, we investigated the effect of a different ratio (vol/vol) of ethanol to water (0-100%) in the extraction of PDNE with potent antioxidant capacity. The chemical assays, 2,2-diphenyl-1 picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), were conducted to assess the antioxidant potential of PDNE. Subsequently, the cytoprotective effect of PDNE was determined using tert-butyl hydroperoxide (TBHP)-challenged HepG2 cellular model. The needle extracts from 40% ethanol (PDNE-40) showed greater radical scavenging activity followed by 60%, 20%, 80%, 0% and 100% ethanol extracts. EC50 value of the most active extract, PDNE-40, was 8.56 ± 0.51 μg/mL, relative to 1.34 ± 0.28 μg/mL of the standard trolox (for ABTS radical), and 75.96 ± 11.60 μg/mL, relative to 4.83 ± 0.26 μg/mL of the standard trolox (for DPPH radical). Either PDNE-20 or PDNE-40 pretreatment remarkably decreased the levels of reactive oxygen species (ROS), lipid peroxides and protein carbonyls in TBHP-challenged HepG2 cells. In addition, both PDNE-20 and PDNE-40 significantly reversed the decreased ratio of reduced (GSH) to oxidized (GSSG) glutathione. Moreover, these two extracts showed a significant inhibitory effect on TBHP-induced nuclear damage and loss of cell viability. In summary, the inclusion of 40% ethanol in water for extraction of Pinus densiflora needle greatly increases the antioxidant quality of the extract.展开更多
We estimated the growth volume of artificially reforested Pinus densiflora in a post-fire area on three different contour conditions by comparing and analyzing the vertical and horizontal distributions of below-ground...We estimated the growth volume of artificially reforested Pinus densiflora in a post-fire area on three different contour conditions by comparing and analyzing the vertical and horizontal distributions of below-ground roots on each contour. The main roots at the south-facing slope (SS) developed in a long and straight form, and those on the north-facing slope (NS) in a twisted form. The side roots developed more than the main roots on the Ridge. The depth of taproots decreased in the following order: SS > NS > Ridge. The roots on the SS developed in a pile-form root structure whereas those in the Ridge and NS developed concentrically near the root collar. The amount of root development decreased in the following order: SS > NS > Ridge. The ratio of fine roots from the whole-root development decreased in the following order: Ridge > SS > NS. These results can guide considerations of growth differences according to the planting contour conditions for future establishment of P. densiflora artificial plantations.展开更多
We studied the impact of stand density and forest floor humus removal on regeneration ofPinus densiflora in a mixed co- nifer and broad-leaved forest on the Daimonji-Yama Mountain, Kyoto, Japan. Three levels of stand ...We studied the impact of stand density and forest floor humus removal on regeneration ofPinus densiflora in a mixed co- nifer and broad-leaved forest on the Daimonji-Yama Mountain, Kyoto, Japan. Three levels of stand density were set, i.e., a clear-cut site, a heavily thinned site and a lightly thinned site. In each density treatment, comparisons were carried out between humus removal （A0-free） and humus intact （A0-intact） subplots. We counted the emergence of sown pine seeds and measured survival and growth of pine seedlings over the next four years. In addition, light conditions and the recovery of understory were monitored. Results show that thinning intensity and humus removal promoted the establishment and growth of seedlings. One exception, however, was that seedling growth was minimal in the heavily thinned A0-intact subplots. This was due to a strong response of understory vegetation growth on the treatment combination, which hindered the pine seedling growth. Furthermore, we found that the canopy openness measured at 1.3-m height remained at least 35% for the heavily thinned site in 2008. The results suggest that the removal of the A0 layer after heavy thinning is the most effective and labor-saving operation for P. densifiora regeneration.展开更多
