Association of Phenotypic and Genotypic Fluoroquinolone Susceptibility among Non-Typhoidal Salmonella Isolates from Jeddah, KSA

Non-typhoid Salmonella (NTS) infects 250 to 3200 per 100,000 individuals from all over the world. NTS infection is relatively high in Jeddah (ranges between 44-132/100,000 population) as compared to other cities of Saudi Arabia. NTS isolates have also shown increasing resistance to conventional antibiotics. Therefore, fluoroquinolone (FQ) is considered drug of choice for the treatment of invasive NTS infections. A rapid detection of FQ resistance may greatly assist in appropriate therapy and containment of resistant NTS strains. Thus, molecular detection of mutations in FQ resistance genes (gyrA and parC) may play a promising role. Since limited data were available about FQ resistance among NTS isolates, therefore, this study primarily explored the occurrence of phenotypic and genotypic FQ resistance among NTS isolates from Jeddah, Saudi Arabia. Study also explored any correlation between phenotypic and genotypic FQ resistance. Fifty NTS isolates were collected from a public sector hospital of Jeddah from January to December, 2014. FQ susceptibility was determined for 48 NTS isolates using Kirby-bauer disk diffusion method and results were interpreted according to Clinical and Laboratory Standards Institute (CLSI) methodology. Genotypic resistance to FQ was determined by exploring mutations in gyrA and parC genes using PCR- based gene-sequencing method. Results were statistically analyzed using Social Package of Statistical Science (SPSS) version 22. Phenotypic antibiogram revealed that 38% (18/48) NTS isolates were FQ resistant, that 23% (11/48) were intermediately susceptible and that 39% (19/48) were susceptible. Genotypic resistance revealed mutations in only four codons of gyrA and parC genes among 39% (7/18) of FQ resistant isolates. 43% (3/7) of FQ resistant isolates showed mutations at two codons 83 (S83F, S83Y) and 87, (D87G, D87Y, D87W) of gyrA gene. Two resistant isolates showed triple mutations i.e. at codons 83 and 87 of gyrA and codon 80 (S80I and S80W) of parC gene, while one resistant isolate revealed mutation at codon 87 of gyrA and 57 (S57T) of parC gene. Moreover, 55% (6/11) intermediately susceptible isolates for FQ also revealed mutation at codon 83 of gyrA gene whereas;one intermediately susceptible isolate (1/6) also revealed additional mutation at codon 57 of parC gene. None of the FQ susceptible NTS isolates showed any mutations in gyrA or parC genes. Occurrence of mutations at only four codons in gyrA and parC genes among FQ resistant isolates may assist in development of rapid molecular method for FQ resistance detection. Presence of mutations among more than fifty percent of intermediately susceptible FQ isolates is alarming and may serve as a predictor for pre-resistant isolates for FQ. Moreover, absence of mutation in about sixty percent of phenotypically FQ resistant NTS isolates shows existence of an alternate resistance mechanism requiring further investigations.

肥胖儿童non-HDL-C、remnant cholesterol与亚临床动脉粥样硬化的关系

目的:了解non-HDL-C和remnant cholesterol与肥胖儿童亚临床动脉粥样硬化(AS)的关系。方法:根据甘油三酯(TG)水平将65例肥胖儿童(肥胖组)分为I组(TG≥2.3 mmol/L)32例,II组(TG<2.3 mmol/L)33例,与肥胖组儿童性别年龄相匹配的正常体质量儿童30例为III组(对照组)。比较3组儿童血脂指标,对肥胖组各项血脂指标与血浆致动脉粥样硬化指数(AIP)进行相关性分析,肥胖患儿体质指数、血脂指标与颈动脉内径、内膜-中层厚度(c-IMT)、血流参数进行相关性分析。结果:I组、II组的TG、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、non-HDL-C、remnant cholesterol、AIP均较对照组升高,高密度脂蛋白胆固醇(HDL-C)较对照组降低,且I组的remnant cholesterol、AIP均较II组升高。肥胖组儿童AIP与TG、non-HDL-C、remnant cholesterol呈正相关,与HDL-C呈负相关。37例行颈动脉超声检查的肥胖儿童的c-IMT较正常儿童的增厚。结论:non-HDL-C、remnant cholesterol在肥胖儿童亚临床AS中有重要作用,在肥胖儿童血脂异常的治疗中应关注non-HDL-C、remnant cholesterol的变化。

