O6 -methylguanine- DNA- methyltransferase (MGMT ) plays a very important role in the cellular resis- tance to nitrosoureas drugs. Inhibition of MGMT might be a useful approach in tumor chemotherapy. In this study, the...O6 -methylguanine- DNA- methyltransferase (MGMT ) plays a very important role in the cellular resis- tance to nitrosoureas drugs. Inhibition of MGMT might be a useful approach in tumor chemotherapy. In this study, the depletion of MGMT activity by retroviral-mediated antisense RNA transfection were reported. Three retroviral vectors expressing MGMT antisense RNA were constructed and transfected into HeLa S3 cells. The difference of MGMT mRNA, MGMT activity as well as cellular resistance to ACNU before and after transfection were observed. It was found that antisense RNA targeting 5’region and whole length of MGMT mRNA could partially deplete MGMT activity and enhance killing effects of ACNU. However, 3’ region antisense RNA had no effect on MGMT modulation.展开更多
IM To compare the efficacy between a combined chemotherapy of ACNU (nimustine hydrochloride, brand name Nidran), a new nitrosourea agent, and a combined chemotherapy of methylCCNU for advanced gastric cancer.METHOD ...IM To compare the efficacy between a combined chemotherapy of ACNU (nimustine hydrochloride, brand name Nidran), a new nitrosourea agent, and a combined chemotherapy of methylCCNU for advanced gastric cancer.METHOD One hundred and three cases of advanced gastric cancer were randomly allocated into Group A (MeCCNU, 5FU and ADM) and Group B (ACNU, 5FU and ADM). The quality of life (QOL) questionnaire composed of 11 ordinal categorical items was studied in these patients.RESULTS In Group A there was no complete remission (CR) or partial remission (PR), while in Group B, although there was no CR, there were 8 PR (8/46 cases), denoting the response rate of 0% in Group A and 174% in Group B. The median survival time in Group A was 108 days and in Group B 112 days. Both groups tolerated well during the treatment and there were no serious adverse effects. QOL evaluations showed better psychological and physical feelings of tiredness in Group B than in Group A, and scores of face scale had more pleasant inclination.CONCLUSION ACNU combination is superior to MeCCNU combination for advanced gastric cancer.展开更多
Twenty four cases of advanced gastric cancer were divided into two groups according to the general condition of the patients (PS 0-2 and 3-4), further subdivided into localized abdominal form, liver metastatic form, a...Twenty four cases of advanced gastric cancer were divided into two groups according to the general condition of the patients (PS 0-2 and 3-4), further subdivided into localized abdominal form, liver metastatic form, ascitic form and distant metastatic form. These patients were allocated randomly into Regimen A or B. Regimen A: Tegafur 600 mg daily and Mitomycin C (MMC) 5 mg/m2/1-2 weeks. Regimen B: Tegafur 600 mg daily and ACNU (Nimustine, water-soluble Nitrosourea) 60 mg/week for 2 weeks. After a course of ACNU, it was with drawn for 4 weeks and then resumed again. The response rate of Regimen A was 3/11 (27%), that of Regimen B was 3/13 (23%).展开更多
基金This project is supported by National Foundation of Natural sciences of China.
文摘O6 -methylguanine- DNA- methyltransferase (MGMT ) plays a very important role in the cellular resis- tance to nitrosoureas drugs. Inhibition of MGMT might be a useful approach in tumor chemotherapy. In this study, the depletion of MGMT activity by retroviral-mediated antisense RNA transfection were reported. Three retroviral vectors expressing MGMT antisense RNA were constructed and transfected into HeLa S3 cells. The difference of MGMT mRNA, MGMT activity as well as cellular resistance to ACNU before and after transfection were observed. It was found that antisense RNA targeting 5’region and whole length of MGMT mRNA could partially deplete MGMT activity and enhance killing effects of ACNU. However, 3’ region antisense RNA had no effect on MGMT modulation.
文摘IM To compare the efficacy between a combined chemotherapy of ACNU (nimustine hydrochloride, brand name Nidran), a new nitrosourea agent, and a combined chemotherapy of methylCCNU for advanced gastric cancer.METHOD One hundred and three cases of advanced gastric cancer were randomly allocated into Group A (MeCCNU, 5FU and ADM) and Group B (ACNU, 5FU and ADM). The quality of life (QOL) questionnaire composed of 11 ordinal categorical items was studied in these patients.RESULTS In Group A there was no complete remission (CR) or partial remission (PR), while in Group B, although there was no CR, there were 8 PR (8/46 cases), denoting the response rate of 0% in Group A and 174% in Group B. The median survival time in Group A was 108 days and in Group B 112 days. Both groups tolerated well during the treatment and there were no serious adverse effects. QOL evaluations showed better psychological and physical feelings of tiredness in Group B than in Group A, and scores of face scale had more pleasant inclination.CONCLUSION ACNU combination is superior to MeCCNU combination for advanced gastric cancer.
文摘Twenty four cases of advanced gastric cancer were divided into two groups according to the general condition of the patients (PS 0-2 and 3-4), further subdivided into localized abdominal form, liver metastatic form, ascitic form and distant metastatic form. These patients were allocated randomly into Regimen A or B. Regimen A: Tegafur 600 mg daily and Mitomycin C (MMC) 5 mg/m2/1-2 weeks. Regimen B: Tegafur 600 mg daily and ACNU (Nimustine, water-soluble Nitrosourea) 60 mg/week for 2 weeks. After a course of ACNU, it was with drawn for 4 weeks and then resumed again. The response rate of Regimen A was 3/11 (27%), that of Regimen B was 3/13 (23%).
