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Altered profiles of nuclear matrix proteins during the differentiation of human gastric mucous adenocarcinoma MGc80-3 cells 被引量:13
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作者 Chun-Hong Zhao Qi-Fu Li 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第30期4628-4633,共6页
AIM: To find and identify specific nuclear matrix proteins associated with proliferation and differentiation of carcinoma cells, which will be potential markers for cancer diagnosis and targets in cancer therapy. MET... AIM: To find and identify specific nuclear matrix proteins associated with proliferation and differentiation of carcinoma cells, which will be potential markers for cancer diagnosis and targets in cancer therapy. METHODS: Nuclear matrix proteins were selectively extracted from MGcS0-3 cells treated with or without hexamethylamine bisacetamide (HMBA), and subjected to 2-D gel electrophoresis. The resulted protein patterns were analyzed by Melanie software. Spots of nuclear matrix proteins differentially expressed were excised and subjected to in situ digestion with trypsin. Peptide masses were obtained by matrix-assisted laser-desorption/ ionization time of flight mass spectrometry (MALDI-TOFMS) analysis and submitted for database searching using Mascot tool. RESULTS: The MGc80-3 cells were induced into differentiation by HMBA. There were 22 protein spots which changed remarkably in the nuclear matrix, from differentiation of MGcS0-3 cells compared to control. Eleven of which were identified. Seven proteinsactin, prohibitin, porin 31HL, heterogeneous nuclear dbonucleoprotein A2/B1, vimentin, ATP synthase, and heat shock protein 60 were downregulated, whereas three proteins - heat shock protein gp96, heat shock protein 90-beta, and valosin-containing protein were upregulated, and the oxygen-regulated protein was only found in the differentiated MGc80-3 cells. CONCLUSION: The induced differentiation of carcinoma cells is accompanied by the changes of nuclear matrix proteins. Further characterization of those proteins will show the mechanism of cellular proliferation and differentiation, as well as cancer differentiation. 展开更多
关键词 Nuclear matrix proteins Cell differentiation human gastric mucous adenocarcinoma MGc80-3 Hexamethylamine bisacetamide
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Internal structures and high-velocity frictional properties of Longmenshan fault zone at Shenxigou activated during the 2008 Wenchuan earthquake 被引量:3
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作者 Yu Wang Shengli Ma +7 位作者 Toshihiko Shimamoto Lu Yao Jianye Chen Xiaosong Yang Honglin He Jiaxiang Dang Linfeng Hou Tetsuhiro Togo 《Earthquake Science》 2014年第5期499-528,共30页
This paper reports internal structures of a wide fault zone at Shenxigou,Dujiangyan,Sichuan province,China,and high-velocity frictional properties of the fault gouge collected near the coseismic slip zone during the 2... This paper reports internal structures of a wide fault zone at Shenxigou,Dujiangyan,Sichuan province,China,and high-velocity frictional properties of the fault gouge collected near the coseismic slip zone during the 2008 Wenchuan earthquake.Vertical offset and horizontal displacement at the trench site were 2.8 m(NW side up)and 4.8 m(right-lateral),respectively.The fault zone formed in Triassic sandstone,siltstone,and shale about 500 m away from the Yingxiu-Beichuan fault,a major fault in the Longmenshan fault system.A trench survey across the coseismic fault,and observations of outcrops and drill cores down to a depth of 57 m revealed that the fault zone consists of fault gouge and fault breccia of about0.5 and 250-300 m in widths,respectively,and that the fault strikes N62°E and dips 68° to NW.Quaternary conglomerates were recovered beneath the fault in the drilling,so that the fault moved at least 55 m along the coseismic slip zone,experiencing about 18 events of similar sizes.The fault core is composed of grayish gouge(GG) and blackish gouge(BG) with very complex slip-zone structures.BG contains low-crystalline graphite of about 30 %.High-velocity friction experiments were conducted at normal stresses of 0.6-2.1 MPa and slip rates of 0.1-2.1 m/s.Both GG and BG exhibit dramatic slip weakening at constant high slip rates that can be described as an exponential decay from peak friction coefficient lpto steadystate friction coefficient lssover a slip-weakening distance Dc.Deformation of GG and BG is characterized by overlapped slip-zone structures and development of sharp slickenside surfaces,respectively.Comparison of our data with those reported for other outcrops indicates that the high-velocity frictional properties of the Longmenshan fault zones are quite uniform and the high-velocity weakening must have promoted dynamic rupture propagation during the Wenchuan earthquake. 展开更多
