利用Triton X-100提升巴氏芽孢杆菌脲酶活性的效果

微生物诱导碳酸盐沉积(MICP)技术是近年来发展起来的一种新型绿色加固技术,如何提升MICP技术的加固效能一直是学者关注的热点,本工作重点结合巴氏芽孢杆菌产脲酶原理和非离子表面活性剂的特点,设计采用非离子表面活性剂Triton X-100提升MICP效能的试验研究。结果表明:(1)在单因素试验结果的基础上,通过响应面试验分析确定了活性剂的最优处理条件,即掺量1.37%、处理时间5.02 h、处理温度34.28℃、菌胶比1∶2.11;(2)采用最优条件处理的巴氏芽孢杆菌对钙质砂进行加固后,钙质砂固化体碳酸钙含量和无侧限抗压强度分别提升了25.48%和22.83%;(3)非离子表面活性剂Triton X-100增大了巴氏芽孢杆菌的细胞膜通透性,一方面使得更多的胞内脲酶释放到细胞外,同时更多的尿素分子进入到细胞内,促进了尿素水解,提升了碳酸钙的生成速率;另一方面使得以细菌为核点生成的碳酸钙胶凝体的结构更为致密,在这两方面作用下,MICP效能得到有效提升。相关思路和研究结果可为MICP加固效果的提升提供新的参考和思路。

非离子改性水性醇酸树脂-丙烯酸树脂杂化体的制备及性能

首先用新戊二醇、间苯二甲酸–5–磺酸钠合成磺酸盐聚酯中间体,然后将其和非离子亲水单体(N120)、季戊四醇、豆油酸、邻苯二甲酸酐进行聚酯化反应合成水性醇酸树脂。再将水性醇酸树脂用丙烯酸酯单体进行自由基接枝聚合得到非离子改性水性醇酸树脂–丙烯酸树脂杂化体。用傅里叶变换红外光谱(FT-IR)、热重分析(TGA)对其结构进行了表征;探究了非离子亲水单体的用量、醇酸树脂分子量、油度、酸值对杂化体成膜性能和贮存稳定性的影响。结果显示:非离子亲水单体占水性醇酸树脂4%~8%(质量分数)、醇酸树脂分子量为2 200~2 500,油度为50%、杂化体酸值为10 mg KOH/g时制得的杂化体涂膜耐水性、干性、光泽及贮存稳定性最好。

Liquid-liquid Extraction System Based on Non-ionic Surfactant-salt-H_2O and Mechanism of Drug Extraction

Extraction behavior of chlorpromazine hydrochloride (CPZ) and procaine hydro- chloride (PCN) in the system described in the title was studied. Research shows that the extraction efficiency of CPZ can amount to 96% by twice extraction, while that of PCN is 77%. This system produces the distribution coefficients (KD) of 12.3 and 2.6 respectively for CPZ and PCN. Extraction mechanism is deduced according to ultraviolet and molecular fluorescence spectra variation of the drugs in the system studied.

STUDY ON NEUTRAL DEINKING OF ONP WITH NON-IONIC SURFACTANTS

The ONP neutral deinking performances of fatty alcohol polyoxyethylene ether with different EO value were investigated in this paper. Meanwhile, the synergistic effects of different non-ionic surfactants, the co-operation of non-ionic surfactants with anionic surfactants,and the effects of different salts added into the above two systems on deinkability were also studied. The results showed the deinking performance of A7 was good. But the synergistic effect of A7 and A4 was better. In addition, the accession of salt W2 could improve the deinking efficiency, and the brightness of the deinked pulp was 1.0%ISO higher than that of A7 and A4.

A Simple and Rapid Method for the Detection of Non-Ionic Surfactants

A spectrophotometric approach for the detection of non-ionic surfactant (Triton X-100) has been proposed in this paper. This method does not involve extraction of the ion-associate with harmful solvents, but employs adhesion of the ion-association of potassium/non-ionic surfactants complex and tetraphenylporphyrin tetrasulfonic acid obtained by vigorous shaking. The adhered ion-associate was dissolved with water and its absorbance was measured. The sensitivity for Triton X-100 was determined to be 0.146 (expressed as absorbance of 1 mg/L solution). The adhesion tendency of ion-associate was found to be dependent on the water contact angle, which in turn was influenced by a high adhesion of the ion-associate and by low blank values. In this respect, a tetrafluo-roethylene vessel was found to be the most suitable for the detection of non-ionic surfactants. This spectrophotometrical method is simply and rapidly performed by a procedure based on mechanical shaking and can be employed to detect non-ionic surfactants containing more than 7 polyethylene oxide units.

