Iwagaki oyster Crassostrea nippona occurs naturally along the coasts of Japan and Korea. Because of its unique flavor, delicious taste, edibility during the summer and high commercial value, it has been identified as ...Iwagaki oyster Crassostrea nippona occurs naturally along the coasts of Japan and Korea. Because of its unique flavor, delicious taste, edibility during the summer and high commercial value, it has been identified as a potential aquaculture species. To determine the optimum aquaculture conditions and provide necessary information for mass production of the juvenile, the effects of six salinities(15, 20, 25, 30, 35 and 40) and five temperatures(16, 20, 24, 28 and 32℃) on growth and survival of juvenile C. nippona were examined in this study. In the salinity experiment, the largest values of mean shell height and growth rate were observed at salinity 25(20.96 ± 0.36 mm and 172.0 μm d^(-1), respectively), which were significantly different(P < 0.05) with those of other treatments, except at salinity 30(20.56 ± 1.05 mm and 160.3 μm d^(-1), respectively)(P > 0.05). The maximum survival rate 84.44% was always observed at salinity 20, and there was no significant difference(P > 0.05) in survival rate among salinities varying between 15 and 35. In the temperature-related experiments, the highest growth and survival rates of juvenile were observed at 24℃(180.8 μm d^(-1) and 84.4%) and 28℃(190.7 μm d^(-1) and 83.3%), respectively, on day 20, and showed significantly(P < 0.05) larger size and higher survival rate than any other groups. Both juvenile survival and growth were significantly depressed at extreme salinities(15, 40) and temperatures(16℃, 32℃). Based on the results of the present study, a salinity range from 25 to 30 and a temperature range from 24℃ to 28℃ are considered optimal conditions for survival and growth of juvenile C. nippona.展开更多
Iwagaki oyster,Crassostrea nippona,widely distributes along the seashore of Eastern Asia,and has been considered to be a potential breeding species due to its delicious taste and high commercial value.In order to stud...Iwagaki oyster,Crassostrea nippona,widely distributes along the seashore of Eastern Asia,and has been considered to be a potential breeding species due to its delicious taste and high commercial value.In order to study its genetic background and population structure,we developed 46 novel polymorphic microsatellite markers using next-generation sequencing technique and characterized them in 30 individuals.The number of alleles ranged from 3 to 22,while the observed and expected heterozygosities varied from 0.133 to 1.000 and 0.455 to 0.949,respectively.Fifteen microsatellite markers were selected and grouped into five highly informative multiplex PCRs for C.nippona.We evaluated and validated these multiplex PCRs in a cultured population including 173 candidate parents and 486 offspring.In actual parentage analysis,80%of the offspring were correctly assigned to their parental pairs using three multiplex PCRs.Furthermore,the success rate of parentage assignment reached 96%when the other two multiplex PCRs were added.These 46 microsatellite loci with high variability and the five multiplex PCRs described here provide a powerful tool for pedigree reconstruction,resource conservation and selective breeding program of C.nippona.展开更多
Hypo-salinity can reduce the immunological reaction in Crassostrea nippona,even lead to massive mortality.It is important to understand the molecular mechanism of oyster defense system,while quantitative real-time PCR...Hypo-salinity can reduce the immunological reaction in Crassostrea nippona,even lead to massive mortality.It is important to understand the molecular mechanism of oyster defense system,while quantitative real-time PCR can be employed in the study.However,the accuracy of quantitative real-time PCR relies on the use of suitable reference genes.In this study,the expression stability of 14 candidate reference genes including traditional housekeeping genes EF1A,TUB,TUA,GAPDH,RO21,as well as new candidate reference genes RPL5,RPL8,RPS27,RPL14,RPL4,CO3,RPS8,RPS4,CYTB in different tissues of C.nippona under salinity stress has been validated by quantitative real-time PCR.Ribosomal protein genes selected through expression analysis of transcriptome data from C.nippona generally were more stable than traditional reference genes.According to the geNorm analysis,RPL4 and RPS4 could be used as internal controls for studying gene expression in C.nippona with real-time PCR under salinity stress.展开更多
