The Congolese population is organised into households, which are thus headed by a chief who ensures the social well-being, development and integration into working life of the individuals in his charge. This study exa...The Congolese population is organised into households, which are thus headed by a chief who ensures the social well-being, development and integration into working life of the individuals in his charge. This study examines the functional principles of new housing design as an instrument for transforming the (current) failing economy into a strong and resilient one. Accordingly, a literature review of the practice of designing and building housing in human settlements in the Congo revealed the state of the art on this subject. An analysis of the existing housing stock from a demographic, social and economic point of view made it possible to identify the most common household sizes and numbers, as well as the lifestyle processes that determine the need for developed space. To this end, the experimental method was used to propose configuration plans for various new types of dwelling. To this end, the study highlighted the link between people’s standard of living and the effectiveness of their involvement in the local economy. To achieve the aims of the National Development Plan (NDP), particular attention must be paid to solving the housing problem. The existing housing stock actively contributes to the problems associated with unemployment and insecurity. Functional principles for the design of new types of housing have been developed. Four model types are proposed in line with the demographic structure of the population, their socio-economic characteristics and their lifestyle.展开更多
AIM: To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells. METHODS: The apoptosis of duck embryo fibroblasts (DEF) i...AIM: To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells. METHODS: The apoptosis of duck embryo fibroblasts (DEF) induced by NGVEV was investigated by fluorescence-activated cell sorter (FACS) and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide (PI). RESULTS: By staining cells with a combination of fluorescein annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells (Annexin V-FITC negative/PI negative), early apoptotic cells (Annexin V-FITC positive/PI negative), late apoptotic/necrotic cells (Annexin V-FITC positive/ PI positive) and dead cells (Annexin V-FITC negative/PI positive) through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non- apoptotic cells decreased.展开更多
Anatase and its allomorphic mineral rutile have the most prominent economic significance among titanium mineral resources and constitute one of the badly needed mineral resources currently in China. The Yantizishan-Mo...Anatase and its allomorphic mineral rutile have the most prominent economic significance among titanium mineral resources and constitute one of the badly needed mineral resources currently in China. The Yantizishan-Moshishan anatase deposit was formerly referred to as an iron deposit. Based on recent investigation and exploration the authors believe that it is actually a large metamorphosed sedimentary anatase-dominated deposit belonging to a new genetic type. Ore bodies occur in stratoid and lenticular forms in Mesoproterozoic (1751 Ma) schist, metasandstone (metasiltstone), and amphibolite. Rich ores have perthitic structure comprising chiefly interbedded quartz perthite (with disseminated anatase and rutile) and anatase perthite. Ore minerals are mainly anatase and subordinately rutile and ilmenite (±hematite), while nonmetallic minerals are chiefly quartz with a certain amount of anthophyllite and biotite (±garnet). The grain sizes of anatase, ruffle and ilmenite are 0.01-0.1 mm. Rich ores contain 3.14% to 15.46% TiO2. averaging 6.91%, while the low-grade ores have TiO2 content about 1.2%to 2.97%, averaging 1.76%. The ores have relatively high TFe and V contents. Trace elements in anatase and rutile such as Nb and Cr were analyzed by the electron microprobe. According to their relatively low Nb and Cr contents, source anatase and rutile must have come from meta-mafic rocks. Trace elements of the associated ilmenite show relatively high MnO and low MgO contents, just in contrast to those of ilmenite in V-Ti-magnetite ores of magmatic origin. The protoliths of amphibolite wall rocks should be basalt and picrite-basalt. Pertochemical data suggest that the tectonic setting of these rocks belongs to an island arc or a transitional belt between the island arc and oceanic ridge. Silicon isotope study shows that δ30Si values of different anatase ores, quartzite, and schist in this deposit are 0.1‰ to -0.9‰, similar to those of marine hydrothermal exhalative sedimentary deposits. All of these geological and geochemical characteristics of the ore deposit suggest that the anatase ores and amphibolite are products of submarine basic volcanism. The ores had chemical precipitation features, but were later subjected to regional intermediate (or somewhat lower) grade metamorphism (1158 Ma). Rutile was formed mainly in the process of this metamorphism. The ore belt locally underwent hydrothermal modification during the emplacement of Late Yanshanian granite (118 Ma).展开更多
Both new plant type (NPT) and intersubspecific hybrid rice (IHR) had large sink size (total number of spikelets per m2), however, poor grain filling limited their potential in the grain yield. Compared to the three-li...Both new plant type (NPT) and intersubspecific hybrid rice (IHR) had large sink size (total number of spikelets per m2), however, poor grain filling limited their potential in the grain yield. Compared to the three-line indica hybrid of Shanyou 63 (CK), NPT and IHR showed higher photosynthetic potential, higher dry matter accumulation and higher ratio of dry weight to spikelets (total dry wt./total number of spikelets) from heading to harvest. But both exhibited a low export percentage and transfer ratio of assimilates, low partitioning of 14C to grains from labeled flag leaves, low harvest indices and low physiological activities (IAA content and activities of ATPase and starch synthase) of grains at early grain-filling stage. The physiological activities of grains at early filling stage were significantly correlated with the export percentage and transfer ratio of assimilates, ripened-grain percentage and grain plumpness (r = 0.85 - 0.95). The source-sink ratio (dry matter wt./spikelet and nonstructural carbohydrate/spikelet) at heading was positively correlated with physiological activities of grains (r = 0. 84 - 0. 97 ). It is suggested that low physiological activities of grains at early filling stage is attributed to low source-sink ratio at heading, and the low sink activity weakens the ability to remobilize assimilates into grains, and leads to poor grain filling in NPT and IHR.展开更多