Shenqi Fuzheng injection combined with GP chemotherapy in the treatment of advanced non-small cell lung cancer： a meta-analysis

Objective： To evaluate the clinical efficacy of Shenqi Fuzheng injection combined with gemcitabine plus cisplatin（GP） in the treatment of advanced non-small cell lung cancer （NSCLC）. Methods： we performed a systematicsearch in the electronic databases such as Cochrane Library, Pubmed, Embase, Chinese Journal Full-text Database,Chinese Biomedical Literature Database, Chinese Science and Technology Periodical Full-text Database andWanfang Database up to 30 January 2017. Randomized controlled trials （RCT） of Shenqi Fuzheng Injectioncombined with GP chemotherapy in the treatment of advanced NSCLC were searched, and all the RCTs wereconducted on methodological quality assessment. Data extraction and data analysis were according to standards ofCochrane systematic review. Results： Eight trials were included including a total of 701 patients. Meta-analysisresults： Shenqi Fuzheng injection combined with GP chemotherapy could significantly improve the functionalstatus of patients with NSCLC （OR = 3.44, 95% CI [2.26, 5.25], P 〈 0.0001） and clinical treatment efficacy （OR =（OR = 0.31, 95%CI [0.20, 0.47], P 〈 0.0001. The rate of leukopenia （OR = .31, 95%CI [0.20,0.47], P 〈 0.0001）,thrombocytopenia （OR = 0.58, 95%CI [0.37, 0.91], P = 0.020）, hemoglobin decline （（OR = 0.31, 95%CI [0.16,0.59], P = 0.0004） and incidence of gastrointestinal reactions （OR = 0.58,P 〈 0.05） could be reduced. Conclusion：Shenqi Fuzheng injection combined with GP chemotherapy in the treatment of advanced NSCLC obtainedsignificantly clinical efficacy. The quality of the literature incorporated is low, the conclusion requires high-qualityresearch to further prove.

Analysis of Numerical Results in High Temperature Congealment and Chemistry Non-equilibrium Flow Field

Using the air plasma ignition technique, physicochemical process of burning can be accelerated; concentration limit ofretrofires both can be extended; reliability of retrofires and stability of burning can be improved. In this paper, using internalequivalent heat area in place of electric are that created Ohm heat, the flow fields of thermodynamic equilibrium chemistry con-gealment and chemistry non-equilibrium in the plasma generator were simulated. The influences of the inlet prerotation angleof air, the inlet total pressure of air and the airflow compression angle of spray nozzle on the temperature on the surface of elec-

Turbulent Flow Action of Pulp in Wet - Laid Non - Woven Processes

The paper deals with the fluid field of web forming in wet-laid non-woven production.The influence of the turbulent flow on blending fiber and occluded fluid produced in pulp flow has been discussed in theory and practice.The suitable use of the imported velocity of pulp is very important in producing wet-laid products of good quality.

CYFRA 21-1 as an early predictor of first line chemotherapy response in advanced non small cell lung cancer

