An obvious weak localization correction to anomalous Hall conductance（AHC） in very thin CoFeB film is reported.We find that both the weak localization to AHC and the mechanism of the anomalous Hall effect are relate...An obvious weak localization correction to anomalous Hall conductance（AHC） in very thin CoFeB film is reported.We find that both the weak localization to AHC and the mechanism of the anomalous Hall effect are related to the CoFeB thickness.When the film is thicker than 3 nm,the side jump mechanism dominates and the weak locaUzation to AHC vanishes.For very thin CoFeB films,both the side jump and skew scattering mechanisms contribute to the anomalous Hall effect,and the weak localization correction to AHC is observed.展开更多
The variations of ocean environmental parameters invariably result in variations of local modal wave numbers of a sound pressure field. The asymptotic Hankel transform with a short sliding window is applied to the com...The variations of ocean environmental parameters invariably result in variations of local modal wave numbers of a sound pressure field. The asymptotic Hankel transform with a short sliding window is applied to the complex sound pressure field in the water containing a mesoscale eddy to examine the variation of local modal wave numbers in such a range-dependent environment. The numerical simulation results show that modal wave number spectra obtained by this method can reflect the location and strength of a mesoscale eddy, therefore it can be used to monitor the strength and spatial scale of ocean mesoscale eddies.展开更多
Gene expression quantification at mRNA level is very important for postgenomic studies, as gene expression level is the reflection of the special biological function of the target gene. Methods used for gene expressio...Gene expression quantification at mRNA level is very important for postgenomic studies, as gene expression level is the reflection of the special biological function of the target gene. Methods used for gene expression quantification, such as microarray or quantitative real-time polymerase chain reaction （qRT-PCR）, require stable expressed reference genes. Thus, finding suitable control genes is essential for gene quantification. In this study, a genome-wide survey of reference genes during metamorphism was performed on silkworm Bombyx mori. Twelve genes were chosen as putative reference genes based on a whole genome oligonucleotide microarray normalized by external controls. Then, qRT- PCR was employed for further validation and selection of potential reference gene candidates. The results were analyzed, and stable genes were selected using geNorm 3.4 and NormFinder software. Finally, considering factors from every aspect, translation initiation factor 4A, translation initiation factor 3 subunit 4, and translation initiation factor 3 subunit 5 （represented by sw22934, sw14876, and sw13956） were selected as reliable internal controls across the examined developmental stages, while cytoplasmic actin （sw22671）, the commonly used reference gene in a previous study was shown to vary drastically throughout the examined developmental stages. For future research, we recommend the use of the geometric mean of those three stable reference genes as an accurate normalization factor for data normalization of different developmental stages during metamorphism.展开更多
基金supported by the National Basic Research Program of China(Grant No.2012CB933102)the National Natural Science Foundation of China(Grant Nos.11079052,11174354,and 51172080)
文摘An obvious weak localization correction to anomalous Hall conductance（AHC） in very thin CoFeB film is reported.We find that both the weak localization to AHC and the mechanism of the anomalous Hall effect are related to the CoFeB thickness.When the film is thicker than 3 nm,the side jump mechanism dominates and the weak locaUzation to AHC vanishes.For very thin CoFeB films,both the side jump and skew scattering mechanisms contribute to the anomalous Hall effect,and the weak localization correction to AHC is observed.
文摘The variations of ocean environmental parameters invariably result in variations of local modal wave numbers of a sound pressure field. The asymptotic Hankel transform with a short sliding window is applied to the complex sound pressure field in the water containing a mesoscale eddy to examine the variation of local modal wave numbers in such a range-dependent environment. The numerical simulation results show that modal wave number spectra obtained by this method can reflect the location and strength of a mesoscale eddy, therefore it can be used to monitor the strength and spatial scale of ocean mesoscale eddies.
文摘Gene expression quantification at mRNA level is very important for postgenomic studies, as gene expression level is the reflection of the special biological function of the target gene. Methods used for gene expression quantification, such as microarray or quantitative real-time polymerase chain reaction （qRT-PCR）, require stable expressed reference genes. Thus, finding suitable control genes is essential for gene quantification. In this study, a genome-wide survey of reference genes during metamorphism was performed on silkworm Bombyx mori. Twelve genes were chosen as putative reference genes based on a whole genome oligonucleotide microarray normalized by external controls. Then, qRT- PCR was employed for further validation and selection of potential reference gene candidates. The results were analyzed, and stable genes were selected using geNorm 3.4 and NormFinder software. Finally, considering factors from every aspect, translation initiation factor 4A, translation initiation factor 3 subunit 4, and translation initiation factor 3 subunit 5 （represented by sw22934, sw14876, and sw13956） were selected as reliable internal controls across the examined developmental stages, while cytoplasmic actin （sw22671）, the commonly used reference gene in a previous study was shown to vary drastically throughout the examined developmental stages. For future research, we recommend the use of the geometric mean of those three stable reference genes as an accurate normalization factor for data normalization of different developmental stages during metamorphism.