In this paper, a photoelectric signal obtained scheme via equator triangle pattern engraved on rotor is discussed and the mathematic model is deduced in the case which is deflexion between rotor axis and the coordinat...In this paper, a photoelectric signal obtained scheme via equator triangle pattern engraved on rotor is discussed and the mathematic model is deduced in the case which is deflexion between rotor axis and the coordinate frame of case. The deflexion error and coupling error under the situation are analyzed. Finally, three methods of engraving based on the spherical triangle pattern are presented. The error models of various methods are built up and the simulation curves are provided respectively. We have done the primary experiments on the surface of rotor using this method. It can be seen from the enlarged figures that the rim of the pattern is smooth and the demand of sensor resolution is satisfied by and large. The results of study supply reference for signal obtaining.展开更多
Numerical simulation was used to estimate the temperature field within a poured concrete,mono-layer freezing-shaft lining.The affects from various factors were investigated.The maximum temperature within the lining in...Numerical simulation was used to estimate the temperature field within a poured concrete,mono-layer freezing-shaft lining.The affects from various factors were investigated.The maximum temperature within the lining increases as the lining thickness increases,decreases as the soil-side wall temperature decreases,decreases as the air temperature inside the shaft decreases and decreases as the air velocity inside the shaft increases.The compression speed of an insulating foam layer affects the maximum temperature difference between the interior and the sidewalls.The maximum temperature difference between the interior and the sidewalls approaches or exceeds the maximum allowable for the curing of poured concrete structures.Attention should be paid to the question of the lining cracking during the curing period.The temperature gradient in the vertical direction may be minimized by preventing air contact against the steel connection board supporting the base of the freshly poured section.展开更多
The South China Sea (SCS), in particular the northern SCS, is one of ocean areas where energetic internal solitary waves (ISWs)occur most frequently (Cai et al., 2012; Zheng, 2017). Based on the re-appearance pe...The South China Sea (SCS), in particular the northern SCS, is one of ocean areas where energetic internal solitary waves (ISWs)occur most frequently (Cai et al., 2012; Zheng, 2017). Based on the re-appearance period (RP) at an observation station, Ramp et al.(2004) divided the ISWs into two types:Type-a and Type-b. Type-a ISWs arrive regularly at the same time every day, i.e., the RP is about 24 h, and Type-b ISWs arrive about one hour late every day, i.e., the RP is about 25 h.展开更多
The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base edit...The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals.展开更多
The discovery of new-type ore deposits, an important approach to guarantee the mineral resources supply in the 21st century, often brings about a gigantic increase in the mineral resources reserves. The analysis of mi...The discovery of new-type ore deposits, an important approach to guarantee the mineral resources supply in the 21st century, often brings about a gigantic increase in the mineral resources reserves. The analysis of mineralization system is of great importance to the discovery of new type ore deposits. ① The understanding of the relationship among various ore deposit types within a mineralization system in a region can help us to locate the unknown ore deposit types from the known ore deposit types, evidence that has been proved in the mineral prospecting history of ore belts in the middle and lower reaches of the Yangtze River, China. ② The understanding of the spatial structure of a mineralization system, especially of the vertical zonation, is helpful for the discovery of the concealed ore deposit types. ③ Clarifying the temporal structure of a mineralization system, including the iteration relationship between the mineral deposit types in the mineralization, leads to the location of the missing mineralization chains from the known mineralization chains (mineral deposit type), a method often proved to be effective in the magmatic hydrothermal mineralization system.④ Clarifying the factors restraining the diversity of mineral deposit types in mineralization system leads to the discovery of the potential of new type mineral deposits in relevant region. ⑤ Studying new mineralization setting and new ore forming processes leads to discovery of new type mineral deposit. More probabilities of discovery of new type mineral deposits are present in biogenic mineralization system, deep sea mineralization system, low temperature mineralization system, tectonic mineralization system and superimposed mineralization system.展开更多
1-substituted-5-benzoylamino-4-pyrazolecarboxamides(2)were prepared by the reaction of 1-substituted pyrazolo[3,4-d]-oxazinone(1) with amines in benzene.The compound 2 was refluxed with excess LiAlH_4 in a mixture of ...1-substituted-5-benzoylamino-4-pyrazolecarboxamides(2)were prepared by the reaction of 1-substituted pyrazolo[3,4-d]-oxazinone(1) with amines in benzene.The compound 2 was refluxed with excess LiAlH_4 in a mixture of THF and ether,one of the two carbamoyl groups in the molecule 2 was reduced selectively.As the results,1-substituted-5- benzylamino-4-pyrazolecarboxamides(3)were obtained.Compounds 3 reacted with Cl_2PPh-X,or P(NET_2)_3,producing the title compounds(4). The conditions of the reactions were studied.展开更多
Recently Yang,Chu,and Han [Phys.Rev.A 70 (2004) 022329] presented a new type of multipartiteentangled states for implementing efficient many-party controlled teleportation of multiqubit quantum information.Herewe prop...Recently Yang,Chu,and Han [Phys.Rev.A 70 (2004) 022329] presented a new type of multipartiteentangled states for implementing efficient many-party controlled teleportation of multiqubit quantum information.Herewe propose a simple scheme for preparing such a type of multi-atom entangled states in cavity quantum electrodynamics(QED).The scheme involves atom-cavity interaction with large detuning,and is immune to the cavity decay and thethermal field states.Some practical analyses show its availability with the present technology.展开更多
A new type of conserved quantity, which is induced from the Mei symmetry of Lagrange systems, is studied. The conditions for that the new type of conserved quantity exists and the form of the new type of conserved qua...A new type of conserved quantity, which is induced from the Mei symmetry of Lagrange systems, is studied. The conditions for that the new type of conserved quantity exists and the form of the new type of conserved quantity are obtained. An illustrated example is given. The Noether conserved quantity induced from the Mei symmetry of Lagrange systems is a special case of the new type of conserved quantity given in this paper.展开更多