Objective： In an era of ever evolving, promising new therapies for advanced non small cell lung cancer （NSCLC）, early predictors of response to therapy, are needed. We evaluated early variations in CYFRA 21-1 serum levels of patients with advanced NSCLC receiving first line chemotherapy and correlated the results with objective tumor response. Methods： 29 consecutive, previously untreated, patients of advanced non small cell lung cancer, with measurable disease on CT scan were evaluated. All patients were treated with conventional systemic chemotherapy, although the choice of chemotherapy was left to the discretion of the treating physicians. Serum samples were obtained immediately before the start of 1st and 2nd cycles of chemotherapy. CYFRA 21-1 was measured with an electrochemiluminescense immunoassay on an automatic analyzer （Elecsys 2000; Roche Diagnostics）. Response was evaluated using Response evaluation criteria in solid tumors （RECIST） criteria. Results： 10 patients had partial response, 9 patients had stable disease and 9 had progressive disease. None of the patients had complete response. 21/29 （72%） patients had an elevated baseline value of CYFRA 21-1.62% patients （18/29） had a decrease in CYFRA 21-1 after 1 cycle of chemotherapy. The average reduction in the 2nd reading was irrespective of whether baseline value was normal or not. The average reduction was statistically significant （P = 0.002; 95% CI, from 0.8369 to 3.49464; t test）. 8 out of 10 （80%） patients with partial response had a reduction in their 2nd reading of. CYFRA （P = 0.019; 95% CI, from 0.81965 to 7.20035; t test） which was significant. We also observed that 6/9 （66%） patients whose disease remains stable also had a decrease in their subsequent reading （P = 0.0106; 95% CI, from -0.44942 to 3.82720; t test）, though it was not significant statistically. Although 5 out of 9 （55%） patients, who had an increase in their CYFRA 21-1 level, had progressive disease, but it was not statistically significant （P = 0.537; 95% CI, from -1.20021 to 2.13354; ttest）. 14 out of 19 （73%） who either had partial response or had stable disease, had a reduction in their 2nd value of CYFRA 21-1 and was significant statistically （P = 0.004; 95% CI, from 0.74792 to 3.50208; t test）. We also observed that except for 1 patient, all patients who had a decrease of 42% or more in their subsequent CYFRA 21-1 level, were those who had either responded to chemotherapy or had stable disease （P = 0.001）, which was statistically significant. Conclusion： We can conclude that monitoring of serum marker CYFRA 21-1, early dudng first-line chemotherapy may be a useful prognostic tool for evaluation of early tumor response in patients with advanced NSCLC.

MiR-16-5p plays an inhibitory role in human non-small cell lung cancer through Fermitin family member 2

Increasing evidence indicates that aberrant expressions of some microRNAs are associated with cancer progression.However,the roles and biological mechanisms of miRNA-16-5p in human non-small cell lung cancer(NSCLC)are not to be well studied.Here,we validated that the expression of miR-16-5p was decreased significantly in NSCLC samples and cell lines.The correlation between the clinicopathological features of NSCLC and the miR-16-5p expression showed that the expression of miR-16-5p in non-small cell lung cancer was linked with the advanced TNM stage,positive lymph node metastasis,with short overall survival(OS).Also,a negative correlation between miR-16-5p and Fermitin family member 2(FERMT2)was observed,implying there may be a potential link about their regulation.The hypothesis was further confirmed by in-silico analysis and dual-luciferase reporter assay.Moreover,we demonstrated that the transfections of miR-16-5p mimics could alter some biological characteristics of NSCLC cells remarkably accomplished by the expression variance of FERMT2 in vitro and in vivo assays.Summarily,this study demonstrated that miR-16-5p,as a tumor suppression factor in NSCLC by targeting FERMT2,could serve as one promising biomarker in the prediction for NSCLC patients.

FAREY SEQUENCE OF NONLINEAR DIFFERENTIAL DYNAMIC SYSTEM IN TWO DIMENSIONS

In this paper , through the discrim ination of Farey sequence in the forced Brusselator withweak coupling , it is proved that there is a topological translation fro m a nonlinear differen tial system ( limit cycle) to the circle m ap .

Ways to Overcome Anxiety in the English Listening Class in College (SUN JING-YU KE SU-FEN)

In my ten-yeat experience of tenching English as major in college , I feel that students experience more difficulties in listening lessons than in other lessons. What, then, prevents students from achieving success in the listening class? I suggest that anxiety is one of the major factors, which interferes with students listening comprehension. And I would agree with writers such as Gardner and Horwitz et al. (1991)that anxiety may impede learner’s ability to perform successfully in all aspects of learning a foreign language. I feet it is particularly significant for listening.

