The objectives of this study were to explore the changes in soil stoichiometry and enzyme activities at different distances from an opencast coal mine in the Hulun Buir Grassland of China. Four transects were establis...The objectives of this study were to explore the changes in soil stoichiometry and enzyme activities at different distances from an opencast coal mine in the Hulun Buir Grassland of China. Four transects were established on north and east sides of the opencast coal mining area, and samples were collected at 50 m, 550 m, and 1550 m from the pit on each transect. Control samples were collected from a grassland station 8 km from the opencast coal mining area that was not disturbed by mining. Four replicate soil samples were collected at each point on the four transects. Soil physicochemical properties and enzyme activities were determined, and correlations between soil properties and stoichiometric ratios and enzyme activities were explored using redundancy analysis. The increase in distance from mining did not significantly affect soil properties, although soil urease activity was significantly lower than that of the control area. Soil properties 1550 m from the mine pit were similar to those at the grassland control. In addition, soil total nitrogen had the greatest effect on soil stoichiometry, and soil total potassium had the greatest effect on soil enzyme activities. Coal dust from opencast mining might be the main factor affecting soil stoichiometry and enzyme activities. The results of this study provide direction for the next step in studying the influence of mining areas on soil properties and processes.展开更多
Background:Nitrogen(N)deposition affects forest stoichiometric flexibility through changing soil nutrient availability to influence plant uptake.However,the effect of N deposition on the flexibility of carbon(C),N,and...Background:Nitrogen(N)deposition affects forest stoichiometric flexibility through changing soil nutrient availability to influence plant uptake.However,the effect of N deposition on the flexibility of carbon(C),N,and phosphorus(P)in forest plant-soil-microbe systems remains unclear.Methods:We conducted a meta-analysis based on 751 pairs of observations to evaluate the responses of plant,soil and microbial biomass C,N and P nutrients and stoichiometry to N addition in different N intensity(050,50–100,>100 kg·ha^(-1)·year^(-1)of N),duration(0–5,>5 year),method(understory,canopy),and matter(ammonium N,nitrate N,organic N,mixed N).Results:N addition significantly increased plant N:P(leaf:14.98%,root:13.29%),plant C:P(leaf:6.8%,root:25.44%),soil N:P(13.94%),soil C:P(10.86%),microbial biomass N:P(23.58%),microbial biomass C:P(12.62%),but reduced plant C:N(leaf:6.49%,root:9.02%).Furthermore,plant C:N:P stoichiometry changed significantly under short-term N inputs,while soil and microorganisms changed drastically under high N addition.Canopy N addition primarily affected plant C:N:P stoichiometry through altering plant N content,while understory N inputs altered more by influencing soil C and P content.Organic N significantly influenced plant and soil C:N and C:P,while ammonia N changed plant N:P.Plant C:P and soil C:N were strongly correlated with mean annual precipitation(MAT),and the C:N:P stoichiometric flexibility in soil and plant under N addition connected with soil depth.Besides,N addition decoupled the correlations between soil microorganisms and the plant.Conclusions:N addition significantly increased the C:P and N:P in soil,plant,and microbial biomass,reducing plant C:N,and aggravated forest P limitations.Significantly,these impacts were contingent on climate types,soil layers,and N input forms.The findings enhance our comprehension of the plant-soil system nutrient cycling mechanisms in forest ecosystems and plant strategy responses to N deposition.展开更多