Under the direction of theory of human resources development,this thesis analyzes the impact of rural human resources development oncultivating new-type farmers.Firstly,it increases the input of rural basic education;...Under the direction of theory of human resources development,this thesis analyzes the impact of rural human resources development oncultivating new-type farmers.Firstly,it increases the input of rural basic education;secondly,it reinforces the vocational education and technologytraining;thirdly,it promotes the rural medical and public health services;fourthly,it quickens the rural labor transfer.The status quo of China's ruralhuman resources has been analyzed as follows:in terms of the quantity of rural human resources,the status quo is large and quick-developed baseof rural human resources,high labor participatory rate,and constitution of low age;in terms of the quality of rural human resources,the status quois the ubiquitous low quality of rural human resources,low technological level of rural human resources,and overall low physical quality of farmers;in terms of the structure of rural human resources,the status quo is the irrational industrial structure distribution and imbalanced regional structuredistribution.The thesis also discusses the edification of theory of human resources development in cultivating new-type farmers.First,in terms ofthe control over quantity of rural human resources,it is to keep the stability of family planning policy,and expedite the transfer of rural surplus la-bor;second,in terms of promoting the quality of rural human resources,it is to bolster the development of reserve rural labor force resources,toconstruct the adult educational training system with Chinese characteristics,and to build rural primary health care system;third,in terms of adjus-ting the structure of rural human resources,it is to perfect rural human resources market,and adjust rural economical structure and talents struc-ture.展开更多
We attempt to recover the paleocnvironments recorded in the accretion of a typical newtype hydrogenetic ferromanganese crust from the deep water areas of the East Philippine Sea. From detailed geochemical and U-series...We attempt to recover the paleocnvironments recorded in the accretion of a typical newtype hydrogenetic ferromanganese crust from the deep water areas of the East Philippine Sea. From detailed geochemical and U-series chronological studies, analysis of major and minor elements performed by X-ray fluorescence spectrometry (XRF) and inductively coupled plasma-mass spectrometer (ICPMS), three major accretion periods and corresponding paleocnvironments can be ascertained. The first period is a faster accretion period in the terminal Late Miocene to the Early Pliocene with looser structure and higher volcanic detritus content, corresponding to the active Antarctic bottom waters and depressed temperature from the intermediate Middle Miocene to the Early Pliocene. The second period is a pulse of pelagic clay deposition at the Early to Middle Pliocene, reflecting the shrinkage of the Antarctic bottom waters and the global temperature elevation of this period. The third period is a slower accretion period from the Middle Pliocene, which indicates the more violent activity of Antarctic bottom waters once again and more depressed temperature than the first period, facilitating the accretion of a more compact and pure ferromanganese zone. The paleoceanographic histories of these studied areas had not been made clear in previous research.展开更多
Objective: To replace the peripheral venous puncture for blood sampling with taking blood samples extracorporally from arterial line before predilution during RCA-CRRT performed. Methods: A new type of double-lumen fe...Objective: To replace the peripheral venous puncture for blood sampling with taking blood samples extracorporally from arterial line before predilution during RCA-CRRT performed. Methods: A new type of double-lumen femoral catheter was used instead of the current tubes. The new type of double-lumen tube had a greater distance from the inner venous ports to the inner arterial ports than current tubes. The minimum distance from the venous port to the arterial port was greatly lengthened. Replacement solution contained citrate, zero Ca<sup>2+</sup>, zero bicarbonate, low Na<sup>+</sup>. Blood samples were synchronously collected from the arterial line before the infusion of citrate replacement fluid and from the peripheral vein. The iCa concentration data of two groups were analyzed to observe the difference between iCa concentration levels in the arterial line and in peripheral vein;the anticoagulant effect of RCA and possible complications were observed, such as bleeding, clottings and hypocalcaemia. Results: 28 times of RCA-CRRT were performed on17 AKI and CRF patients with active bleeding or at the high risk of bleeding;336 blood samples were collected. Statistics showed that the difference of iCa concentration between arterial line group and the peripheral vein group was not significant (P = 0.9), there is a high degree of similarity between the iCa concentration of arterial line blood and the peripheral venous blood. None of the patients developed citrate toxicity or metabolic alkalosis. None induced bleeding, or bleeding aggravated. No obvious clotting occurred. Systemic calcium concentration was achieved in the ideal range. Conclusion: In clinical practice, the data of iCa concentration from arterial line can be used to replace that from peripheral vein when the new type of double-lumen femoral catheter is placed in femoral vein. RCA-CRRT therapy is safe and effective.展开更多
Based on the basic formula of the confidence interval and the sampling error of mathematical statistics, the mathematical statistics method of evaluating application effects of a new type of gas anchor was given in th...Based on the basic formula of the confidence interval and the sampling error of mathematical statistics, the mathematical statistics method of evaluating application effects of a new type of gas anchor was given in this paper. By the method mentioned above, the confidence interval and the sampling errors of the relevant mean value differences of Daqing Oilfield S block’s 150 wells, according to the mean value differences of the liquid producing capacity per day, the oil production per day, the submergence depth of the 10 sampling test wells, in which before and after a new type of gas anchor were laid down, were calculated. The calculation results show that a new type of gas anchor has a better effect of increasing oil production of oil well and enhancing pump efficiency. Through the real value differences analysis of the liquid producing capacity per day, the oil production per day, the submergence depth of 150 wells mentioned above, in which before and after a new type of gas anchor were laid down, it was verified. By using the confidence interval and the sampling errors of the liquid producing capacity per day, the oil production per day, the submergence depth mentioned above, in which before and after a new type of gas anchor were laid down, the application effects of a new type of gas anchor could be evaluated. And a mathematical statistics method of evaluation application effects of a new type of gas anchor is presented.展开更多
