Seasonal response of nitrogen exchange fluxes to crab disturbance at sediment-water interface in coastal tidal wetlands

Coastal wetlands are hotspots for nitrogen(N)cycling,and crab burrowing is known to transform N in intertidal marsh soils.However,the underlying mechanisms remain unclear.This study conducted field experiments and used indoor control test devices to investigate the seasonal response of nitrogen to crab disturbance at the sediment-water interface in coastal tidal flat wetlands.The results showed that crab disturbance exhibited significant seasonality with large seasonal differences in cave density and depth.Due to crab disturbance,nitrogen fuxes at the sediment-water interface were much greater in the box with crabs than in the box without crabs.In summer,NH-N showed a positive flux from the sediment to the overlying water,but NO2-N and NOg-N showed positive fluxes from the sediment to the overlying water only in early stages.In winter,NH-N showed a positive flux from the sediment to the overlying water,but NO-N and NO,-N both exhibited positive and negative fluxes.These results indicated that the presence of crab burrows can cause the aerobic layer to move downward by approximately 8-15 cm in summer and directly promote nitrification at the sediment surface.

Tracing nitrate sources in one of the world's largest eutrophicated bays(Hangzhou Bay):insights from nitrogen and oxygen isotopes

Eutrophication caused by inputs of excess nitrogen(N) has become a serious environmental problem in Hangzhou Bay(China),but the sources of this nitrogen are not well understood.In this study,the August 2019 distributions of salinity,nutrients [nitrate(NO_(3)^(-)),nitrite,ammonium,and phosphate],and the stable isotopic composition of NO_(3)^(-)(δ^(15)N and δ^(18)O) were used to investigate sources of dissolved inorganic nitrogen(DIN) to Hangzhou B ay.Spatial distributions of nitrate,salinity,and nitrate δ^(18)O indicate that the Qiantang River,the Changjiang River,and nearshore coastal waters may all contribute nitrate to the bay.Based on the isotopic compositions of nitrate in these potential source waters and conservative mixing of nitrate in our study area,we suggest that the NO_(3)^(- )in Hangzhou B ay was likely derived mainly from soils,synthetic N fertilizer,and manure and sewage.End-member modeling indicates that in the upper half of the bay,the Qiantang River was a very important DIN source,possibly contributing more than 50% of DIN in the bay head area.In the lower half of the bay,DIN was sourced mainly from strongly intruding coastal water.DIN coming directly from the Changjiang River made a relatively small contribution to Hangzhou Bay DIN in August 2019.

Atmospheric nitrogen deposition affects forest plant and soil system carbon:nitrogen:phosphorus stoichiometric flexibility:A meta-analysis

Background:Nitrogen(N)deposition affects forest stoichiometric flexibility through changing soil nutrient availability to influence plant uptake.However,the effect of N deposition on the flexibility of carbon(C),N,and phosphorus(P)in forest plant-soil-microbe systems remains unclear.Methods:We conducted a meta-analysis based on 751 pairs of observations to evaluate the responses of plant,soil and microbial biomass C,N and P nutrients and stoichiometry to N addition in different N intensity(050,50–100,>100 kg·ha^(-1)·year^(-1)of N),duration(0–5,>5 year),method(understory,canopy),and matter(ammonium N,nitrate N,organic N,mixed N).Results:N addition significantly increased plant N:P(leaf:14.98%,root:13.29%),plant C:P(leaf:6.8%,root:25.44%),soil N:P(13.94%),soil C:P(10.86%),microbial biomass N:P(23.58%),microbial biomass C:P(12.62%),but reduced plant C:N(leaf:6.49%,root:9.02%).Furthermore,plant C:N:P stoichiometry changed significantly under short-term N inputs,while soil and microorganisms changed drastically under high N addition.Canopy N addition primarily affected plant C:N:P stoichiometry through altering plant N content,while understory N inputs altered more by influencing soil C and P content.Organic N significantly influenced plant and soil C:N and C:P,while ammonia N changed plant N:P.Plant C:P and soil C:N were strongly correlated with mean annual precipitation(MAT),and the C:N:P stoichiometric flexibility in soil and plant under N addition connected with soil depth.Besides,N addition decoupled the correlations between soil microorganisms and the plant.Conclusions:N addition significantly increased the C:P and N:P in soil,plant,and microbial biomass,reducing plant C:N,and aggravated forest P limitations.Significantly,these impacts were contingent on climate types,soil layers,and N input forms.The findings enhance our comprehension of the plant-soil system nutrient cycling mechanisms in forest ecosystems and plant strategy responses to N deposition.

