Protein extraction is a critical step for two-dimensional electrophoresis （2-DE）. Different plant samples require different and adaptive protein extraction protocols. The leaves of medicinal plant, Baphicacanthus cu...Protein extraction is a critical step for two-dimensional electrophoresis （2-DE）. Different plant samples require different and adaptive protein extraction protocols. The leaves of medicinal plant, Baphicacanthus cusia （Nees） Bremek are notoriously recalcitrant to common protein extraction methods due to high levels of interfering compounds （especially the secondary metabolites and pigments）. This study was aimed to establish a routine procedure for the proteomic analysis ofB. cusia leaves, and a new protocol for the protein extraction was developed by optimizing trichloroacetic acid （TCA）/ acetone extraction method. The efficiency of this protocol was demonstrated by comparison with 3 published protein extraction methods （chloroform/acetone, Mg/NP-40, Tris-base/acetone）. The results showed that the optimized TCA/ acetone precipitation extraction method gave a relatively high protein yield （9.263 mg g^-1 fresh weight）, high-resolution separation, clear protein profiles, the highest proteins spots （1 31 t protein spots）, and displayed less contamination in 2- DE gels. Therefore, the results suggested that the optimized TCA/acetone method was the most effective among the 4 methods for B. cusia leaves.展开更多
The aim of this experiment is to study the mechanism of APN in alleviating the Ca2+-overloading in dog model during the process of ischemic reperfusion. In comparison with the sustained ischemic group, the parameters ...The aim of this experiment is to study the mechanism of APN in alleviating the Ca2+-overloading in dog model during the process of ischemic reperfusion. In comparison with the sustained ischemic group, the parameters in the ischemic reperfusion group demonstrated: Ca2+ of ischemic region of myocardial cell increased (P<0.05) , Na+ increased remarkably(P<0. 01), the activity of Ca2+-ATPase dropped remarkably(P<0. 01),and MDA increased significantly(P<0. 01). Whereas in the group pretreated with APN, the Ca2+ in the relevant area reduced (P<0.05), Na+ decreased significantly(P<0. 01), the activity of Ca2+-ATPase and Na+-K+ ATPase increased remarkably(P<0.01) ,and MDA decreased significantly (P<0. 01). These findings indicate that APN may improve the activity of sarcolemma ATPase in alleviating the Ca2+ and Na+-overloading by decreasing the harmful effect of oxygen free radicals.展开更多
Machilus spp.are mostly valuable timber and economic tree species,widely used in many fields such as artificial afforestation,landscaping,furniture and building materials,chemicals and medicine.In this article,the dis...Machilus spp.are mostly valuable timber and economic tree species,widely used in many fields such as artificial afforestation,landscaping,furniture and building materials,chemicals and medicine.In this article,the distribution,uses and domestic resources of Machilus Nees are summarized.Focus is placed on the analysis of propagation methods,including seed propagation,cutting propagation and tissue culture propagation.The research status and existing problems are summarized,and the future research focus on the propagation technology of Machilus Nees is prospected,with a view to provide theoretical references for the efficient breeding of high-quality seedlings and comprehensive promotion and application of Machilus spp.and contribute to the conservation and sustainable use of wild resources.展开更多