Litter decomposition and ecological stoichiometry of nutrient release is an important part of material cycling and energy flow in forest ecosystems.In a study of the ecological stoichiometry and nutrient release durin...Litter decomposition and ecological stoichiometry of nutrient release is an important part of material cycling and energy flow in forest ecosystems.In a study of the ecological stoichiometry and nutrient release during litter decomposition in a pine–oak forest ecosystem of the Grain to Green Program(GTGP)area of northern China,a typical pine and oak species(PDS:Pinus densiflora Sieb.,QAC:Quercus acutissima Carr.)were selected in the Taiyi Mountain study area.The ecological stoichiometry characteristics of carbon(C),nitrogen(N)and phosphorus(P)and litter decomposition dynamics were studied by field sampling and quantitative analyses.The results showed the following.(1)The decomposition dynamics of both litters was slow-fast-slow.The most important climatic factor affecting the litter decomposition rate from May to October was precipitation and temperature from November to April of the following year.(2)Throughout the 300-day study,in both litters,C of the two litters was released,N first accumulated and was then released,and P exhibited a release-accumulate-release pattern.(3)C:P was significantly higher than C:N and N:P(p<0.05);the C:N of PSD litter was higher than that of QAC(p<0.05),but the N:P of QAC litter was higher than that of PSD litter(p<0.05).The C:N of both litters was very high in the study area,indicating that the nutrient release ability during litter decomposition in the two typical pine–oak forest ecosystems was relatively weak;therefore,more attention should be paid to nitrogen-fixing species and mixed forests in the GTGP area of northern China.展开更多
This study was conducted on the analytic tree and got the fitting empirical equation of tree growth, in which the tree growth rate was used as the variable and time as the independent variable. The arithmetical operat...This study was conducted on the analytic tree and got the fitting empirical equation of tree growth, in which the tree growth rate was used as the variable and time as the independent variable. The arithmetical operation to the function got the mature age of tree growth, and the practical and ideal mature ages of Pinus densiflora were 58 and 123.5 a, respectively. In addition, the application research direction and matters needing attention were proposed.展开更多
We used preliminary data to estimate the growth volume of artificially reforested Pinus densiflora in a post-fire area on three different contour conditions. We compared the growth of P. densiflora on a south-facing s...We used preliminary data to estimate the growth volume of artificially reforested Pinus densiflora in a post-fire area on three different contour conditions. We compared the growth of P. densiflora on a south-facing slope(Ssth), north-facing slope(Snth) and ridge area(Ridge), using 7 trees selected from each stand aspect. The tree height, diameter and growth volume were measured and the dry weight of each plant part were compared and analyzed. The results revealed that the total dry weight was highest on Ssth(5992.3 g), followed by Snth(4833.2 g) and lowest on Ridge(3160.1 g). The height growth was highest on Snth(285.8 cm), followed by Ssth(274.5 cm) and lowest on Ridge(211.5 cm). The diameter growth was greatest on Ssth(7.37 cm), followed by Snth(7.10 cm) and lowest on Ridge(5.72 cm). The volume growth was highest on Ssth(4257.7 cm3), followed by Snth(3750.7 cm3) and lowest on Ridge(2093.7 cm3). Therefore, we should consider and include the concept of slope orientation together with differences in habitat environments in afforestation projects when creating artificial forests with P. densiflora. These study results can serve as important preliminary data for future establishment of artificial forest of P. densiflora in a post-fire plantation.展开更多
试验地点选在延吉市林业局的帽儿山林场、龙井市林业局的智新林场、勇新林场和延边大学农学院后马蹄山,共选伐样木28株,实测每株样木0.1、0.2、0.3、0.4、0.5、0.6、0.7、0.8、0.9 h的相对高处直径。计算各株样木的相对直径,并进行了对...试验地点选在延吉市林业局的帽儿山林场、龙井市林业局的智新林场、勇新林场和延边大学农学院后马蹄山,共选伐样木28株,实测每株样木0.1、0.2、0.3、0.4、0.5、0.6、0.7、0.8、0.9 h的相对高处直径。计算各株样木的相对直径,并进行了对比分析,结果为天然林赤松和人工林赤松的相对直径不因起源的不同而不同;进而对同一林分内不同赤松样木的干形进行了分析,结果为其相对干形基本一致。通过回归得到预测赤松单木任意部位直径经验方程为D n:=0.1315 D 1.3+1.2763n D 1.3+1.9645n-0.0265。在树干干形的测算理论中,干形一直是人们研究的热点问题。有关赤松相对干形的研究尚未见报道,为林业生产实践与科学的研究提供理论依据。展开更多
The carbon(C) and nitrogen(N) storage capabilities of Pinus densiflora in six different stand ages(10,27,30,32,44,and 71 years old) were investigated in Korea.Thirty sample trees were destructively harvested and 12 we...The carbon(C) and nitrogen(N) storage capabilities of Pinus densiflora in six different stand ages(10,27,30,32,44,and 71 years old) were investigated in Korea.Thirty sample trees were destructively harvested and 12 were excavated.Samples from the above and belowground tree components,coarse woody debris(CWD),forest floor,and mineral soil(0-30 cm) were collected.Tree biomass was highest in the 71-year-old stand(202.8 t ha-1) and lowest in the 10-year-old stand(18.4 t ha-1).C and N storage in the mineral soil was higher in the 71-year-old stand than in the other stands,mainly due to higher soil C and N concentrations.Consequently,the total ecosystem C and N storage(tree+forest floor+CWD+soil) was positively correlated with stand age:increasing from a minimum in the 10 year old stand(18.8 t C ha-1 and 1.3 t N ha-1) to a maximum in the 71-year-old stand(201.4 t C ha-1 and 8.5 t N ha-1).The total ecosystem C storage showed a similar sigmoidal pattern to that of tree C storage as a function of the age-sequence,while N storage in the CWD,forest floor and mineral soil showed no significant temporal trends.Our results provide important insights that will increase our understanding of C and N storage in P.densiflora stands and our ability to predict changes according to stand age in the region.展开更多
