The crystal structure of the title compound was determined by X-ray diffraction method at room temperature.The crystal belongs to triclinic space group P1 with the following crystallographic parameters:a=13.942(2),b=1...The crystal structure of the title compound was determined by X-ray diffraction method at room temperature.The crystal belongs to triclinic space group P1 with the following crystallographic parameters:a=13.942(2),b=15.540(2),c=10.007(1) A,a=105.16(1)°,β=92.42(1)°,γ=88.12(1)°,V=2090.2(4) Z=4,Dc=1.23g cm-3,F(000)=820,μ(MoKα)=0.74 cm-1,and final R= 0.054 for 3208 observations.Two molecules , which are slightly different in conformation,coexist in an asymmetric unit. Some intermolecular distances are fairly short.The packing arrangement of the molecules in the crystal is also discussed.展开更多
Two series of novel spin-labeled derivatives of Cinobufagin（compounds 5 and 8a--8f in series 1 with five-membered ring nitroxyl free radical and compounds 6 and 9a-9f in series 2 with six-membered ring nitroxyl free ...Two series of novel spin-labeled derivatives of Cinobufagin（compounds 5 and 8a--8f in series 1 with five-membered ring nitroxyl free radical and compounds 6 and 9a-9f in series 2 with six-membered ring nitroxyl free radical） were synthesized. The cytotoxic activities in vitro against two tumor cell lines（HepG2 and HeLa） were evaluated, and the results indicate that all compounds display potent cytotoxicity against HepG2 and HeLa cells, and most compounds show better activities on HeLa cells than on HepG2 cells except for compounds 8a and 9d. Gene- rally, the compounds in series 2 have more potent cytotoxic activity against HepG2 than the compounds in series 1. Especially, compounds 6 and 9f in series 2 exhibit even more potent activities against the two tumor cell lines than Cinobufagin. Thus incorPoration of different L-amino acids as the linker changed the cytotoxic profile of the spin-labeled Cinobufagin. In addition, the representative compound 9f significantly changed the cell cycle distribution and led to HeLa cell cycle arrested at G2/M phase.展开更多
文摘The crystal structure of the title compound was determined by X-ray diffraction method at room temperature.The crystal belongs to triclinic space group P1 with the following crystallographic parameters:a=13.942(2),b=15.540(2),c=10.007(1) A,a=105.16(1)°,β=92.42(1)°,γ=88.12(1)°,V=2090.2(4) Z=4,Dc=1.23g cm-3,F(000)=820,μ(MoKα)=0.74 cm-1,and final R= 0.054 for 3208 observations.Two molecules , which are slightly different in conformation,coexist in an asymmetric unit. Some intermolecular distances are fairly short.The packing arrangement of the molecules in the crystal is also discussed.
基金Supported by the National Natural Science Foundation of China（No. 81573315）, the Natural Science Foundation of Guangdong Province, China（No.2015A030313313）, the Guangzhou Industry-University Collaborative Innovation Major Projects, China（No.201508030016） and the Natural Science Foundation of Hainan Province, China（No.817307）.
文摘Two series of novel spin-labeled derivatives of Cinobufagin（compounds 5 and 8a--8f in series 1 with five-membered ring nitroxyl free radical and compounds 6 and 9a-9f in series 2 with six-membered ring nitroxyl free radical） were synthesized. The cytotoxic activities in vitro against two tumor cell lines（HepG2 and HeLa） were evaluated, and the results indicate that all compounds display potent cytotoxicity against HepG2 and HeLa cells, and most compounds show better activities on HeLa cells than on HepG2 cells except for compounds 8a and 9d. Gene- rally, the compounds in series 2 have more potent cytotoxic activity against HepG2 than the compounds in series 1. Especially, compounds 6 and 9f in series 2 exhibit even more potent activities against the two tumor cell lines than Cinobufagin. Thus incorPoration of different L-amino acids as the linker changed the cytotoxic profile of the spin-labeled Cinobufagin. In addition, the representative compound 9f significantly changed the cell cycle distribution and led to HeLa cell cycle arrested at G2/M phase.