关键词 Wenchuan earthquake - Longmenshan faultsystem - Shenxigou fault zone Fault zone structures High-velocity friction
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Neuroprotective effects of human telomerase reverse transcriptase on beta-amyloid fragment 25-35-treated human embryonic cortical neurons 被引量:3
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作者 Lingping Kong Lingzhi Wu +2 位作者 Jie Zhang Yaping Liao Huaqiao Wang 《Neural Regeneration Research》 SCIE CAS CSCD 2009年第6期405-412,共8页
BACKGROUND: Numerous current studies have suggested that human telomerase reverse transcriptase (hTERT) gene has neuroprotective effects and can inhibit apoptosis induced by various cytotoxic stresses; however, the... BACKGROUND: Numerous current studies have suggested that human telomerase reverse transcriptase (hTERT) gene has neuroprotective effects and can inhibit apoptosis induced by various cytotoxic stresses; however, the mechanism of action remains unknown. OBJECTIVE: To evaluate the neuroprotective effects and possible mechanism of action of hTERT gene transfection in human embryonic cortical neurons treated with beta-amyloid fragment 25-35 (AI325-35). DESIGN, TIME AND SETTING: The randomized, controlled and molecular biological studies were performed at the Department of Anatomy and Brain Research, Zhongshan School of Medicine, Sun Yat-sen University, China, from September 2005 to June 2008. MATERIALS: AdEasy-1 Expression System was gifted by Professor Guoquan Gao from Sun Yat-Sen University, China. Human cortical neurons were derived from 12-20 week old aborted fetuses, obtained from the Guangzhou Maternal and Child Health Hospital, China. Mouse anti-Odk5 and mouse anti-p16 monoclonal antibodies (Lab Vision, USA), and mouse anti-hTERT monoclonal antibody (Epitomics, USA), were used in this study. METHODS: (1) Recombinant adenovirus vectors, encoding hTERT (Ad-hTERT) and green fluorescent protein (Ad-GFP), were constructed using the AdEasy-1 Expression System. Human embryonic cortical neurons in the Ad-hTERT group were transfected with Ad-hTERT for 1-21 days. Likewise, human embryonic cortical neurons in the Ad-GFP group were transfected with Ad-GFP for 1-21 days. Human embryonic cortical neurons in the control group were cultured as normal. (2) Human embryonic cortical neurons in the Ad-hTERT group were treated with 10 pmol/L Aβ25-35 for 24 hours. Normal human embryonic cortical neurons treated with 10 pmol/Lβ25.35 for 24 hours served as a model group. Human embryonic cortical neurons in the Ad-GFP and control groups were not treated with Aβ25-35. MAIN OUTCOME MEASURES: Expression of hTERT in human embryonic cortical neurons was evaluated by immunocytochemical staining and Western blot assay. Telomerase activity was measured using a PCR-based telomeric repeat amplification protocol (TRAP) ELISA kit. Neural activity in human embryonic cortical neurons was examined by MTT assay; apoptosis was measured using TUNEL assay; and Cdk5 and p16 protein expressions were measured by Western blot. RESULTS: Expression of hTERT protein was significantly increased and peaked at day 3 post-transfection in the Ad-hTERT group. No hTERT expression was detected in the Ad-GFP and control groups. Telomerase activity was significantly greater in the Ad-hTERT group compared with the Ad-GFP and control groups (P 〈 0.01). Compared with the control group, cell activity was significantly decreased (P 〈 0.05), and cell apoptotic rate, Cdk5 and p16 expression were significantly increased (P 〈 0.01) in the model group. Compared with the model group, cell activity was increased in the Ad-hTERT group, and peaked at day 3 post-transfection (P 〈 0.05). Neuroprotective effects also peaked at day 3 post-transfection; and the apoptotic rate, Cdk5 and p16 expression significantly decreased (P 〈 0.01). CONCLUSION: Expression of hTERT in human embryonic cortical neurons can relieve Aβ25-35-induced neuronal apoptosis. The possible mechanism by which hTERT produces these neuroprotective effects may be associated with inhibition of Cdk5 and p16 expression. 展开更多
关键词 human telomerase reverse transcriptase cortical neuron human embryo Alzheimer's disease beta-amyloid fragment 25-35 CDK5 P16