Refining the Factors Affecting N_(2)O Emissions from Upland Soils with and without Nitrogen Fertilizer Application at a Global Scale

Nitrous oxide(N_(2)O)is a long-lived greenhouse gas that mainly originates from agricultural soils.More and more studies have explored the sources,influencing factors and effective mitigation measures of N_(2)O in recent decades.However,the hierarchy of factors influencing N_(2)O emissions from agricultural soils at the global scale remains unclear.In this study,we carry out correlation and structural equation modeling analysis on a global N_(2)O emission dataset to explore the hierarchy of influencing factors affecting N_(2)O emissions from the nitrogen(N)and non-N fertilized upland farming systems,in terms of climatic factors,soil properties,and agricultural practices.Our results show that the average N_(2)O emission intensity in the N fertilized soils(17.83 g N ha^(-1)d^(-1))was significantly greater than that in the non-N fertilized soils(5.34 g N ha^(−1) d^(−1))(p<0.001).Climate factors and agricultural practices are the most important influencing factors on N_(2)O emission in non-N and N fertilized upland soils,respectively.For different climatic zones,without fertilizer,the primary influence factors on soil N_(2)O emissions are soil physical properties in subtropical monsoon zone,whereas climatic factors are key in the temperate zones.With fertilizer,the primary influence factors for subtropical monsoon and temperate continental zones are soil physical properties,while agricultural measures are the main factors in the temperate monsoon zone.Deploying enhanced agricultural practices,such as reduced N fertilizer rate combined with the addition of nitrification and urease inhibitors can potentially mitigate N_(2)O emissions by more than 60%in upland farming systems.

Review of study on mineralization, saturation and cycle of Nitrogen in forest ecosystems

Nitrogen is one of the most important elements that can limit plant growth in forest ecosystems. Studies of nitrogen mineralization, nitrogen saturation and nitrogen cycle in forest ecosystems is very necessary for understanding the productivity of stand, nutrient cycle and turnover of nitrogen of forest ecosystems. Based on comparison and analysis of domestic and in-ternational academic references related to studies on nitrogen mineralization, nitrogen saturation and nitrogen cycle in recent 10 years, the current situation and development of the study on these aspects, and the problems existed in current researches were reviewed. At last, some advices were given for future researches.

Numerical Study of Impacts of Soil Moisture on the Diurnal and Seasonal Cycles of Sensible/Latent Heat Fluxes over Semi-arid Region

The semi-arid regions, as climatic and ecosystem transitional zones, are the most vulnerable to global environmental change. Earlier researches indicate that the semi-arid regions are characterized by strong landatmosphere coupling in which soil moisture is the crucial variable in land surface processes. In this paper, we investigate the sensitivity of the sensible/latent heat fluxes to soil moisture during the growing season based on the enhanced observations at Tongyu in the Jilin province of China, a reference site of international Coordinated Energy and Water Cycle Observations Project （CEOP） in the semi-arid regions, by using a sophisticated land surface model （NCAR_CLM3.0）. Comparisons between the observed and simulated sensible/latent heat fluxes indicate that the soil moisture has obvious effects on the sensible/latent heat fluxes in terms of diurnal cycle and seasonal evolution. Better representation of the soil moisture could improve the model performance to a large degree. Therefore, for the purpose of simulating the land-atmosphere interaction and predicting the climate and water resource changes in semi-arid regions, it is necessary to enhance the description of the soil moisture distribution both in the way of observation and its treatment in land surface models.