Ionic Conduction and Fuel Cell Performance of Ba0.98Ce0.8Tm0.2O3-α Ceramic

The perovskite-type oxide solid solution Ba0.98Ce0.8Tm0.2O3-α was prepared by high temperature solid-state reaction and its single phase character was confirmed by X-ray diffraction. The conduction property of the sample was investigated by alternating current impedance spectroscopy and gas concentration cell methods under different gases atmospheres in the temperature range of 500-900 ℃. The performance of the hydrogen-air fuel cell using the sample as solid electrolyte was measured. In wet hydrogen, the sample is a pure protonic conductor with the protonic transport number of 1 in the range of 500-600 ℃, a mixed conductor of proton and electron with the protonic transport number of 0.945-0.933 above 600 ℃. In wet air, the sample is a mixed conductor of proton, oxide ion, and electronic hole. The protonic transport numbers are 0.010-0.021, and the oxide ionic transport numbers are 0.471-0.382. In hydrogen-air fuel cell, the sample is a mixed conductor of proton, oxide ion and electron, the ionic transport numbers are 0.942 0.885. The fuel cell using Ba0.98Ce0.8Tm0.2O3-α as solid electrolyte can work stably. At 900 ℃, the maximum power output density is 110,2 mW/cm2, which is higher than that of our previous cell using Ba0.98Ce0.8Tm0.2O3-α （x〈≤1, RE=Y, Eu, Ho） as solid electrolyte.

肥胖儿童non-HDL-C、remnant cholesterol与亚临床动脉粥样硬化的关系

目的:了解non-HDL-C和remnant cholesterol与肥胖儿童亚临床动脉粥样硬化(AS)的关系。方法:根据甘油三酯(TG)水平将65例肥胖儿童(肥胖组)分为I组(TG≥2.3 mmol/L)32例,II组(TG<2.3 mmol/L)33例,与肥胖组儿童性别年龄相匹配的正常体质量儿童30例为III组(对照组)。比较3组儿童血脂指标,对肥胖组各项血脂指标与血浆致动脉粥样硬化指数(AIP)进行相关性分析,肥胖患儿体质指数、血脂指标与颈动脉内径、内膜-中层厚度(c-IMT)、血流参数进行相关性分析。结果:I组、II组的TG、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、non-HDL-C、remnant cholesterol、AIP均较对照组升高,高密度脂蛋白胆固醇(HDL-C)较对照组降低,且I组的remnant cholesterol、AIP均较II组升高。肥胖组儿童AIP与TG、non-HDL-C、remnant cholesterol呈正相关,与HDL-C呈负相关。37例行颈动脉超声检查的肥胖儿童的c-IMT较正常儿童的增厚。结论:non-HDL-C、remnant cholesterol在肥胖儿童亚临床AS中有重要作用,在肥胖儿童血脂异常的治疗中应关注non-HDL-C、remnant cholesterol的变化。

Oxygen ionic conductivity of a composite electrolyte SDC-LSGM prepared via glycine-nitrate process

Ce0.8Sm0.2O1.9-δ-La0.9Sr0.1Ga0.8Mg0.2O3-δ（SDC-LSGM）is prepared by the glycine-nitrate process（GNP）.SDC-LSGM composite electrolyte samples with different weight ratios are prepared by the co-combustion method so as to obtain homogeneous nano-sized precursor powders. The X-ray diffraction （XRD） and the scan electron microscope （SEM） are used to investigate the phases and microstructures. The measurements and analyses of oxygen ionic conductivity of SDC-LSGM are carried out through the four-terminal direct current （DC） method and the electrochemical impendence spectroscopy, respectively. The optimum weight ratio of SDC-LSGM is 8∶2, of which the ionic conductivity is 0.113 S/cm at 800℃ and the conductivity activation energy is 0.620 eV. The impendence spectra shows that the grain boundary resistance becomes the main barrier for the ionic conductivity of electrolyte at lower temperatures. The appropriate introduction of LSGM to the electrolyte SDC can not only decrease the electronic conductivity but also improve the conditions of the grain and grain boundary, which is advantageous to cause an increase in oxygen ionic conductivity.

