Three new flavonol glycosides were isolated from the leaves of Helicia nilagirica Beed.. The structures were elucidated as kaempferol-3-O-b-D-xylopyranosyl-(16)-b-D-glyco- pyranosyl-(12)-a-L-rhamnopyranoside, querce...Three new flavonol glycosides were isolated from the leaves of Helicia nilagirica Beed.. The structures were elucidated as kaempferol-3-O-b-D-xylopyranosyl-(16)-b-D-glyco- pyranosyl-(12)-a-L-rhamnopyranoside, quercetin-3-O-b-D-xylopyranosyl-(16)-b-D-glycopy- ranosyl-(12)-[-b-D-xylopyranosyl-(14)]-a-L-rhamnopyranoside, and quercetin-3-O-b-D- xylopyranosyl-(16)-b-D-glycopyranosyl-(12)-a-L-rhamnopyranoside, named as Helicianeoside A、B and C, respectively.展开更多
Objective: To outline the antibacterial, antioxidant, a-glucosidase inhibition and anticancer properties of Michelia nilagirica(M. nilagirica) bark extract.Methods: The antibacterial activity of bark extracts against ...Objective: To outline the antibacterial, antioxidant, a-glucosidase inhibition and anticancer properties of Michelia nilagirica(M. nilagirica) bark extract.Methods: The antibacterial activity of bark extracts against human pathogens was assessed by disc diffusion assay. Phytochemical screening, total phenols, flavonoids content, antioxidant and a-glucosidase inhibition properties of bark extracts were investigated by standard methods. In vitro anticancer activity of ethyl acetate extract at various concentrations was observed against Hep G2 cells using MTT [3-(4, 5-dimethyl thiazol-2 yl)-2,5-diphenyl tetrazolium bromide] assay. The presence of diverse bioactive constituents in the ethyl acetate extract was identified using FT-IR and GC–MS analysis.Results: Ethyl acetate extract was found to be the promising agent against human pathogens tested. The ethyl acetate extracts showed the presence of various phytochemicals and comprised the substantial content of phenolics and flavonoids. The ethyl acetate extract showed better antioxidant activities and also revealed remarkable reducing power ability and a-glucosidase inhibition property. The dose dependent assay of extract showed remarkable cancer cell death with IC_(50) value of(303.26 ± 2.30) mg/m L. FTIR and GC–MS results indicated the presence of major bioactive constituents in the ethyl acetate extract of M. nilagirica bark.Conclusions: Revealing the first report on in vitro biological properties and chemical composition analysis of M. nilagirica bark extract, our study implied that this plant could be of great importance in food and pharmaceutical industries.展开更多
Artemisinin, an endoperoxide sesquiterpene lactone has proven effective in treating drug resistant cases of malaria and cancer. Artemisia annua [sweet wormwood] is the sole source for artemisinin production in many co...Artemisinin, an endoperoxide sesquiterpene lactone has proven effective in treating drug resistant cases of malaria and cancer. Artemisia annua [sweet wormwood] is the sole source for artemisinin production in many countries. To counter the low content in leaves and costly chemical synthesis process in India, alternative ways to produce artemisinin have been sought. In current study, we collected A. pallens, A. japonica and A. nilagirica from Western Ghats of Maharashtra, India and analyzed artemisinin content. Samples were extracted from leaves and florets in various extraction conditions and analyzed using different chromatographic techniques. Thin layer chromatography (TLC) and high performance thin layer chromatography (HPTLC) analysis showed the presence of compound with endoperoxide linkage in A. nilagirica. High performance liquid chromatography (HPLC) analysis showed the detection of artemisinin in methylene dichloride florets extract of A. japonica, but the concentration was too low [1.3 mg/g dry wt.] for further analyses. Gas chromatography/mass spectrometry (GC/MS) analysis identified structurally important components in the A. nilagirica ethyl acetate extract which explores the biosynthetic pathway of artemisinin from its most important precursor amorpha-4,11-diene. This is the first report of chromatographic screening of these Indian varieties of Artemisia spp. for artemisinin content.展开更多
文摘Three new flavonol glycosides were isolated from the leaves of Helicia nilagirica Beed.. The structures were elucidated as kaempferol-3-O-b-D-xylopyranosyl-(16)-b-D-glyco- pyranosyl-(12)-a-L-rhamnopyranoside, quercetin-3-O-b-D-xylopyranosyl-(16)-b-D-glycopy- ranosyl-(12)-[-b-D-xylopyranosyl-(14)]-a-L-rhamnopyranoside, and quercetin-3-O-b-D- xylopyranosyl-(16)-b-D-glycopyranosyl-(12)-a-L-rhamnopyranoside, named as Helicianeoside A、B and C, respectively.
基金supported by Rajiv Gandhi National Fellowship (RGNF-2013-14-ST-TAM-48307),University Grants Commission,Delhi,India
文摘Objective: To outline the antibacterial, antioxidant, a-glucosidase inhibition and anticancer properties of Michelia nilagirica(M. nilagirica) bark extract.Methods: The antibacterial activity of bark extracts against human pathogens was assessed by disc diffusion assay. Phytochemical screening, total phenols, flavonoids content, antioxidant and a-glucosidase inhibition properties of bark extracts were investigated by standard methods. In vitro anticancer activity of ethyl acetate extract at various concentrations was observed against Hep G2 cells using MTT [3-(4, 5-dimethyl thiazol-2 yl)-2,5-diphenyl tetrazolium bromide] assay. The presence of diverse bioactive constituents in the ethyl acetate extract was identified using FT-IR and GC–MS analysis.Results: Ethyl acetate extract was found to be the promising agent against human pathogens tested. The ethyl acetate extracts showed the presence of various phytochemicals and comprised the substantial content of phenolics and flavonoids. The ethyl acetate extract showed better antioxidant activities and also revealed remarkable reducing power ability and a-glucosidase inhibition property. The dose dependent assay of extract showed remarkable cancer cell death with IC_(50) value of(303.26 ± 2.30) mg/m L. FTIR and GC–MS results indicated the presence of major bioactive constituents in the ethyl acetate extract of M. nilagirica bark.Conclusions: Revealing the first report on in vitro biological properties and chemical composition analysis of M. nilagirica bark extract, our study implied that this plant could be of great importance in food and pharmaceutical industries.
文摘Artemisinin, an endoperoxide sesquiterpene lactone has proven effective in treating drug resistant cases of malaria and cancer. Artemisia annua [sweet wormwood] is the sole source for artemisinin production in many countries. To counter the low content in leaves and costly chemical synthesis process in India, alternative ways to produce artemisinin have been sought. In current study, we collected A. pallens, A. japonica and A. nilagirica from Western Ghats of Maharashtra, India and analyzed artemisinin content. Samples were extracted from leaves and florets in various extraction conditions and analyzed using different chromatographic techniques. Thin layer chromatography (TLC) and high performance thin layer chromatography (HPTLC) analysis showed the presence of compound with endoperoxide linkage in A. nilagirica. High performance liquid chromatography (HPLC) analysis showed the detection of artemisinin in methylene dichloride florets extract of A. japonica, but the concentration was too low [1.3 mg/g dry wt.] for further analyses. Gas chromatography/mass spectrometry (GC/MS) analysis identified structurally important components in the A. nilagirica ethyl acetate extract which explores the biosynthetic pathway of artemisinin from its most important precursor amorpha-4,11-diene. This is the first report of chromatographic screening of these Indian varieties of Artemisia spp. for artemisinin content.