[Objectives]To observe the effects of polysaccharides from Dicliptera chinensis(L.)Nees.on the expression of TLR/NF-κB pathway related proteins in HepG2 cells induced by oleic acid,and to explore the possible mechani...[Objectives]To observe the effects of polysaccharides from Dicliptera chinensis(L.)Nees.on the expression of TLR/NF-κB pathway related proteins in HepG2 cells induced by oleic acid,and to explore the possible mechanism of polysaccharides from D.chinensis(L.)Nees.in the treatment of non-alcoholic fatty liver disease(NAFLD).[Methods]HepG2 cells were induced with oleic acid to establish a non-alcoholic fatty liver cell model.After intervention with 0.25 and 0.5 mg/mL of D.chinensis(L.)Nees.polysaccharides,the ALT and AST activity and TG and TC contents were detected with kits,and the changes in the expression of CDK5,TLR4,p-NF-κB and NF-κB were analyzed using Western-blotting.[Results]In the HepG2 cells induced with oleic acid,the ALT and AST activity increased significantly,the TG and TC contents increased significantly,and the expression levels of CDK5,TLR4 and p-NF-κB proteins up-regulated significantly.In the HepG2 cells intervened with D.chinensis(L.)Nees.polysaccharides,the activity of ALT and AST,the contents of TG and TC,and the expression levels of CDK5,TLR4 and p-NF-κB proteins all reduced significantly.[Conclusions]Polysaccharides from D.chinensis(L.)Nees.may interfere with NAFLD by inhibiting the TLR4/NF-κB pathway.展开更多
In experimental dogs,the effect of APN in alleviating the ischemiareperfusion injury was prominent.Compared with the sustained isthmian group,superoxide dismutast(SOD)in the ischemic region of myocardial tissue in the...In experimental dogs,the effect of APN in alleviating the ischemiareperfusion injury was prominent.Compared with the sustained isthmian group,superoxide dismutast(SOD)in the ischemic region of myocardial tissue in the ischemiareperfusion group was significantly decreased and malondialdehyde(MDA)marktdly increased Ca2+in myocardial cells was increased. and ultrastructural changes of myocardial tisstes were severe in the APN-pretreated ischemia-reperfusion group ,on the contrary,all the above parameters showed reversely,i,e.,SOD increased ,MDA and intracellular Ca2+ decreased.the ultrastructure changes were less distorted.展开更多
A new isocoumarin,tenuissimasatin（1）,was isolated from metabolites of the endophytic fungus Alternaria tenuissima（Nees ＆ T.Nees：Fr.） Wiltshire,which was isolated from the bark of Erythrophleum fordii Oliver,toge...A new isocoumarin,tenuissimasatin（1）,was isolated from metabolites of the endophytic fungus Alternaria tenuissima（Nees ＆ T.Nees：Fr.） Wiltshire,which was isolated from the bark of Erythrophleum fordii Oliver,together with 11 known compounds（2- 12）.The structure and absolute configuration of 1 was determined by means of NMR and CD analysis.Compound 3 shows selective cytotoxic activity on human colon cancer cell HCT-8（IC_（50） = 1.78μmol/L） by MTT test in vitro.Other compounds show no such activities（IC_（50）〉10μmol/L）.展开更多
运用冷冻切片、显微观察及显微照相的方法,对阴香[Cinnamomum burmanni(Nees et T.Nees)Blume]的茎结构进行了解剖研究。结果表明,阴香茎由周皮、皮层、韧皮部、木质部以及髓部5部分组成。茎横切面生长轮不明显,韧皮纤维发达,散孔材,管...运用冷冻切片、显微观察及显微照相的方法,对阴香[Cinnamomum burmanni(Nees et T.Nees)Blume]的茎结构进行了解剖研究。结果表明,阴香茎由周皮、皮层、韧皮部、木质部以及髓部5部分组成。茎横切面生长轮不明显,韧皮纤维发达,散孔材,管孔排列方式多为径向复管孔或孔团,管孔壁薄,平均厚度为4.04μm±0.58μm,平均弦向直径为7.64μm±2.57μm,导管平均数为(432.30±134.21)个/mm2。角质层厚薄不均,平均厚度为21.91μm±20.36μm。木射线为异形单列和异形I型。展开更多
研究了不同浓度纳他霉素对冬枣浆胞病菌Alternaria Nees ex Wallr．的室内毒力以及对冬枣低温贮藏期间生理生化指标的影响。结果表明，纳他霉素对冬枣浆胞病菌的EC50和EC90值分别为39．56和208．48mg／L，与对照药剂咪鲜胺、异菌脲相比...研究了不同浓度纳他霉素对冬枣浆胞病菌Alternaria Nees ex Wallr．的室内毒力以及对冬枣低温贮藏期间生理生化指标的影响。结果表明，纳他霉素对冬枣浆胞病菌的EC50和EC90值分别为39．56和208．48mg／L，与对照药剂咪鲜胺、异菌脲相比，EC90值无显著差异；经500～1000mg／L的纳他霉素处理可显著抑制冬枣丙二醛（MDA）含量的增加，并使多聚半乳糖醛酸酶（PG）与苯丙氨酸解氨酶（PAL）的活性提高，对保持果实硬度有很好的效果，并且抑制了可溶性固形物、抗坏血酸（Vc）含量的下降。经1000mg／L纳他霉素处理的冬枣贮藏到60d其商品率迭87．3％，高于对照组的商品率（72．9％）；贮藏到100d其商品率仍可达到75．0％，显著高于对照组（50．2％）。展开更多
利用组织透明法和石蜡切片法比较研究了楠属（Phoebe Nees）5种植物竹叶楠[Phoebe faberi（Hemsl.）Chun]、浙江楠（P.chekiangensis C B Shang）、闽楠[P.bournei（Hemsl.）Yang]、楠木（P.zhennan S.Lee et F N Wei）和紫楠[P.shearer...利用组织透明法和石蜡切片法比较研究了楠属（Phoebe Nees）5种植物竹叶楠[Phoebe faberi（Hemsl.）Chun]、浙江楠（P.chekiangensis C B Shang）、闽楠[P.bournei（Hemsl.）Yang]、楠木（P.zhennan S.Lee et F N Wei）和紫楠[P.sheareri（Hemsl.）Gamble]叶片中油细胞与黏液细胞的形状、直径、分布密度以及在叶肉中的具体分布。结果表明,5种楠属植物叶片中油细胞均为圆形,直径20~35μm;黏液细胞多为椭圆形,长轴直径30~75μm。2种细胞的分布密度在某些种类之间差异显著。从叶片横切面观察,5种植物的油细胞和黏液细胞均分布于栅栏组织,且靠近上表皮。展开更多
基金supported by the Department of Edu-cation(JA05238)the Key Project on Ecology of Fujian Province,China(0608537)
