Low-Temperature Synthesis of Nano-AlN Based on Solid Nitrogen Source by Plasma-Assisted Ball Milling

Plasma-assisted ball milling was carried out on the Al+C3H6N6 system and Al+C_(4)H_(4)N_(4) system,respectively.The phase structure,functional groups and synthesis mechanism were analyzed by XRD and FT-IR,and the differences in the synthesis process of nano-AlN with different solid nitrogen sources were discussed.The results show that C3H6N6 has a stable triazine ring structure,and its chemical bond is firm and difficult to break,so AlN cannot be synthesized directly by solid-solid reaction at room temperature.However,there are a large number of nitrile groups(-CN)and amino groups(-NH_(2))in C_(4)H_(4)N_(4) molecules.Under the combined action of plasma bombardment and mechanical energy activation,C_(4)H_(4)N_(4) molecules undergo polycondensation and deamination,so that the ball milling tank is filled with a large number of active nitrogen-containing groups such as N=,≡N,etc.These groups and ball milling activated Al can synthesize nano-AlN at room temperature,with a conversion rate of 92%.SEM,DSC/TG analysis showed that the powder obtained by ball milling was formed by soft agglomeration of many fine primary particles about 50–80 nm.The surface morphology of the powder was loose and porous,and it had strong activity.After annealing at 800℃,the conversion rate of the Al+C_(4)H_(4)N_(4) system reached 99%.

The pH change in rhizosphere of Pinus koraiensis seedlings as af-fected by different nitrogen sources and its effect on phosphorus availability

Root mat method described by Kuchenbuch and Jungk was used to study the rhizosphere processes. The experi-ment was carried out on two years old Pinus koraiensis seedlings. Soil samples collected from the upper 20-cm soil layer in Changbai Mountain were treated with three different forms of nitrogen fertilizers: NO3--N, NH4+-N and NH4NO3. The results showed that the soil pH and available P near the roots were all lower than in the bulk soil in control treatment. NH4+-N applica-tion greatly decreased the soil pH near the roots compared to the control treatment and promoted the absorption of phosphorus, which led to a more remarkable depletion region of available P. On the contrary, the rhizosphere soil pH was higher than in the bulk soil in treatments with NO3--N and retarded the P absorption, which led to a nearly equal available P contents to the bulk soil. In treatment with NH4NO3, the rhizosphere soil pH was only a little lower than that in the control treatment and its effects on P absorption is mediate between the treatments with NH4+-N and NO3--N.

The Effects of Three Mineral Nitrogen Sources and Zinc on Maize and Wheat Straw Decomposition and Soil Organic Carbon

The incorporation of straw in cultivated ifelds can potentially improve soil quality and crop yield. However, the presence of recalcitrant carbon compounds in straw slow its decomposition rate. The objective of this study was to determine the effects of different nitrogen sources, with and without the application of zinc, on straw decomposition and soil quality. Soils were treated with three different nitrogen sources, with and without zinc： urea （CO（NH2）2）, ammonium sulfate （（NH4）2SO4）, and ammonium chloride （NH4Cl）. The combined treatments were as follows：maize （M） and wheat （W） straw incorporated into urea-, ammonium sulfate-, or ammonium chloride-treated soil （U, S, and C, respectively） with and without zinc （Z） （MU, MUZ, WU, WUZ;MS, MSZ, WS, WSZ;MC, MCZ, WC, WCZ, respectively）;straw with zinc only （MZ, WZ）;straw with untreated soil （MS, WS）;and soil-only or control conditions （NT）. The experiment consisted of 17 treatments with four replications. Each pot contained 150 g soil and 1.125 g straw, had a moisture content of 80%of the ifeld capacity, and was incubated for 53 days at 25°C. The rates of CO2-C emission, cumulative CO2-C evolution, total CO2 production in the soils of different treatments were measured to infer decomposition rates. The total organic carbon （TOC）, labile organic carbon （LOC）, and soil microbial biomass in the soils of different treatments were measured to infer soil quality. All results were signiifcantly different （P〈0.05） with the exception of the labile organic carbon （LOC）. The maize and wheat straw showed different patterns in CO2 evolution rates. For both straw types, Zn had a synergic effect with U, but an antagonistic effect with the other N sources as determined by the total CO2 produced. The MUZ treatment showed the highest decomposition rate and cumulative CO2 concentration （1 120.29 mg/pot）, whereas the WACZ treatment had the lowest cumulative CO2 concentration （1 040.57 mg/pot）. The addition of NH4Cl resulted in the highest total organic carbon （TOC） concentration （11.59 mg kg-1）. The incorporation of wheat straw resulted in higher microbial biomass accumulation in soils relative to that of the maize straw application. The results demonstrate that mineral N sources can affect the ability of microorganisms to decompose straw, as well as the soil carbon concentrations.