氨基酸与钠离子非共价键相互作用的电喷雾质谱研究

实验研究中常常观察到钠离子与一些多肽或蛋白质有较强的非共价键结合能力,这种非共价键相互作用可能与生物的一些功能和活性有关.为了进一步了解这一结合作用,我们应用电喷雾质谱研究了常见的20种氨基酸与钠离子在溶液中的非共价键相互作用.实验研究中发现脯氨酸和苯丙胺酸与钠离子有较强的非共价键结合能力.……

7株非结核分枝杆菌对氟喹诺酮类药物的敏感性分析

目的:探讨不同种类非结核分枝杆菌对于氟喹诺酮类药物的敏感性。方法:收集某医院保存的7株非结核分枝杆菌,通过16SrRNA、rpoB、hsp65基因测序的方法,7株非结核分枝杆菌分别鉴定为脓肿分枝杆菌3株,堪萨斯分枝杆菌3株,塞内加尔分枝杆菌1株。用微量肉汤稀释法检测其对环丙沙星、氧氟沙星、左氧氟沙星的最小抑菌浓度。结果:脓肿分枝杆菌对3种氟喹诺酮类药物耐药,最低抑菌浓度(MIC)值均≥16μg/mL;塞内加尔分枝杆菌分离株对氧氟沙星和环丙沙星耐药,MIC值均为4μg/mL。对左氧氟沙星敏感,MIC值为2μg/mL。2株堪萨斯分枝杆菌在MHB培养基中未生长,另1株堪萨斯分枝杆菌对氧氟沙星不耐药,MIC值为1μg/mL,对左氧氟沙星和环丙沙星敏感,MIC值分别为0.125μg/mL和0.25μg/mL。结论:本组临床分离株中,脓肿分枝杆菌的耐药程度高于堪萨斯分枝杆菌和塞内加尔分枝杆菌。3种药物中非结核分枝杆菌对左氧氟沙星的敏感度高于环丙沙星和氧氟沙星。

新型喹诺酮抗菌药——T3811

本文介绍了一种非氟喹诺酮抗菌新药—T 3811,综述了其体外抗菌活性,并与环丙沙星、左氧氟沙星和曲伐沙星进行了比较。此外,还提出了将其作为兽药开发应用的潜在可能性。

PROGRESS IN ION BEAM ANALYSIS AT FUDAN UNIVERSITY

Progres in ion beam analysis at Fudan University in the recent years is briefly reviewed. Presented as examples of the research activities performed in this field are the following projects: (1) Nuclear potential resonance scattering of 6.25 MeV and 4.25 MeV helium ions for simultaneous compositional analysis of carbon and oxygen in a Mylar, a SnInO, and some other film samples: (2) Determination of stoichiometry of a high-temperature superconducting Y-Ba-Cu-O sample by backscattering of 8.8 MeV helium ions; (3) Backscattering and channeling analysis of multilayered structures periodically consisting of layers of pure Si and alternate layers of Ge and Si, grown on (100) Si substrates by molecular beam epitaxy: (4) Studies of surface structure of Al(100) by the use of MeV ions backscattering and channeling surface peak: and (5) MeV ion microbeam analysis and the use of PIXE method in DNA study. etc.

Optically Powered Temperature Measuring Instrument for Big Rotor

Abstract: A micro - power consumption non - contact temperature measuring instrument for big rotor is introduced. As it solves very well the signal coupling under high speed rotation and power supply problem for probe, the instrument can realize persistent on - line temperature measurement for big rotor drived by the ordinary light transmitted by optical fiber under the room light.

Heat Production Rate Calculation of Thermal Manikin with a Compensation Method

Thermal manikin plays important roles in simulating thethermal state of human bodies to facilitate the evaluationof thermal comfort properties of various clothing assem-blies.Based on the heat balance principle and analysis ofrelated factors,the heat production rate of manikin isrecommended as an efficient evaluation index.Whereas,its inside heat production which occurs as a result of theexistence of temperature difference between its insidepart and outside surface,should not be ignored.Through a series of theoretical analysis and calculations,a compensative equation is deduced in this paper.

