Using AFM,we observed linear chain-like complexes formed by some specific proteins and the multi-mRNAs during the in vitro expression of some active genes on the DNA fragments. The LDH mRNA in the multi-mRNA complex c...Using AFM,we observed linear chain-like complexes formed by some specific proteins and the multi-mRNAs during the in vitro expression of some active genes on the DNA fragments. The LDH mRNA in the multi-mRNA complex can in vitro translate LDH. Via AFM, we also discovered that nmRNA prepared from heart muscles, along with some specific proteins can form linear chain-like nmRNA complexes in which LDH mRNA can also translate LDH in vitro. Our work shows the prospective application of AFM in the research of the biological reaction of the active genes on the DNA fragments.展开更多
It is observed that the regulation sequences at the two ends of every active gene of the heart nuclear DNA fragments may differentially combine with active regulation factors such as some specific binding proteins by ...It is observed that the regulation sequences at the two ends of every active gene of the heart nuclear DNA fragments may differentially combine with active regulation factors such as some specific binding proteins by using AFM and other experimental technologies. These active genes form different "gene knots", which are separated by "intervals". Using AFM, occasionally, it is also discovered that during the transcription stage, the heart nuclear DNA fragments consist of 3 4 5 "gene knots" and related "intervals", which form various "gene lineages" respectively by some "permutation and combination". Each gene lineage is likely to form nRNA chain like complexes that are 3 times the quantity of gene knots, and each nRNA chain like complex is connected with both ends of corresponding gene lineage. One gene knot of the DNA fragments participates the formation of different gene lineage and corresponding RNA chain like complexes by different combination. By posttranscriptional modification, they can form nmRNA linear chain like complexes that show the speciality of tissues. The beginnings of transcription units have the same number as gene lineages, and all gene lineages in DNA molecules may transcribe efficiently from corresponding beginnings of transcription unit simultaneously. Our work shows the prospective application of AFM in the research of the diversity of gene lineages formation from gene knots in the transcription stage and the efficiency of gene knots transcription.展开更多
文摘Using AFM,we observed linear chain-like complexes formed by some specific proteins and the multi-mRNAs during the in vitro expression of some active genes on the DNA fragments. The LDH mRNA in the multi-mRNA complex can in vitro translate LDH. Via AFM, we also discovered that nmRNA prepared from heart muscles, along with some specific proteins can form linear chain-like nmRNA complexes in which LDH mRNA can also translate LDH in vitro. Our work shows the prospective application of AFM in the research of the biological reaction of the active genes on the DNA fragments.
文摘It is observed that the regulation sequences at the two ends of every active gene of the heart nuclear DNA fragments may differentially combine with active regulation factors such as some specific binding proteins by using AFM and other experimental technologies. These active genes form different "gene knots", which are separated by "intervals". Using AFM, occasionally, it is also discovered that during the transcription stage, the heart nuclear DNA fragments consist of 3 4 5 "gene knots" and related "intervals", which form various "gene lineages" respectively by some "permutation and combination". Each gene lineage is likely to form nRNA chain like complexes that are 3 times the quantity of gene knots, and each nRNA chain like complex is connected with both ends of corresponding gene lineage. One gene knot of the DNA fragments participates the formation of different gene lineage and corresponding RNA chain like complexes by different combination. By posttranscriptional modification, they can form nmRNA linear chain like complexes that show the speciality of tissues. The beginnings of transcription units have the same number as gene lineages, and all gene lineages in DNA molecules may transcribe efficiently from corresponding beginnings of transcription unit simultaneously. Our work shows the prospective application of AFM in the research of the diversity of gene lineages formation from gene knots in the transcription stage and the efficiency of gene knots transcription.
