A 3-year-old boy presented with bluish patch and scattered blue spots on the left side of his face.After several sessions of laser treatment,the azury patch in the periorbital area became even darker.Histopathology sh...A 3-year-old boy presented with bluish patch and scattered blue spots on the left side of his face.After several sessions of laser treatment,the azury patch in the periorbital area became even darker.Histopathology showed many bipolar,pigment-laden dendritic cells scattered in the papillary and upper reticular dermis.Immunohistochemically,these cells were positive for S100,SOX-10,melan-A,P16,and HMB-45.The positive rate of Ki-67 was less than 5%.Finally,the lesion was diagnosed with nevus of Ota concurrent with common blue nevus.Therefore,for cases of the nevus of Ota with poor response to laser treatment,the possible coexisting diseases should be suspected.展开更多
To evaluate the clinical response of Nevus of Ota to Q switched Alexandrite las er, and analyze factors that influence the treatment outcome Methods A total of 522 patients treated with Q switched Alexandrite lase...To evaluate the clinical response of Nevus of Ota to Q switched Alexandrite las er, and analyze factors that influence the treatment outcome Methods A total of 522 patients treated with Q switched Alexandrite laser were included in the study Single and multiple variate analyses of various factors were per formed Results Satisfactory result was observed in all patients, none of whom developed scarrin g Clinical response was improved with additional treatment sessions The clin ical response of the 20-27 week treatment interval group was significantly better t han that of the 12-19 week interval group, but showed no significant difference as compared with both the 28-35 and ≥36 week interval group Zygomatic, bucc al and frontal areas showed better response than ocular and temporal areas Tre atment session, interval, and fluence were significant factors identified by mul tivariate analysis Conclusions Q switched Alexandrite laser is an ideal method for treating Nevus of Ota witho ut injury The number of treatment sessions is more important than interval or fluence展开更多
To investigate injury to epidermal melanocyte by Q-switched Alexandrite laser Methods Multiple biopsies were performed on 5 patients with nevus of Ota from before irradiation to 1 year after irradiation Fourteen s...To investigate injury to epidermal melanocyte by Q-switched Alexandrite laser Methods Multiple biopsies were performed on 5 patients with nevus of Ota from before irradiation to 1 year after irradiation Fourteen specimens were obtained for light microscopy, and 17 for transmission electron microscopy Results Melanosomes in epidermal melanocytes were both smaller in size and fewer in number than those in dermal melanocytes Immediately after irradiation, focal extracellular vacuoles of the basal layer could be observed under light microscopy Most epidermal melanocytes underwent mild or moderate injury in the form of vacuolated melanosomes, swollen mitochondria, dilation of endoplasmic reticulum, and expansion of extracellular space, retaining intact cell membranes Normal structures were restored 5 months to 1 year after irradiation, with no depigmentation or hyperpigmentation as seen by light microscopy Conclusion Injury of melanosomes in epidermal melanocytes is reversible展开更多
Background The nevus of Ota, is a common benign pigmentary dermatosis, mainly involve innervation area of first and second branch of trigeminal nerve. The classification of nevus of Ota was proposed by Tanino, based o...Background The nevus of Ota, is a common benign pigmentary dermatosis, mainly involve innervation area of first and second branch of trigeminal nerve. The classification of nevus of Ota was proposed by Tanino, based on 26 cases of nevus of Ota from 1937 to 1940. Studies about its classification are rarely seen in last 70 years, while it is still practical today.展开更多
In order to determine the appropriate treatment interval, 267 patients who underwent 3 sessions of treatment with Q-switched alexandrite laser were divided into 4 groups according to treatment interval, and their clin...In order to determine the appropriate treatment interval, 267 patients who underwent 3 sessions of treatment with Q-switched alexandrite laser were divided into 4 groups according to treatment interval, and their clinical responses were compared. Among them, 187 were asked about the process of pigment fading. Moreover, light and transmission electron microscopy were performed. It was noted that the clinical response of the 5 - 6 month interval group was significantly better than that of the 3 - 4 month group, but showed no significant difference from that of the 7 - 8 or ≥ 9 month group. 80.21% of investigated patients stated that marked pigment fading could no longer be observed 7 months after irradiation. 4 months after irradiation, the degenerated melanosomes and cell debris were still scattered among collagen fibers, scavenged gradually by macrophage. In conclusion, an appropriate treatment interval is 5 - 6 months.展开更多
OBJECTIVE: To investigate the effect of Q-switched alexandrite laser irradiation on dermal melanocytes of nevus of Ota. METHODS: Multiple biopsies were carried out on 4 patients with nevus of Ota before and after lase...OBJECTIVE: To investigate the effect of Q-switched alexandrite laser irradiation on dermal melanocytes of nevus of Ota. METHODS: Multiple biopsies were carried out on 4 patients with nevus of Ota before and after laser irradiation. Altogether 11 samples were examined under light microscope and 14 under transmission electron microscope. RESULTS: Immediately after laser irradiation, the dermal melanocytes were destroyed, the melanosomes were degenerated with central vesicle formation within most of them, and intradermal round vacuoles appeared. The epidermis remained intact. Three months to 1 year after irradiation, the degenerated melanosomes and cell debris were scavenged mainly by macrophages. Dermal melanocytes gradually decreased. No fibrosis was found. CONCLUSION: Q-switched alexandrite laser can selectively destroy dermal melanocytes of nevus of Ota and treat the disease safely.展开更多
基金This study was funded by the CAMS Innovation Fund for Medical Sciences(CIFMS-2021-I2M-1-001)National Natural Science Foundation of China(82103705).
