Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing b...Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing blood pressure. However, its effects on gastric ulcer have not been investigated. This study aimed to investigate the effects of the Shibi tea water extract(STE) and its main flavonoid camellianin A(CA) in hydrochloric acid(HCl) and ethanol(Et OH)-induced acute gastric ulcer in mice. Administration of CA and STE for continuous two days after stimulation by HCl/Et OH significantly attenuated the deterioration of gastric mucosal damage by lowering the gross gastric mucosal index, histopathological injury index, the oxidative stress, the expression of the inflammatory cytokines TNF-α and IL-6, and the expression of inflammatory mediators i NOS and COX-2. Western blotting and immunohistochemistry analysis showed that CA and STE regulated the inflammatory signaling pathway protein levels of IκB-α and NF-κB. Taken together, our study verified that CA and STE have antioxidative and anti-inflammatory effects in gastric ulcer mice. We propose that A. nitida should be developed as natural functional food for acute gastric ulcer patients base on the gastroprotective effects of STE and its main flavonoid CA.展开更多
[Objective] The research aimed to study the transferability of Camellia sinensis EST-SSRs in Theaceae plant.[Method] Seven pairs of EST-SSRs primers which derived from Camellia sinensis EST sequence were used to ampli...[Objective] The research aimed to study the transferability of Camellia sinensis EST-SSRs in Theaceae plant.[Method] Seven pairs of EST-SSRs primers which derived from Camellia sinensis EST sequence were used to amplify the nineteen materials of Theaceae plant.[Result] Five pairs in the seven pairs of EST-SSRs primers could effectively amplify the nineteen tested varieties,and the transferability rate was 71.43%.The amplification rate of Camellia retiacalate FengShanCha,Camellia japouica CaiXia and Camellia retiacalate JuBan was the highest.The amplification rate of Camellia synaptica Sealy and Adinandra sagonica var.wallichiana（oc）Ming was the lowest.Moreover,four pairs in the five pairs of primers which could effectively amplify showed the rich polymorphism whose difference was obvious in the tested materials.[Conclusion] SSR primers which were developed from Camellia sinensis genome had the higher transferability in the different genus and species of Theaceae plant,could be used in the comparative genome research and analysis mark research of Theaceae plant.展开更多
Millettia nitida var. hirsutissima is a Chinese herbal medicine used for the treatment of gynecological diseases. An HPLC/DAD/ESI-MSn method was established for the rapid separation and characterization of bioactive f...Millettia nitida var. hirsutissima is a Chinese herbal medicine used for the treatment of gynecological diseases. An HPLC/DAD/ESI-MSn method was established for the rapid separation and characterization of bioactive flavonoids in M. nitida var. hirsutissima. A total of 32 flavonoids were detected, of which 14 compounds were unambiguously characterized by comparing their retention time, UV, and MS spectra with those of the reference standards, and the others were tentatively identified based on their tandem mass spectrometry fragmentation data obtained in the negative ionization mode on line. Nineteen of these compounds characterized were reported from this plant for the first time.展开更多
Picralima nitida Durand and Hook,(fam.Apocynaceae)is a West African plant with varied applications in African folk medicine.Various parts of the plant have been employed ethnomedicinally as remedy for fever,hypertensi...Picralima nitida Durand and Hook,(fam.Apocynaceae)is a West African plant with varied applications in African folk medicine.Various parts of the plant have been employed ethnomedicinally as remedy for fever,hypertension,jaundice,dysmenorrheal,gastrointestinal disorders and malaria.In order to reveal its full pharmacological and therapeutic potentials,die present review focuses on the current medicinal uses,phytochemistry,pharmacological and toxicological activities of this species.Literature survey on scientific journals,books as well as electronic sources have shown the isolation of alkaloids,tannins,polyphenols and steroids from different parts of the plant,pharmacological studies revealed that the extract or isolated compounds from this species posses analgesic,anti-inflammatory,hypoglyceamic,hypotensive,antiplasmodial,antimicrobial,antiulcer and antitumorigenic activities.Results from various scientific investigations to date have revealed the potential of the extract from the plant or isolated compounds for use in the treatment and prevention of various kinds of human diseases.However,further studies on the extracts and pure compounds from this species is required to completely assess its phytoc-hemical,pharmacological and toxicological profile as well as the mechanism of action behind these pharmacological activities exhibited by the compounds isolated from this species.展开更多