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Development of a sensitive and rapid method for quantitation of (S)-(-)- and (R)-(+)-metoprolol in human plasma by chiral LC–ESI–MS/MS 被引量:3
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作者 Primal Sharma Pritesh Contractor +2 位作者 Swati Guttikar Daxesh P.Patel Pranav S.Shrivastav 《Journal of Pharmaceutical Analysis》 SCIE CAS 2014年第1期63-79,共17页
A selective, sensitive and high throughput liquid chromatography-tandem mass spectro-metry (LC-ESI-MS/MS) method has been developed for separation and quantification of metoprolol enantiomers on a chiral Lux Amylose... A selective, sensitive and high throughput liquid chromatography-tandem mass spectro-metry (LC-ESI-MS/MS) method has been developed for separation and quantification of metoprolol enantiomers on a chiral Lux Amylose-2 (250 mm&#215;4.6 mm, 5 mm) column. Solid phase extraction of (S)-(-)- and (R)-(t)-metoprolol and rac-metoprolol-d6 as an internal standard (IS) was achieved on Lichrosep DVB HL cartridges employing 200 mL human plasma. Both the analytes were chromatographically separated with a resolution factor of 2.24 using 15 mM ammonium acetate in water, pH 5.0 and 0.1% (v/v) diethyl amine in acetonitrile (50:50, v/v) as the mobile phase within 7.0 min. The precursor-product ion transitions for the enantiomers and IS were monitored in the multiple reaction monitoring and positive ionization mode. The method was validated over the concentration range of 0.500-500 ng/mL for both the enantiomers. Matrix effect was assessed by post-column analyte infusion experiment and the mean extraction recovery was greater than 94.0% for both the enantiomers at all quality control levels. The stability of analytes was evaluated in plasma and whole blood under different storage conditions. The method was successfully applied to a clinical study in 14 healthy volunteers after oral administration of 200 mg metoprolol tablet under fasting conditions. The assay reproducibility is shown by reanalysis of 68 incurred samples. The suitability of the developed method was assessed in comparison with&amp;nbsp;different chromatographic methods developed for stereoselective analysis of metoprolol in biological matrices. 展开更多
关键词 S---metoprolol R-(+)-metoprolol Chiral column Chromatographicseparation LC-ESI-MS/MS human plasma
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数据要素赋能对人力资本升级的作用机制分析--基于国家级大数据综合试验区数据 被引量:2
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作者 张珂涵 张古 赵兴罗 《科技管理研究》 CSSCI 2024年第3期174-182,共9页
以国家级大数据综合试验区作为实证场域,基于2011—2021年沪深A股上市公司数据,采用基准回归分析平行趋势检验方法检验了数据要素赋能对于企业人力资本升级的促进作用并进行相关的稳健性与异质性分析,在此基础上,对数据赋能效果的创新... 以国家级大数据综合试验区作为实证场域,基于2011—2021年沪深A股上市公司数据,采用基准回归分析平行趋势检验方法检验了数据要素赋能对于企业人力资本升级的促进作用并进行相关的稳健性与异质性分析,在此基础上,对数据赋能效果的创新激励效应和规模扩张效应两者作用机制以及对企业低技能劳动力组别、雇佣规模以及人力资本相对结构的影响进行进一步探讨。研究结果表明,国家级大数据综合试验区建设的数据要素赋能功效有力推动了企业人力资本升级与人才队伍扩张,一系列的稳健性检验有力支撑了上述结论;企业层面产权性质与创新属性的不同、区域层面的地理区位与行政层级的区别导致了经营策略与资源禀赋差异,对企业人力资本升级产生了异质性影响;国家级大数据综合试验区建设畅通了企业数字化转型渠道,通过创新激励效应和规模扩张效应作用于人力资本升级进程;扩展性讨论表明,数据要素赋能也会增加部分低技能劳动力岗位,带来企业雇佣规模的扩张。因此,需要充分发挥数据要素的赋能功效,推动数据要素与企业生产经营过程的深度耦合,最终实现人力资本的优化升级,为培育壮大企业发展的新动能注入人才动力。 展开更多
关键词 数据要素 国家级大数据综合试验区 人力资本升级
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基于自然与人文耦合视域的高寒生态功能区宜居性评价及阻滞因子诊断--以青海省为例 被引量:1
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作者 殷悦 周侃 +1 位作者 湛东升 陈妤凡 《生态学报》 CAS CSCD 北大核心 2024年第3期992-1008,共17页
宜居性是自然生态环境和人文社会环境的有机统一体,厘清高寒生态功能区宜居性及其阻滞因子,是精准提升生态安全屏障内宜居水平并促进其人与自然和谐共生的重要前提。基于城乡宜居性评价基本范式、顾及高寒生态功能区人居系统的独特性,... 宜居性是自然生态环境和人文社会环境的有机统一体,厘清高寒生态功能区宜居性及其阻滞因子,是精准提升生态安全屏障内宜居水平并促进其人与自然和谐共生的重要前提。基于城乡宜居性评价基本范式、顾及高寒生态功能区人居系统的独特性,从自然与人文耦合视域构建由自然环境、居住环境、设施环境、社会环境4个维度、8个一级指标及24个二级指标组成的高寒生态功能区宜居性指标体系,以青海省45个县域单元为例定量评价其宜居性及阻滞因子,并探讨宜居性与生态重要性、社会经济发展状态的交互关系。结果显示:(1)青海省总体宜居水平偏低,并呈现由河湟谷地生态区、柴达木盆地生态区、环青海湖生态区、三江源生态区依次递减的地域分异;(2)宜居水平和生态重要性的空间耦合不协调,三江源生态区等生态重要区的宜居水平低,宜居水平与人口规模、经济发展均成正比;(3)青海省宜居性主要受到居住环境和社会环境的阻滞,生态地理分区层面,在自然因子的主要制约下,三江源生态区、环青海湖生态区、柴达木盆地生态区分别受设施、居住、社会环境的强约束;(4)针对不同生态地理分区宜居性特征提出未来人口引导及宜居性优化模式,即按宜居性及社会经济生态引力明确人口集聚型、疏散型和稳定型3种空间集疏类型,通过人口城镇化、就业移民、教育移民等跨区域移民及就近生态移民增强宜居性与人口分布的空间适配性,以三江源生态区等弱势区域以及饮水设施、道路设施、物流配送、网络通讯等基础设施阻滞因子为重点,提升高寒生态功能区人居系统的设施配置与供给能力。 展开更多
关键词 宜居性 阻滞因子 自然与人文耦合 生态地理分区 高寒生态功能区
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THE DIFFERENTIATION OF HUMAN GASTRIC ADENOCAR-CINOMA CELL LINE MGc80-3 INDUCED BY DIBUTYRYL cAMP IN VITRO 被引量:2
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作者 李祺福 汪德耀 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1991年第1期7-13,共7页
