Salt stress is one of the major abiotic stresses in agricultural plants worldwide.We used proteomics to analyze the differential expression of proteins in transgenic OsNAS1 and non-transformant Brassica napus treated ...Salt stress is one of the major abiotic stresses in agricultural plants worldwide.We used proteomics to analyze the differential expression of proteins in transgenic OsNAS1 and non-transformant Brassica napus treated with 20 mmol/L Na2CO3.Total protein from the leaves was extracted and separated through a high-resolution and highly repetitive two-dimensional electrophoresis(2-DE)technology system.Twelve protein spots were reproducibly observed to be upregulated by more than 2-fold between transgenic and non-transformant B.napus.These 12 spots were digested in-gel with trypsin and characterized by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS)to obtain the peptide mass fingerprints.Protein database searching revealed that 5 of these proteins are involved in salt tolerance:dehydrogenase,glutathione S-transferase,peroxidase,20S proteasome beta subunit,and ribulose-1,5-bisphosphate carboxylase/oxygenase.The potential functions of these identified proteins in substance and energy metabolism,stress tolerance,protein degradation,and cell defense are discussed.The salt tolerance of the transgenic rapeseed was significantly improved by the introduction of the OsNAS1 gene from Brazilian upland rice of Oryza sativa(cv.IAPAR 9).展开更多
基金supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),International S & T Cooperation of China(1021)the Chinese National Major Program of Transgenic Research(2009ZX08004-002B)the Scientific Research Foundation for the Introduction of Talent of Anhui Polytechnic University(2010YQQOO7)
文摘Salt stress is one of the major abiotic stresses in agricultural plants worldwide.We used proteomics to analyze the differential expression of proteins in transgenic OsNAS1 and non-transformant Brassica napus treated with 20 mmol/L Na2CO3.Total protein from the leaves was extracted and separated through a high-resolution and highly repetitive two-dimensional electrophoresis(2-DE)technology system.Twelve protein spots were reproducibly observed to be upregulated by more than 2-fold between transgenic and non-transformant B.napus.These 12 spots were digested in-gel with trypsin and characterized by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS)to obtain the peptide mass fingerprints.Protein database searching revealed that 5 of these proteins are involved in salt tolerance:dehydrogenase,glutathione S-transferase,peroxidase,20S proteasome beta subunit,and ribulose-1,5-bisphosphate carboxylase/oxygenase.The potential functions of these identified proteins in substance and energy metabolism,stress tolerance,protein degradation,and cell defense are discussed.The salt tolerance of the transgenic rapeseed was significantly improved by the introduction of the OsNAS1 gene from Brazilian upland rice of Oryza sativa(cv.IAPAR 9).