Pinus densiflora Sieb.et Zucc. forest is a zonal coniferous forest in Shandong Peninsula. The age structure, diameter at breast height(DBH) and height of natural P. densiflora population in mount Kunyu showed that the...Pinus densiflora Sieb.et Zucc. forest is a zonal coniferous forest in Shandong Peninsula. The age structure, diameter at breast height(DBH) and height of natural P. densiflora population in mount Kunyu showed that the population was in an increasing stage. The distributed characteristics of seed bank and seedlings also indicated P. densiflora is more adaptable to native environment. Moreover, the species diversity of shrub and herbaceous layer under natural P. densiflora forest was remarkably greater than that under cultivated P. thunbergii forest. To realize natural restoration and sustainable development of vegetation in Shandong Peninsula, we must conserve and develop P. densiflora forest.展开更多
基金funded by the Special Research Program for Forestry Sectors Beneficial to Public,State Forestry Administration,China(Grant No.201204501)the Science and Technology Support Program of Jiangsu Province(BE2014405)+1 种基金the Key University Science Research Project of Jiangsu Province(15KJA220003)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘To accelerate the breeding and selection of Pinus densiflora Siebold and Zucc. resistance to pine wilt disease, a micropropagation system was established and nematode resistance evaluated in vitro. Cotyledon-hypocotyl explants from 28-day-old seedlings were first cultured on Gresshoff and Doy medium supplemented with 4.0 mg L^(-1) 6-benzyladenine and 0.2 mg L^(-1) a-naphthaleneacetic acid(NAA) to stimulate the formation of buds. Induced buds were subsequently subcultured on Gupta and Durzan medium supplemented with 0.1%(w/v)activated charcoal for elongation. Stem sections derived from shoots were used as explants for the further multiplication. Roots were formed from shoots transferred to woody plant medium containing 0.2 mg L^(-1) NAA for4 weeks. The nematode resistance test showed that symptoms in micropropagated shoots after infection with pine wood nematode(PWN) were similar to those in plants infected in the field. The wilting rate varied from 20 to100% among different clones 18 days after inoculation.The most susceptible clone was Clone 6-4 with a 100%wilting rate, while Clone 8-4 showed a relatively high resistance with a 20% wilting rate. The number of nematodes recovered from Clone 8-4 shoots was significantly lower(P = 0.05) than from Clones 5-10 and 16-4. This work contributes to the breeding of PWN resistance in P.densiflora.
基金the Korea Forestry Promotion Institute (R&D program for Forest Science Technology, Grant No. 2013070B101819-AA03)Kookmin University, South Korea, (Grant No. 2017) for financial support
文摘Pinus densiflora needle extract (PDNE) is widely reported to have many pharmacological activities including antioxidant potential. However, the solvent system used for extraction greatly affects its antioxidant quality. Hence, in the present study, we investigated the effect of a different ratio (vol/vol) of ethanol to water (0-100%) in the extraction of PDNE with potent antioxidant capacity. The chemical assays, 2,2-diphenyl-1 picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), were conducted to assess the antioxidant potential of PDNE. Subsequently, the cytoprotective effect of PDNE was determined using tert-butyl hydroperoxide (TBHP)-challenged HepG2 cellular model. The needle extracts from 40% ethanol (PDNE-40) showed greater radical scavenging activity followed by 60%, 20%, 80%, 0% and 100% ethanol extracts. EC50 value of the most active extract, PDNE-40, was 8.56 ± 0.51 μg/mL, relative to 1.34 ± 0.28 μg/mL of the standard trolox (for ABTS radical), and 75.96 ± 11.60 μg/mL, relative to 4.83 ± 0.26 μg/mL of the standard trolox (for DPPH radical). Either PDNE-20 or PDNE-40 pretreatment remarkably decreased the levels of reactive oxygen species (ROS), lipid peroxides and protein carbonyls in TBHP-challenged HepG2 cells. In addition, both PDNE-20 and PDNE-40 significantly reversed the decreased ratio of reduced (GSH) to oxidized (GSSG) glutathione. Moreover, these two extracts showed a significant inhibitory effect on TBHP-induced nuclear damage and loss of cell viability. In summary, the inclusion of 40% ethanol in water for extraction of Pinus densiflora needle greatly increases the antioxidant quality of the extract.