基金supported by the grants from the Key Research and Development Program of Shandong Province(No.2016ZDJS06A06)the National Natural Science Foundation of China(No.31772843)the Major Project for Tianjin Seed Technology(No.15ZXZYNC00 050)
文摘Iwagaki oyster Crassostrea nippona occurs naturally along the coasts of Japan and Korea. Because of its unique flavor, delicious taste, edibility during the summer and high commercial value, it has been identified as a potential aquaculture species. To determine the optimum aquaculture conditions and provide necessary information for mass production of the juvenile, the effects of six salinities(15, 20, 25, 30, 35 and 40) and five temperatures(16, 20, 24, 28 and 32℃) on growth and survival of juvenile C. nippona were examined in this study. In the salinity experiment, the largest values of mean shell height and growth rate were observed at salinity 25(20.96 ± 0.36 mm and 172.0 μm d^(-1), respectively), which were significantly different(P < 0.05) with those of other treatments, except at salinity 30(20.56 ± 1.05 mm and 160.3 μm d^(-1), respectively)(P > 0.05). The maximum survival rate 84.44% was always observed at salinity 20, and there was no significant difference(P > 0.05) in survival rate among salinities varying between 15 and 35. In the temperature-related experiments, the highest growth and survival rates of juvenile were observed at 24℃(180.8 μm d^(-1) and 84.4%) and 28℃(190.7 μm d^(-1) and 83.3%), respectively, on day 20, and showed significantly(P < 0.05) larger size and higher survival rate than any other groups. Both juvenile survival and growth were significantly depressed at extreme salinities(15, 40) and temperatures(16℃, 32℃). Based on the results of the present study, a salinity range from 25 to 30 and a temperature range from 24℃ to 28℃ are considered optimal conditions for survival and growth of juvenile C. nippona.
基金supported by the National Natural Science Foundation of China (No. 31772843)the Natural Sci- ence Foundation of Guangxi Province (No. AA17204080-4)the Fundamental Research Funds for the Central Uni- versities (No. 201762014)
文摘Iwagaki oyster,Crassostrea nippona,widely distributes along the seashore of Eastern Asia,and has been considered to be a potential breeding species due to its delicious taste and high commercial value.In order to study its genetic background and population structure,we developed 46 novel polymorphic microsatellite markers using next-generation sequencing technique and characterized them in 30 individuals.The number of alleles ranged from 3 to 22,while the observed and expected heterozygosities varied from 0.133 to 1.000 and 0.455 to 0.949,respectively.Fifteen microsatellite markers were selected and grouped into five highly informative multiplex PCRs for C.nippona.We evaluated and validated these multiplex PCRs in a cultured population including 173 candidate parents and 486 offspring.In actual parentage analysis,80%of the offspring were correctly assigned to their parental pairs using three multiplex PCRs.Furthermore,the success rate of parentage assignment reached 96%when the other two multiplex PCRs were added.These 46 microsatellite loci with high variability and the five multiplex PCRs described here provide a powerful tool for pedigree reconstruction,resource conservation and selective breeding program of C.nippona.
基金This study was supported by grants from the National Natural Science Foundation of China(No.31772843)the Natural Science Foundation of Guangxi Province(No.AA17204080-4)+1 种基金the Fundamental Research Funds for the Central Universities(No.201762014)and the Ocean University of China-Auburn University Joint Research Center for Aquaculture and Environmental Science.
文摘Hypo-salinity can reduce the immunological reaction in Crassostrea nippona,even lead to massive mortality.It is important to understand the molecular mechanism of oyster defense system,while quantitative real-time PCR can be employed in the study.However,the accuracy of quantitative real-time PCR relies on the use of suitable reference genes.In this study,the expression stability of 14 candidate reference genes including traditional housekeeping genes EF1A,TUB,TUA,GAPDH,RO21,as well as new candidate reference genes RPL5,RPL8,RPS27,RPL14,RPL4,CO3,RPS8,RPS4,CYTB in different tissues of C.nippona under salinity stress has been validated by quantitative real-time PCR.Ribosomal protein genes selected through expression analysis of transcriptome data from C.nippona generally were more stable than traditional reference genes.According to the geNorm analysis,RPL4 and RPS4 could be used as internal controls for studying gene expression in C.nippona with real-time PCR under salinity stress.