We examined how afforestation patterns impact carbon(C),nitrogen(N),and phosphorus(P)stoichiometry in the plant-litter-soil system.Plant leaf,branch,stem,and root,litter,and soil samples were collected from mixedspeci...We examined how afforestation patterns impact carbon(C),nitrogen(N),and phosphorus(P)stoichiometry in the plant-litter-soil system.Plant leaf,branch,stem,and root,litter,and soil samples were collected from mixedspecies plantations of Robinia pseudoacacia with Amygdalus davidiana(RPAD),R.pseudoacacia with Armeniaca sibirica(RPAS),and monocultures of R.pseudoacacia(RP),A.davidiana(AD),and A.sibirica(AS)in the Loess Hilly Region.The results showed that in mixed-species plantations,R.pseudoacacia had lower leaf N and P concentrations than in monocultures,while both A.davidiana and A.sibirica had higher leaf N and P concentrations.Soil P limited tree growth in both afforestation models.Mixing R.pseudoacacia with A.davidiana or A.sibirica reduced N-limitation during litter decomposition.Average soil total N and P concentrations were higher in RPAS than in RPAD,and both were higher than the corresponding monocultures.The average soil C:N ratio was the smallest in RPAS,while the average soil C:P ratio was larger in RPAS than in RP.A positive correlation between N and P concentrations,and between C:N and C:P ratios,was found in litter and all plant organs of mono-and mixedstands.Alternatively,for N concentration and C:N ratio,the correlations between plant(i.e.,leaf,branch,root)and litter and between plant and soil were inverse between plantation types.RPAD has an increased litter decomposition rate to release N and P,while RPAS has a faster rate of soil N mineralization.RPAD was the best plantation(mixed)to improve biogeochemical cycling,as soil nutrient restrictions,particularly for P-limitation,on trees growth were alleviated.This study thus provides insights into suitable tree selection and management by revealing C:N:P stoichiometry in the plant-litter-soil system under different afforestation patterns.展开更多
Soybean and lentil are important legume crops in southern Saskatchewan (SK) that can supply the majority of their nitrogen (N) requirement through biological N fixation (BNF). However, the onset of BNF can be slow in ...Soybean and lentil are important legume crops in southern Saskatchewan (SK) that can supply the majority of their nitrogen (N) requirement through biological N fixation (BNF). However, the onset of BNF can be slow in cold;dry prairie soils and a small amount of seed-row placed fertilizer containing both N and phosphorus (P) may benefit the crop. Nevertheless, high rates of fertilizer in close proximity to the seed can also cause injury. This study was conducted to determine the response of lentil and soybean to a starter N-P fertilizer blend applied in the seed-row. A farm field located at the boundary of the Brown and Dark Brown soil zones in south-central Saskatchewan was selected to evaluate the effect of seed-row placed N-P fertilizer blend: 50% Urea + 50% mono-ammonium phosphate (MAP) applied at 0, 10, 20 and 30 kg N and P2O5 ha-1 on emergence, yield, and nutrient uptake. The proportion of nitrogen derived from fixation (ndff) was determined in the soybean using N-15 dilution technique. The rate of 10 kg N and P2O5 ha-1 was found to be the rate that did not significantly reduce emergence, stand count or proportion of N derived from fixation, and was sufficient to maximize yield, N and P uptake for both soybean and lentil under field conditions. Rates higher than 10 kg N ha-1 in the seed row as starter 28-26-0 blend reduced emergence and decreased the proportion of ndff.展开更多