Three new indole glycosides 22-deoxystrictosamide(1),22-deoxystrictosamide Nb-oxide(2)and vincosamide 20-O-b-D-xylopyranoside-11-O-b-D-glucopyranoside(3),together with four known analogues were isolated from aqueous f...Three new indole glycosides 22-deoxystrictosamide(1),22-deoxystrictosamide Nb-oxide(2)and vincosamide 20-O-b-D-xylopyranoside-11-O-b-D-glucopyranoside(3),together with four known analogues were isolated from aqueous fraction of Strychnos nitida.Their structures were elucidated on the basis of extensive analysis of spectroscopic data.All the alkaloids were tested for their cytotoxic activity,but they did not show any exciting result.展开更多
Objective:To investigated the antisecrectory activities of the methanol extract,chloroform fraction and methanol fraction of Picralima nitida seeds.Methods:The methanol extract of Picralima nitida seeds was fractionat...Objective:To investigated the antisecrectory activities of the methanol extract,chloroform fraction and methanol fraction of Picralima nitida seeds.Methods:The methanol extract of Picralima nitida seeds was fractionated into chloroform fraction and methanol fraction.They were evaluated for antiulcer activity and gastric emptying time in rats using aspirin-pylorus-ligation model.Results:Oral administration of the methanol extract,chloroform fraction and methanol fraction at 1 000 mg/kg reduced gastric ulcer by 56.4%,40.0%and 56.3%,respectively;and the fractions of the extract significantly(P【0.05) reduced gastric emptying time when compared to the control.Gastric acidity was significandy decreased when compared with saline group,40.25 mEq/ L in methanol extract,50.0 mEq/L in chloroform fraction 51.25 mEq/L in methanol fration but had no significant effect on the gastric secretion volume.Conclusions:These findings showed that methanol extract,chloroform fraction and methanol fraction of the seeds of Picralima possessed potent antiulcer properties and some antisecretory properties.展开更多
The conservation of cola nuts (Cola nitida) poses a real problem in Côte d’Ivoire because of the post-harvest losses. The objective of this study was to evaluate the impact of post-harvest technologies on o...The conservation of cola nuts (Cola nitida) poses a real problem in Côte d’Ivoire because of the post-harvest losses. The objective of this study was to evaluate the impact of post-harvest technologies on organoleptic and physical properties of cola nut during storage. A biopesticide, glucose syrup and biopesticide + glucose syrup were applied to fresh cola nuts before conditioning and kept at 28°C for 6 weeks. Physicochemical and sensory analyses were performed to check the quality of the nuts during storage. The results showed that the biopesticide keep cola nuts better than the others methods with only 11.66% ± 3.04% and 13.66% ± 3.95% of loss rates for white and red cola nuts respectively. Cola nuts treated with bio-pesticide retain significantly their freshness with 62.00% ± 1.15% of moisture for white nuts and 64.00% ± 2.00% of moisture for red nuts compared to those treated with glucose syrup and bio-pesticide + glucose syrup (56.66% ± 1.15%). Cola nuts treated with biopesticide have a better acceptability compared to those subjected to others treatments. The use of biopesticide for the storage of cola nuts minimizes the losses and maintains the quality whatever the type of cola.展开更多
The antioxidant activity and the total phenolic content of Okoubaka aubrevillei(Anunuebe)and Pychnobotrya nitida(Osu)plant extracts were investigated in this study to justify their ethno medicinal importance.The antio...The antioxidant activity and the total phenolic content of Okoubaka aubrevillei(Anunuebe)and Pychnobotrya nitida(Osu)plant extracts were investigated in this study to justify their ethno medicinal importance.The antioxidant activity of crude extracts of both plants was investigated using the scavenging effect on DPPH(2,2-Diphenyl-1-Picrylhydrazyl Radical)assay,while the total phenolic content was determined by the Folin-Ciocalteu reagent method.When compared to standard drug vitamin C at various concentrations,both extracts exhibited potent antioxidant activity that increased with increasing concentration of extracts.The high positive linear correlation between the antioxidant capacity and the total phenolic content suggests that the antioxidant capacity of both plants can be largely attributed to their phenolic compounds.Based on these findings,O.aubrevillei fruit and P.nitida leaf could be potential natural antioxidant sources for preventing free radical-induced diseases.展开更多