EFFECT OF STRUCTURE PARAMETERS OF POLYESTER ON ITS ALKALI REACTION RATE

The effect of the supermoleclar structure of polyester on the effective alkali hy-drolysisis rate constant k has been investigated in the paper.That the average non-crvstal line volume around per crystal grain has a close relation to the k is proved byexperiments.A simple and effctive method for assessing what effect of the super-molecular structure on the k by its CDT value( Critical Dissolution Time) has beenput forward.

Some Problems Concerning Geologic Structure with Double Layers in Urban Underground Lifeline Engineering

Underground lifeline engineering (ULE for short) in modern city demands the appreciation of an active fault in buried bedrock . Generally speaking , a large number of urban geological textures of a basement may all be simplified into a dual geological texture model , i. e., the upper part of the basement consists of loose covering layer and the lower part consists of bedrock . The study of an active fault should include three parts of contents , i . e ., to determine the lower time limit of activity of the fault , and the time limit must be recognized by both of designing engineers and geologists ; on the basis of the studies of repetition periods of earthquake occurrence to deter mine whether the fault moves or not during the allowed time of efficacy of buildings and constructions ; for the sake of engineering practice , the active rate of the fault must be given . The fault with different active mechanism has different effects on the ULE . The authors studied the effect of lateral non-uniform overburden site on the ULE by means of the supersonic earthquake modelling . Owing to the lateral non - uniformity of the covering sediments , there occurs an obvious jump of amplitude of the seismic wave propagation near the contact surface between two different sedi ments . In addition , from the modelling experiment curves it may be seen that the different focus mechanisms and different medium characters may also exert an effect in different degrees .

Substituting Effect of Y in La_(0.5) Ba_(0.5) CoO_3

La 0.5- x Y x Ba 0.5 CoO 3 polycrystals were prepared by solid state reaction. The substituting effects of Y for La on the magnetic and transport properties of the materials were studied systematically. The results indicate that substitution of Y induces two effects. Firstly, the charge transfer from Y to 3d orbital of Co happens. This causes the molecular magnetic moment to decrease. Secondly, the antiferromagnetic exchange interaction of Co ions appears. When the content of Y is less than or equal to 30%, the non colinear structure of spins in materials is observed. When the content of Y is greater than 30%, the materials transit from predominant ferromagnetic state to predominant antiferromagnetic one. The conductive mechanism for the materials with different content of Y belongs to the variable range hopping conduction of polarons. The resistivity of materials increases sharply with increasing Y content.

临床分离布伦登卢普沙门菌的分子分型和对氟喹诺酮耐药机制

目的 研究布伦登卢普沙门菌分子分型特点和对氟喹诺酮耐药机制。方法 对2012-2014年天津市两家教学医院(天津医科大学第二医院和天津医科大学总医院)肠道门诊就诊的急性腹泻患者进行粪便培养,分离非重复布伦登卢普沙门菌,并采用脉冲场凝胶电泳(PFGE)分型和多位点序列分型(MLST),聚合酶链式反应(PCR)扩增氟喹诺酮靶位基因(parC、parE、gyrA、gyrB)的耐药决定区(QRDR)和质粒介导的喹诺酮耐药(PMQR)基因[qepA、oqxAB、acc(6′)-Ib-cr、qnrA、qnrB、qnrC、qnrD和qnrS],对阳性扩增产物测序,采用Blast进行序列分析。结果 共得到8株布伦登卢普沙门菌,PCR-DNA序列分析显示,菌株均为ST22型,且7个管家基因hisD、purE、hemD、aroC、sucA、dnaN、thrA的等位基因谱完全一致;PFGE分型显示,A型7株,酶切条带100%一致,B型1株,与A型相似度为97.3%;菌株的gyrA基因QRDR位于第87位的氨基酸发生了改变,即单点有义突变,而PMQR基因扩增均阴性;最小抑菌浓度(MIC)法显示,所有的菌株对氟喹诺酮中介耐药,K-B法检测显示只有一半的菌株对环丙沙星敏感。结论 克隆相关的氟喹诺酮耐药的ST22型布伦登卢普沙门菌在本地区持续出现,应警惕存在暴发感染的可能。K-B法会漏检环丙沙星中介耐药的NTS菌株,临床需加以注意。