Homeostasis is the adaptability of a species to a changing environment.However,the ecological stoichiometric homeostasis of Robinia pseudoacacia L.in diff erent climatic regions is poorly understood but could provide ...Homeostasis is the adaptability of a species to a changing environment.However,the ecological stoichiometric homeostasis of Robinia pseudoacacia L.in diff erent climatic regions is poorly understood but could provide insights into its adaptability in the loess hilly region.This study sampled 20 year-old R.pseudoacacia plantations at 10 sites along a north–south transect on the Loess Plateau.Variations in the ecological stoichiometric characteristics of leaf and soil carbon,nitrogen,and phosphorus were analysed and homeostatic characteristics of leaf ecological stoichiometric parameters in diff erent climates were identifi ed.Factors aff ecting leaf stoichiometry were assessed.The results show that R.pseudoacacia leaves were rich in nitrogen and defi cient in phosphorous during tree growth and development.Nitrogen and phosphorous levels in the soils of the loess region were lower than the average in soils in the rest of China.All ecological stoichiometric parameters of R.pseudoacacia leaves in two diff erent climates were considered“strictly homeostasis”.Precipitation,available phosphorus,and soil C:P were the main factors aff ecting the variation of C:N:P stoichiometry of R.pseudoacacia leaves.R.pseudoacacia in the loess hilly region has strong ecologically homeostatic characteristics and suggests that it is well-adapted to the area.展开更多
Ecological stoichiometry is an important indicator of biogeochemical cycles and nutrient limitations in terrestrial ecosystems.However,little is known about the response of ecological stoichiometry to plant growth.In ...Ecological stoichiometry is an important indicator of biogeochemical cycles and nutrient limitations in terrestrial ecosystems.However,little is known about the response of ecological stoichiometry to plant growth.In this study,carbon(C),nitrogen(N),and phosphorus(P)concentrations were evaluated in plant tissues(trees,shrubs,and herbs),litter,and soil of young(≤40-year-old),middle-aged(41–60-year-old),near-mature(61–80-year-old),and mature(81–120-year-old)Quercus secondary forests on the Loess Plateau,China.Vegetation composition,plant biomass,and C stock were determined to illustrate their interaction with stoichiometry.Only tree biomass C signifi cantly increased with stand development.Leaf N and trunk P concentrationsgenerally increased,but branch P decreased with growth stage.Fine roots had the highest C and P concentrations at the middle-aged stage.In contrast,shrubs,herbs,litter,and soil C:N:P stoichiometry did not change signifi cantly during stand development.Leaf N and P were positively correlated with soil C,N,P,and their ratios.However,there was no signifi cant correlation between litter and leaves in terms of C:N:P stoichiometry.A redundancy analysis showed that soil N best explained leaf N and P variance,and tree biomass and C stock were related to biotic factors such as tree age and shrub biomass.Hierarchical partitioning analysis indicated that,compared with soil or litter variables,stand age only accounted for a relatively small proportion of leaf C,N,and P variation.Thus,secondary Quercus ecosystems might have inherent ability to maintain sensitive responses of metabolically active organs to environmental factors during stand aging.The results of this work help to elucidate the biogeochemical cycling of secondary forest ecosystems in tree development,provide novel insights into the adaptation strategies of plants in diff erent organs and growth stages,and could be used to guide fertilization programs and optimize forest structure.展开更多
[目的]探究渭北旱塬区不同年限撂荒地的土壤养分、胞外酶活性及其化学计量的变化特征及影响因素,以期为渭北旱塬区撂荒地的改善与管理提供一定的理论依据。[方法]以渭北旱塬不同年限(5 a, 10 a, 20 a, 25 a和33 a)的撂荒地为研究对象,...[目的]探究渭北旱塬区不同年限撂荒地的土壤养分、胞外酶活性及其化学计量的变化特征及影响因素,以期为渭北旱塬区撂荒地的改善与管理提供一定的理论依据。[方法]以渭北旱塬不同年限(5 a, 10 a, 20 a, 25 a和33 a)的撂荒地为研究对象,测定了土壤养分和参与土壤碳(C)、氮(N)和磷(P)循环的5种胞外酶活性,随后利用单因素方差分析、土壤胞外酶化学计量学模型和主坐标分析(PCoA)研究不同撂荒年限下土壤养分和胞外酶活性及其生态化学计量的变化规律及影响因子。[结果]随着撂荒年限的增加,土壤C和N获取酶活性显著减小,而P获取酶活性显著增加;土壤C、N和P含量变化与酶活性变化趋势相反。随撂荒年限延长,土壤微生物的C限制得到缓解,P限制逐渐加强。PCoA拟合环境因子分析结果显示:土壤可溶性有机碳(DOC)、总磷(TP)、速效氮(AN)和速效磷(AP)含量是驱动酶活性及其计量比变化的关键因子。[结论]撂荒对土壤养分状况具有显著改善作用,但随撂荒时间延长(20 a以上)会加剧微生物P限制,因此对经过长年撂荒的土地应当适量施用磷肥,以改善其土壤状况。展开更多
基金National Natural Science Foundation of China (52394195)Joint research program for ecological conservation and high-quality development of the Yellow River Basin (2022-YRUC-01-0304).