基金funded by the “14th Five-Year Plan” team-building projects of Guangdong Academy Agricultural Sciences (202126TD)National Natural Science Foundation of China (81903319, 81803236, 31800295)+10 种基金Guangdong Science and Technology program (2018KJYZ002)Guangdong Basic and Applied Basic Research Foundation (2020A1515011266)Qingyuan Science and Technology Program (DZXQY021, 181022114566189, 181022114566189, 2019A039, 2020KJJH042)Shaoguan Science and Technology Program (2018CS11902, 2018sn081)Guangzhou Science and Technology Program (202002030202)Zhaoqing Science and Technology Program (2019N001, 2019N013)Maoming Science and Technology Program (mmkj2020045)Zhanjiang Science and Technology Program (2020A03014)Yingde Science and Technology Board (JHXM2018029)Innovation Fund projects of Guangdong Academy of Agricultural Sciences (202115)Special fund for scientific innovation strategy-construction of high level Academy of Agriculture Science (R2019PY-JX004, R2018YJ-YB3002, R2016YJYB3003, R2018PY-QF005, R2018QD-101)。
文摘Gastric ulcer is a global health concern nowadays. Adinandra nitida, known as Shibi tea, is a flavonoidrich plant found in South China. A. nitida possesses many healthy properties, such as antioxidation and reducing blood pressure. However, its effects on gastric ulcer have not been investigated. This study aimed to investigate the effects of the Shibi tea water extract(STE) and its main flavonoid camellianin A(CA) in hydrochloric acid(HCl) and ethanol(Et OH)-induced acute gastric ulcer in mice. Administration of CA and STE for continuous two days after stimulation by HCl/Et OH significantly attenuated the deterioration of gastric mucosal damage by lowering the gross gastric mucosal index, histopathological injury index, the oxidative stress, the expression of the inflammatory cytokines TNF-α and IL-6, and the expression of inflammatory mediators i NOS and COX-2. Western blotting and immunohistochemistry analysis showed that CA and STE regulated the inflammatory signaling pathway protein levels of IκB-α and NF-κB. Taken together, our study verified that CA and STE have antioxidative and anti-inflammatory effects in gastric ulcer mice. We propose that A. nitida should be developed as natural functional food for acute gastric ulcer patients base on the gastroprotective effects of STE and its main flavonoid CA.
基金Supported by"Provincial and Ministerial Key Discipline,Provincial Key Laboratory of University and School Laboratory Sharing Platform" ItemSouthwest Forestry University Key Fund Item(110909)~~
文摘[Objective] The research aimed to study the transferability of Camellia sinensis EST-SSRs in Theaceae plant.[Method] Seven pairs of EST-SSRs primers which derived from Camellia sinensis EST sequence were used to amplify the nineteen materials of Theaceae plant.[Result] Five pairs in the seven pairs of EST-SSRs primers could effectively amplify the nineteen tested varieties,and the transferability rate was 71.43%.The amplification rate of Camellia retiacalate FengShanCha,Camellia japouica CaiXia and Camellia retiacalate JuBan was the highest.The amplification rate of Camellia synaptica Sealy and Adinandra sagonica var.wallichiana（oc）Ming was the lowest.Moreover,four pairs in the five pairs of primers which could effectively amplify showed the rich polymorphism whose difference was obvious in the tested materials.[Conclusion] SSR primers which were developed from Camellia sinensis genome had the higher transferability in the different genus and species of Theaceae plant,could be used in the comparative genome research and analysis mark research of Theaceae plant.
基金supported by the 985 Project of Peking University Health Science Center (No. 985-2-119-121)
文摘Millettia nitida var. hirsutissima is a Chinese herbal medicine used for the treatment of gynecological diseases. An HPLC/DAD/ESI-MSn method was established for the rapid separation and characterization of bioactive flavonoids in M. nitida var. hirsutissima. A total of 32 flavonoids were detected, of which 14 compounds were unambiguously characterized by comparing their retention time, UV, and MS spectra with those of the reference standards, and the others were tentatively identified based on their tandem mass spectrometry fragmentation data obtained in the negative ionization mode on line. Nineteen of these compounds characterized were reported from this plant for the first time.
