AIM:To investigate whether cocktail theraphy combined with of neuroprotectants may have more advantages over single agents in treating focal cerebral ischemic cascade.METHODS:With the use of suture occlusin techique,t...AIM:To investigate whether cocktail theraphy combined with of neuroprotectants may have more advantages over single agents in treating focal cerebral ischemic cascade.METHODS:With the use of suture occlusin techique,the right middle cerebral artery in rats was occluded.Tirty minutes later,Frutose 1,6 diphosphate(FDP) (50 mg/kg,n=20), MK 801(1 mg/kg,n=20) and N acetylcystein (NAC)(150 mg/kg,n=20) were singly or combinantly infused intraperitoneally.At the same time the cocktail treated group(n=20)were infused the above agents combinationly and the control group(n=20)were infused normal saline intraperitoneally.6 hours and 24 hours after focal cerebral ischemia the animals were weighted and neurologically assessed on 5 point scale.The animals were killed,brains were stained 2,3,5 triphenyltetrazolium chloride for assessment of the infarct volume and then embedden onto slides with paraffin for morphological assessment and terminal transferase dUTP nick ending labeling(TUNEL )were carried out for apoptosis and immunohistochemistry were carried out to investigate the changes in bcl 2.RESULTS:All Neuroprotectants decreased volume of infarction (P< 0.05,F test).While cocktail treated group showed more distinct decrease than other groups(P< 0.05,F test).FDP treated group did not decrease the apoptosis of the neurons and did not increase the bcl 2 expression as well.MK 801 treated group,NAC treated group and cocktail treated group significantly decreased the apoptosis of the neurons and increased the bcl 2 expression (P< 0.05,F test).With cocktail treated group showing more distinct effect (P< 0.05,F test).CONCLUSION:Cocktail may be more effective than single neuroprotectant in this modle.展开更多
Background:Licochalcone A(LCA)is a flavonoid derived from the roots of Glycyrrhiza uralensis Fisch.ex DC and other related plants.Its natural source has been extensively used in complementary medicine,especially tradi...Background:Licochalcone A(LCA)is a flavonoid derived from the roots of Glycyrrhiza uralensis Fisch.ex DC and other related plants.Its natural source has been extensively used in complementary medicine,especially traditional Chinese medicine,to treat various ailments.Identified as a phenolic chalcone compound,LCA has gained significant attention in recent years due to its various pharmacological properties.Objective:The objective of this review article is to assess the anti-aging ralated pharmacological properties of LCA.Method:An exhaustive search of several scientific databases was conducted using various relevant keywords to write this review article on LCA,focusing on its therapeutic applications and anti-aging-related pharmacological activities.Some inclusion and exclusion criteria were strictly followed to retrieve updated relevant articles.Results:A collection of 292 papers were screened,and 131 were included in this review for an in-depth analysis of Licochalcone A,focusing on its anti-aging-related pharmacological activities and relevant mechanisms,and its toxicity and side effects.Moreover,the potential of LCA as a pharmacological product is discussed,emphasizing its health benefits and potential as a pharmaceutical product.Conclusion:These findings indicate that Licochalcone A is a promising natural therapeutic agent for anti-aging therapy and other ailments.展开更多
文摘AIM:To investigate whether cocktail theraphy combined with of neuroprotectants may have more advantages over single agents in treating focal cerebral ischemic cascade.METHODS:With the use of suture occlusin techique,the right middle cerebral artery in rats was occluded.Tirty minutes later,Frutose 1,6 diphosphate(FDP) (50 mg/kg,n=20), MK 801(1 mg/kg,n=20) and N acetylcystein (NAC)(150 mg/kg,n=20) were singly or combinantly infused intraperitoneally.At the same time the cocktail treated group(n=20)were infused the above agents combinationly and the control group(n=20)were infused normal saline intraperitoneally.6 hours and 24 hours after focal cerebral ischemia the animals were weighted and neurologically assessed on 5 point scale.The animals were killed,brains were stained 2,3,5 triphenyltetrazolium chloride for assessment of the infarct volume and then embedden onto slides with paraffin for morphological assessment and terminal transferase dUTP nick ending labeling(TUNEL )were carried out for apoptosis and immunohistochemistry were carried out to investigate the changes in bcl 2.RESULTS:All Neuroprotectants decreased volume of infarction (P< 0.05,F test).While cocktail treated group showed more distinct decrease than other groups(P< 0.05,F test).FDP treated group did not decrease the apoptosis of the neurons and did not increase the bcl 2 expression as well.MK 801 treated group,NAC treated group and cocktail treated group significantly decreased the apoptosis of the neurons and increased the bcl 2 expression (P< 0.05,F test).With cocktail treated group showing more distinct effect (P< 0.05,F test).CONCLUSION:Cocktail may be more effective than single neuroprotectant in this modle.
文摘Background:Licochalcone A(LCA)is a flavonoid derived from the roots of Glycyrrhiza uralensis Fisch.ex DC and other related plants.Its natural source has been extensively used in complementary medicine,especially traditional Chinese medicine,to treat various ailments.Identified as a phenolic chalcone compound,LCA has gained significant attention in recent years due to its various pharmacological properties.Objective:The objective of this review article is to assess the anti-aging ralated pharmacological properties of LCA.Method:An exhaustive search of several scientific databases was conducted using various relevant keywords to write this review article on LCA,focusing on its therapeutic applications and anti-aging-related pharmacological activities.Some inclusion and exclusion criteria were strictly followed to retrieve updated relevant articles.Results:A collection of 292 papers were screened,and 131 were included in this review for an in-depth analysis of Licochalcone A,focusing on its anti-aging-related pharmacological activities and relevant mechanisms,and its toxicity and side effects.Moreover,the potential of LCA as a pharmacological product is discussed,emphasizing its health benefits and potential as a pharmaceutical product.Conclusion:These findings indicate that Licochalcone A is a promising natural therapeutic agent for anti-aging therapy and other ailments.