非离子表面活性剂Triton X-100/Na_(2)SO_(4)双水相体系萃取分离钨钼的研究

采用非离子表面活性剂Triton X-100与硫酸钠构成的双水相体系,研究了水溶液中钨和钼的萃取分离性能。考察了温度、pH值、钼络合剂种类及用量等对钨和钼分离性能的影响。结果表明:在酸性条件下,络合剂酒石酸钠、柠檬酸钠能与钼形成稳定的络合物、从而抑制钨钼杂多酸的形成,实现钨、钼的高效分离;在pH=2.0,温度为343.15K,络合剂酒石酸钠质量分数为3%时,钨、钼分离效果最好,分离因子可达328。借助紫外光谱、红外光谱等分析手段研究了钨、钼萃取机理。结果表明,钨依靠仲钨酸B阴离子与表面活性剂分子中质子化的聚氧乙烯拟阳离子间产生的静电引力被萃取到表面活性剂相,而钼则以亲水性络合物形式留在水相。

Shenqi Fuzheng injection combined with GP chemotherapy in the treatment of advanced non-small cell lung cancer： a meta-analysis

Objective： To evaluate the clinical efficacy of Shenqi Fuzheng injection combined with gemcitabine plus cisplatin（GP） in the treatment of advanced non-small cell lung cancer （NSCLC）. Methods： we performed a systematicsearch in the electronic databases such as Cochrane Library, Pubmed, Embase, Chinese Journal Full-text Database,Chinese Biomedical Literature Database, Chinese Science and Technology Periodical Full-text Database andWanfang Database up to 30 January 2017. Randomized controlled trials （RCT） of Shenqi Fuzheng Injectioncombined with GP chemotherapy in the treatment of advanced NSCLC were searched, and all the RCTs wereconducted on methodological quality assessment. Data extraction and data analysis were according to standards ofCochrane systematic review. Results： Eight trials were included including a total of 701 patients. Meta-analysisresults： Shenqi Fuzheng injection combined with GP chemotherapy could significantly improve the functionalstatus of patients with NSCLC （OR = 3.44, 95% CI [2.26, 5.25], P 〈 0.0001） and clinical treatment efficacy （OR =（OR = 0.31, 95%CI [0.20, 0.47], P 〈 0.0001. The rate of leukopenia （OR = .31, 95%CI [0.20,0.47], P 〈 0.0001）,thrombocytopenia （OR = 0.58, 95%CI [0.37, 0.91], P = 0.020）, hemoglobin decline （（OR = 0.31, 95%CI [0.16,0.59], P = 0.0004） and incidence of gastrointestinal reactions （OR = 0.58,P 〈 0.05） could be reduced. Conclusion：Shenqi Fuzheng injection combined with GP chemotherapy in the treatment of advanced NSCLC obtainedsignificantly clinical efficacy. The quality of the literature incorporated is low, the conclusion requires high-qualityresearch to further prove.

Analysis of Numerical Results in High Temperature Congealment and Chemistry Non-equilibrium Flow Field

Using the air plasma ignition technique, physicochemical process of burning can be accelerated; concentration limit ofretrofires both can be extended; reliability of retrofires and stability of burning can be improved. In this paper, using internalequivalent heat area in place of electric are that created Ohm heat, the flow fields of thermodynamic equilibrium chemistry con-gealment and chemistry non-equilibrium in the plasma generator were simulated. The influences of the inlet prerotation angleof air, the inlet total pressure of air and the airflow compression angle of spray nozzle on the temperature on the surface of elec-

Turbulent Flow Action of Pulp in Wet - Laid Non - Woven Processes

The paper deals with the fluid field of web forming in wet-laid non-woven production.The influence of the turbulent flow on blending fiber and occluded fluid produced in pulp flow has been discussed in theory and practice.The suitable use of the imported velocity of pulp is very important in producing wet-laid products of good quality.