To enhance the yield potential of an elite indica rice cultivar,an introgression(BC_3-derived) line of IR64,YTH288,was developed using a new-plant-type cultivar,IR66215-44-2-3,as a donor parent.YTH288 has agronomicall...To enhance the yield potential of an elite indica rice cultivar,an introgression(BC_3-derived) line of IR64,YTH288,was developed using a new-plant-type cultivar,IR66215-44-2-3,as a donor parent.YTH288 has agronomically valuable characteristics such as large panicles,few unproductive tillers,and large leaves inherited from NPT.To identify the genetic basis of these traits,we used 167 F_2 plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits:days to heading(DTH),culm length(CL),flag leaf length(FLL),flag leaf width(FLW),and filled spikelet number per panicle(FSN).Six putative QTL were detected:four on chromosome4(for CL,FLL,FLW,and FSN) and two on chromosome 2(for DTH and FLL).All QTL with the IR66215-44-2-3 allele,except that for FLL on chromosome 2,had positive effects on each trait.To confirm the effects of these putative QTL,we developed NILs with the IR64 genetic background by marker-assisted selection.We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes2 and 4.Additionally,four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropic To enhance the yield potential of an elite indica rice cultivar, an introgression(BC3-derived) line of IR64, YTH288, was developed using a new-plant-type cultivar,IR66215-44-2-3, as a donor parent. YTH288 has agronomically valuable characteristics such as large panicles, few unproductive tillers, and large leaves inherited from NPT.To identify the genetic basis of these traits, we used 167 F2 plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits: days to heading(DTH), culm length(CL), flag leaf length(FLL), flag leaf width(FLW), and filled spikelet number per panicle(FSN). Six putative QTL were detected: four on chromosome4(for CL, FLL, FLW, and FSN) and two on chromosome 2(for DTH and FLL). All QTL with the IR66215-44-2-3 allele, except that for FLL on chromosome 2, had positive effects on each trait. To confirm the effects of these putative QTL, we developed NILs with the IR64 genetic background by marker-assisted selection. We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes2 and 4. Additionally, four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropiceffects for unique agronomic traits. These NILs can be used as research materials for studying each trait and as breeding materials for yield improvement of indica rice cultivars.展开更多
A renovation project of miniaturization and high efficiency is provided for the hot blast stove .The experimental data tested feasibility of the new-type swirl flow hot blast stove. The normal and hot state experiment...A renovation project of miniaturization and high efficiency is provided for the hot blast stove .The experimental data tested feasibility of the new-type swirl flow hot blast stove. The normal and hot state experiments have been done through changing the angle of gas entering into the regenerator. Factors influencing pressure drop have been studied and analyzed. The experimental results can be formulated in the form of the Ergun equation. The regression equation is obtained. And two modified coefficients are offered to the regenerator pressure drop of the new-type swirl flow hot blast stove.展开更多
With the continuous progress of new-type urbanization in Karst mountain area, how to correctly deal with the relationship between urban economy and land use are attracting much attention. Based on the land-use change ...With the continuous progress of new-type urbanization in Karst mountain area, how to correctly deal with the relationship between urban economy and land use are attracting much attention. Based on the land-use change data, this paper analyzes the characteristics of land use change and its driving factors in Guiyang with the method of land-use dynamic analysis, land utilization and factor correlation analysis. We get the following results:(1) the change of land use was obvious during 2004 and 2012. The area of arable land and other land were decreased, the rate of decrease are 3.89% and 8.24%. In the meantime, a series of construction land and forest area increased a lot, the rate of increase are 3.89% and 8.24%.(2) The integrated land use dynamic degree of two research stages(2004-2009 and 2009-2012) were decreased from 2.12% to 0.34%, this suggests dynamic changes of land tend to be stable.(3) Four kinds of Land use class have a great contribution to the index of land use degree respectively. The composite index of land use degree rose from 229.58 to 247.19 during 2004 and 2012; the land use is still in the development stage.(4) Economy, population, transportation and policy guidance are the main drivers of land use change.展开更多
文摘The Congolese population is organised into households, which are thus headed by a chief who ensures the social well-being, development and integration into working life of the individuals in his charge. This study examines the functional principles of new housing design as an instrument for transforming the (current) failing economy into a strong and resilient one. Accordingly, a literature review of the practice of designing and building housing in human settlements in the Congo revealed the state of the art on this subject. An analysis of the existing housing stock from a demographic, social and economic point of view made it possible to identify the most common household sizes and numbers, as well as the lifestyle processes that determine the need for developed space. To this end, the experimental method was used to propose configuration plans for various new types of dwelling. To this end, the study highlighted the link between people’s standard of living and the effectiveness of their involvement in the local economy. To achieve the aims of the National Development Plan (NDP), particular attention must be paid to solving the housing problem. The existing housing stock actively contributes to the problems associated with unemployment and insecurity. Functional principles for the design of new types of housing have been developed. Four model types are proposed in line with the demographic structure of the population, their socio-economic characteristics and their lifestyle.
