A new dye-staining method for protein assay is described.The reaction between TPPS_4 and protein molecule causes a shift in the absorption of TPPS_4 from 435 nm to 488nm.The absorbance at 488 nm is proportional to the...A new dye-staining method for protein assay is described.The reaction between TPPS_4 and protein molecule causes a shift in the absorption of TPPS_4 from 435 nm to 488nm.The absorbance at 488 nm is proportional to the concentration of protein.The range of Beer's law was 1-5 ug/ml and the Sandell's sensitivity was 0.0087 ug/cm^2.展开更多
We describe a new method for angular displacement measurements that is based on a Fabry-Perot interferometer. A measurement accuracy of 10-8 rad is obtained by use of the sinusoidal phase modulating interferometry. An...We describe a new method for angular displacement measurements that is based on a Fabry-Perot interferometer. A measurement accuracy of 10-8 rad is obtained by use of the sinusoidal phase modulating interferometry. Another Fabry-Perot interferometer is used to obtain the key initial angle of incidence.展开更多
Studies in vitro show that intravascular ultrasound (IVUS) underestimates vessel and lumen dimensions. In order to validate IVUS measurement in vivo, we conducted a comparative study during catheterization in fifty pa...Studies in vitro show that intravascular ultrasound (IVUS) underestimates vessel and lumen dimensions. In order to validate IVUS measurement in vivo, we conducted a comparative study during catheterization in fifty patients. The patients underwent IVUS examinations for the purpose of diagnosis or treatment of coronary artery disease. The IVUS system was a 3.5 F, 20 MHz IVUS catheter (Sonicath catheter of Boston Scientific Co.) and a Hewlett Packard console. After examination of the coronary artery, the IVUS probe was withdrawn back into the guiding catheter to measure the average lumen diameter of the guiding catheter (8 F, Cordis). This measurement in vivo (VI) was compared with the true lumen diameter provided by the manufac Department of Cardiology, University of Essen, Essen, Germany (Liu FQ, Ge JB, Baumgart D, Haude M, Caspari G, Grge G, Eick B and Erbel R) turer (MA) and determined by on line quantitative angiography (HICOR, Siemens) (HI). In addition, the IVUS measurement in vitro (VT) was also taken with the same guiding catheter in waterbath at 37℃. The results showed that IVUS underestimated the true lumen diameter by 2.2%±2.6% in vivo, by 3.1%±1.8% in vitro, while HICOR owerestimated the true lumen diameter by 23.0%±6.8%. There was no difference between the IVUS measurements in vivo and in vitro. In summary, IVUS was very accurate for the measurement of a 8 F guiding catheter in vivo with only a minor underestimation, and IVUS measurement was far more reliable than the on line quantitative angiography.展开更多
In this paper, a new method is outlined for the estimation of coupling efficiency between a source laser and a WGPD. Internal quantum efficiencies as high as 72% (for 0.15μm device) and 86.5% (for 0.5μm device) are ...In this paper, a new method is outlined for the estimation of coupling efficiency between a source laser and a WGPD. Internal quantum efficiencies as high as 72% (for 0.15μm device) and 86.5% (for 0.5μm device) are achieved.展开更多
Using second-order autocorrelation conception, a novel method and instrument for accurately measuring interval between two linearly polarized ultrashort pulses with real time were presented. The experiment demonstrate...Using second-order autocorrelation conception, a novel method and instrument for accurately measuring interval between two linearly polarized ultrashort pulses with real time were presented. The experiment demonstrated that the measuring method and instrument were simple and accurate (the measurement error < 5 fs). During measuring, there was no moving element resulting in dynamic measurement error.展开更多
文摘A new dye-staining method for protein assay is described.The reaction between TPPS_4 and protein molecule causes a shift in the absorption of TPPS_4 from 435 nm to 488nm.The absorbance at 488 nm is proportional to the concentration of protein.The range of Beer's law was 1-5 ug/ml and the Sandell's sensitivity was 0.0087 ug/cm^2.
基金This work was supported by the National High Tech-nology Development 863 Program(2002aa404050)of China and the Foundation for Development of science of Shanghai(0114nm013),
文摘We describe a new method for angular displacement measurements that is based on a Fabry-Perot interferometer. A measurement accuracy of 10-8 rad is obtained by use of the sinusoidal phase modulating interferometry. Another Fabry-Perot interferometer is used to obtain the key initial angle of incidence.
文摘Studies in vitro show that intravascular ultrasound (IVUS) underestimates vessel and lumen dimensions. In order to validate IVUS measurement in vivo, we conducted a comparative study during catheterization in fifty patients. The patients underwent IVUS examinations for the purpose of diagnosis or treatment of coronary artery disease. The IVUS system was a 3.5 F, 20 MHz IVUS catheter (Sonicath catheter of Boston Scientific Co.) and a Hewlett Packard console. After examination of the coronary artery, the IVUS probe was withdrawn back into the guiding catheter to measure the average lumen diameter of the guiding catheter (8 F, Cordis). This measurement in vivo (VI) was compared with the true lumen diameter provided by the manufac Department of Cardiology, University of Essen, Essen, Germany (Liu FQ, Ge JB, Baumgart D, Haude M, Caspari G, Grge G, Eick B and Erbel R) turer (MA) and determined by on line quantitative angiography (HICOR, Siemens) (HI). In addition, the IVUS measurement in vitro (VT) was also taken with the same guiding catheter in waterbath at 37℃. The results showed that IVUS underestimated the true lumen diameter by 2.2%±2.6% in vivo, by 3.1%±1.8% in vitro, while HICOR owerestimated the true lumen diameter by 23.0%±6.8%. There was no difference between the IVUS measurements in vivo and in vitro. In summary, IVUS was very accurate for the measurement of a 8 F guiding catheter in vivo with only a minor underestimation, and IVUS measurement was far more reliable than the on line quantitative angiography.
文摘In this paper, a new method is outlined for the estimation of coupling efficiency between a source laser and a WGPD. Internal quantum efficiencies as high as 72% (for 0.15μm device) and 86.5% (for 0.5μm device) are achieved.
文摘Using second-order autocorrelation conception, a novel method and instrument for accurately measuring interval between two linearly polarized ultrashort pulses with real time were presented. The experiment demonstrated that the measuring method and instrument were simple and accurate (the measurement error < 5 fs). During measuring, there was no moving element resulting in dynamic measurement error.