基金supported by a research Grant from Yeungnam University in 2015the National Research Foundation of Korea Grant(NRF No.2016M3C1B6929073)funded by the Ministry of Science,ICT and Future Planning of the Republic of Korea
文摘We estimated the growth volume of artificially reforested Pinus densiflora in a post-fire area on three different contour conditions by comparing and analyzing the vertical and horizontal distributions of below-ground roots on each contour. The main roots at the south-facing slope (SS) developed in a long and straight form, and those on the north-facing slope (NS) in a twisted form. The side roots developed more than the main roots on the Ridge. The depth of taproots decreased in the following order: SS > NS > Ridge. The roots on the SS developed in a pile-form root structure whereas those in the Ridge and NS developed concentrically near the root collar. The amount of root development decreased in the following order: SS > NS > Ridge. The ratio of fine roots from the whole-root development decreased in the following order: Ridge > SS > NS. These results can guide considerations of growth differences according to the planting contour conditions for future establishment of P. densiflora artificial plantations.
基金supported by a Grant-in-Aid from the Ministry of Education,Science and Culture of Japan(No.17510025)
文摘We studied the impact of stand density and forest floor humus removal on regeneration ofPinus densiflora in a mixed co- nifer and broad-leaved forest on the Daimonji-Yama Mountain, Kyoto, Japan. Three levels of stand density were set, i.e., a clear-cut site, a heavily thinned site and a lightly thinned site. In each density treatment, comparisons were carried out between humus removal （A0-free） and humus intact （A0-intact） subplots. We counted the emergence of sown pine seeds and measured survival and growth of pine seedlings over the next four years. In addition, light conditions and the recovery of understory were monitored. Results show that thinning intensity and humus removal promoted the establishment and growth of seedlings. One exception, however, was that seedling growth was minimal in the heavily thinned A0-intact subplots. This was due to a strong response of understory vegetation growth on the treatment combination, which hindered the pine seedling growth. Furthermore, we found that the canopy openness measured at 1.3-m height remained at least 35% for the heavily thinned site in 2008. The results suggest that the removal of the A0 layer after heavy thinning is the most effective and labor-saving operation for P. densifiora regeneration.
基金The study was subsidized by Grants from the Natural Science Foundation of Shandong Province of China(No.ZR2016CM49)the Special Fund for Forestry Scientific Research in the Public Interest(No.201404303-08).This work was supported by CFERN and BEIJING TECHNO SOLUTIONS Award Funds for excellent academic achievements.
文摘Litter decomposition and ecological stoichiometry of nutrient release is an important part of material cycling and energy flow in forest ecosystems.In a study of the ecological stoichiometry and nutrient release during litter decomposition in a pine–oak forest ecosystem of the Grain to Green Program(GTGP)area of northern China,a typical pine and oak species(PDS:Pinus densiflora Sieb.,QAC:Quercus acutissima Carr.)were selected in the Taiyi Mountain study area.The ecological stoichiometry characteristics of carbon(C),nitrogen(N)and phosphorus(P)and litter decomposition dynamics were studied by field sampling and quantitative analyses.The results showed the following.(1)The decomposition dynamics of both litters was slow-fast-slow.The most important climatic factor affecting the litter decomposition rate from May to October was precipitation and temperature from November to April of the following year.(2)Throughout the 300-day study,in both litters,C of the two litters was released,N first accumulated and was then released,and P exhibited a release-accumulate-release pattern.(3)C:P was significantly higher than C:N and N:P(p<0.05);the C:N of PSD litter was higher than that of QAC(p<0.05),but the N:P of QAC litter was higher than that of PSD litter(p<0.05).The C:N of both litters was very high in the study area,indicating that the nutrient release ability during litter decomposition in the two typical pine–oak forest ecosystems was relatively weak;therefore,more attention should be paid to nitrogen-fixing species and mixed forests in the GTGP area of northern China.