文摘Protein extraction is a critical step for two-dimensional electrophoresis （2-DE）. Different plant samples require different and adaptive protein extraction protocols. The leaves of medicinal plant, Baphicacanthus cusia （Nees） Bremek are notoriously recalcitrant to common protein extraction methods due to high levels of interfering compounds （especially the secondary metabolites and pigments）. This study was aimed to establish a routine procedure for the proteomic analysis ofB. cusia leaves, and a new protocol for the protein extraction was developed by optimizing trichloroacetic acid （TCA）/ acetone extraction method. The efficiency of this protocol was demonstrated by comparison with 3 published protein extraction methods （chloroform/acetone, Mg/NP-40, Tris-base/acetone）. The results showed that the optimized TCA/ acetone precipitation extraction method gave a relatively high protein yield （9.263 mg g^-1 fresh weight）, high-resolution separation, clear protein profiles, the highest proteins spots （1 31 t protein spots）, and displayed less contamination in 2- DE gels. Therefore, the results suggested that the optimized TCA/acetone method was the most effective among the 4 methods for B. cusia leaves.
文摘The aim of this experiment is to study the mechanism of APN in alleviating the Ca2+-overloading in dog model during the process of ischemic reperfusion. In comparison with the sustained ischemic group, the parameters in the ischemic reperfusion group demonstrated: Ca2+ of ischemic region of myocardial cell increased (P<0.05) , Na+ increased remarkably(P<0. 01), the activity of Ca2+-ATPase dropped remarkably(P<0. 01),and MDA increased significantly(P<0. 01). Whereas in the group pretreated with APN, the Ca2+ in the relevant area reduced (P<0.05), Na+ decreased significantly(P<0. 01), the activity of Ca2+-ATPase and Na+-K+ ATPase increased remarkably(P<0.01) ,and MDA decreased significantly (P<0. 01). These findings indicate that APN may improve the activity of sarcolemma ATPase in alleviating the Ca2+ and Na+-overloading by decreasing the harmful effect of oxygen free radicals.
基金Forestry Science and Technology Innovation Project of Guangdong Province(14KJCX005-02).
文摘Machilus spp.are mostly valuable timber and economic tree species,widely used in many fields such as artificial afforestation,landscaping,furniture and building materials,chemicals and medicine.In this article,the distribution,uses and domestic resources of Machilus Nees are summarized.Focus is placed on the analysis of propagation methods,including seed propagation,cutting propagation and tissue culture propagation.The research status and existing problems are summarized,and the future research focus on the propagation technology of Machilus Nees is prospected,with a view to provide theoretical references for the efficient breeding of high-quality seedlings and comprehensive promotion and application of Machilus spp.and contribute to the conservation and sustainable use of wild resources.
基金National Natural Science Foundation of China(81960779,81760114,81660104,81860673)Natural Science Foundation of Guangxi Zhuang Autonomous Region(2017GXNSFAA198218,2017GXNSFAA198326,2018GXNSFAA281040)。
文摘[Objectives]To observe the effects of polysaccharides from Dicliptera chinensis(L.)Nees.on the expression of TLR/NF-κB pathway related proteins in HepG2 cells induced by oleic acid,and to explore the possible mechanism of polysaccharides from D.chinensis(L.)Nees.in the treatment of non-alcoholic fatty liver disease(NAFLD).[Methods]HepG2 cells were induced with oleic acid to establish a non-alcoholic fatty liver cell model.After intervention with 0.25 and 0.5 mg/mL of D.chinensis(L.)Nees.polysaccharides,the ALT and AST activity and TG and TC contents were detected with kits,and the changes in the expression of CDK5,TLR4,p-NF-κB and NF-κB were analyzed using Western-blotting.[Results]In the HepG2 cells induced with oleic acid,the ALT and AST activity increased significantly,the TG and TC contents increased significantly,and the expression levels of CDK5,TLR4 and p-NF-κB proteins up-regulated significantly.In the HepG2 cells intervened with D.chinensis(L.)Nees.polysaccharides,the activity of ALT and AST,the contents of TG and TC,and the expression levels of CDK5,TLR4 and p-NF-κB proteins all reduced significantly.[Conclusions]Polysaccharides from D.chinensis(L.)Nees.may interfere with NAFLD by inhibiting the TLR4/NF-κB pathway.