Studies on optimization of nitrogen sources for astaxanthin production by Phaffia rhodozyma

Fermentation of Phaffia rhodozyma is a major method for producing astaxanthin, an important pigment with industrial and pharmaceutical application. To improve astaxanthin productivity, single factor and mixture design experiments were used to investigate the effects of nitrogen source on Phaffia rhodozyma cultivation and astaxanthin production. Results of single factor experiments showed nitrogen source could significantly affect P. rhodozyma cultivation with respect to carbon source utilization, yeast growth and astaxanthin accumulation. Further studies of mixture design experiments using (NH4)2SO4, KNO3 and beef extract as nitrogen sources indicated that the proportion of three nitrogen sources was very important to astaxanthin production. Validation experiments showed that the optimal nitrogen source was composed of 0.28 g/L (NH4)2SO4, 0.49 g/L KNO3 and 1.19 g/L beef extract. The kinetic characteristics of batch cultivation were investigated in a 5-L pH-stat fermentor. The maximum amount of biomass and highest astaxanthin yield in terms of volume and in terms of biomass were 7.71 mg/L and 1.00 mg/g, respectively.

Effects of Different Nitrogen Sources and Metal Ions on Biogas Generation by Corn Stalk Fermentation

[Objective] The present study was conducted to explore the effects of dif- ferent nitrogen sources and metal ions on the biogas production process and biogas yield by fermentation of corn stalk powder using self-designed anaerobic fermenta- tion system. [Method] The biogas produced during fermentation was collected by water displacement, and measured by ignition. [Result] Ammonium bicarbonate, urea and yeast extract powder all increased biogas yield. Among them, ammonium bi- carbonate was the best nitrogen source. Ammonium dihydrogen phosphate showed an inhibitory effect on biogas production. Biogas yield was significantly increased by adding NaCl, KCl, MgCl2 or CaCl2, among which, NaCl had the best effect. [Conclu- sion] It is not conducive to the growth and development of methanogenic flora in the production of biogas using corn stalks as the only material in fermentation. The biogas yield can be increased by adding exogenous nitrogen, which reduces the carbon to nitrogen （C：N） ratio of fermentation materials. The biogas yield can be significantly increased by adding appropriate types and amount of electrolytes, with appropriate C：N ratio. The methanogenic flora is more tolerant to NaCl and KCl than to MgCl2 and CaCl2. Moreover, NaCl can not only significantly increase the biogas production, but also shorten startup period of methanogenic flora, thereby shortening the gas producing cycle.

Preliminary Study on the Responses of Three Marine Algae,Ulva pertusa(Chlorophyta),Gelidium amansii(Rhodophyta)and Sargassum enerve(Phaeophyta),to Nitrogen Source and Its Availability

An experiment was designed to select economically valuable macroalga species with high nutrient uptake rates. Such species cultured on a large scale could be a potential solution to eutrophication. Three macroalgae species, Ulva pertusa (Chlorophyta), Gelidium amansii (Rhodophyta) and Sargassum enerve (Phaeophyta), were chosen for the experiment because of their economic values and availability. Control and four nitrogen concentrations were achieved by adding NH + 4 and NO - 3. The results indicate that the fresh weights of all species increase faster than that of control after 5 d culture. The fresh weight of Ulva pertusa increases fastest among the 3 species. However, different species show different responses to nitrogen source and its availability. They also show the advantage of using NH + 4 than using NO - 3. U. pertusa grows best and shows higher capability of removing nitrogen at 200 μmol L -1 , but it has lower economical value. G. amansii has higher economical value but lower capability of removing nitrogen at 200 μmol L -1 . The capability of nitrogen assimilation of S. enerve is higher than that of G. amansii at 200 μmol L -1 , but the former’s increase of fresh weight is lower than those of other two species. Then present preliminary study demonstrates that it is possible to use macroalgae as biofilters and further development of this approach could provide biologically valuable information on the source, fate, and transport of N in marine ecosystems. Caution is needed should we extrapolate these findings to natural environments.

