Objective The aim of this study was to determine Neuropilin 1(NRP1)contribution to transforming growth factorβ1(TGF-β1)-induced epithelial mesenchymal transition(EMT)of HGC-27 gastric cancer cells and study its mech...Objective The aim of this study was to determine Neuropilin 1(NRP1)contribution to transforming growth factorβ1(TGF-β1)-induced epithelial mesenchymal transition(EMT)of HGC-27 gastric cancer cells and study its mechanism.Methods In this study,TGF-β1 was used to induce EMT in HGC-27 cells.Further,these cells were stably transfected with siRNA targeting NRP1.Wound healing and transwell assays were used to measure cell migration and invasion,respectively.NRP1 and EMT markers were measured using quantitative real time reverse transcription polymerase chain reaction and western blotting.Results Exposure of TGF-β1 conferred a fibroblastic-like shape to cancer cells and significantly increased the expression of NRP1 in HGC-27 cells.TGF-β1 subsequently promoted migration and invasion of HGC-27 cells.Furthermore,silencing NRP1 inhibited the invasion and migration of TGF-β1-induced cells undergoing EMT.Conclusion Silencing NRP1 can inhibit cell migration,invasion,and metastasis and reverse the TGF-β1-induced EMT process of gastric cancer.展开更多
目的运用CiteSpace分析神经纤毛蛋白-1(NRP1)的研究现状、热点和趋势。方法检索中国知网、万方数据知识服务平台、维普网及Web of Science核心合集数据库从建库至2022年9月NRP1的相关文献,利用CiteSpace对年份、作者、机构及关键词进行...目的运用CiteSpace分析神经纤毛蛋白-1(NRP1)的研究现状、热点和趋势。方法检索中国知网、万方数据知识服务平台、维普网及Web of Science核心合集数据库从建库至2022年9月NRP1的相关文献,利用CiteSpace对年份、作者、机构及关键词进行可视化分析。结果共纳入中文文献536篇和英文文献2444篇,年发文量呈现波动上升趋势。中文文献发文量最多的作者是颜江华和韩正祥,英文作者是Ruhrberg,Christiana。厦门大学和哈佛大学分别是中英文文献发文量最多的机构。胃癌、血管生成、乳腺癌是中文研究热点,表达、受体、内皮生长因子是英文研究热点。结论NRP1的研究仍处于发展阶段,研究热点和趋势是机制和临床研究。团队间应加强合作,使研究更为系统。展开更多
基金Supported by grants from Returning Overseas Students(No.CY201721).
文摘Objective The aim of this study was to determine Neuropilin 1(NRP1)contribution to transforming growth factorβ1(TGF-β1)-induced epithelial mesenchymal transition(EMT)of HGC-27 gastric cancer cells and study its mechanism.Methods In this study,TGF-β1 was used to induce EMT in HGC-27 cells.Further,these cells were stably transfected with siRNA targeting NRP1.Wound healing and transwell assays were used to measure cell migration and invasion,respectively.NRP1 and EMT markers were measured using quantitative real time reverse transcription polymerase chain reaction and western blotting.Results Exposure of TGF-β1 conferred a fibroblastic-like shape to cancer cells and significantly increased the expression of NRP1 in HGC-27 cells.TGF-β1 subsequently promoted migration and invasion of HGC-27 cells.Furthermore,silencing NRP1 inhibited the invasion and migration of TGF-β1-induced cells undergoing EMT.Conclusion Silencing NRP1 can inhibit cell migration,invasion,and metastasis and reverse the TGF-β1-induced EMT process of gastric cancer.
文摘目的运用CiteSpace分析神经纤毛蛋白-1(NRP1)的研究现状、热点和趋势。方法检索中国知网、万方数据知识服务平台、维普网及Web of Science核心合集数据库从建库至2022年9月NRP1的相关文献,利用CiteSpace对年份、作者、机构及关键词进行可视化分析。结果共纳入中文文献536篇和英文文献2444篇,年发文量呈现波动上升趋势。中文文献发文量最多的作者是颜江华和韩正祥,英文作者是Ruhrberg,Christiana。厦门大学和哈佛大学分别是中英文文献发文量最多的机构。胃癌、血管生成、乳腺癌是中文研究热点,表达、受体、内皮生长因子是英文研究热点。结论NRP1的研究仍处于发展阶段,研究热点和趋势是机制和临床研究。团队间应加强合作,使研究更为系统。