基金supported by a US-Senior Fulbright Award granted to Dr.A.Falodun for study at University of Mississippi,USA,CIESCs for the Fulbright awardthe University of Benin(Nigeria)and Institute of Chemistry(University of Rostock,Germany),for their support
文摘Picralima nitida Durand and Hook,(fam.Apocynaceae)is a West African plant with varied applications in African folk medicine.Various parts of the plant have been employed ethnomedicinally as remedy for fever,hypertension,jaundice,dysmenorrheal,gastrointestinal disorders and malaria.In order to reveal its full pharmacological and therapeutic potentials,die present review focuses on the current medicinal uses,phytochemistry,pharmacological and toxicological activities of this species.Literature survey on scientific journals,books as well as electronic sources have shown the isolation of alkaloids,tannins,polyphenols and steroids from different parts of the plant,pharmacological studies revealed that the extract or isolated compounds from this species posses analgesic,anti-inflammatory,hypoglyceamic,hypotensive,antiplasmodial,antimicrobial,antiulcer and antitumorigenic activities.Results from various scientific investigations to date have revealed the potential of the extract from the plant or isolated compounds for use in the treatment and prevention of various kinds of human diseases.However,further studies on the extracts and pure compounds from this species is required to completely assess its phytoc-hemical,pharmacological and toxicological profile as well as the mechanism of action behind these pharmacological activities exhibited by the compounds isolated from this species.
基金the National Natural Science Foundation of China(81225024).
文摘Three new indole glycosides 22-deoxystrictosamide(1),22-deoxystrictosamide Nb-oxide(2)and vincosamide 20-O-b-D-xylopyranoside-11-O-b-D-glucopyranoside(3),together with four known analogues were isolated from aqueous fraction of Strychnos nitida.Their structures were elucidated on the basis of extensive analysis of spectroscopic data.All the alkaloids were tested for their cytotoxic activity,but they did not show any exciting result.
文摘Objective:To investigated the antisecrectory activities of the methanol extract,chloroform fraction and methanol fraction of Picralima nitida seeds.Methods:The methanol extract of Picralima nitida seeds was fractionated into chloroform fraction and methanol fraction.They were evaluated for antiulcer activity and gastric emptying time in rats using aspirin-pylorus-ligation model.Results:Oral administration of the methanol extract,chloroform fraction and methanol fraction at 1 000 mg/kg reduced gastric ulcer by 56.4%,40.0%and 56.3%,respectively;and the fractions of the extract significantly(P【0.05) reduced gastric emptying time when compared to the control.Gastric acidity was significandy decreased when compared with saline group,40.25 mEq/ L in methanol extract,50.0 mEq/L in chloroform fraction 51.25 mEq/L in methanol fration but had no significant effect on the gastric secretion volume.Conclusions:These findings showed that methanol extract,chloroform fraction and methanol fraction of the seeds of Picralima possessed potent antiulcer properties and some antisecretory properties.
文摘The conservation of cola nuts (Cola nitida) poses a real problem in Côte d’Ivoire because of the post-harvest losses. The objective of this study was to evaluate the impact of post-harvest technologies on organoleptic and physical properties of cola nut during storage. A biopesticide, glucose syrup and biopesticide + glucose syrup were applied to fresh cola nuts before conditioning and kept at 28°C for 6 weeks. Physicochemical and sensory analyses were performed to check the quality of the nuts during storage. The results showed that the biopesticide keep cola nuts better than the others methods with only 11.66% ± 3.04% and 13.66% ± 3.95% of loss rates for white and red cola nuts respectively. Cola nuts treated with bio-pesticide retain significantly their freshness with 62.00% ± 1.15% of moisture for white nuts and 64.00% ± 2.00% of moisture for red nuts compared to those treated with glucose syrup and bio-pesticide + glucose syrup (56.66% ± 1.15%). Cola nuts treated with biopesticide have a better acceptability compared to those subjected to others treatments. The use of biopesticide for the storage of cola nuts minimizes the losses and maintains the quality whatever the type of cola.
文摘The antioxidant activity and the total phenolic content of Okoubaka aubrevillei(Anunuebe)and Pychnobotrya nitida(Osu)plant extracts were investigated in this study to justify their ethno medicinal importance.The antioxidant activity of crude extracts of both plants was investigated using the scavenging effect on DPPH(2,2-Diphenyl-1-Picrylhydrazyl Radical)assay,while the total phenolic content was determined by the Folin-Ciocalteu reagent method.When compared to standard drug vitamin C at various concentrations,both extracts exhibited potent antioxidant activity that increased with increasing concentration of extracts.The high positive linear correlation between the antioxidant capacity and the total phenolic content suggests that the antioxidant capacity of both plants can be largely attributed to their phenolic compounds.Based on these findings,O.aubrevillei fruit and P.nitida leaf could be potential natural antioxidant sources for preventing free radical-induced diseases.
