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Nested multiplex PCR for identification and detection of human Plasmodium species including Plasmodium knowlesi 被引量:1
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作者 Maria Miguel-Oteo Adela I Jiram +3 位作者 Thuy H Ta-Tang Marta Lanza Shamilah Hisam José Miguel Rubio 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2017年第3期280-284,共5页
Objective:To develop a new technique for diagnosis of Plasmodium knowlesi and at the same time to be able to discriminate among the diverse species of Plasmodium causing human malaria.Methods:In this study the nested ... Objective:To develop a new technique for diagnosis of Plasmodium knowlesi and at the same time to be able to discriminate among the diverse species of Plasmodium causing human malaria.Methods:In this study the nested multiplex malaria PCR was redesigned,targeting the 18S rR NA gene,to identify the fifth human Plasmodium species,Plasmodium knowlesi,together with the other human Plasmodium(Plasmodium falciparum,Plasmodium vivax,Plasmodium ovale and Plasmodium malariae)by amplified fragment size using only two amplification processes and including an internal reaction control to avoid false negatives.Results:The technique was validated with 91 clinical samples obtained from patients with malaria compatible symptoms.The technique showed high sensitivity(100%)and specificity(96%)when it was compared to the reference method employed for malaria diagnosis in the Instituto de Salud Carlos栿and a published real-time PCR malaria assay.Conclusions:The technique designed is an economical,sensitive and specific alternative to current diagnosis methods.Furthermore,the method might be tested in knowlesi-malaria endemic areas with a higher number of samples to confirm the quality of the method. 展开更多
关键词 MALARIA Plasmodium knowlesi nested multiplex pcr Molecular diagnosis
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