For providing some experimental basis in establishing malignant phenotypic reversed indexes of gastric carcinoma cells, human gastric adenocar-cinoma cell line MGc80-3 was induced by dBcAMP in vitro to appraise the ef... For providing some experimental basis in establishing malignant phenotypic reversed indexes of gastric carcinoma cells, human gastric adenocar-cinoma cell line MGc80-3 was induced by dBcAMP in vitro to appraise the effect of gastric carcinoma cell differentiation by chemical inducers.Under light microscope, MGc80-3 cells, after treated with 1 mM dBcAMP, tended to be flat and disperse, and their volume gradually enlarged, with their uncleus relatively smaller and their shape rather regular. Morphological changes, like norma differentiated epithelial cells, were observed. The cells attached firmly, grew slowly, their growth curve showed inhibitory rate amounted to 52.87%, and cellular division exponent displayed their peak value 1.5 times less than that of MGc80-3 cells. It was clear that dBcAMP could effectively inhibit the multiplication activity of MGc80-3 cells. After dBcAMP treatment, remarkable changes of cell surface charges was indicated by cell electrophoresis, the ratio dropped to 3.043 from 3.988, and their re-tardant ratio reached up to 31.2%. cAMP content in cells after this treatment, detected by cAMP and cGMP radioimmunoassay, was enhanced by 2.42 times, and cAMP/cGMP ratio, by 1.73 times. Thus, cAMP level within MGc80-3 cells was raised obviously by dBcAMP. Heterotransplantation experiments showed that tuntorigenic rate of MGc80-5 cells (transplanted subcutaneously to BALB/c mice) amounted to 100%, and that of the cells after this treatment was only 5.6%. Their tumorigenic ability was extremely reduced.These results confirmed that dBcAMP was able to change malignant phenotypic characteristics of MGc80-3 cells and produce a reversed alteration: Thus, it has a remarkable inductive effect in differentiating gastric carcinoma cells. All these characteristics were also considered as the reference indexes in appraising reversed effect for the homologous cancer cells. 展开更多
关键词 THE DIFFERENTIATION OF human GASTRIC ADENOCAR-CINOMA CELL LINE MGc80-3 INDUCED BY DIBUTYRYL cAMP IN VITRO
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circ_0003204 regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis 被引量:1
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作者 Liyuan Yu Kai Xia +5 位作者 Jing Zhou Zhiai Hu Xing Yin Chenchen Zhou Shujuan Zou Jun Liu 《International Journal of Oral Science》 SCIE CAS CSCD 2022年第3期360-370,共11页
Human adipose-derived stem cells(hASCs)are a promising cell type for bone tissue regeneration.Circular RNAs(circRNAs)have been shown to play a critical role in regulating various cell differentiation and involve in me... Human adipose-derived stem cells(hASCs)are a promising cell type for bone tissue regeneration.Circular RNAs(circRNAs)have been shown to play a critical role in regulating various cell differentiation and involve in mesenchymal stem cell osteogenesis.However,how circRNAs regulate hASCs in osteogenesis is still unclear.Herein,we found circ_0003204 was significantly downregulated during osteogenic differentiation of hASCs.Knockdown of circ_0003204 by si RNA or overexpression by lentivirus confirmed circ_0003204 could negatively regulate the osteogenic differentiation of hASCs.We performed dual-luciferase reporting assay and rescue experiments to verify circ_0003204 regulated osteogenic differentiation via sponging miR-370-3p.We predicted and confirmed that miR-370-3p had targets in the 3′-UTR of HDAC4 m RNA.The following rescue experiments indicated that circ_0003204 regulated the osteogenic differentiation of hASCs via miR-370-3p/HDAC4 axis.Subsequent in vivo experiments showed the silencing of circ_0003204 increased the bone formation and promoted the expression of osteogenic-related proteins in a mouse bone defect model,while overexpression of circ_0003204 inhibited bone defect repair.Our findings indicated that circ_0003204 might be a promising target to promote the efficacy of hASCs in repairing bone defects. 展开更多
关键词 regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis MIR
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Whether human-induced activities could change the gradient pattern of coastal land use along the sea-land direction:a case study in Manila Bay,Philippines
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作者 Zhi Ding Fenzhen Su +7 位作者 Yanan Chen Ying Liu Xue Feng Wenqiu Hu Fengqin Yan He Li Pujia Yu Xuguang Tang 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2023年第2期163-174,共12页