基金The National Natural Science Foundation of China, No. 39970561The Key Projects in the National Science and Technology Pillar Program, 2007Z06-017+2 种基金Program for New Century Excellent Talents from Universities, Chinese Ministry of Education, No. NCET-04-0906/NCET-06-0818Fund of the Discipline Leaders of Sichuan Province, No. SZD0418Culture Fund for Excellent Doctoral Dissertations of Sichuan Agricultural University, 2008scybpy-1
文摘AIM: To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells. METHODS: The apoptosis of duck embryo fibroblasts (DEF) induced by NGVEV was investigated by fluorescence-activated cell sorter (FACS) and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide (PI). RESULTS: By staining cells with a combination of fluorescein annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells (Annexin V-FITC negative/PI negative), early apoptotic cells (Annexin V-FITC positive/PI negative), late apoptotic/necrotic cells (Annexin V-FITC positive/ PI positive) and dead cells (Annexin V-FITC negative/PI positive) through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non- apoptotic cells decreased.
基金the National Natural Science Foundation of China(Grant 40773038)Geological Exploration Project Office of Inner Mongolia (05-1-TK01)the Institute of Mineral Resources, Chinese Academy of Geological Sciences(K0714)
文摘Anatase and its allomorphic mineral rutile have the most prominent economic significance among titanium mineral resources and constitute one of the badly needed mineral resources currently in China. The Yantizishan-Moshishan anatase deposit was formerly referred to as an iron deposit. Based on recent investigation and exploration the authors believe that it is actually a large metamorphosed sedimentary anatase-dominated deposit belonging to a new genetic type. Ore bodies occur in stratoid and lenticular forms in Mesoproterozoic (1751 Ma) schist, metasandstone (metasiltstone), and amphibolite. Rich ores have perthitic structure comprising chiefly interbedded quartz perthite (with disseminated anatase and rutile) and anatase perthite. Ore minerals are mainly anatase and subordinately rutile and ilmenite (±hematite), while nonmetallic minerals are chiefly quartz with a certain amount of anthophyllite and biotite (±garnet). The grain sizes of anatase, ruffle and ilmenite are 0.01-0.1 mm. Rich ores contain 3.14% to 15.46% TiO2. averaging 6.91%, while the low-grade ores have TiO2 content about 1.2%to 2.97%, averaging 1.76%. The ores have relatively high TFe and V contents. Trace elements in anatase and rutile such as Nb and Cr were analyzed by the electron microprobe. According to their relatively low Nb and Cr contents, source anatase and rutile must have come from meta-mafic rocks. Trace elements of the associated ilmenite show relatively high MnO and low MgO contents, just in contrast to those of ilmenite in V-Ti-magnetite ores of magmatic origin. The protoliths of amphibolite wall rocks should be basalt and picrite-basalt. Pertochemical data suggest that the tectonic setting of these rocks belongs to an island arc or a transitional belt between the island arc and oceanic ridge. Silicon isotope study shows that δ30Si values of different anatase ores, quartzite, and schist in this deposit are 0.1‰ to -0.9‰, similar to those of marine hydrothermal exhalative sedimentary deposits. All of these geological and geochemical characteristics of the ore deposit suggest that the anatase ores and amphibolite are products of submarine basic volcanism. The ores had chemical precipitation features, but were later subjected to regional intermediate (or somewhat lower) grade metamorphism (1158 Ma). Rutile was formed mainly in the process of this metamorphism. The ore belt locally underwent hydrothermal modification during the emplacement of Late Yanshanian granite (118 Ma).