Nitrogen Biological Cycle Characteristics of Seepweed(Suaeda salsa) Wetland in Intertidal Zone of Huanghe(Yellow) River Estuary

From April 2008 to November 2009, the nitrogen （N） cycle of plant-soil system in seepweed （Suaeda salsa） wetland in the intertidal zone of the Huanghe （Yellow） River estuary was studied. Results showed that soil N had sig- nificant seasonal fluctuations and vertical distribution, and the net N mineralization rates in topsoil were significantly different in growing season （p 〈 0.01）. The N/P ratio （9.87 ±1.23） of S. salsa was less than 14, indicating that plant growth was limited by N. The N accumulated in S. salsa litter at all times during decomposition, which was ascribed to the N immobilization by microbes from the environment. Soil organic N was the main N stock of plant-soil system, accounting for 97.35% of the total N stock. The N absorption and utilization coefficients of S. salsa were very low （0.0145 and 0.3844, respectively）, while the N cycle coefficient was high （0.7108）. The results of the N turnovers among compartments of S. salsa wetland showed that the N uptake amount of aboveground part and root were 7.764 g/m2and 4.332 g/m2, respectively. The N translocation amounts from aboveground part to root and from root to soil were 3.881 g/m2 and 0.626 g/m2, respectively. The N translocation amount from aboveground living body to litter was 3.883 g/m2, the annual N return amount from litter to soil was more than 0.125（-） g/m2 （minus represented immobili- zation）, and the net N mineralization amount in topsoil （0-15 cm） in growing season was 1.190 g/m2. The assessment of N biological cycle status orS. salsa wetland indicated that N was a very important limiting factor and the ecosystem was situated in unstable and vulnerable status. The S. salsa was seemingly well adapted to the low-nutrient status and vulnerable habitat, and the N quantitative relationships determined in the compartment model might provide scientific base for us to reveal the special adaptive strategy orS. salsa to the vulnerable habitat in the following studies.

Application of Stable Isotope Techniques in Studies of Carbon and Nitrogen Biogeochemical Cycles of Ecosystem

Stable isotope techniques have been proved useful as tools for studying the carbon (C) and nitrogen (N) biogeochemical cycles of ecosystem. This paper firstly introduced the basic principles and the distribution characteristics of stable isotope, then reviewed the recent advances and applications of stable isotope in the C and N biogeochemical cycles of ecosystem. By applying the 13 C natural abundance technique, ecologists are able to understand the photosynthetic path and CO 2 fixation of plants, the CO 2 exchange and C balance status of ecosystem, the composition, distribution and turnover of soil organic C and the sources of organic matter in food webs, while by using the 13 C labeled technique, the effects of elevated CO 2 on the C processes of ecosystem and the sources and fate of organic matter in ecosystem can be revealed in detail. Differently, by applying the 15 N natural abundance technique, ecologists are able to analyze the biological N 2 -fixation, the N sources of ecosystem, the N transformation processes of ecosystem and the N trophic status in food webs, while by using the 15 N labeled technique, the sources, transformation and fate of N in ecosystem and the effects of N input on the ecosystem can be investigated in depth. The applications of both C and N isotope natural abundance and labeled techniques, combined with the elemental, other isotope ( 34 S) and molecular biomarker information, will be more propitious to the investigation of C and N cycle mechanisms. Finally, this paper concluded the problems existed in current researches, and put forward the perspective of stable isotope techniques in the studies on C and N biogeochemical cycles of ecosystem in the future.

Summary of Recent Climate Change Studies on the Carbon and Nitrogen Cycles in the Terrestrial Ecosystem and Ocean in China

This article reviews recent advances over the past and their relationship to climate change in China. The was 0.19-0.26 Pg C yr-1 for the 1980s and 1990s. 4 years in the study of the carbon-nitrogen cycling net carbon sink in the Chinese terrestrial ecosystem Both natural wetlands and the rice-paddy regions emitted 1.76 Tg and 6.62 Tg of CH4 per year for the periods 1995 2004 and 2005 2009, respectively. China emitted -1.1 Tg N20-N yr-1 to the atmosphere in 2004. Land soil contained -8.3 Pg N. The excess nitrogen stored in farmland of the Yangtze River basin reached 1.51 Tg N and 2.67 Tg N in 1980 and 1990, respectively. The outer Yangtze Estuary served as a moderate or significant sink of atmospheric CO2 except in autumn. Phytoplankton could take up carbon at a rate of 6.4 ×1011 kg yr-1 in the China Sea. The global ocean absorbed anthropogenic CO2 at the rates of 1.64 and 1.73 Pg C yr-1 for two sinmlations in the 1990s. Land net ecosystem production in China would increase until the mid-21st century then would decrease gradually under future climate change scenarios. This research should be strengthened in the future, including collection of more observation data, measurement of the soil organic carbon （SOC） loss and sequestration, evaluation of changes in SOC in deep soil layers, and the impacts of grassland management, carbon-nitrogen coupled effects, and development and improvement of various component models and of the coupled carbon cycle-climate model.

