Effects of luteinizing hormone and androgen on the development of rat progenitor Leydig cells in vitro and in vivo

Progenitor Leydig cells are derived from stem cells. The proliferation and differentiation of progenitor Leydig cells significantly contributes to Leydig cell number during puberty. However, the regulation of these processes remains unclear. The objective of the present study was to determine whether luteinizing hormone （LH） or androgen contributes to the proliferation and differentiation of progenitor Leydig cells. Fourteen-day-old male Sprague-Dawley rats were treated for 7 days with NalGlu, which is a gonadotropin- releasing hormone antagonist, to reduce the secretion of LH in the pituitary and thus, androgen in the testis. Rats were co-administered with LH or 7a-methyl-nortestosterone （MENT）, which is an androgen resistant to metabolism by 5a-reductase 1 in progenitor Leydig cells, and the subsequent effects of LH or androgen were measured. 3H-Thymidine was also intravenously injected into rats to study thymidine incorporation in progenitor Leydig cells. Progenitor Leydig cells were examined. NalGlu administration reduced progenitor Leydig cell proliferation by 83%. In addition, LH or MENT treatment restored Leydig cell proliferative capacity to 73% or 50% of control, respectively. The messenger RNA levels of proliferation-related genes were measured using real-time PCR. The expression levels of Igfl, Lifr, Pdgfra, Bcl2, Ccnd3and Pcnawere upregulated by MENT, and those of Pdgfra, Ccnd3and Pcnawere upregulated by LH. Both LH and MENT stimulated the differentiation of progenitor Leydig cells in vitro. We concluded that both LH and MENT were involved in regulating the development of progenitor Leydig cells.

复方木尼孜其颗粒联合四环素治疗寻常型痤疮的临床疗效观察

目的观察复方木尼孜其颗粒联合四环素治疗寻常型痤疮的临床疗效。方法 85例患者随机分为治疗组43例和对照组42例,对照组仅给予四环素治疗,治疗组在对照组治疗基础上加用复方木尼孜其颗粒。2组疗程均为6周。观察比较2组治疗后第2、4、6周临床疗效和不良反应。结果治疗组治疗后第2、4、6周总有效率分别为88.37%、90.70%、95.35%,高于对照组的69.05%、71.43%、78.57%,差异均有统计学意义(P<0.05)。2组均未见严重不良反应。结论复方木尼孜其颗粒联合四环素治疗寻常型痤疮疗效满意,无明显不良反应,值得临床推广使用。

水滑石/硫酸镁复合体系对内墙腻子保温性能的影响

通过添加具有红外阻隔能力的填料,将建筑物内部发射的红外线反射回去,降低能量的损失,从而达到节能的效果。本文使用傅立叶变换红外光谱仪,导热系数仪和智能数字式巡回检测温度测试仪分别测试了添加具有红外阻隔能力填料的腻子的红外吸收光谱,导热系数和温度变化情况,通过测试结果得出结论:水滑石和硫酸镁在4:8的时候,红外阻隔性能最优,其温差变化较没有添加水滑石硫酸镁复合体系时提高5℃左右,而对腻子的导热系数几乎没有影响,这是由于填料对腻子的保温机理不同所致,同时也进一步验证了填料是通过红外阻隔来达到保温效果的。

论《性自命出》与公孙尼子的关系

通过对《性自命出》与公孙尼子思想的细致比较研究，可以表明《礼记·乐记》的心性论内涵是颇为深刻、丰富的。《乐记》中有关心性的论述，尤其是有关道与欲、天理与人欲的理解，以及以“正义”论身心关系，都比《性自命出》更深入、更高阔。结合其它有关世传文献的考察，似难推定《性自命出》为公孙尼子所作。

试论《乐记》的作者及与子思学派的关系

有关今本《乐记》的作者问题一直是有争议的,从传统文献与出土文献两个角度进行考察,《乐记》最有可能的作者是战国时的公孙尼子。通过《乐记》与《性自命出》、《荀子.乐论》的对比研究,进一步肯定了《乐记》作于公孙尼子之说。公孙尼子的思想与子思学派接近,或说公孙尼子原本就属于子思一系。因此,研究子思学派可以把《乐记》纳入考察之范围。

对于《乐记》的成书年代与作者的质疑

先秦音乐思想以及整个文艺思想 ,集中表现在一场关于“乐”的论争上。作为这场论争的总结和结晶的《乐记》 ,其成书年代历来没有弄清楚。郭沫若曾提出了公孙尼子说。但从《乐记》自身的矛盾性来看 ,作者不应是公孙尼子 ;从儒家传统文艺观的发展过程来看 ,《乐记》当在《荀子》之后。从直接的史料记载看《乐记》的成书年代与作者 ,《汉书·艺文志》提供的史料是基本可信的。所以 ,《乐记》应是汉武帝时 ,刘德与毛生等人收集《周官》及诸子言乐事者所作 ,经汉成帝时刘向校书而得。