文摘This study was conducted on the analytic tree and got the fitting empirical equation of tree growth, in which the tree growth rate was used as the variable and time as the independent variable. The arithmetical operation to the function got the mature age of tree growth, and the practical and ideal mature ages of Pinus densiflora were 58 and 123.5 a, respectively. In addition, the application research direction and matters needing attention were proposed.
基金supported by a research grant from Yeungnam University in 2015
文摘We used preliminary data to estimate the growth volume of artificially reforested Pinus densiflora in a post-fire area on three different contour conditions. We compared the growth of P. densiflora on a south-facing slope(Ssth), north-facing slope(Snth) and ridge area(Ridge), using 7 trees selected from each stand aspect. The tree height, diameter and growth volume were measured and the dry weight of each plant part were compared and analyzed. The results revealed that the total dry weight was highest on Ssth(5992.3 g), followed by Snth(4833.2 g) and lowest on Ridge(3160.1 g). The height growth was highest on Snth(285.8 cm), followed by Ssth(274.5 cm) and lowest on Ridge(211.5 cm). The diameter growth was greatest on Ssth(7.37 cm), followed by Snth(7.10 cm) and lowest on Ridge(5.72 cm). The volume growth was highest on Ssth(4257.7 cm3), followed by Snth(3750.7 cm3) and lowest on Ridge(2093.7 cm3). Therefore, we should consider and include the concept of slope orientation together with differences in habitat environments in afforestation projects when creating artificial forests with P. densiflora. These study results can serve as important preliminary data for future establishment of artificial forest of P. densiflora in a post-fire plantation.
文摘试验地点选在延吉市林业局的帽儿山林场、龙井市林业局的智新林场、勇新林场和延边大学农学院后马蹄山,共选伐样木28株,实测每株样木0.1、0.2、0.3、0.4、0.5、0.6、0.7、0.8、0.9 h的相对高处直径。计算各株样木的相对直径,并进行了对比分析,结果为天然林赤松和人工林赤松的相对直径不因起源的不同而不同;进而对同一林分内不同赤松样木的干形进行了分析,结果为其相对干形基本一致。通过回归得到预测赤松单木任意部位直径经验方程为D n:=0.1315 D 1.3+1.2763n D 1.3+1.9645n-0.0265。在树干干形的测算理论中,干形一直是人们研究的热点问题。有关赤松相对干形的研究尚未见报道,为林业生产实践与科学的研究提供理论依据。
基金supported by research grants from the Korea Forest Research Institute (Grant No. S110708L0101111W)National Research Foundation of Korea (Grant Nos. R01-2006-000-10863-0 and A307-K001)
文摘The carbon(C) and nitrogen(N) storage capabilities of Pinus densiflora in six different stand ages(10,27,30,32,44,and 71 years old) were investigated in Korea.Thirty sample trees were destructively harvested and 12 were excavated.Samples from the above and belowground tree components,coarse woody debris(CWD),forest floor,and mineral soil(0-30 cm) were collected.Tree biomass was highest in the 71-year-old stand(202.8 t ha-1) and lowest in the 10-year-old stand(18.4 t ha-1).C and N storage in the mineral soil was higher in the 71-year-old stand than in the other stands,mainly due to higher soil C and N concentrations.Consequently,the total ecosystem C and N storage(tree+forest floor+CWD+soil) was positively correlated with stand age:increasing from a minimum in the 10 year old stand(18.8 t C ha-1 and 1.3 t N ha-1) to a maximum in the 71-year-old stand(201.4 t C ha-1 and 8.5 t N ha-1).The total ecosystem C storage showed a similar sigmoidal pattern to that of tree C storage as a function of the age-sequence,while N storage in the CWD,forest floor and mineral soil showed no significant temporal trends.Our results provide important insights that will increase our understanding of C and N storage in P.densiflora stands and our ability to predict changes according to stand age in the region.
文摘Pinus densiflora Sieb.et Zucc. forest is a zonal coniferous forest in Shandong Peninsula. The age structure, diameter at breast height(DBH) and height of natural P. densiflora population in mount Kunyu showed that the population was in an increasing stage. The distributed characteristics of seed bank and seedlings also indicated P. densiflora is more adaptable to native environment. Moreover, the species diversity of shrub and herbaceous layer under natural P. densiflora forest was remarkably greater than that under cultivated P. thunbergii forest. To realize natural restoration and sustainable development of vegetation in Shandong Peninsula, we must conserve and develop P. densiflora forest.