Theoretical study on the ideal open cycle of the liquid nitrogen engine

This article described the characteristics of the liquid nitrogen engine's ideal open cycle.Using two interconnecting strokes to achieve the power output can mitigate the trade-off between high efficiency and the potential mechanical complexity of multiple-cylinder engines. The total specific energy of the binary media (methane-nitrogen) cycle system could be much higher than the unitary medium (liquid nitrogen) cycle system. By theoretical analysis, the reasonably acceptable driving range proved the feasibility of the liquid nitrogen engine used for supplying power for a lightweight car.

Effects and underlying mechanisms of damming on carbon and nitrogen cycles and transport in rivers of Southwest China:project introduction

Southwest China is the primary area for damming rivers to produce hydroelectric energy and store water.River damming has changed hydrodynamic,chemical,and biological processes,which are related to sinks and sources of greenhouse gases and carbon and nitrogen fluxes of different interfaces.Here,I provide an introduction to a river damming-related foundation,the National Key R&D Program of China(2016YTA0601000).Supported by the foundation,we carried out research on multiprocesses/multi-interfaces of carbon and nitrogen biogeochemical cycles in a dammed river system and have produced important results,as presented in this issue of the journal.

Integration of nitrogen dynamics into the land surface model AVIM.Part 2:baseline data and variation of carbon and nitrogen fluxes in China

The spatiotemporal features of carbon and nitrogen fluxes over China between 1979 and 2015were simulated by the Atmosphere–Vegetation Interaction Model(AVIM).The carbon fluxes of gross primary production and net primary production captured the distribution pattern in China better than MODIS and TRENDY data.The results for nitrogen deposition and biological nitrogen fixation show the good performance of the AVIM simulation compared with the CMIP6 and CABLE data,with a deposition rate>4 g N m-2yr-1in south China.The variation in the gross primary production and net primary production can be up to 300 and 200 g C m-2yr-1in south and southeast China,respectively,and there is a discrepancy between the AVIM and the data from MODIS and TRENDY.This shows the difficulty in simulating the carbon flux in a monsoon climate region and the importance of coupling the nitrogen–carbon fluxes.The standard deviation of nitrogen deposition and biological nitrogen fixation is simulated well by the AVIM and there is a large range in nitrogen deposition of 0.8–1.2 g N m-2yr-1in south China.The climatological mean of the fluxes performs better than the variation in the standard deviation and anomaly and this variation in the carbon–nitrogen flux is the key to decreasing bias in future modeling studies.

Nitrogen and Phosphorus Diffusive Fluxes Across the Sediment-Water Interface in Estuarine and Coastal Tidal Flats

Nitrogen and phosphorus contents are analyzed in the overlying waters and pore waters taken from the Changjiang Estuary and Shanghai coastal tidal flats in this study. In addition, the diffusion fluxes of nitrogen and phosphorus across the sediment-water interface in tidal flats are estimated according to the nutrient concentration gradients at the interface. It has been indicated that the concentrations of ammonium, nitrite, nitrate and dissolved phosphorus in overlying waters range from 0.0082-2.56, 0.03-0.58, 0.69-5.38 and 0.035-0.53 mg/L, respectively, while 0.0025 - 1.35 mg /L for NH^-N, 0. 0055 ~0.20mg/L for NO2-N, 0.61-1.14 mg/L for NO3-N and 0.11~0.53mg/L for DP insurface pore waters.The findings have revealed that ammonium, nitrite, nitrate and dissolved phosphorus diffusionfluxes across the sediment-water interface are between -0.024~0.99, -0.39~ -0.0019, -3.09--0.12 and -0.48- 0.12 ug/ (cm.d ) respectively, showing that the sediment in tidal flats is the source of phosphorus and an important sink for nitrogen in the waters.