文摘Both new plant type (NPT) and intersubspecific hybrid rice (IHR) had large sink size (total number of spikelets per m2), however, poor grain filling limited their potential in the grain yield. Compared to the three-line indica hybrid of Shanyou 63 (CK), NPT and IHR showed higher photosynthetic potential, higher dry matter accumulation and higher ratio of dry weight to spikelets (total dry wt./total number of spikelets) from heading to harvest. But both exhibited a low export percentage and transfer ratio of assimilates, low partitioning of 14C to grains from labeled flag leaves, low harvest indices and low physiological activities (IAA content and activities of ATPase and starch synthase) of grains at early grain-filling stage. The physiological activities of grains at early filling stage were significantly correlated with the export percentage and transfer ratio of assimilates, ripened-grain percentage and grain plumpness (r = 0.85 - 0.95). The source-sink ratio (dry matter wt./spikelet and nonstructural carbohydrate/spikelet) at heading was positively correlated with physiological activities of grains (r = 0. 84 - 0. 97 ). It is suggested that low physiological activities of grains at early filling stage is attributed to low source-sink ratio at heading, and the low sink activity weakens the ability to remobilize assimilates into grains, and leads to poor grain filling in NPT and IHR.
文摘In this paper, a photoelectric signal obtained scheme via equator triangle pattern engraved on rotor is discussed and the mathematic model is deduced in the case which is deflexion between rotor axis and the coordinate frame of case. The deflexion error and coupling error under the situation are analyzed. Finally, three methods of engraving based on the spherical triangle pattern are presented. The error models of various methods are built up and the simulation curves are provided respectively. We have done the primary experiments on the surface of rotor using this method. It can be seen from the enlarged figures that the rim of the pattern is smooth and the demand of sensor resolution is satisfied by and large. The results of study supply reference for signal obtaining.
基金provided by the Office of National Science and Technology (No.2006BAB16B01)the Physics Program of the Henan Province Education Department (No.2009B 560004)the Doctoral Fund of Henan Polytechnic University (No.648234)
文摘Numerical simulation was used to estimate the temperature field within a poured concrete,mono-layer freezing-shaft lining.The affects from various factors were investigated.The maximum temperature within the lining increases as the lining thickness increases,decreases as the soil-side wall temperature decreases,decreases as the air temperature inside the shaft decreases and decreases as the air velocity inside the shaft increases.The compression speed of an insulating foam layer affects the maximum temperature difference between the interior and the sidewalls.The maximum temperature difference between the interior and the sidewalls approaches or exceeds the maximum allowable for the curing of poured concrete structures.Attention should be paid to the question of the lining cracking during the curing period.The temperature gradient in the vertical direction may be minimized by preventing air contact against the steel connection board supporting the base of the freshly poured section.
基金The National Science and Technology Major Project of China under contract No.2016ZX05057015the National Natural Science Foundation of China under contract Nos 41376038 and 40406009+3 种基金the NSFC-Shandong Joint Fund for Marine Science Research Centers of China under contract No.U1606405the National Program on Global Change and Air-Sea Interaction of China under contract Nos GASI-03-01-01-02,GASI-02-IND-STSsum and GASI-IPOVAI-01-05the Public Science and Technology Research Funds Projects of Ocean of China under contract No.200905024the National Key Scientific Instrument and Equipment Development Projects of China under contract No.2012YQ12003908
文摘The South China Sea (SCS), in particular the northern SCS, is one of ocean areas where energetic internal solitary waves (ISWs)occur most frequently (Cai et al., 2012; Zheng, 2017). Based on the re-appearance period (RP) at an observation station, Ramp et al.(2004) divided the ISWs into two types:Type-a and Type-b. Type-a ISWs arrive regularly at the same time every day, i.e., the RP is about 24 h, and Type-b ISWs arrive about one hour late every day, i.e., the RP is about 25 h.
基金supported by the Beijing Scholars Program[BSP041]。
文摘The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals.
文摘The discovery of new-type ore deposits, an important approach to guarantee the mineral resources supply in the 21st century, often brings about a gigantic increase in the mineral resources reserves. The analysis of mineralization system is of great importance to the discovery of new type ore deposits. ① The understanding of the relationship among various ore deposit types within a mineralization system in a region can help us to locate the unknown ore deposit types from the known ore deposit types, evidence that has been proved in the mineral prospecting history of ore belts in the middle and lower reaches of the Yangtze River, China. ② The understanding of the spatial structure of a mineralization system, especially of the vertical zonation, is helpful for the discovery of the concealed ore deposit types. ③ Clarifying the temporal structure of a mineralization system, including the iteration relationship between the mineral deposit types in the mineralization, leads to the location of the missing mineralization chains from the known mineralization chains (mineral deposit type), a method often proved to be effective in the magmatic hydrothermal mineralization system.④ Clarifying the factors restraining the diversity of mineral deposit types in mineralization system leads to the discovery of the potential of new type mineral deposits in relevant region. ⑤ Studying new mineralization setting and new ore forming processes leads to discovery of new type mineral deposit. More probabilities of discovery of new type mineral deposits are present in biogenic mineralization system, deep sea mineralization system, low temperature mineralization system, tectonic mineralization system and superimposed mineralization system.
基金Project supported by National Natural Science Foundation of China.