Increasing intense human activities have largely changed the coastal landscape and caused many environmental issues.However,whether human-induced activities could change the coastal land use gradient pattern,an import... Increasing intense human activities have largely changed the coastal landscape and caused many environmental issues.However,whether human-induced activities could change the coastal land use gradient pattern,an important coastal zonal characteristic along the sea-land direction,remains unclear.Manila Bay was selected as the study area in this work.According to the distance of the land use and land cover(LULC)to the coastline,we clustered the typical coastal land use sequence patterns(CLUSPs)along the sea-land direction between 1988 and 2016 in Manila Bay and found the following.(1)Four typical CLUSPs,including the natural CLUSP dominated by forest land and grassland,the agricultural CLUSP dominated by dry farm and paddy field,the urbanised CLUSP dominated by construction land and the fishery CLUSP dominated by fishing farm,were mined in 1988.Three typical CLUSPs(a natural CLUSP,an intermediate CLUSP between the agricultural and urbanised CLUSPs,and a fishery CLUSP)were mined in 2016.(2)Affected by the dominant LULC,these typical CLUSPs showed a regular spatial pattern along the sea-land direction.For example,the typical natural CLUSP showed a landward pattern due to the long distance between the forest land and grassland and the coastline.(3)However,influenced by urban and aquaculture expansion,the land intensification of the CLUSP exhibited an obvious increase and caused the decrease of the CLUSP diversity from 1988 to 2016.The increase in the area of LULC coverage showed no obvious correlation with its distance from the coastline(DFC),but the net increase rate of LULC coverage had a significant negative correlation with the DFC.Therefore,human-induced activities have a large impact on the gradient pattern of coastal land use along the sea-land direction. 展开更多
关键词 coastal zone land use gradient sea-land human activities Manila Bay
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Relationship between chromosome behaviour and stability of human-mouse and human-(human-mouse)hvbridomas
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作者 梁喆 王德堂 金伯泉 《Journal of Medical Colleges of PLA(China)》 CAS 1993年第2期114-119,共6页
Chromosomes in human-mouse and human-(human-mouse)hybridomas wereanalysed by G-banding methods.It was found that most human chromosomes,exceptNo.13 and X,Y,were retained.The frequencies of chromosomes No.1,3,4,5,6,17,... Chromosomes in human-mouse and human-(human-mouse)hybridomas wereanalysed by G-banding methods.It was found that most human chromosomes,exceptNo.13 and X,Y,were retained.The frequencies of chromosomes No.1,3,4,5,6,17,19,21 and 22 were higher than those of other chromosomes in each hybridoma clone.The myeloma cell lines X63-Ag8.653 and SHM-D33 were also analysed.The morphologyof marker chromosomes was apparently different between hybridomas.There were 7 kindsof marker chromosomes in human-mouse hybridomas and 16 kinds of markerchromosomes in human-(human-mouse)hybridomas.Clones that retained humanchromosome No.1 were more stable and clones that did not retain human chromosomeNo.14 were still capable of secreting human immunoglobulin.Clones that retained humanchromosome No.2 did not secret human k light chain McAb while clones that retainedhuman chromosomes No.2 and No.22 only secreted λ light chain. 展开更多
关键词 HYBRIDOMAS IMMUNOGLOBULINS GIEMSA STAINING chromosomes human 1-3 chromosomes human 13-15 chomosomes human 21-22
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Auto-measuring System of 3-Dimensional Human Body
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作者 李勇 尚保平 +1 位作者 付小莉 尚会超 《Journal of Donghua University(English Edition)》 EI CAS 2001年第3期110-112,共3页
To realize the automation of fashion industry measuring,designing and manufacturing, the auto-measurement of 3D size of human body is of great importance. The auto measurement system of 3D human body based on Charge C... To realize the automation of fashion industry measuring,designing and manufacturing, the auto-measurement of 3D size of human body is of great importance. The auto measurement system of 3D human body based on Charge Coupled Devices (CCD) and infrared sensors is presented in this paper. The system can measure the bare size of human body that excludes the effect of clothing quickly and accurately. 展开更多
关键词 3- D measurement human BODY measurement.
