目的探讨妊娠早期血清丝氨酸蛋白酶抑制剂B1(SerpinB1)、神经调节蛋白4(NRG4)水平对妊娠期糖尿病(GDM)的预测价值。方法选取2020年12月至2022年2月在本院产检并分娩的161例GDM产妇作为GDM组,选取同期产检并分娩的152例非GDM产妇作为对...目的探讨妊娠早期血清丝氨酸蛋白酶抑制剂B1(SerpinB1)、神经调节蛋白4(NRG4)水平对妊娠期糖尿病(GDM)的预测价值。方法选取2020年12月至2022年2月在本院产检并分娩的161例GDM产妇作为GDM组,选取同期产检并分娩的152例非GDM产妇作为对照组。比较两组妊娠早期的血清SerpinB1、NRG4水平,分析其与其他血清学指标的相关性及对GDM发生和不良妊娠结局的影响及预测价值。结果GDM组的0、60、120 min口服葡萄糖耐量试验(OGTT)血糖、糖化血红蛋白(HbA1c)、甘油三酯(TG)及SerpinB1水平高于对照组,NRG4水平低于对照组(P<0.05)。妊娠早期血清SerpinB1水平与0、60、120 min OGTT血糖、HbA1c、TG呈正相关,与高密度脂蛋白胆固醇(HDL-C)呈负相关(P<0.05);妊娠早期血清NRG4水平与0、60、120 min OGTT血糖、HbA1c、低密度脂蛋白胆固醇(LDL-C)呈负相关,与HDL-C呈正相关(P<0.05)。Logistic回归分析结果显示,妊娠早期SerpinB1水平升高是GDM发生的危险因素(OR>1,P<0.05),NRG4水平升高是GDM发生的保护因素(OR<1,P<0.05)。受试者工作特征(ROC)曲线显示,血清SerpinB1、NRG4对GDM具有一定的预测价值。不良妊娠结局组的SerpinB1水平高于妊娠结局正常组,NRG4水平低于妊娠结局正常组(P<0.05)。Logistic回归分析结果显示,妊娠早期血清SerpinB1水平升高是GDM不良妊娠结局的危险因素(OR>1,P<0.05),NRG4水平升高是GDM不良妊娠结局的保护因素(OR<1,P<0.05)。ROC曲线显示,血清SerpinB1、NRG4对GDM不良妊娠结局具有一定的预测价值。结论妊娠早期血清SerpinB1、NRG4水平与GDM的发生及不良妊娠结局相关,在GDM发生及不良妊娠结局的预测中均有一定的临床价值。展开更多
Studies on ischemia/reperfusion(I/R)injury suggest that exogenous neural stem cells(NSCs)are ideal candidates for stem cell therapy reperfusion injury.However,NSCs are difficult to obtain owing to ethical limitations....Studies on ischemia/reperfusion(I/R)injury suggest that exogenous neural stem cells(NSCs)are ideal candidates for stem cell therapy reperfusion injury.However,NSCs are difficult to obtain owing to ethical limitations.In addition,the survival,differentiation,and proliferation rates of transplanted exogenous NSCs are low,which limit their clinical application.Our previous study showed that neuregulin1β(NRG1β)alleviated cerebral I/R injury in rats.In this study,we aimed to induce human umbilical cord mesenchymal stem cells into NSCs and investigate the improvement effect and mechanism of NSCs pretreated with 10 nM NRG1βon PC12 cells injured by oxygen-glucose deprivation/reoxygenation(OGD/R).Our results found that 5 and 10 nM NRG1βpromoted the generation and proliferation of NSCs.Co-culture of NSCs and PC12 cells under condition of OGD/R showed that pretreatment of NSCs with NRG1βimproved the level of reactive oxygen species,malondialdehyde,glutathione,superoxide dismutase,nicotinamide adenine dinucleotide phosphate,and nuclear factor erythroid 2-related factor 2(Nrf2)and mitochondrial damage in injured PC12 cells;these indexes are related to ferroptosis.Research has reported that p53 and solute carrier family 7 member 11(SLC7A11)play vital roles in ferroptosis caused by cerebral I/R injury.Our data show that the expression of p53 was increased and the level of glutathione peroxidase 4(GPX4)was decreased after RNA interference-mediated knockdown of SLC7A11 in PC12 cells,but this change was alleviated after co-culturing NSCs with damaged PC12 cells.These findings suggest that NSCs pretreated with NRG1βexhibited neuroprotective effects on PC12 cells subjected to OGD/R through influencing the level of ferroptosis regulated by p53/SLC7A11/GPX4 pathway.展开更多
