In January of 2008 a rare calamity occurred, i.e., a wide-spread snow storm hit southeast and southwes provinces of China. Tens thousands of transmission lines were interrupted, and tens thousands of towers frosted up...In January of 2008 a rare calamity occurred, i.e., a wide-spread snow storm hit southeast and southwes provinces of China. Tens thousands of transmission lines were interrupted, and tens thousands of towers frosted up and collapsed. Traffic stopped. Blackout occurred in many regions. 14 workers died in accidents while repairing the power transmission facilities. All Chinese, including the top leaders of CPC and Chinese government, were worried about the victims o the disaster. Obviously, it is not enough to think about only improving the power line design standards and other tech nological measures. A structural and systematic analysis shall be made at a level of the core value of electric industry.展开更多
The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base edit...The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals.展开更多
The rapid development of information technology and the fierce conflict of multiple values lead information communication to present different characteristics in the new media era,which has brought both challenges and...The rapid development of information technology and the fierce conflict of multiple values lead information communication to present different characteristics in the new media era,which has brought both challenges and opportunities to the cultivation and dissemination of core values.With the development of new media,the environment for cultivating core values is becoming increasingly complex,and the main content of core values is being dispelled,thus weakening its function of leading and affecting its guiding role.At the same time,the development of new media can not only broaden the cultivation mode of core values and highlight the powerful force of core values,but also stimulate the people to highly identify with core values.At present,the new media has become the main tool and mainstream position for the dissemination of core values,so it is necessary to continuously innovate the communication mechanism of the core values,and enhance their pertinence and effectiveness communication,so that they can play a leading role in the whole society better.展开更多
New media is not only an emerging media and a unique cultural form,but also a mechanismproducing,expressing and disseminating ideology such as the socialist core values.In the process of cultivating college students’...New media is not only an emerging media and a unique cultural form,but also a mechanismproducing,expressing and disseminating ideology such as the socialist core values.In the process of cultivating college students’core values,characteristics of new media such as information production,expression and propagation should be deeply understood and exploited.Accordingly,college students’positive values will be fully guided by new media,thus cultivating and shaping a new generation firmly fulfilling the socialist core values in China.展开更多
文摘In January of 2008 a rare calamity occurred, i.e., a wide-spread snow storm hit southeast and southwes provinces of China. Tens thousands of transmission lines were interrupted, and tens thousands of towers frosted up and collapsed. Traffic stopped. Blackout occurred in many regions. 14 workers died in accidents while repairing the power transmission facilities. All Chinese, including the top leaders of CPC and Chinese government, were worried about the victims o the disaster. Obviously, it is not enough to think about only improving the power line design standards and other tech nological measures. A structural and systematic analysis shall be made at a level of the core value of electric industry.
基金supported by the Beijing Scholars Program[BSP041]。
文摘The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals.
基金sponsored by the 2016 Key grant of Anhui Humanity and Social Science(No.SK2016A0508)
文摘The rapid development of information technology and the fierce conflict of multiple values lead information communication to present different characteristics in the new media era,which has brought both challenges and opportunities to the cultivation and dissemination of core values.With the development of new media,the environment for cultivating core values is becoming increasingly complex,and the main content of core values is being dispelled,thus weakening its function of leading and affecting its guiding role.At the same time,the development of new media can not only broaden the cultivation mode of core values and highlight the powerful force of core values,but also stimulate the people to highly identify with core values.At present,the new media has become the main tool and mainstream position for the dissemination of core values,so it is necessary to continuously innovate the communication mechanism of the core values,and enhance their pertinence and effectiveness communication,so that they can play a leading role in the whole society better.
基金funded by The historical materialism innovation research on General Party Secretary Xi Jingping’s important speech series,Major National Social Scientific fund project(142 DA004)The research on the realization of socialist core value in college cultural hegem ony based on mass data,the 13th Five-year Plan research project(moral special)of 2016 Guangdong Province Education Science(2016JKDY13)
文摘New media is not only an emerging media and a unique cultural form,but also a mechanismproducing,expressing and disseminating ideology such as the socialist core values.In the process of cultivating college students’core values,characteristics of new media such as information production,expression and propagation should be deeply understood and exploited.Accordingly,college students’positive values will be fully guided by new media,thus cultivating and shaping a new generation firmly fulfilling the socialist core values in China.