Simulating Dry Deposition Fluxes of PM_(10) and Particulate Inorganic Nitrogen over the Eastern China Seas During a Severe Asian Dust Event Using WRF-Chem Model

A WRF-Chem model including a comprehensive gas-phase nitrogen chemistry module was used to simulate a severe dust event appearing in the eastern China on 19-25 March, 2002. The modeling result well reproduced PM10 concentrations in various distances from the dust sources and the transport pathway of the dust strom. The results showed that both the concentrations and the dry deposition fluxes of PM10 increased over the China seas during the dust event following the passage of a cold front system. The maximum fluxes of PM10 in the Yellow Sea and the East China Sea during the dust event were 5.5 and 8.4 times of those before the event, respectively. However, the temporal variations of the dry deposition fluxes of particulate inorganic nitrogen differed over the Yellow Sea from those over the East China Sea. Nitrate and ammonium in the whole northern China rapidly decreased because of the intrusion of dust-loaded air on 19 March. The dust plume arrived in the Yellow Sea on 20 March, decreasing the particulate inorganic nitrogen in mass concentration accordingly. The minimum dry deposition fluxes of nitrate and ammonium in the Yellow Sea were about 3/5 and 1/6 of those before the dust arrival, respectively. In contrast, when the dust plume crossed over the Yangtze Delta area, it became abundant in nitrate and ammonium and increased the concentrations and dry deposition fluxes of particulate inorganic nitrogen over the East China Sea, where the maximum dry deposition fluxes of nitrate and ammonium increased approximately by 4.1 and 2.6 times of those prior to the dust arrival.

Responses of soil CH_(4) fluxes to nitrogen addition in two tropical montane rainforests in southern China

Background:Atmospheric nitrogen(N)deposition is projected to increase in the next few decades,which may have a marked impact on soil-atmosphere CH_(4) fluxes.However,the impacts of increased atmospheric N depositions on soil CH_(4) flux in tropical rainforests are still poorly understood.From January 2015 to December 2018,a field experiment was conducted in a primary tropical montane rainforest(PTMR)and a secondary tropical montane rainforest(STMR)in southern China to quantify the impact of N additions at four levels(N0:0 kg N⋅ha^(-1)⋅year^(-1);N25:25 kg N⋅ha^(-1)⋅year^(-1);N50:50 kg N⋅ha^(-1)⋅year^(-1);N100:100 kg N⋅ha^(-1)⋅year^(-1)on soil CH_(4) flux.Results:Four years of measurements showed clear seasonal variations in CH_(4) flux in all treatment plots for both forest types(PTMR and STMR),with lower rates of soil CH_(4) uptake during the wet season and higher rates of soil CH_(4) uptake during the dry season.Soil CH_(4) uptake rates were significantly and negatively correlated with both soil temperature and soil moisture for both forest types.Annual CH_(4) uptake for the N0 plots from the PTMR and STMR soils were2.20 and1.98 kg N⋅ha^(-1)⋅year^(-1),respectively.At the PTMR site,mean CH_(4) uptake compared with the N0 treatment was reduced by 19%,29%,and 36%for the N25,N50,and N100 treatments,respectively.At the STMR site,mean CH_(4) uptake compared with the N0 treatment was reduced by 15%,18%,and 38%for the N25,N50,and N100 treatments,respectively.High level N addition had a stronger inhibitory impact on soil CH_(4) uptake than did the low level N addition.Conclusion:Our data suggest that soil CH_(4) uptake in tropical rainforests is sensitive to N deposition.If atmospheric N deposition continues to increase in the future,the soil CH_(4) sink strength of tropical rainforests may weaken further.

Improvement of Taihu water quality by the technology of immobilized nitrogen cycle bacteria

Experimental studies were carried out on the purification of eutrophicTaihu Lake water by dynamic experiment using immobilized nitrogen cycle bacteria(INCB). The results showed that the eutrophic water of Taihu Lake can be purifiedeffectively as it passes through the experimental reactor into which some immobilizednitrogen cycle bacteria were put. The removal efficiencies for Total N (TN), NH4+-Nwith immobilized nitrogen cycle bacteria were 72.4% and 85.6%, respectively. It wasfound that the immobilized nitrogen cycle bacteria also have purificatory effect oneutrophic water of Taihu Lake at winter temperature (7°C), and that the removalmefficiencies for Total N (TN), NH4+-N were 55.6%, and 58.9%, respectively. Theremoval efficiencies for TN and NH4+-N depend on the time the water stays in theexperimental reactor.

