Abnormal Expression of Eukaryotic Translation Factors in Malignant Transformed Human Bronchial Epithelial Cells Induced by Crystalline Nickel Sulfide

Objective To study the oncogenic potential of mouse translation initiation factor 3 （TIF3） and elongation factor-1δ （TEF-1δ） in malignant transformed human bronchial epithelial cells induced by crystalline nickel sulfide （NiS）. Methods Abnormal expressions of human TIF3 and TEF-1δ genes in two kinds of NiS-transformed cells and NiS-tumorigenic cell lines were investigated and analyzed by the reverse transcript polymerase chain reaction （RT-PCR） and fluorescent quantitative polymerase chain reaction （FQ-PCR）, respectively. Results RT-PCR analysis primarily showed that both human TIF3 and TEF-1δ mRNA expressions in two kinds of NiS-transformed cells and NiS-tumorigenic cell lines were increased as compared with controls. FQ-PCR assay showed that the levels of TIF3 expressions in the transformed cells and tumorigenic cells were 3 and 4 times higher respectively, and the elevated expressions of TEF-16 eDNA copies were 2.7- to 3.5-fold in transformed cells and 4.1- to 5.2-fold in tumorigenic cells when compared with non-transformed cells, indicating that the over-expressions of human TIF3 and TEF-1δ genes were related to malignant degree of the cells induced by nickel. Conclusions These findings demonstrate that there are markedly abnormal expressions of TIF3 and TEF-1δ genes during malignant transformation of human bronchial epithelial cell lines induced by crystalline NiS. They seem to be the molecular mechanisms potentially responsible for human carcinogensis due to nickel.

Abrasive-assisted Nickel Electroforming Process with Moving Cathode

In traditional electroforming process for revolving parts with complex profiles, the drawbacks on surface of deposits, such as pinholes and nodules, will lead to varying physical and mechanical properties on different parts of electroformed components. To solve the problem, compositely moving cathode is employed in abrasive-assisted electroforming of revolving parts with complicated profiles. The cathode translates and rotates simultaneously to achieve uniform friction effect on deposits without drawbacks. The influences of current density and transla- tion speed on the microstructure and properties of the electroformed nickel layers are investigated. It is found that abrasive-assisted electroforming with compound cathode motion can effectively remove the pinholes and nodules, positively affect the crystal nucleation, and refine the grains of layer. The increase of current density will lead to coarse microstructure and lower micro hardness, from 325 HV down to 189 HV. While, faster translational linear speed produces better surface quality and higher micro hardness, from 236 HV up to 283 HV. The weld-ability of the electroformed layers are also studied through the metallurgical analysis of welded joints between nickel layer and 304 stainless steel. The electrodeposited nickel layer shows fine performance in welding. The novel compound motion of cathode promotes the mechanical properties and refines the microstructure of deposited layer.

Preparation of high emissivity NiCr_2O_4 powders with a spinel structure by spray drying

Spray-drying was used to produce the high emissivity NiCr2O4 powders with a spinel structure. Preliminary investigations focused on fabricating the high emissivity powders for infrared radiation coatings and finding the relationship between microstructure and emissivity. The NiCr2O4 powders were characterized for composition, microstructure, and infrared emissivity by X-ray diffraction （XRD）, scanning electron microscopy （SEM）, infrared radiant instrument, and Fourier transform infrared spectra （FT-IR）. Thermogravimetry and differential thermal analysis show that the appropriate baking temperature for NiCr2O4 powder preparation is about 1200？C. The emissivity measurement and FT-IR spectra show that, because of the special spinel structure, the NiCr2O4 powders have a high emissivity about 0.91. Spray-drying is a suitable method to produce the high emissivity ceramic powders.

Electrochemical properties of LiNi_(0.5)Mn_(1.3)Ti_(0.2)O_4/Li_4Ti_5O_(12)cells

Spinel compounds LiNi0.5Mn1.3Ti0.2O4（LNMTO） and Li4Ti5O12 （LTO） were synthesized by different methods. The particle sizes of LNMTO and LTO are 0.5-2 and 0.5-0.8 μm, respectively. The LNMTO/LTO cell exhibits better electrochemical properties at both a low current rate of 0.2C and a high current rate of 1C. When the specific capacity was determined based on the mass of the LNMTO cathode, the LNMTO/LTO cell delivered 137 mA.h.g-1 at 0.2C and 118.2 mA-h-g-l at 1C, and the corresponding capacity retentions after 30 cycles are 88.5% and 92.4%, respectively.

A facile preparation and electrochemical properties of nickel based compound-graphene sheet composites for supercapacitors

Composites of a nickel based compound incorporated with graphene sheets （NiBC-GS） are prepared by a simple flocculation, using hydrazine hydrate as flocculant and reductant, from a homogeneous intermixture of nickel dichloride and graphene oxide dispersed in N,N-dimethylformamide. Morphology, microstructure and thermal stability of the obtained products were characterized by field-emission scanning electron microscopy, X-ray diffraction and thermal gravimetric analysis. Furthermore, the electrochemical properties of NiBC-GS, as electrode materials for supercapacitors, were studied by cyclic voltammetry and galvanostatic charge]discharge in 2 mol L 1 KOH solution. It was determined that for NiBC-GS annealed at 250 ~C, a high specific capacitance of 2394 F g 1 was achieved at a current density of 1 A g^-1, with 78% of the value （i.e., 1864 F g^-1） retained after 5000 times of repeated galvanostatic charge/discharge cycling. The high specific capacitance and available charge/discharge stability indicate the synthesized NiBC-GS250 composite is a good candidate as a novel electrode material for supercapacitors.

Two Coordination Compounds Bearing Bis（2-dimethylaminoethyl） Ether： Syntheses, Crystal Structures and Catalytic Application to the Henry Reaction

Two novel coordination compounds, namely [Cu2（BDMAEE）（CH3COO）4],,（1） and [Ni（BDMAEE）Cl2]（2） [BDMAEE=bis（2-dimethylaminoethyl） ether], have been synthesized and characterized by IR, elemental analysis, PXRD and X-ray single crystal diffraction. In compound 1, the central Cu（Ⅱ） ion is coordinated with four oxygen atoms and one nitrogen atom, forming a distorted square pyramidal geometry. The asymmetric units composed of one Cu（Ⅱ） ion, two acetates and a half of BDMAEE are connected to form an infinite 1D chain structure by the bridging acetate and the BDMAEE. In compound 2, the central Ni（Ⅱ） ion is coordinated with one oxygen atom, two chlorine anions and two nitrogen atoms, forming a distorted square pyramidal geometry. The compounds exhibited excellent catalytic properties in the Henry reaction of nitromethane with some aromatic aldehydes, and the optimized reaction conditions were obtained.