Osteoporosis results in decreased bone mass and insufficient osteogenic function.Existing titanium alloy implants have insufficient osteoinductivity and delayed/incomplete fracture union can occur when used to treat o...Osteoporosis results in decreased bone mass and insufficient osteogenic function.Existing titanium alloy implants have insufficient osteoinductivity and delayed/incomplete fracture union can occur when used to treat osteoporotic fractures.Copper ions have good osteogenic activity,but their dose-dependent cytotoxicity limits their clinical use for bone implants.In this study,titanium alloy implants functionalized with a TiCu/TiCuN coating by arc ion plating achieved a controlled release of copper ions in vitro for 28 days.The coated alloy was co-cultured with bone marrow mesenchymal stem cells and showed excellent biocompatibility and osteoinductivity in vitro.A further exploration of the underlying mechanism by quantitative real-time polymerase chain reaction and western blotting revealed that the enhancement effects are related to the upregulation of genes and proteins(such as axin2,β-catenin,GSK-3β,p-GSK-3β,LEF1 and TCF1/TCF7)involved in the Wnt/β-catenin pathway.In vivo experiments showed that the TiCu/TiCuN coating significantly promoted osteoporotic fracture healing in a rat femur fracture model,and has good in vivo biocompatibility based on various staining results.Our study confirmed that TiCu/TiCuN-coated Ti promotes osteoporotic fracture healing associated with the Wnt pathway.Because the coating effectively accelerates the healing of osteoporotic fractures and improves bone quality,it has significant clinical application prospects.展开更多
基金supported by the National Natural Science Foundation of China[81972058 and 81902194]the Science and Technology Commission of Shanghai Municipality[22YF1422900 and 21002411200]+3 种基金the Shanghai Municipal Key Clinical Specialty,China[shslczdzk06701]Huangpu District Industrial Support Fund[XK2020009]the National Facility for Translational Medicine(Shanghai),China[TMSZ-2020-207]the Shanghai Engineering Research Center of Orthopedic Innovative Instruments and Personalized Medicine Instruments and Personalized Medicine[19DZ2250200].
文摘Osteoporosis results in decreased bone mass and insufficient osteogenic function.Existing titanium alloy implants have insufficient osteoinductivity and delayed/incomplete fracture union can occur when used to treat osteoporotic fractures.Copper ions have good osteogenic activity,but their dose-dependent cytotoxicity limits their clinical use for bone implants.In this study,titanium alloy implants functionalized with a TiCu/TiCuN coating by arc ion plating achieved a controlled release of copper ions in vitro for 28 days.The coated alloy was co-cultured with bone marrow mesenchymal stem cells and showed excellent biocompatibility and osteoinductivity in vitro.A further exploration of the underlying mechanism by quantitative real-time polymerase chain reaction and western blotting revealed that the enhancement effects are related to the upregulation of genes and proteins(such as axin2,β-catenin,GSK-3β,p-GSK-3β,LEF1 and TCF1/TCF7)involved in the Wnt/β-catenin pathway.In vivo experiments showed that the TiCu/TiCuN coating significantly promoted osteoporotic fracture healing in a rat femur fracture model,and has good in vivo biocompatibility based on various staining results.Our study confirmed that TiCu/TiCuN-coated Ti promotes osteoporotic fracture healing associated with the Wnt pathway.Because the coating effectively accelerates the healing of osteoporotic fractures and improves bone quality,it has significant clinical application prospects.