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Factors Influencing the Successful Adoption of Human Resource Information System: The Content of Aqaba Special Economic Zone Authority
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作者 Hisham Al-Mobaideen Sattam Allahawiah Eman Basioni 《Intelligent Information Management》 2013年第1期1-9,共9页
This study examines the key factors that have impact on the successful adoption of Human Resource Information System (HRIS) within the Aqaba Special Economic Zone Authority (ASEZA)/Jordan. In order to accomplish the p... This study examines the key factors that have impact on the successful adoption of Human Resource Information System (HRIS) within the Aqaba Special Economic Zone Authority (ASEZA)/Jordan. In order to accomplish the purpose of the study four critical factors are inquired. So, four critical factors are inquired: First, TAM Model (Perceived Ease of Use (PEOU) and Perceived Usefulness (PU)). Second, Information Technology Infrastructure (ITI). Third, Top Management Support (TMS). Finally, Individual Experience with Computer (IEC). The research model was applied to collect data from the questionnaires answered by 45 users of HRIS as a source of primary data, based on a convenience sample the response rate was about 91%. In addition, the results were analyzed by utilizing the Statistical Package for Social Software (SPSS). Furthermore, the findings were analyzed;multiple Regression analysis indicated that all research variables have significant relationship on successful adoption of HRIS. The findings indicated IT infrastructures have a positive and significant effect on the successful adoption of HRIS. But there is no significant of PU, PEOU, TMS, and IEC on the successful adoption of HRIS. Finally, the results indicated that no significant statistical differences of demographic characteristics on HRIS adoption. Depending on the research’s findings;the researchers proposed a set of recommendations for better adoption of HRIS in SEZA. 展开更多
关键词 human Resource INFORMATION System (HRIS) Aqaba Special Economic zone AUTHORITY (ASEZA) PERCEIVED USEFULNESS (PU) INFORMATION Technology Infrastructure (ITI) Top Management Support (TMS) and Individual Experience with Computer (IEC)
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CHEMOSENSITIVITY OF MGc80-3 HUMAN GASTRIC ADENOCARCINOMA CELLS AND ITS CLINICAL SIGNIFICANCE
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作者 李学汤 林晨 李珮茵 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1990年第1期12-15,共4页
In the present study, the chemosensitivity of MGc80-3 human gastric adenocarcinoma cells was determined by means of colony-forming assay and the in vitro activities of 10 anticancer drugs were examined on the basis of... In the present study, the chemosensitivity of MGc80-3 human gastric adenocarcinoma cells was determined by means of colony-forming assay and the in vitro activities of 10 anticancer drugs were examined on the basis of the clinically achievable peak plasma drug concentration. The results showed that MGc80-3 cells were most sensitive to mitomyc'n C, adriamycin and 5-fluorouracil, being consistent with the response noted in clinical gastric cancer. This cell line may retain its original drug sensitivity and may be useful in screening for new compounds with activity against this disease. 展开更多
关键词 CHEMOSENSITIVITY OF MGc80-3 human GASTRIC ADENOCARCINOMA CELLS AND ITS CLINICAL SIGNIFICANCE PYM MTX ID BCNU
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Effects on Cell Viability and on Apoptosis in Tumoral(MCF-7)and in Normal(MCF10A)Epithelial Breast Cells after Human Chorionic Gonadotropin and Derivated-Angiotensin Peptides Treatments 被引量:1
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作者 Silvana Aparecida Alves Correa de Noronha Werica Bernardo +4 位作者 Alexandre Jesus Barros Clovis Ryuichi Nakaie Suma Imura Shimuta Ismael Dale Cotrim Guerreiro da Silva Samuel Marcos Ribeiro de Noronha 《Journal of Cancer Therapy》 2013年第7期65-69,共5页
Angiotensin-(1 - 7) [Ang-(1 - 7)] is an endogenous heptapeptide hormone of the renin-angiotensin system that has antiproliferative properties. The aim of this work was to evaluate the anti-proliferative and pro-apopto... Angiotensin-(1 - 7) [Ang-(1 - 7)] is an endogenous heptapeptide hormone of the renin-angiotensin system that has antiproliferative properties. The aim of this work was to evaluate the anti-proliferative and pro-apoptotic properties of Ang-(1 - 7) and of Ang-(1 - 7)-substituents 9-fluorenylmethyloxycarbonyl (Fmoc) e Ang II-derivatives containing the TOAC (2,2,6,6-tetramethylpiperidine-N-oxyl-4-amino-4-carboxylic acid) in normal (MCF10A) and in tumoral (MCF7) epithelial mammary cell lines. Both cell lines received an hCG and angiotensin peptides 24-hour treatment, in combination or alone followed by cell viability, apoptosis and cell cycle assays performed by flow cytometer (GUAVA). After hCG, Ang-(1 - 7), hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments, MCF7 displayed cell viability decrease and mid-apoptosis increase. We also observed cell viability decrease in MCF10A after Ang-(1 - 7), Ang-(1 - 7) Fmoc and hCG + AngII Toac treatments. These cells had an increase in late apoptosis and necrosis after AngII Toac, hCG + Ang-(1 - 7) and hCG + Ang-(1 - 7)-Fmoc treatments. Regarding the cell cycle analysis, we did not observed any changes in cell cycle phases. In summary, cell viability was decreased and apoptosis (initial, mid and late) was increased after hCG and/or Ang-(1 - 7) peptides treatments. These results point out hCG and Ang-(1 - 7) as effective compounds to inhibit cell proliferation, since they decrease cell viability and increase apoptosis in both normal and in tumoral breast cells, being the effect more pronounced in the tumoral cell line. Our results support the idea of investigating more closely the putative use of these compounds as novel therapeutic agents for breast cancer. 展开更多