文摘A 3-year-old boy presented with bluish patch and scattered blue spots on the left side of his face.After several sessions of laser treatment,the azury patch in the periorbital area became even darker.Histopathology showed many bipolar,pigment-laden dendritic cells scattered in the papillary and upper reticular dermis.Immunohistochemically,these cells were positive for S100,SOX-10,melan-A,P16,and HMB-45.The positive rate of Ki-67 was less than 5%.Finally,the lesion was diagnosed with nevus of Ota concurrent with common blue nevus.Therefore,for cases of the nevus of Ota with poor response to laser treatment,the possible coexisting diseases should be suspected.
文摘To evaluate the clinical response of Nevus of Ota to Q switched Alexandrite las er, and analyze factors that influence the treatment outcome Methods A total of 522 patients treated with Q switched Alexandrite laser were included in the study Single and multiple variate analyses of various factors were per formed Results Satisfactory result was observed in all patients, none of whom developed scarrin g Clinical response was improved with additional treatment sessions The clin ical response of the 20-27 week treatment interval group was significantly better t han that of the 12-19 week interval group, but showed no significant difference as compared with both the 28-35 and ≥36 week interval group Zygomatic, bucc al and frontal areas showed better response than ocular and temporal areas Tre atment session, interval, and fluence were significant factors identified by mul tivariate analysis Conclusions Q switched Alexandrite laser is an ideal method for treating Nevus of Ota witho ut injury The number of treatment sessions is more important than interval or fluence
文摘To investigate injury to epidermal melanocyte by Q-switched Alexandrite laser Methods Multiple biopsies were performed on 5 patients with nevus of Ota from before irradiation to 1 year after irradiation Fourteen specimens were obtained for light microscopy, and 17 for transmission electron microscopy Results Melanosomes in epidermal melanocytes were both smaller in size and fewer in number than those in dermal melanocytes Immediately after irradiation, focal extracellular vacuoles of the basal layer could be observed under light microscopy Most epidermal melanocytes underwent mild or moderate injury in the form of vacuolated melanosomes, swollen mitochondria, dilation of endoplasmic reticulum, and expansion of extracellular space, retaining intact cell membranes Normal structures were restored 5 months to 1 year after irradiation, with no depigmentation or hyperpigmentation as seen by light microscopy Conclusion Injury of melanosomes in epidermal melanocytes is reversible
文摘Background The nevus of Ota, is a common benign pigmentary dermatosis, mainly involve innervation area of first and second branch of trigeminal nerve. The classification of nevus of Ota was proposed by Tanino, based on 26 cases of nevus of Ota from 1937 to 1940. Studies about its classification are rarely seen in last 70 years, while it is still practical today.
基金This work was supported by the Shanghai Municipal Natural Science under the Grant (97-181).
文摘In order to determine the appropriate treatment interval, 267 patients who underwent 3 sessions of treatment with Q-switched alexandrite laser were divided into 4 groups according to treatment interval, and their clinical responses were compared. Among them, 187 were asked about the process of pigment fading. Moreover, light and transmission electron microscopy were performed. It was noted that the clinical response of the 5 - 6 month interval group was significantly better than that of the 3 - 4 month group, but showed no significant difference from that of the 7 - 8 or ≥ 9 month group. 80.21% of investigated patients stated that marked pigment fading could no longer be observed 7 months after irradiation. 4 months after irradiation, the degenerated melanosomes and cell debris were still scattered among collagen fibers, scavenged gradually by macrophage. In conclusion, an appropriate treatment interval is 5 - 6 months.
文摘OBJECTIVE: To investigate the effect of Q-switched alexandrite laser irradiation on dermal melanocytes of nevus of Ota. METHODS: Multiple biopsies were carried out on 4 patients with nevus of Ota before and after laser irradiation. Altogether 11 samples were examined under light microscope and 14 under transmission electron microscope. RESULTS: Immediately after laser irradiation, the dermal melanocytes were destroyed, the melanosomes were degenerated with central vesicle formation within most of them, and intradermal round vacuoles appeared. The epidermis remained intact. Three months to 1 year after irradiation, the degenerated melanosomes and cell debris were scavenged mainly by macrophages. Dermal melanocytes gradually decreased. No fibrosis was found. CONCLUSION: Q-switched alexandrite laser can selectively destroy dermal melanocytes of nevus of Ota and treat the disease safely.