文摘The objectives of this study were to explore the changes in soil stoichiometry and enzyme activities at different distances from an opencast coal mine in the Hulun Buir Grassland of China. Four transects were established on north and east sides of the opencast coal mining area, and samples were collected at 50 m, 550 m, and 1550 m from the pit on each transect. Control samples were collected from a grassland station 8 km from the opencast coal mining area that was not disturbed by mining. Four replicate soil samples were collected at each point on the four transects. Soil physicochemical properties and enzyme activities were determined, and correlations between soil properties and stoichiometric ratios and enzyme activities were explored using redundancy analysis. The increase in distance from mining did not significantly affect soil properties, although soil urease activity was significantly lower than that of the control area. Soil properties 1550 m from the mine pit were similar to those at the grassland control. In addition, soil total nitrogen had the greatest effect on soil stoichiometry, and soil total potassium had the greatest effect on soil enzyme activities. Coal dust from opencast mining might be the main factor affecting soil stoichiometry and enzyme activities. The results of this study provide direction for the next step in studying the influence of mining areas on soil properties and processes.
基金supported by the National Natural Science Foundation of China(Nos.31800369,32271686,U1904204)the State Scholarship Fund of Chinathe Innovation Scientists and Technicians Troop Construction Projects of Henan Province(No.182101510005)。
文摘Background:Nitrogen(N)deposition affects forest stoichiometric flexibility through changing soil nutrient availability to influence plant uptake.However,the effect of N deposition on the flexibility of carbon(C),N,and phosphorus(P)in forest plant-soil-microbe systems remains unclear.Methods:We conducted a meta-analysis based on 751 pairs of observations to evaluate the responses of plant,soil and microbial biomass C,N and P nutrients and stoichiometry to N addition in different N intensity(050,50–100,>100 kg·ha^(-1)·year^(-1)of N),duration(0–5,>5 year),method(understory,canopy),and matter(ammonium N,nitrate N,organic N,mixed N).Results:N addition significantly increased plant N:P(leaf:14.98%,root:13.29%),plant C:P(leaf:6.8%,root:25.44%),soil N:P(13.94%),soil C:P(10.86%),microbial biomass N:P(23.58%),microbial biomass C:P(12.62%),but reduced plant C:N(leaf:6.49%,root:9.02%).Furthermore,plant C:N:P stoichiometry changed significantly under short-term N inputs,while soil and microorganisms changed drastically under high N addition.Canopy N addition primarily affected plant C:N:P stoichiometry through altering plant N content,while understory N inputs altered more by influencing soil C and P content.Organic N significantly influenced plant and soil C:N and C:P,while ammonia N changed plant N:P.Plant C:P and soil C:N were strongly correlated with mean annual precipitation(MAT),and the C:N:P stoichiometric flexibility in soil and plant under N addition connected with soil depth.Besides,N addition decoupled the correlations between soil microorganisms and the plant.Conclusions:N addition significantly increased the C:P and N:P in soil,plant,and microbial biomass,reducing plant C:N,and aggravated forest P limitations.Significantly,these impacts were contingent on climate types,soil layers,and N input forms.The findings enhance our comprehension of the plant-soil system nutrient cycling mechanisms in forest ecosystems and plant strategy responses to N deposition.
