Recycling is an alternative for preserving historical heritage buildings. Through such process, buildings are occupied by new usages that assure they are not abandoned. However, conservation issues, like pathological ...Recycling is an alternative for preserving historical heritage buildings. Through such process, buildings are occupied by new usages that assure they are not abandoned. However, conservation issues, like pathological manifestations by filamentous fungi, are common even in buildings receiving new uses. Thus, it is extremely important to know the microclimate behavior of these buildings and analyze if there are usages that favor the appearance of filamentous fungi or not. This study aims to verify if new usages modify the microclimate in historical buildings and if such possible change favors the appearance of pathological manifestations by filamentous fimgi in some specific use. To achieve the results, three buildings that have gone through recycling processes were chosen in the city of Pelotas, in Southernmost Brazil. The city possesses a large number of buildings with historical value and its climate conditions are propitious for appearance of filamentous fungi. For 12 months, microclimate was monitored, the environments modus operandi was characterized and there was a survey on the pathological manifestations by filamentous fungi in the three buildings with two different uses： administration offices and exhibition rooms. Conclusion was that usages that allow natural ventilation and lighting favor conservation, which is what happens in the ones used as administration offices. Besides that, it was noticed that the construction system also influences, since buildings with a high cellar that is being used did not present filamentous fungi, which also shows the influence of ascending humidity on the appearance of such pathological manifestations.展开更多
In recent years, tumor-nfiltrating lymphocytes (TILs) have been reported to be effective for tumors in experimental and clinical research. In order to increase the therapeutical effect, we modified some steps of Rosen...In recent years, tumor-nfiltrating lymphocytes (TILs) have been reported to be effective for tumors in experimental and clinical research. In order to increase the therapeutical effect, we modified some steps of Rosenberg's approach a. cold digestion with collagenase at 4C for 24 hours; b. sedimentation instead of centrifugation; c. elimination of tumor cells before the cultivation procedure. Compared with the original approach, the proliferation, activity and cytotoxicity of TILs obtained by the modified procedure were much improved. TILs' expansion-old was greater than that with the original approach. Cytotoxicity against rumor cells was more potent. Increased TILs' subsets were CD3 and CD8 cells. Meanwhile, we took tumor cells from tumor tissues to test their in vitro chemosensitivities to different drugs in order to select highly sensitive antitumor drugs for treatment of cases with advanced tumors. According to the design of using highly active TILs and highly sensitive drugs (H & H therapy), preliminary clinical results of 50 cases showed higher response rates than those in treatment with TIL / IL2, LAK / 1L2 and TIL+IL2+CTX. Less toxic side effects were observed in 14 patients.展开更多
文摘Recycling is an alternative for preserving historical heritage buildings. Through such process, buildings are occupied by new usages that assure they are not abandoned. However, conservation issues, like pathological manifestations by filamentous fungi, are common even in buildings receiving new uses. Thus, it is extremely important to know the microclimate behavior of these buildings and analyze if there are usages that favor the appearance of filamentous fungi or not. This study aims to verify if new usages modify the microclimate in historical buildings and if such possible change favors the appearance of pathological manifestations by filamentous fimgi in some specific use. To achieve the results, three buildings that have gone through recycling processes were chosen in the city of Pelotas, in Southernmost Brazil. The city possesses a large number of buildings with historical value and its climate conditions are propitious for appearance of filamentous fungi. For 12 months, microclimate was monitored, the environments modus operandi was characterized and there was a survey on the pathological manifestations by filamentous fungi in the three buildings with two different uses： administration offices and exhibition rooms. Conclusion was that usages that allow natural ventilation and lighting favor conservation, which is what happens in the ones used as administration offices. Besides that, it was noticed that the construction system also influences, since buildings with a high cellar that is being used did not present filamentous fungi, which also shows the influence of ascending humidity on the appearance of such pathological manifestations.
文摘In recent years, tumor-nfiltrating lymphocytes (TILs) have been reported to be effective for tumors in experimental and clinical research. In order to increase the therapeutical effect, we modified some steps of Rosenberg's approach a. cold digestion with collagenase at 4C for 24 hours; b. sedimentation instead of centrifugation; c. elimination of tumor cells before the cultivation procedure. Compared with the original approach, the proliferation, activity and cytotoxicity of TILs obtained by the modified procedure were much improved. TILs' expansion-old was greater than that with the original approach. Cytotoxicity against rumor cells was more potent. Increased TILs' subsets were CD3 and CD8 cells. Meanwhile, we took tumor cells from tumor tissues to test their in vitro chemosensitivities to different drugs in order to select highly sensitive antitumor drugs for treatment of cases with advanced tumors. According to the design of using highly active TILs and highly sensitive drugs (H & H therapy), preliminary clinical results of 50 cases showed higher response rates than those in treatment with TIL / IL2, LAK / 1L2 and TIL+IL2+CTX. Less toxic side effects were observed in 14 patients.