Effect of Nitrogen Sources on the Growth and Docosahexaenoic Acid Accumulation in Crypthecodinium cohnii

The effect of various nitrogen sources on the growth and docosahexaenoic acid (DHA) yield is determined in Crypthecodinium cohnii ATCC30556. Single nitrogen tryptone and peptone are suitable to growth, the dry weight biomass is up to 2.78 g/L and 2.70g/L respectively on medium containing 0.34 g/L nitrogen. Peptone is a favorable nitrogen source for DHA accumulation, DHA yield increases up to 338.56 mg/L. Using peptone and KNO3 as a multiple nitrogen source, the highest biomass and DHA yield are obtained from media containing 0.34 g/L nitrogen in which the ratio of peptone-N:NO3--N is 1:2,and the biomass and DHA yield are 2.98 g/L(DW) and 527.97mg/L respectively.

Influence comparison of N2 and NH3 nitrogen sources on AlN films grown by halide vapor phase epitaxy

A comparison of the nitrogen sources(N2 and NH3)influence on AlN films grown by high-temperature halide vapor phase epitaxy(HVPE)is reported.The x-ray rocking curves(XRCs)indicate that the full width at half maximum(FWHM)of(0002)plane for AlN films using N2 as nitrogen source is generally smaller than that using NH3.Optical microscope and atomic force microscope(AFM)results show that it is presently still more difficult to control the crack and surface morphology of AlN films with thicknesses of 5-10µm using N2 as the nitrogen source compared to that using NH3.Compared with one-step growth,two-step growth strategy has been proved more effective in stress control and reducing the density of threading dislocations for AlN epilayers using N2 as the nitrogen source.These investigations reveal that using N2 as nitrogen source in HVPE growth of AlN is immature at present,but exhibits great potential.

Non-Point-Source Nitrogen and Phosphorus Loadings from a Small Watershed in the Three Gorges Reservoir Area

Non-point-source pollution has become a major threat to the water quality of the Three Gorges Reservoir(TGR);however,nutrient loadings from terrestrial sources are unclear due to a lack of in situ monitoring.A representative small watershed in the central part of the TGR area was selected to monitor the loss of nitrogen(N) and phosphorous(P) continuously along with the runoff from 2007 through 2009 to understand the exact sources and loadings.Results show that the non-point-source nitrogen and phosphorus comes mainly from the storm runoff from residential areas,citrus orchards and sloping croplands,which contributes up to 76% of the loadings in this watershed.Thus,a crucial measure for controlling non-point-source pollution is to intercept storm runoff from the three sources.Paddy fields provide a sink for non-point-source N and P by intercepting the runoff and sediment along with their different forms of nitrogen and phosphorus.The N and P removal efficiency by paddy fields from residential areas is within the range of 56% to 98%.Paddy fields are an important land-use option for reducing the non-point-source loading of N and P in the TGR area.

Influence of nitrogen sources on growth and mycotoxin production by isolates of Pyrenophora tritici-repentis from wheat

The fungus Pyrenophora tritici-repentis(Died.) Drechs. infects the leaves and kernels of wheat,causing tan spot and red smudge, respectively. Isolates of P. tritici-repentis have been reported to be both phytopathogenic and mycotoxigenic. This research investigates the influence of nitrogen sources on growth and production of mycotoxins by eight different isolates of P. tritici-repentis. A synthetic agar medium(SAM) was used with different nitrogen sources, both inorganic [(NH4Cl, NH4NO3and(NH4)2SO4)] and organic(L-alanine, L-histidine, and L-lysine), at a concentration of 37.5 mmol L-1. Individual isolates exhibited different growth rates that varied according to the nitrogen source added to the medium. The choice of nitrogen source also had a major effect on production of the mycotoxins emodin, catenarin and islandicin. The highest concentrations of emodin, 54.40 ± 4.46 μg g-1, 43.07 ± 23.39 μg g-1and28.91 ± 4.64 μg g-1of growth medium, were produced on the complex medium(V8-potato dextrose agar) by the isolates Alg-H2, 331-2 and TS93-71 B, respectively. A relatively high concentration of emodin also was produced by isolates Az35-5(28.29 ± 4.71 μg g-1of medium)and TS93-71B(27.03 ± 4.09 μg g-1of medium) on synthetic medium supplemented with L-alanine. The highest concentrations of catenarin(174.54 ± 14.46 μg g-1and 104.87 ±6.13 μg g-1of medium) were recorded for isolates TS93-71 B and Alg-H2 on synthetic medium supplemented with L-alanine and NH4 Cl, respectively. The highest concentration of islandicin(4.64 ± 0.36 μg g-1medium) was observed for isolate 331-2 in the presence of L-lysine. There was not a close relationship between mycelial growth and mycotoxin production by the fungal isolates. This is the first report on the influence of nitrogen sources on the production of mycotoxins by P. tritici-repentis.