关键词 Angiotensin II Angiotensin 1-7 Angiotensin II Type 1 Receptor(AT1R) Breast Cancer APOPTOSIS human Chorionic Gonadotropin
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North slope transition zone of Songnan-Baodao sag in Qiongdongnan Basin and its control on medium and large gas fields,South China Sea
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作者 XU Changgui YOU Li 《Petroleum Exploration and Development》 CSCD 2022年第6期1229-1242,共14页
Based on analysis of newly collected 3D seismic and drilled well data,the geological structure and fault system of Baodao sag have been systematically examined to figure out characteristics of the transition fault ter... Based on analysis of newly collected 3D seismic and drilled well data,the geological structure and fault system of Baodao sag have been systematically examined to figure out characteristics of the transition fault terrace belt and its control on the formation of natural gas reservoirs.The research results show that the Baodao sag has the northern fault terrace belt,central depression belt and southern slope belt developed,among them,the northern fault terrace belt consists of multiple transition fault terrace belts such as Baodao B,A and C from west to east which control the source rocks,traps,reservoirs,oil and gas migration and hydrocarbon enrichment in the Baodao sag.The activity of the main fault of the transition belt in the sedimentary period of Yacheng Formation in the Early Oligocene controlled the hydrocarbon generation kitchen and hydrocarbon generation potential.From west to east,getting closer to the provenance,the transition belt increased in activity strength,thickness of source rock and scale of delta,and had multiple hydrocarbon generation depressions developed.The main fault had local compression under the background of tension and torsion,giving rise to composite traps under the background of large nose structure,and the Baodao A and Baodao C traps to the east are larger than Baodao B trap.Multiple fault terraces controlled the material source input from the uplift area to form large delta sand bodies,and the synthetic transition belt of the west and middle sections and the gentle slope of the east section of the F12 fault in the Baodao A transition belt controlled the input of two major material sources,giving rise to a number of delta lobes in the west and east branches.The large structural ridge formed under the control of the main fault close to the hydrocarbon generation center allows efficient migration and accumulation of oil and gas.The combination mode and active time of the main faults matched well with the natural gas charging period,resulting in the hydrocarbon gas enrichment.Baodao A transition belt is adjacent to Baodao 27,25 and 21 lows,where large braided river delta deposits supplied by Shenhu uplift provenance develop,and it is characterized by large structural ridges allowing high efficient hydrocarbon accumulation,parallel combination of main faults and early cessation of faulting activity,so it is a favorable area for hydrocarbon gas accumulation.Thick high-quality gas reservoirs have been revealed through drilling,leading to the discovery of the first large-scale gas field in Baodo 21-1 of Baodao sag.This discovery also confirms that the north transition zone of Songnan-Baodao sag has good reservoir forming conditions,and the transition fault terrace belt has great exploration potential eastward. 展开更多
关键词 Qiongdongnan Basin Songnan-Baodao sag fault transition zone PaleogeneBaodo 21-1 medium and large gas fields large structural ridge composite trap hydrocarbon gas
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The Implementation Mode of No-Fly Zones in International Relations
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作者 ZHAO Guangcheng 《Cultural and Religious Studies》 2023年第12期589-599,共11页
A No-Fly Zone(NFZ)is a unique mode of humanitarian intervention which is understudied in international relations.This article focuses on the political dimension of NFZs in international relations and investigates thei... A No-Fly Zone(NFZ)is a unique mode of humanitarian intervention which is understudied in international relations.This article focuses on the political dimension of NFZs in international relations and investigates their implementations by empirically reviewing all three cases of NFZs to date.There may be efforts by intervening states to highlight humanitarian crises among certain targets to secure UN authorization for the NFZs;yet the implementation of NFZs is also driven largely by the security interests of enforcing states.As a result,there is the potential for those states implementing NFZs to transcend legal authorization and sometimes use excessive force,with likely calamitous results for local populations,or in extreme cases,accomplishing regime change by supporting opposition parties in target states. 展开更多
