The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base edit...The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals.展开更多
We are very pIeased to announce that five Associate Editors have joined the editorial team of the Joumal of Environmental Sciences （JES）. Professors/Drs. Yong Cai, Paul Lain, Jonathan Martin, Michael Plewa, and Po K...We are very pIeased to announce that five Associate Editors have joined the editorial team of the Joumal of Environmental Sciences （JES）. Professors/Drs. Yong Cai, Paul Lain, Jonathan Martin, Michael Plewa, and Po Keung Wong bring a wealth of expertise in environmental sciences. As preeminent scientists in their chosen areas of research.展开更多
The year 2012 is the one that carries far-reaching importance to China’s development course.Within the year,the Chinese people worked with concerted efforts,overcame obstacles and difficulties,and recorded outstandin...The year 2012 is the one that carries far-reaching importance to China’s development course.Within the year,the Chinese people worked with concerted efforts,overcame obstacles and difficulties,and recorded outstanding progress for the reform an opening-up as well as modernization,so that social and economic development presented a sound posture with some progress in the process of maintaining stability,and people’s livelihood was continuously improved.展开更多
基金supported by the Beijing Scholars Program[BSP041]。
文摘The clustered regularly interspaced short palindromic repeats(CRISPR)–CRISPR-associated protein(Cas) system has been widely used for genome editing. In this system, the cytosine base editor(CBE) and adenine base editor(ABE) allow generating precise and irreversible base mutations in a programmable manner and have been used in many different types of cells and organisms. However, their applications are limited by low editing efficiency at certain genomic target sites or at specific target cytosine(C) or adenine(A) residues. Using a strategy of combining optimized synergistic core components, we developed a new multiplex super-assembled ABE(sABE) in rice that showed higher base-editing efficiency than previously developed ABEs. We also designed a new type of nuclear localization signal(NLS) comprising a FLAG epitope tag with four copies of a codon-optimized NLS(F4NLS^(r2)) to generate another ABE named F4NLS-sABE. This new NLS increased editing efficiency or edited additional A at several target sites. A new multiplex super-assembled CBE(sCBE) and F4NLS^(r2) involved F4NLS-sCBE were also created using the same strategy. F4NLS-sCBE was proven to be much more efficient than sCBE in rice. These optimized base editors will serve as powerful genome-editing tools for basic research or molecular breeding in rice and will provide a reference for the development of superior editing tools for other plants or animals.
文摘We are very pIeased to announce that five Associate Editors have joined the editorial team of the Joumal of Environmental Sciences （JES）. Professors/Drs. Yong Cai, Paul Lain, Jonathan Martin, Michael Plewa, and Po Keung Wong bring a wealth of expertise in environmental sciences. As preeminent scientists in their chosen areas of research.
文摘The year 2012 is the one that carries far-reaching importance to China’s development course.Within the year,the Chinese people worked with concerted efforts,overcame obstacles and difficulties,and recorded outstanding progress for the reform an opening-up as well as modernization,so that social and economic development presented a sound posture with some progress in the process of maintaining stability,and people’s livelihood was continuously improved.