文摘目的探讨妊娠早期血清丝氨酸蛋白酶抑制剂B1(SerpinB1)、神经调节蛋白4(NRG4)水平对妊娠期糖尿病(GDM)的预测价值。方法选取2020年12月至2022年2月在本院产检并分娩的161例GDM产妇作为GDM组,选取同期产检并分娩的152例非GDM产妇作为对照组。比较两组妊娠早期的血清SerpinB1、NRG4水平,分析其与其他血清学指标的相关性及对GDM发生和不良妊娠结局的影响及预测价值。结果GDM组的0、60、120 min口服葡萄糖耐量试验(OGTT)血糖、糖化血红蛋白(HbA1c)、甘油三酯(TG)及SerpinB1水平高于对照组,NRG4水平低于对照组(P<0.05)。妊娠早期血清SerpinB1水平与0、60、120 min OGTT血糖、HbA1c、TG呈正相关,与高密度脂蛋白胆固醇(HDL-C)呈负相关(P<0.05);妊娠早期血清NRG4水平与0、60、120 min OGTT血糖、HbA1c、低密度脂蛋白胆固醇(LDL-C)呈负相关,与HDL-C呈正相关(P<0.05)。Logistic回归分析结果显示,妊娠早期SerpinB1水平升高是GDM发生的危险因素(OR>1,P<0.05),NRG4水平升高是GDM发生的保护因素(OR<1,P<0.05)。受试者工作特征(ROC)曲线显示,血清SerpinB1、NRG4对GDM具有一定的预测价值。不良妊娠结局组的SerpinB1水平高于妊娠结局正常组,NRG4水平低于妊娠结局正常组(P<0.05)。Logistic回归分析结果显示,妊娠早期血清SerpinB1水平升高是GDM不良妊娠结局的危险因素(OR>1,P<0.05),NRG4水平升高是GDM不良妊娠结局的保护因素(OR<1,P<0.05)。ROC曲线显示,血清SerpinB1、NRG4对GDM不良妊娠结局具有一定的预测价值。结论妊娠早期血清SerpinB1、NRG4水平与GDM的发生及不良妊娠结局相关,在GDM发生及不良妊娠结局的预测中均有一定的临床价值。
基金supported by the National Natural Science Foundation of China,No.81973501the Natural Science Foundation of Shandong Province,No.ZR2019MH009(both to YLG).
文摘Studies on ischemia/reperfusion(I/R)injury suggest that exogenous neural stem cells(NSCs)are ideal candidates for stem cell therapy reperfusion injury.However,NSCs are difficult to obtain owing to ethical limitations.In addition,the survival,differentiation,and proliferation rates of transplanted exogenous NSCs are low,which limit their clinical application.Our previous study showed that neuregulin1β(NRG1β)alleviated cerebral I/R injury in rats.In this study,we aimed to induce human umbilical cord mesenchymal stem cells into NSCs and investigate the improvement effect and mechanism of NSCs pretreated with 10 nM NRG1βon PC12 cells injured by oxygen-glucose deprivation/reoxygenation(OGD/R).Our results found that 5 and 10 nM NRG1βpromoted the generation and proliferation of NSCs.Co-culture of NSCs and PC12 cells under condition of OGD/R showed that pretreatment of NSCs with NRG1βimproved the level of reactive oxygen species,malondialdehyde,glutathione,superoxide dismutase,nicotinamide adenine dinucleotide phosphate,and nuclear factor erythroid 2-related factor 2(Nrf2)and mitochondrial damage in injured PC12 cells;these indexes are related to ferroptosis.Research has reported that p53 and solute carrier family 7 member 11(SLC7A11)play vital roles in ferroptosis caused by cerebral I/R injury.Our data show that the expression of p53 was increased and the level of glutathione peroxidase 4(GPX4)was decreased after RNA interference-mediated knockdown of SLC7A11 in PC12 cells,but this change was alleviated after co-culturing NSCs with damaged PC12 cells.These findings suggest that NSCs pretreated with NRG1βexhibited neuroprotective effects on PC12 cells subjected to OGD/R through influencing the level of ferroptosis regulated by p53/SLC7A11/GPX4 pathway.