A NEW FACILE METHOD FOR THE SYNTHESIS OF AZACROWN ETHERS BY USING BENZYLSULFONAMIDE AS A SOURCE OF NITROGEN

Benzylsulfonamide was used as a source of nitrogen in the syn- thesis of azacrown ethers,and the cyclic intermediates were easily deben- zylsulfonylated by treating with CaF_2/H_2SO_4 to afford the azacrown ethers.

Effects of Carbon and Nitrogen Additions on Soil Microbial Biomass Carbon and Enzyme Activities Under Rice Straw Returning

The effects of different amounts of carbon and nitrogen sources on the soil microbial biomass carbon,dissolved organic carbon and related enzyme activities were studied by the simulation experiment of rice straw returning to the field,and the mechanism of the decomposition of rice straw returning to the field was discussed.Completely randomized experiment of the two factors of the three levels was designed,and a total of nine treatments of indoor soil incubation tests were conducted.Full amount of rice straw was applied to the soil in this simulation experiment and different amounts of brown sugar and urea were added in the three levels of 0(no carbon source and nitrogen source),1(low levels of carbon and nitrogen sources)and 2(high levels of carbon and nitrogen sources),respectively.The results showed that the addition of different amounts of carbon and nitrogen sources to the rice straw could increase the soil carbon content.Compared with T0N0,the microbial biomass carbon of T2N2 was increased significantly by 170.48%;the dissolved organic carbon content of T1N2 was significantly increased by 58.14%and the free humic acid carbon contents of T0N2,T1N1 and T2N0 were significantly increased by 56.16%and 45.55%and 47.80%,respectively;however,there were no significant differences among those of treatments at later incubation periods.The addition of different carbon and nitrogen sources could promote the soil enzyme activities.During the incubation period,all of the soil enzyme activities of adding sugar and urea were higher than those of T0N0 treatment.Therefore,the addition of different amounts of carbon and nitrogen sources to rice straw returning could improve soil microbial biomass carbon content,dissolved organic carbon and soil enzyme activities.

Influences of dietary protein sources and crude protein levels on intracellular free amino acid profile in the longissimus dorsi muscle of finishing gilts

Background： The current study was carried out to determine effects of dietary protein source and crude protein（CP）level on carcass characteristics, meat quality, and muscle amino acid（AA） profile in finishing gilts. The experiment was designed as a 2 × 2 factorial arrangement with two sources of dietary proteins（cottonseed meal, CSM vs. soybean meal, SBM） and two levels of CP（12 % vs. 14 %, as-fed basis）. Seventy-two crossbred gilts（89.5 ± 0.9 kg） were allotted to one of four dietary treatments in a randomized complete block design for a period of 28 d. All diets were formulated to be isoenergetic and similar concentrations of standardized ileal digestible essential AA covering the nutrient requirements of pigs.Results： Growth, carcass characteristics and meat quality were not affected by dietary protein source nor crude protein level（P ＆gt; 0.10） except that average daily feed intake was increased by CSM diets（P = 0.03）. Gilts offered reduced protein diets had lower muscle p H45min（P 〈 0.05）. Neither dietary protein source nor crude protein level influenced N deposition. However, reduced protein diets decreased N intake, N excretion, and serum urea nitrogen content, whilst improved N efficiency（P 〈 0.01）. CSM diets increased N intake（P = 0.04）,but did not depress N efficiency. The concentrations of phenylalanine, tryptophan, cysteine and tyrosine（P 〈 0.05） of the longissimus muscle were decreased when gilts offered CSM diets, while muscle intracellular free valine concentration was increased（P = 0.03）. The gilts offered reduced protein diets had greater intracellular concentrations of free methionine, lysine, and total AA in muscle（P 〈 0.05）.Conclusion： These results suggest that CSM could replace SBM as a primary protein source in finishing pig diets in terms of performance, N efficiency, carcass characteristics, and meat quality, but decrease the concentrations of muscle specific AA. Furthermore, the reduced protein diet played an important role in increasing muscle intracellular concentrations of specific free amino acids（FAA）, and in reducing the relative ratios of specific FAA to lysine in longissimus dorsi muscle of pig, whose biological meaning needs further studies.