关键词 No-Fly zone humanitarian intervention limited use of force human rights international norm China’s diplomacy
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Effects of 4-(3-Chloro-Benzyl)-6,7-Dimethoxy-Quinazoline on Kinetics of P120-Catenin and Periplakin in Human Buccal Mucosa Squamous Carcinoma Cell Line
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作者 Isao Tamura Aiko Kamada +3 位作者 Seiji Goda Yoshihiro Yoshikawa Eisuke Domae Takashi Ikeo 《Open Journal of Stomatology》 2014年第5期249-257,共9页
In order to detect molecular markers for the epidermal growth factor inhibitor 4-(3-chloro-benzyl)- 6,7-dimethoxy-quinazoline (tyrphostin), we investigated the kinetics of p120-catenin and periplakin in the human bucc... In order to detect molecular markers for the epidermal growth factor inhibitor 4-(3-chloro-benzyl)- 6,7-dimethoxy-quinazoline (tyrphostin), we investigated the kinetics of p120-catenin and periplakin in the human buccal mucosa squamous cancer cell line BICR 10 treated with 3 nM tyrphostin. Growth of BICR 10 cells was inhibited by treatment with tyrphostin. Although changes were not observed in the expression of EGFR and p120-catenin, expression of Akt, Src and periplakin in BICR 10 treated with 3 nM tyrphostin tended to decrease. In addition, phosphorylation of EGFR, Akt and Src was inhibited by treatment with tyrphostin. On immunocytochemical staining, immunoreactions with phosphorylated EGFR, phosphorylated Akt and phosphorylated p120-catenin were weak in BICR 10 treated with tyrphostin. There was a slight immunocy to chemical reaction to periplakin in BICR 10 cells induced by tyrphostin. In conclusion, the decrease in phosphorylation in EGFR and p120-catenin by tyrphostin, following the decrease in Src or Akt phosphorylation, may inhibit expression of several growth factors associated with the proliferation and migration of cancer cells. 展开更多
关键词 4-(3-Chloro-Benzyl)-6 7-Dimethoxy-Quinazoline human Buccal Mucosa Squamous Cancer Cell Line P120-CATENIN Periplakin
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基于生态-人本融合视角的城市郊野公园规划评估与分区——以大胜关长江文化公园为例
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作者 郑吉辉 孙新旺 《西北林学院学报》 CSCD 北大核心 2024年第6期247-256,共10页
郊野公园地处城市边缘区,其功能发挥受到生态环境和人本感知的双重作用,在规划时需要采取综合性视角进行合理评估。本研究基于生态-人本融合视角,以南京市大胜关长江文化公园为例,建立生态敏感性和游客感知性综合评价指标体系,通过Arc G... 郊野公园地处城市边缘区,其功能发挥受到生态环境和人本感知的双重作用,在规划时需要采取综合性视角进行合理评估。本研究基于生态-人本融合视角,以南京市大胜关长江文化公园为例,建立生态敏感性和游客感知性综合评价指标体系,通过Arc GIS 10.6进行空间叠加处理分析,探究研究区规划分区方案并提出开发建议。结果表明:1)研究区内生态敏感性等级从低到高面积占比分别为9.10%、18.12%、23.48%、29.32%和19.98%,整体生态敏感性较高,总体上形成堤内人工湿地和堤外江滩湿地两部分,生态连续性被切割;2)研究区内游客感知性等级从低到高面积占比分别为6.14%、1.80%、7.75%、20.63%和63.68%,整体游客感知性较高,但各类型景源相对分散,且同类景源景观同质性较强;3)将研究区划分为自然恢复区、一级开发区、二级开发区、三级开发区和四级开发区5个规划分区,并提出相应规划开发建议,为我国城市郊野公园规划开发分区提供借鉴。 展开更多
关键词 郊野公园 生态敏感性 游客感知性 规划分区 生态-人本融合
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The Preparation for Human B7-2 and DC Vaccines and their Roles in Anti-tumor Immunity Against Esophageal CancerIn Vitro
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作者 Jing LU Jun ZHAO Guo-Qiang ZHAO Hong-Yan YANG You-Tian HUANGMing-Yao ZHAO Zi-Ming DONG(Department of Pathophysiology, Medical College of Zhengzhou University, Zhengzhou 450052,China) 《生物医学工程学杂志》 EI CAS CSCD 北大核心 2005年第S1期111-112,共2页
关键词 DC The Preparation for human B7-2 and DC Vaccines and their Roles in Anti-tumor Immunity Against Esophageal CancerIn Vitro APC
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Key changes across valley floors in mountain areas affected by different levels of human impact: a case study of the Ochotnica river catchment in the Polish Carpathians
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作者 Agata GOŁĄB Anna MICHNO 《Journal of Mountain Science》 SCIE CSCD 2024年第11期3567-3583,共17页
The goal of this paper was to present knowledge on changes in the morphodynamic structure of the Ochotnica River(Polish Carpathians)and transformations within its active river zone since the end of the 19th century.Th... The goal of this paper was to present knowledge on changes in the morphodynamic structure of the Ochotnica River(Polish Carpathians)and transformations within its active river zone since the end of the 19th century.The study used a set of archival and contemporary cartographic materials and the results of three-fold field mapping of the morphodynamic structure of the riverbed.Direct and indirect human interference with the natural environment of the catchment and the fluvial system was taken into account in the analysis.Analysis of changes in the morphodynamic structure of the Ochotnica and its active river zone since the end of the 19th century indicated significant changes in the development trend of the riverbed.The contemporary structure of the Ochotnica is more complex than in the 1980s.There has been a fragmentation of the riverbed into sections with different morphodynamic sections.The proportion of erosive sections has increased,which explains the reduced in the width of the active river zone of the Ochotnica.The average width of the active river zone of the river between 1861 and 2022 has changed from 80 m to 18 m.Human impact has been identified as the main reason for the changes in the morphodynamic structure of the riverbed and its active river zone.Currently,only the unregulated sect ion is characterised by free lateral migration of the riverbed and the widest active river zone(~28 m). 展开更多
关键词 Active river zone Channel changes Mountain river human impact
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