This study aimed to analyze the partial sequence of COI gene in Pengze crucian carp,gold crucian carp,Ukraine carp and common carp,with silver carp and tilapia fish as two outgroups.DNA fragments 814 bp in length were...This study aimed to analyze the partial sequence of COI gene in Pengze crucian carp,gold crucian carp,Ukraine carp and common carp,with silver carp and tilapia fish as two outgroups.DNA fragments 814 bp in length were amplified and analyzed.Statistical analysis of sequence compositions and analysis of intraspecific and interspecific genetic distances were performed by using Mega4.1 software.The phylogenetic tree of COI gene was constructed with neighbor-joining method.According to the results,15 fish individuals could be divided into five groups,which was consistent with the results of traditional taxonomy.Furthermore,the partial sequence of COI gene was a good DNA barcode to identify different species and exhibited certain applicability in the analysis of interspecific phylogenetic relationships of Cyprinidae species.展开更多
Evergreen azaleas are among the most important ornamental shrubs in China.Today,there are probably over 300 cultivars preserved in different nurseries,but with little information available on the cultivar itself or re...Evergreen azaleas are among the most important ornamental shrubs in China.Today,there are probably over 300 cultivars preserved in different nurseries,but with little information available on the cultivar itself or relationships between cultivars.Amplified fragment length polymorphism(AFLP) markers were employed to determine the genetic relationships between evergreen azalea cultivars in China.One hundred and thirty genotypes collected from gardens and nurseries,including cultivars classified in the groups East,West,Hairy,and Summer,unknown cultivars,and close species,were analyzed using three primer pairs.A total of 408 polymorphic fragments were generated by AFLP reactions with an average of 136 fragments per primer pair.The average values of expected heterozygosity and Shannon's information index were 0.3395 and 0.5153,respectively.Genetic similarities were generated based on Dice coefficients,used to construct a neighbor joining tree,and bootstrapped for 100 replicates in Treecon V1.3b.Principal coordinate analysis(PCO) was performed based on Dice distances using NTSYS-pc software.The AFLP technique was useful for analyzing genetic diversity in evergreen azaleas.Cluster analysis revealed that cultivars in the West and Summer groups were quite distinct from other groups in the four-group classification system and that the East and Hairy groups should be redefined.展开更多
基金Supported by Program of Applied Basic Research and Cutting-edge Technology of Tianjin Municipality(Natural Science Foundation of Tianjin Municipality,11JCYBJC08200)
文摘This study aimed to analyze the partial sequence of COI gene in Pengze crucian carp,gold crucian carp,Ukraine carp and common carp,with silver carp and tilapia fish as two outgroups.DNA fragments 814 bp in length were amplified and analyzed.Statistical analysis of sequence compositions and analysis of intraspecific and interspecific genetic distances were performed by using Mega4.1 software.The phylogenetic tree of COI gene was constructed with neighbor-joining method.According to the results,15 fish individuals could be divided into five groups,which was consistent with the results of traditional taxonomy.Furthermore,the partial sequence of COI gene was a good DNA barcode to identify different species and exhibited certain applicability in the analysis of interspecific phylogenetic relationships of Cyprinidae species.
基金Project(No.2012C12909-7) supported by the Science and Technology Major Project of Zhejiang Province,China
文摘Evergreen azaleas are among the most important ornamental shrubs in China.Today,there are probably over 300 cultivars preserved in different nurseries,but with little information available on the cultivar itself or relationships between cultivars.Amplified fragment length polymorphism(AFLP) markers were employed to determine the genetic relationships between evergreen azalea cultivars in China.One hundred and thirty genotypes collected from gardens and nurseries,including cultivars classified in the groups East,West,Hairy,and Summer,unknown cultivars,and close species,were analyzed using three primer pairs.A total of 408 polymorphic fragments were generated by AFLP reactions with an average of 136 fragments per primer pair.The average values of expected heterozygosity and Shannon's information index were 0.3395 and 0.5153,respectively.Genetic similarities were generated based on Dice coefficients,used to construct a neighbor joining tree,and bootstrapped for 100 replicates in Treecon V1.3b.Principal coordinate analysis(PCO) was performed based on Dice distances using NTSYS-pc software.The AFLP technique was useful for analyzing genetic diversity in evergreen azaleas.Cluster analysis revealed that cultivars in the West and Summer groups were quite distinct from other groups in the four-group classification system and that the East and Hairy groups should be redefined.