Effects of Medium Nutrients on Fusarium oxysporum Schl. f.sp

[Objective] To study the effects of different culture conditions on the Fusarium oxysporurn SchL f. sp. [Method] Based on species identification of the pathogenic organism of Fusarium oxysporum Schl. f. sp, effects of different cultures and different nutrients on the mycelial growth and conidial production of Fusarium oxysporum SchL f. sp were studied. [Result] The mycelial growth and conidial pro- duction of Fusarium oxysporum SchL f. sp was different under different culture con- ditions. PDA medium was the most suitable medium for the mycelial growth and had the highest conidial production; and the mycelial grew the fastest on the medium with maltose as carbon source or peptone as nitrogen source, which also had the highest conidial production. [Conclusion] This study provided experimental basis for the study of Fusarium oxysporum SchL f. sp and also provided theoretical basis for the study and control of Fusarium oxysporum Schl. f. sp.

Optimal Controlling for Fermentation Process of Threonine-producing Strain Overexpressing rhtB Gene

[Objective] The aim was to construct threonine-producing strain M122 overexpressing key gene rhtB for transporter protein to transport threonine to the extracellular, and to investigate effects of different carbon or nitrogen sources and pH values on L-threonine production caused by the recombinant strain. [Method] The fermentation process of L-threonine-producing strain was analyzed by different carbon or nitrogen sources, and carbon or nitrogen sources and pH in fermentation medium were also optimized in this study. [Result] After direct modification, the uti- lization efficiency of threonine-producing strain on nutrients increases. When sucrose was used as carbon source for fermentation, Lothreonine yield via shaking culture was 28.1 g/L When （NH4）2SO4 or yeast powder was used as nitrogen source for fermenta- tion, L-threonine yield was 27.8 and 28.2 g/L respectively, which were all higher than that of other nitrogen sources. The study on optimal pH value showed that it was more beneficial for strain growth and L-threonine yield on neutral condition. [Conclusion] The best carbon source for threonine-produing strain M122 was sucrose, while the best nitrogen source was （NH4）2804 or yeast powder, and the most suit- able pH value was 7.0.

Characteristics and Culture Conditions of a Bioflocculant Produced by Penicillium sp.

Objective To study the characteristics of a bioflocculant named MBF7 produced by Penicillum strain HHE-P7 and the effects of cultivation conditions on bioflocculant production. Methods The chemical group in the bioflocculant molecules was shown by Fourier transform infrared （FTIR） spectra, and the average molecular weight of MBF7 was estimated by gel permeation chromatography. The effects of medium components on bioflocculant production and flocculating activity were studied. Results Phospho-, amino-, hydroxyl, and carboxyl groups were the major fractions of MBF7, and the molecule weight was about 3.0 × 10^5 Da. In addition, the carbon and nitrogen sources favorable for the bioflocculant production were glucose and yeast extract respectively. When the initial pH of the medium was adjusted to 5.0, high flocculant efficiency could be achieved. Conclusion The bioflocculant MBF7 is a new macromolecule with high flocculating efficiency for Kaolin suspension, and could be produced under appropriate culture conditions.

Enhancement of Laccase Activity by Marine-derived Deuteromycete Pestalotiopsis sp. J63 with Agricultural Residues and Inducers

Pestalotiopsis sp. J63, producing a high activity of laccase, is a new marine-derived fungus isolated from the oceanic sediment of the East China Sea. Since the marine environment is oligotrophic nutrient, marine derived fungi may use small amount of nutrients to grow and produce laccases. Agricultural residues that are mainly composed of lignin, cellulose and hemicellulose are difficult to be degraded and few microbes can take them as substrates, so they are considered as oligotrophic nutrient and have the potential to be used to produce value added products. In this study, the ability of Pestalotiopsis sp. J63 to use agricultural residues to produce laccases was tested in the submerged fermentation. The combination of 3 g·L 1maltose and 20 g·L 1rice straw was the best carbon sources and 8 g·L 1ammonium sulfate was the best nitrogen source under the condition without inducers. The effects of five inducers, the feeding time and concentration of inducer on laccase production were investigated.Adding 0.09 mmol·L 1phenol after 24 h of incubation led to high laccase activity(5089 U·L 1), while with 0.09mmol·L 1phenol in the medium and wheat bran as the nitrogen source, the laccase activity could reach 5791.7U·L 1. Native-PAGE results showed that two laccase isozymes were present in the cultures. One existed in both induced and non-induced culture filtrates, while the other was only found in the fermentation with the addition of phenol, guaiacol and veratryl alcohol.

