目的:探讨彝药黑根治疗类风湿性关节炎潜在的作用机制以及主要活性成分。方法:运用UPLC-Q-Orbitrap HRMS及GC-MS技术分析黑根的化学成分,利用Swiss Target进行活性成分的靶点筛选和预测,同时在Genecard、OMIM、Dis Ge NET等数据库中筛...目的:探讨彝药黑根治疗类风湿性关节炎潜在的作用机制以及主要活性成分。方法:运用UPLC-Q-Orbitrap HRMS及GC-MS技术分析黑根的化学成分,利用Swiss Target进行活性成分的靶点筛选和预测,同时在Genecard、OMIM、Dis Ge NET等数据库中筛选类风湿性关节炎相关靶点,利用Cytoscape3.9.0软件构建“活性成分-靶点”网络模型,运用String数据分析平台进行蛋白互作网络分析,并且利用基因富集分析在线工具对核心靶点进行GO富集分析和KEGG通路分析,最后使用分子对接对网络药理学内容进行初步验证。结果:共鉴定出黑根化学成分78个,筛选后活性成分50个,237个潜在作用靶点与类风湿性关节炎相关,包括STAT3、AKT1、EGFR等关键靶点。通路富集分析PI3K-Akt通路、JAK-STAT等信号通路可能是黑根发挥抗类风湿性关节炎的主要途径。通过分子对接技术得出主要活性成分(异绿原酸B、异绿原酸C)与关键靶点有较好结合力。结论:研究初步表明黑根通过多成分、多靶点、多途径治疗类风湿性关节炎的作用,为后续黑根物质基础研究提供了理论依据。展开更多
The contents of soluble sugar and soluble protein as well as PPO,POD and CAT activities in above-ground part of Huperzia serrata(Thunb.ex Murray) Trev.under different illumination conditions were analyzed.The results ...The contents of soluble sugar and soluble protein as well as PPO,POD and CAT activities in above-ground part of Huperzia serrata(Thunb.ex Murray) Trev.under different illumination conditions were analyzed.The results show that with the treating time(18 d) prolonging,these indexes appear different changing trends under different illumination conditions,and most of them are higher than those before treating.Soluble sugar content,soluble protein content,PPO activity and POD activity of H.serrata in 2 400 and 3 600 lx groups are obviously higher than that in 1 200 lx group,but CAT activity among the three illumination groups has no significant difference.It is concluded that the relative high illumination has a certain influence on some physiological-biochemical indexes of H.serrata,low illumination(1 200 lx) is relatively suitable for H.serrata growth.展开更多
文摘目的:探讨彝药黑根治疗类风湿性关节炎潜在的作用机制以及主要活性成分。方法:运用UPLC-Q-Orbitrap HRMS及GC-MS技术分析黑根的化学成分,利用Swiss Target进行活性成分的靶点筛选和预测,同时在Genecard、OMIM、Dis Ge NET等数据库中筛选类风湿性关节炎相关靶点,利用Cytoscape3.9.0软件构建“活性成分-靶点”网络模型,运用String数据分析平台进行蛋白互作网络分析,并且利用基因富集分析在线工具对核心靶点进行GO富集分析和KEGG通路分析,最后使用分子对接对网络药理学内容进行初步验证。结果:共鉴定出黑根化学成分78个,筛选后活性成分50个,237个潜在作用靶点与类风湿性关节炎相关,包括STAT3、AKT1、EGFR等关键靶点。通路富集分析PI3K-Akt通路、JAK-STAT等信号通路可能是黑根发挥抗类风湿性关节炎的主要途径。通过分子对接技术得出主要活性成分(异绿原酸B、异绿原酸C)与关键靶点有较好结合力。结论:研究初步表明黑根通过多成分、多靶点、多途径治疗类风湿性关节炎的作用,为后续黑根物质基础研究提供了理论依据。
文摘The contents of soluble sugar and soluble protein as well as PPO,POD and CAT activities in above-ground part of Huperzia serrata(Thunb.ex Murray) Trev.under different illumination conditions were analyzed.The results show that with the treating time(18 d) prolonging,these indexes appear different changing trends under different illumination conditions,and most of them are higher than those before treating.Soluble sugar content,soluble protein content,PPO activity and POD activity of H.serrata in 2 400 and 3 600 lx groups are obviously higher than that in 1 200 lx group,but CAT activity among the three illumination groups has no significant difference.It is concluded that the relative high illumination has a certain influence on some physiological-biochemical indexes of H.serrata,low illumination(1 200 lx) is relatively suitable for H.serrata growth.
