Lyme disease(LD),caused by Borrelia burgdorferi,is themost common vector-borne disease in the United States and Europe.Despite the standard 2–4 weeks'antibiotic treatment,approximately 10%–20%of patients will de...Lyme disease(LD),caused by Borrelia burgdorferi,is themost common vector-borne disease in the United States and Europe.Despite the standard 2–4 weeks'antibiotic treatment,approximately 10%–20%of patients will develop posttreatment LD syndrome,a condition that is poorly understood.One of the probable causes is thought to be the presence of B.burgdorferi persister forms that are not effectively killed by the current LD antibiotics.In this study,we evaluated nitroxoline,an antibiotic used to treat urinary tract infections,for its activity against a stationary-phase culture enriched with persister forms of B.burgdorferi.Nitroxoline was found to be more active than doxycycline and equally active as cefuroxime(standard LD antibiotics)against B.burgdorferi.Importantly,the nitroxoline two-drug combinations nitroxoline+cefuroxime and nitroxoline+clarithromycin,as well as the nitroxoline three-drug combination nitroxoline+cefuroxime+clarithromycin,were as effective as the persister drug daptomycin-based positive control three-drug combination cefuroxime+doxycycline+daptomycin,completely eradicating stationary-phase B.burgdorferi in the drug-exposure experiments and preventing regrowth in the subculture study.Future studies should evaluate these promising drug combinations in a persistent LD mouse model.展开更多
基金supported by the Global Lyme Alliance,the Steven and Alexandra Cohen Foundation,the LivLyme Foundation,NatCapLyme,and the Einstein-Sim Family Charitable Fund.
文摘Lyme disease(LD),caused by Borrelia burgdorferi,is themost common vector-borne disease in the United States and Europe.Despite the standard 2–4 weeks'antibiotic treatment,approximately 10%–20%of patients will develop posttreatment LD syndrome,a condition that is poorly understood.One of the probable causes is thought to be the presence of B.burgdorferi persister forms that are not effectively killed by the current LD antibiotics.In this study,we evaluated nitroxoline,an antibiotic used to treat urinary tract infections,for its activity against a stationary-phase culture enriched with persister forms of B.burgdorferi.Nitroxoline was found to be more active than doxycycline and equally active as cefuroxime(standard LD antibiotics)against B.burgdorferi.Importantly,the nitroxoline two-drug combinations nitroxoline+cefuroxime and nitroxoline+clarithromycin,as well as the nitroxoline three-drug combination nitroxoline+cefuroxime+clarithromycin,were as effective as the persister drug daptomycin-based positive control three-drug combination cefuroxime+doxycycline+daptomycin,completely eradicating stationary-phase B.burgdorferi in the drug-exposure experiments and preventing regrowth in the subculture study.Future studies should evaluate these promising drug combinations in a persistent LD mouse model.