基金funded by the National Nature Science Foundation of China (No.41771556)funded by the Spanish Government Grants PID2020-115770RB-I00 and TED2021-132627B–I00+2 种基金funded by the Spanish MCIN,AEI/10.13039/501100011033the European Union NextGenerationEU/PRTR,the Catalan government grant SGR2021-1333the Fundaci on Ram on Areces grant CIVP20A6621
文摘We examined how afforestation patterns impact carbon(C),nitrogen(N),and phosphorus(P)stoichiometry in the plant-litter-soil system.Plant leaf,branch,stem,and root,litter,and soil samples were collected from mixedspecies plantations of Robinia pseudoacacia with Amygdalus davidiana(RPAD),R.pseudoacacia with Armeniaca sibirica(RPAS),and monocultures of R.pseudoacacia(RP),A.davidiana(AD),and A.sibirica(AS)in the Loess Hilly Region.The results showed that in mixed-species plantations,R.pseudoacacia had lower leaf N and P concentrations than in monocultures,while both A.davidiana and A.sibirica had higher leaf N and P concentrations.Soil P limited tree growth in both afforestation models.Mixing R.pseudoacacia with A.davidiana or A.sibirica reduced N-limitation during litter decomposition.Average soil total N and P concentrations were higher in RPAS than in RPAD,and both were higher than the corresponding monocultures.The average soil C:N ratio was the smallest in RPAS,while the average soil C:P ratio was larger in RPAS than in RP.A positive correlation between N and P concentrations,and between C:N and C:P ratios,was found in litter and all plant organs of mono-and mixedstands.Alternatively,for N concentration and C:N ratio,the correlations between plant(i.e.,leaf,branch,root)and litter and between plant and soil were inverse between plantation types.RPAD has an increased litter decomposition rate to release N and P,while RPAS has a faster rate of soil N mineralization.RPAD was the best plantation(mixed)to improve biogeochemical cycling,as soil nutrient restrictions,particularly for P-limitation,on trees growth were alleviated.This study thus provides insights into suitable tree selection and management by revealing C:N:P stoichiometry in the plant-litter-soil system under different afforestation patterns.
文摘Soybean and lentil are important legume crops in southern Saskatchewan (SK) that can supply the majority of their nitrogen (N) requirement through biological N fixation (BNF). However, the onset of BNF can be slow in cold;dry prairie soils and a small amount of seed-row placed fertilizer containing both N and phosphorus (P) may benefit the crop. Nevertheless, high rates of fertilizer in close proximity to the seed can also cause injury. This study was conducted to determine the response of lentil and soybean to a starter N-P fertilizer blend applied in the seed-row. A farm field located at the boundary of the Brown and Dark Brown soil zones in south-central Saskatchewan was selected to evaluate the effect of seed-row placed N-P fertilizer blend: 50% Urea + 50% mono-ammonium phosphate (MAP) applied at 0, 10, 20 and 30 kg N and P2O5 ha-1 on emergence, yield, and nutrient uptake. The proportion of nitrogen derived from fixation (ndff) was determined in the soybean using N-15 dilution technique. The rate of 10 kg N and P2O5 ha-1 was found to be the rate that did not significantly reduce emergence, stand count or proportion of N derived from fixation, and was sufficient to maximize yield, N and P uptake for both soybean and lentil under field conditions. Rates higher than 10 kg N ha-1 in the seed row as starter 28-26-0 blend reduced emergence and decreased the proportion of ndff.
基金supported by the Science and Technology Innovation Program of the Shaanxi Academy of Forestry(SXLK2022-02-03)the National Natural Science Foundation of China(42077452).
文摘Homeostasis is the adaptability of a species to a changing environment.However,the ecological stoichiometric homeostasis of Robinia pseudoacacia L.in diff erent climatic regions is poorly understood but could provide insights into its adaptability in the loess hilly region.This study sampled 20 year-old R.pseudoacacia plantations at 10 sites along a north–south transect on the Loess Plateau.Variations in the ecological stoichiometric characteristics of leaf and soil carbon,nitrogen,and phosphorus were analysed and homeostatic characteristics of leaf ecological stoichiometric parameters in diff erent climates were identifi ed.Factors aff ecting leaf stoichiometry were assessed.The results show that R.pseudoacacia leaves were rich in nitrogen and defi cient in phosphorous during tree growth and development.Nitrogen and phosphorous levels in the soils of the loess region were lower than the average in soils in the rest of China.All ecological stoichiometric parameters of R.pseudoacacia leaves in two diff erent climates were considered“strictly homeostasis”.Precipitation,available phosphorus,and soil C:P were the main factors aff ecting the variation of C:N:P stoichiometry of R.pseudoacacia leaves.R.pseudoacacia in the loess hilly region has strong ecologically homeostatic characteristics and suggests that it is well-adapted to the area.