文摘1-substituted-5-benzoylamino-4-pyrazolecarboxamides(2)were prepared by the reaction of 1-substituted pyrazolo[3,4-d]-oxazinone(1) with amines in benzene.The compound 2 was refluxed with excess LiAlH_4 in a mixture of THF and ether,one of the two carbamoyl groups in the molecule 2 was reduced selectively.As the results,1-substituted-5- benzylamino-4-pyrazolecarboxamides(3)were obtained.Compounds 3 reacted with Cl_2PPh-X,or P(NET_2)_3,producing the title compounds(4). The conditions of the reactions were studied.
基金the Natural Science Foundation of Hunan Province under Grant No.06JJ5015the Scientific Research Fund of the Education Department of Hunan Province under Grant No.06C354
文摘Recently Yang,Chu,and Han [Phys.Rev.A 70 (2004) 022329] presented a new type of multipartiteentangled states for implementing efficient many-party controlled teleportation of multiqubit quantum information.Herewe propose a simple scheme for preparing such a type of multi-atom entangled states in cavity quantum electrodynamics(QED).The scheme involves atom-cavity interaction with large detuning,and is immune to the cavity decay and thethermal field states.Some practical analyses show its availability with the present technology.
文摘A new type of conserved quantity, which is induced from the Mei symmetry of Lagrange systems, is studied. The conditions for that the new type of conserved quantity exists and the form of the new type of conserved quantity are obtained. An illustrated example is given. The Noether conserved quantity induced from the Mei symmetry of Lagrange systems is a special case of the new type of conserved quantity given in this paper.
文摘Under the direction of theory of human resources development,this thesis analyzes the impact of rural human resources development oncultivating new-type farmers.Firstly,it increases the input of rural basic education;secondly,it reinforces the vocational education and technologytraining;thirdly,it promotes the rural medical and public health services;fourthly,it quickens the rural labor transfer.The status quo of China's ruralhuman resources has been analyzed as follows:in terms of the quantity of rural human resources,the status quo is large and quick-developed baseof rural human resources,high labor participatory rate,and constitution of low age;in terms of the quality of rural human resources,the status quois the ubiquitous low quality of rural human resources,low technological level of rural human resources,and overall low physical quality of farmers;in terms of the structure of rural human resources,the status quo is the irrational industrial structure distribution and imbalanced regional structuredistribution.The thesis also discusses the edification of theory of human resources development in cultivating new-type farmers.First,in terms ofthe control over quantity of rural human resources,it is to keep the stability of family planning policy,and expedite the transfer of rural surplus la-bor;second,in terms of promoting the quality of rural human resources,it is to bolster the development of reserve rural labor force resources,toconstruct the adult educational training system with Chinese characteristics,and to build rural primary health care system;third,in terms of adjus-ting the structure of rural human resources,it is to perfect rural human resources market,and adjust rural economical structure and talents struc-ture.
基金This paperis supported bythe Pilot Project of the Knowledge InnovationProgramof Chinese Academy of Sciences (No . KZCX3-SW-223) theNational Natural Science Foundation of China ( Nos . 40506016 and40576032) .
文摘We attempt to recover the paleocnvironments recorded in the accretion of a typical newtype hydrogenetic ferromanganese crust from the deep water areas of the East Philippine Sea. From detailed geochemical and U-series chronological studies, analysis of major and minor elements performed by X-ray fluorescence spectrometry (XRF) and inductively coupled plasma-mass spectrometer (ICPMS), three major accretion periods and corresponding paleocnvironments can be ascertained. The first period is a faster accretion period in the terminal Late Miocene to the Early Pliocene with looser structure and higher volcanic detritus content, corresponding to the active Antarctic bottom waters and depressed temperature from the intermediate Middle Miocene to the Early Pliocene. The second period is a pulse of pelagic clay deposition at the Early to Middle Pliocene, reflecting the shrinkage of the Antarctic bottom waters and the global temperature elevation of this period. The third period is a slower accretion period from the Middle Pliocene, which indicates the more violent activity of Antarctic bottom waters once again and more depressed temperature than the first period, facilitating the accretion of a more compact and pure ferromanganese zone. The paleoceanographic histories of these studied areas had not been made clear in previous research.
文摘Objective: To replace the peripheral venous puncture for blood sampling with taking blood samples extracorporally from arterial line before predilution during RCA-CRRT performed. Methods: A new type of double-lumen femoral catheter was used instead of the current tubes. The new type of double-lumen tube had a greater distance from the inner venous ports to the inner arterial ports than current tubes. The minimum distance from the venous port to the arterial port was greatly lengthened. Replacement solution contained citrate, zero Ca<sup>2+</sup>, zero bicarbonate, low Na<sup>+</sup>. Blood samples were synchronously collected from the arterial line before the infusion of citrate replacement fluid and from the peripheral vein. The iCa concentration data of two groups were analyzed to observe the difference between iCa concentration levels in the arterial line and in peripheral vein;the anticoagulant effect of RCA and possible complications were observed, such as bleeding, clottings and hypocalcaemia. Results: 28 times of RCA-CRRT were performed on17 AKI and CRF patients with active bleeding or at the high risk of bleeding;336 blood samples were collected. Statistics showed that the difference of iCa concentration between arterial line group and the peripheral vein group was not significant (P = 0.9), there is a high degree of similarity between the iCa concentration of arterial line blood and the peripheral venous blood. None of the patients developed citrate toxicity or metabolic alkalosis. None induced bleeding, or bleeding aggravated. No obvious clotting occurred. Systemic calcium concentration was achieved in the ideal range. Conclusion: In clinical practice, the data of iCa concentration from arterial line can be used to replace that from peripheral vein when the new type of double-lumen femoral catheter is placed in femoral vein. RCA-CRRT therapy is safe and effective.