文摘In experimental dogs,the effect of APN in alleviating the ischemiareperfusion injury was prominent.Compared with the sustained isthmian group,superoxide dismutast(SOD)in the ischemic region of myocardial tissue in the ischemiareperfusion group was significantly decreased and malondialdehyde(MDA)marktdly increased Ca2+in myocardial cells was increased. and ultrastructural changes of myocardial tisstes were severe in the APN-pretreated ischemia-reperfusion group ,on the contrary,all the above parameters showed reversely,i,e.,SOD increased ,MDA and intracellular Ca2+ decreased.the ultrastructure changes were less distorted.
基金Financial support from the National Science and Technology Project of China(No2009ZX09311-004)the Natural Science Foundation of China(No201072234)
文摘A new isocoumarin,tenuissimasatin（1）,was isolated from metabolites of the endophytic fungus Alternaria tenuissima（Nees ＆ T.Nees：Fr.） Wiltshire,which was isolated from the bark of Erythrophleum fordii Oliver,together with 11 known compounds（2- 12）.The structure and absolute configuration of 1 was determined by means of NMR and CD analysis.Compound 3 shows selective cytotoxic activity on human colon cancer cell HCT-8（IC_（50） = 1.78μmol/L） by MTT test in vitro.Other compounds show no such activities（IC_（50）〉10μmol/L）.
文摘运用冷冻切片、显微观察及显微照相的方法,对阴香[Cinnamomum burmanni(Nees et T.Nees)Blume]的茎结构进行了解剖研究。结果表明,阴香茎由周皮、皮层、韧皮部、木质部以及髓部5部分组成。茎横切面生长轮不明显,韧皮纤维发达,散孔材,管孔排列方式多为径向复管孔或孔团,管孔壁薄,平均厚度为4.04μm±0.58μm,平均弦向直径为7.64μm±2.57μm,导管平均数为(432.30±134.21)个/mm2。角质层厚薄不均,平均厚度为21.91μm±20.36μm。木射线为异形单列和异形I型。
文摘研究了不同浓度纳他霉素对冬枣浆胞病菌Alternaria Nees ex Wallr．的室内毒力以及对冬枣低温贮藏期间生理生化指标的影响。结果表明，纳他霉素对冬枣浆胞病菌的EC50和EC90值分别为39．56和208．48mg／L，与对照药剂咪鲜胺、异菌脲相比，EC90值无显著差异；经500～1000mg／L的纳他霉素处理可显著抑制冬枣丙二醛（MDA）含量的增加，并使多聚半乳糖醛酸酶（PG）与苯丙氨酸解氨酶（PAL）的活性提高，对保持果实硬度有很好的效果，并且抑制了可溶性固形物、抗坏血酸（Vc）含量的下降。经1000mg／L纳他霉素处理的冬枣贮藏到60d其商品率迭87．3％，高于对照组的商品率（72．9％）；贮藏到100d其商品率仍可达到75．0％，显著高于对照组（50．2％）。
文摘利用组织透明法和石蜡切片法比较研究了楠属（Phoebe Nees）5种植物竹叶楠[Phoebe faberi（Hemsl.）Chun]、浙江楠（P.chekiangensis C B Shang）、闽楠[P.bournei（Hemsl.）Yang]、楠木（P.zhennan S.Lee et F N Wei）和紫楠[P.sheareri（Hemsl.）Gamble]叶片中油细胞与黏液细胞的形状、直径、分布密度以及在叶肉中的具体分布。结果表明,5种楠属植物叶片中油细胞均为圆形,直径20~35μm;黏液细胞多为椭圆形,长轴直径30~75μm。2种细胞的分布密度在某些种类之间差异显著。从叶片横切面观察,5种植物的油细胞和黏液细胞均分布于栅栏组织,且靠近上表皮。