基金supported by the National Nature Science Foundation of China(No.41977418 and 42130717)National Key R&D Program of China(2016YFC0501703 and 2017YFC0504605)CAS“Light of West China”Program(XAB201702).
文摘Ecological stoichiometry is an important indicator of biogeochemical cycles and nutrient limitations in terrestrial ecosystems.However,little is known about the response of ecological stoichiometry to plant growth.In this study,carbon(C),nitrogen(N),and phosphorus(P)concentrations were evaluated in plant tissues(trees,shrubs,and herbs),litter,and soil of young(≤40-year-old),middle-aged(41–60-year-old),near-mature(61–80-year-old),and mature(81–120-year-old)Quercus secondary forests on the Loess Plateau,China.Vegetation composition,plant biomass,and C stock were determined to illustrate their interaction with stoichiometry.Only tree biomass C signifi cantly increased with stand development.Leaf N and trunk P concentrationsgenerally increased,but branch P decreased with growth stage.Fine roots had the highest C and P concentrations at the middle-aged stage.In contrast,shrubs,herbs,litter,and soil C:N:P stoichiometry did not change signifi cantly during stand development.Leaf N and P were positively correlated with soil C,N,P,and their ratios.However,there was no signifi cant correlation between litter and leaves in terms of C:N:P stoichiometry.A redundancy analysis showed that soil N best explained leaf N and P variance,and tree biomass and C stock were related to biotic factors such as tree age and shrub biomass.Hierarchical partitioning analysis indicated that,compared with soil or litter variables,stand age only accounted for a relatively small proportion of leaf C,N,and P variation.Thus,secondary Quercus ecosystems might have inherent ability to maintain sensitive responses of metabolically active organs to environmental factors during stand aging.The results of this work help to elucidate the biogeochemical cycling of secondary forest ecosystems in tree development,provide novel insights into the adaptation strategies of plants in diff erent organs and growth stages,and could be used to guide fertilization programs and optimize forest structure.
文摘[目的]探究渭北旱塬区不同年限撂荒地的土壤养分、胞外酶活性及其化学计量的变化特征及影响因素,以期为渭北旱塬区撂荒地的改善与管理提供一定的理论依据。[方法]以渭北旱塬不同年限(5 a, 10 a, 20 a, 25 a和33 a)的撂荒地为研究对象,测定了土壤养分和参与土壤碳(C)、氮(N)和磷(P)循环的5种胞外酶活性,随后利用单因素方差分析、土壤胞外酶化学计量学模型和主坐标分析(PCoA)研究不同撂荒年限下土壤养分和胞外酶活性及其生态化学计量的变化规律及影响因子。[结果]随着撂荒年限的增加,土壤C和N获取酶活性显著减小,而P获取酶活性显著增加;土壤C、N和P含量变化与酶活性变化趋势相反。随撂荒年限延长,土壤微生物的C限制得到缓解,P限制逐渐加强。PCoA拟合环境因子分析结果显示:土壤可溶性有机碳(DOC)、总磷(TP)、速效氮(AN)和速效磷(AP)含量是驱动酶活性及其计量比变化的关键因子。[结论]撂荒对土壤养分状况具有显著改善作用,但随撂荒时间延长(20 a以上)会加剧微生物P限制,因此对经过长年撂荒的土地应当适量施用磷肥,以改善其土壤状况。