Coupled effects of methane monooxygenase and nitrogen source on growth and poly-β-hydroxybutyrate(PHB)production of Methylosinus trichosporium OB3b

The coupled effects of nitrogen source and methane monooxygenase（MMO） on the growth and poly-β-hydroxybutyrate（PHB） accumulation capacity of methanotrophs were explored.The ammonia-supplied methanotrophs expressing soluble MMO（s MMO） grew at the highest rate, while N2-fixing bacteria expressing particulate MMO（p MMO） grew at the lowest rate. Further study showed that more hydroxylamine and nitrite was formed by ammonia-supplied bacteria containing p MMO, which might cause their slightly lower growth rate. The highest PHB content（51.0%） was obtained under nitrogen-limiting conditions with the inoculation of nitrate-supplied bacteria containing p MMO. Ammoniasupplied bacteria also accumulated a higher content of PHB（45.2%） with the expression of p MMO, while N2-fixing bacteria containing p MMO only showed low PHB production capacity（32.1%）. The maximal PHB contents of bacteria expressing s MMO were low, with no significant change under different nitrogen source conditions. The low MMO activity,low cell growth rate and low PHB production capacity of methanotrophs continuously cultivated with N2 with the expression of p MMO were greatly improved in the cyclic NO3-N2 cultivation regime, indicating that long-term deficiency of nitrogen sources was detrimental to the activity of methanotrophs expressing pMMO.

Transition from a maternal to external nitrogen source in maize seedlings

Maximizing NO3 uptake during seedling development is important as it has a major influence on plant growth and yield. However, little is known about the processes leading to, and involved in, the initiation of root NO3 uptake capacity in developing seedlings. This study examines the physiological processes involved in root NO3 uptake and metabolism, to gain an understanding of how the NO3 uptake system responds to meet demand as maize seedlings transition from seed N use to external N capture. The concentrations of seedderived free amino acids within root and shoot tissues are initially high, but decrease rapidly until stabilizing eight days after imbibition （DAI）. Similarly, shoot N% decreases, but does not stabilize until 12-13 DAI. Following the decrease in free amino acid concentrations, root NO3- uptake capacity increases until shoot N% stabilizes. The increase in root NO3 uptake capacity corresponds with a rapid rise in transcript levels of putative NO3 transporters, ZmNRT2.1 and ZmNRT2.2. The processes underlying the increase in root NO3- uptake capacity to meet N demand provide an insight into the processes controlling N uptake.

Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5

The effects of different NaCl concentrations, nitrogen sources, carbon sources, and carbon to nitrogen molar ratios on biomass accumulation and polyhydroxybutyrate （PHB） production were studied in batch cultures of the marine photosynthetic bacterium Rhodovulum sulfidophilum P5 under aerobic-dark conditions. The results show that the accumulation of PHB in strain P5 is a growth-associated process. Strain P5 had maximum biomass and PHB accumulation at 2%-3% NaCl, suggesting that the bacterium can maintain growth and potentially produce PHB at natural seawater salinity. In the nitrogen source test, the maximum biomass accumulation （8.10±0.09 g/L） and PHB production （1.11±0.13 g/L and 14.62%±2.25% of the cell dry weight） were observed when peptone and ammonium chloride were used as the sole nitrogen source. NH^-N was better for PHB production than other nitrogen sources. In the carbon source test, the maximum biomass concentration （7.65±0.05 g/L） was obtained with malic acid as the sole carbon source, whereas the maximum yield of PHB （5.03±0.18 g/L and 66.93%± 1.69% of the cell dry weight） was obtained with sodium pyruvate as the sole carbon source. In the carbon to nitrogen ratios test, sodium pyruvate and ammonium chloride were selected as the carbon and nitrogen sources, respectively. The best carbon to nitrogen molar ratio for biomass accumulation （8.77±0.58 g/L） and PHB production （6.07±0.25 g/L and 69.25%±2.05% of the cell dry weight） was 25. The results provide valuable data on the production of PHB by R. sulfidophilum P5 and further studies are on-going for best cell growth and PHB yield.