The otolith morphology of two croaker species(C ollichthys lucidus and C ollichthys niveatus) from three areas(Liaodong Bay, LD; Huanghe(Yellow) River estuary, HRE; Jiaozhou Bay, JZ) along the northern Chinese coast w...The otolith morphology of two croaker species(C ollichthys lucidus and C ollichthys niveatus) from three areas(Liaodong Bay, LD; Huanghe(Yellow) River estuary, HRE; Jiaozhou Bay, JZ) along the northern Chinese coast were investigated for species identifi cation and stock discrimination. The otolith contour shape described by elliptic Fourier coefficients(EFC) were analysed using principal components analysis(PCA) and stepwise canonical discriminant analysis(CDA) to identify species and stocks. The two species were well dif ferentiated, with an overall classifi cation success rate of 97.8%. And variations in the otolith shapes were significant enough to discriminate among the three geographical samples of C. lucidus(67.7%) or C. niveatus(65.2%). Relatively high mis-assignment occurred between the geographically adjacent LD and HRE samples, which implied that individual mixing may exist between the two samples. This study yielded information complementary to that derived from genetic studies and provided information for assessing the stock structure of C. lucidus and C. niveatus in the Bohai Sea and the Yellow Sea.展开更多
基金Supported by the National Key Basic Research Program of China(973 Program)(No.2015CB453302)the NSFC-Shandong Joint Fund for Marine Science Research Centre(No.U1606404)the Aoshan Science and Technology Innovation Project(No.2015ASKJ02-04)
文摘The otolith morphology of two croaker species(C ollichthys lucidus and C ollichthys niveatus) from three areas(Liaodong Bay, LD; Huanghe(Yellow) River estuary, HRE; Jiaozhou Bay, JZ) along the northern Chinese coast were investigated for species identifi cation and stock discrimination. The otolith contour shape described by elliptic Fourier coefficients(EFC) were analysed using principal components analysis(PCA) and stepwise canonical discriminant analysis(CDA) to identify species and stocks. The two species were well dif ferentiated, with an overall classifi cation success rate of 97.8%. And variations in the otolith shapes were significant enough to discriminate among the three geographical samples of C. lucidus(67.7%) or C. niveatus(65.2%). Relatively high mis-assignment occurred between the geographically adjacent LD and HRE samples, which implied that individual mixing may exist between the two samples. This study yielded information complementary to that derived from genetic studies and provided information for assessing the stock structure of C. lucidus and C. niveatus in the Bohai Sea and the Yellow Sea.