文摘Based on the basic formula of the confidence interval and the sampling error of mathematical statistics, the mathematical statistics method of evaluating application effects of a new type of gas anchor was given in this paper. By the method mentioned above, the confidence interval and the sampling errors of the relevant mean value differences of Daqing Oilfield S block’s 150 wells, according to the mean value differences of the liquid producing capacity per day, the oil production per day, the submergence depth of the 10 sampling test wells, in which before and after a new type of gas anchor were laid down, were calculated. The calculation results show that a new type of gas anchor has a better effect of increasing oil production of oil well and enhancing pump efficiency. Through the real value differences analysis of the liquid producing capacity per day, the oil production per day, the submergence depth of 150 wells mentioned above, in which before and after a new type of gas anchor were laid down, it was verified. By using the confidence interval and the sampling errors of the liquid producing capacity per day, the oil production per day, the submergence depth mentioned above, in which before and after a new type of gas anchor were laid down, the application effects of a new type of gas anchor could be evaluated. And a mathematical statistics method of evaluation application effects of a new type of gas anchor is presented.
基金the results obtained from phases Ⅲ , Ⅳ, Ⅴ, and Ⅵ of the IRRI – Japan Collaborative Research Project, which was supported by the Ministry of Foreign Affairs and the Ministry of Agriculture, Forestry and Fisheries of Japan
文摘To enhance the yield potential of an elite indica rice cultivar,an introgression(BC_3-derived) line of IR64,YTH288,was developed using a new-plant-type cultivar,IR66215-44-2-3,as a donor parent.YTH288 has agronomically valuable characteristics such as large panicles,few unproductive tillers,and large leaves inherited from NPT.To identify the genetic basis of these traits,we used 167 F_2 plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits:days to heading(DTH),culm length(CL),flag leaf length(FLL),flag leaf width(FLW),and filled spikelet number per panicle(FSN).Six putative QTL were detected:four on chromosome4(for CL,FLL,FLW,and FSN) and two on chromosome 2(for DTH and FLL).All QTL with the IR66215-44-2-3 allele,except that for FLL on chromosome 2,had positive effects on each trait.To confirm the effects of these putative QTL,we developed NILs with the IR64 genetic background by marker-assisted selection.We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes2 and 4.Additionally,four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropic To enhance the yield potential of an elite indica rice cultivar, an introgression(BC3-derived) line of IR64, YTH288, was developed using a new-plant-type cultivar,IR66215-44-2-3, as a donor parent. YTH288 has agronomically valuable characteristics such as large panicles, few unproductive tillers, and large leaves inherited from NPT.To identify the genetic basis of these traits, we used 167 F2 plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits: days to heading(DTH), culm length(CL), flag leaf length(FLL), flag leaf width(FLW), and filled spikelet number per panicle(FSN). Six putative QTL were detected: four on chromosome4(for CL, FLL, FLW, and FSN) and two on chromosome 2(for DTH and FLL). All QTL with the IR66215-44-2-3 allele, except that for FLL on chromosome 2, had positive effects on each trait. To confirm the effects of these putative QTL, we developed NILs with the IR64 genetic background by marker-assisted selection. We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes2 and 4. Additionally, four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropiceffects for unique agronomic traits. These NILs can be used as research materials for studying each trait and as breeding materials for yield improvement of indica rice cultivars.
文摘A renovation project of miniaturization and high efficiency is provided for the hot blast stove .The experimental data tested feasibility of the new-type swirl flow hot blast stove. The normal and hot state experiments have been done through changing the angle of gas entering into the regenerator. Factors influencing pressure drop have been studied and analyzed. The experimental results can be formulated in the form of the Ergun equation. The regression equation is obtained. And two modified coefficients are offered to the regenerator pressure drop of the new-type swirl flow hot blast stove.
文摘With the continuous progress of new-type urbanization in Karst mountain area, how to correctly deal with the relationship between urban economy and land use are attracting much attention. Based on the land-use change data, this paper analyzes the characteristics of land use change and its driving factors in Guiyang with the method of land-use dynamic analysis, land utilization and factor correlation analysis. We get the following results:(1) the change of land use was obvious during 2004 and 2012. The area of arable land and other land were decreased, the rate of decrease are 3.89% and 8.24%. In the meantime, a series of construction land and forest area increased a lot, the rate of increase are 3.89% and 8.24%.(2) The integrated land use dynamic degree of two research stages(2004-2009 and 2009-2012) were decreased from 2.12% to 0.34%, this suggests dynamic changes of land tend to be stable.(3) Four kinds of Land use class have a great contribution to the index of land use degree respectively. The composite index of land use degree rose from 229.58 to 247.19 during 2004 and 2012; the land use is still in the development stage.(4) Economy, population, transportation and policy guidance are the main drivers of land use change.