Responses of nutrient biogeochemistry and nitrogen cycle to seasonal upwelling in coastal waters of the eastern Hainan Island

The coastal upwelling has profound influence on the surrounding ecosystem by supplying the nutrient-replete water to the euphotic zone.Nutrient biogeochemistry was investigated in coastal waters of the eastern Hainan Island in summer 2015 and autumn 2016.From perspectives of nutrient dynamics and physical transport,the nutrient fluxes entered the upper 50 m water depth(between the mixed layer and the euphotic zone)arisen from the upwelling were estimated to be 2.5-5.4 mmol/(m^(2)·d),0.15-0.28 mmol/(m^(2)·d),and 2.2-7.2 mmol/(m^(2)·d)for dissolved inorganic nitrogen(DIN),phosphate(DIP),and dissolved silicate(DSi),respectively,which were around 6-to 12-fold those in the background area.The upwelled nutrients supported an additional plankton growth of(14.70±8.95)mg/m^(2)for chlorophyll a(Chl a).The distributions of nitrateδ^(15)N andδ^(18)O above the 300 m water depth(top of the North Pacific Intermediate Water)were different among the upwelling area,background area in summer,and the stations in autumn,and the difference of environmental and biogeochemical conditions between seasons should be the reason.The higher DIN/DIP concentration ratio,nitrate concentration anomaly,and lower nitrate isotope anomaly(Δ(15,18))in the upper ocean in summer than in autumn indicated the stronger nitrogen fixation and atmospheric deposition,and the following fixed nitrogen regeneration in summer.The higher values of Chl a and nitrateδ^(15)N andδ^(18)O within the euphotic zone in autumn than the background area in summer suggested the stronger nitrate assimilation in autumn.The differences in relatively strength of the assimilation,nitrogen fixation and atmospheric deposition,and the following remineralization and nitrification between the two seasons made the higherδ^(18)O:δ^(15)N and larger difference of enzymatic isotope fractionation factors^(15)εand^(18)εfor nitrate assimilation in summer than in autumn above the North Pacific Tropical Water.

Nitrogen cycle model of agroecosystem in the karst region of Guizhou Province

According to the transport theory of soil solutes and the conditions of soil,geology and climate in the karst region of Guizhou Province,a numerical simulation model of edaphic nitrogen element transport under field conditions is initially established.In this model,NO-3 and NH+4 are regarded as soil solutes.Transformation mechanisms such as biological release,bio-immobilization,ammonium adsorption-desorption,nitration-denitrification and factors of crop root uptaking are considered in this model.It is hoped that the data from this model could directly be used to guide agricultural production in this region and offer feasible ways to improve the use of nitrogen element,sustainable development of agriculture in karst mountainous areas and natural environment.

Dynamic pursuing ecological model concerning the nitrogen cycle in the environment of Chinese mainland

The paper gives a special dynamic pursuing ecological model on the bio-geochemical cycle of nitrogen in the whole range 9.6 million square kilometers of Chinese mainland, basing on the reservoir-content and flux-rate of nitrogen and among the four spheres: atmosphere, pedosphere, biosphere, and hydrosphere, and the law of bio-geochemical cycle of nitrogen, the model predicted the size of reservoir capacities and fluxes of nitrogen in each sphere. Through tested and verified, the model was proven reasonable and reliable.

Denitrogenization from Liquid Steel by Fluxes Treatment

By measuring the solubility of nitrogen in BaO-contained and TiO_2-contained fluxes at 1623K, the nitride capacity and nitrogen distribution ratio were calculated. Both fluxes had high nitride capacity and nitrogen distribution ratio. The results indicated that Both fluxes treatment were available for denitrogenizing steel. The kinetic studies about denitrogenization showed that nitrogen transfer in liquid steel is the controlled step of denitrogenization reaction, so to improve the mass transfer condition in liquid steel could accelerate the rate of denitrogenization. Under proper test conditions, it was proved to be possible to remove nitrogen over 70 percent from steel with TiO_2 contained fluxes.