CYFRA 21-1 as an early predictor of first line chemotherapy response in advanced non small cell lung cancer

Objective： In an era of ever evolving, promising new therapies for advanced non small cell lung cancer （NSCLC）, early predictors of response to therapy, are needed. We evaluated early variations in CYFRA 21-1 serum levels of patients with advanced NSCLC receiving first line chemotherapy and correlated the results with objective tumor response. Methods： 29 consecutive, previously untreated, patients of advanced non small cell lung cancer, with measurable disease on CT scan were evaluated. All patients were treated with conventional systemic chemotherapy, although the choice of chemotherapy was left to the discretion of the treating physicians. Serum samples were obtained immediately before the start of 1st and 2nd cycles of chemotherapy. CYFRA 21-1 was measured with an electrochemiluminescense immunoassay on an automatic analyzer （Elecsys 2000; Roche Diagnostics）. Response was evaluated using Response evaluation criteria in solid tumors （RECIST） criteria. Results： 10 patients had partial response, 9 patients had stable disease and 9 had progressive disease. None of the patients had complete response. 21/29 （72%） patients had an elevated baseline value of CYFRA 21-1.62% patients （18/29） had a decrease in CYFRA 21-1 after 1 cycle of chemotherapy. The average reduction in the 2nd reading was irrespective of whether baseline value was normal or not. The average reduction was statistically significant （P = 0.002; 95% CI, from 0.8369 to 3.49464; t test）. 8 out of 10 （80%） patients with partial response had a reduction in their 2nd reading of. CYFRA （P = 0.019; 95% CI, from 0.81965 to 7.20035; t test） which was significant. We also observed that 6/9 （66%） patients whose disease remains stable also had a decrease in their subsequent reading （P = 0.0106; 95% CI, from -0.44942 to 3.82720; t test）, though it was not significant statistically. Although 5 out of 9 （55%） patients, who had an increase in their CYFRA 21-1 level, had progressive disease, but it was not statistically significant （P = 0.537; 95% CI, from -1.20021 to 2.13354; ttest）. 14 out of 19 （73%） who either had partial response or had stable disease, had a reduction in their 2nd value of CYFRA 21-1 and was significant statistically （P = 0.004; 95% CI, from 0.74792 to 3.50208; t test）. We also observed that except for 1 patient, all patients who had a decrease of 42% or more in their subsequent CYFRA 21-1 level, were those who had either responded to chemotherapy or had stable disease （P = 0.001）, which was statistically significant. Conclusion： We can conclude that monitoring of serum marker CYFRA 21-1, early dudng first-line chemotherapy may be a useful prognostic tool for evaluation of early tumor response in patients with advanced NSCLC.

A New Chiral Synthesis of Naturally Occurring (-)-(S)-4-Hydroxy-2-pyrrolidinone

A new and convenient chiral synthesis of naturally occurring (-)-(S)-4-hydroxy-2-pyrrolidinone from (S)-malic acid was reported.

IONIC CONDUCTIVITY OF POLY (β-ALKOXYCARBONYLETHYLMETHYLSILOXANE)-LiClO_4 CROSSLINKED FILMS

Poly (β-carboxyethylmethylsiloxane)-LiClO_4 and poly (β-alkoxylethylmethylsiloxane)-LiClO_4 crosslinked fllms have been prepared. The ionic conductivity of the films depends on the polymer species, concentration of lithium perchlorate, temperature and content of crosslinking agent. The effect of high polar organic solvent 1, 4-butyrolactone on the ionic conductivity and mechanical properties of poly (β-carhoxyethylmethylsiloxane )-LiClO_4 system was also investignied.

MiR-16-5p plays an inhibitory role in human non-small cell lung cancer through Fermitin family member 2

Increasing evidence indicates that aberrant expressions of some microRNAs are associated with cancer progression.However,the roles and biological mechanisms of miRNA-16-5p in human non-small cell lung cancer(NSCLC)are not to be well studied.Here,we validated that the expression of miR-16-5p was decreased significantly in NSCLC samples and cell lines.The correlation between the clinicopathological features of NSCLC and the miR-16-5p expression showed that the expression of miR-16-5p in non-small cell lung cancer was linked with the advanced TNM stage,positive lymph node metastasis,with short overall survival(OS).Also,a negative correlation between miR-16-5p and Fermitin family member 2(FERMT2)was observed,implying there may be a potential link about their regulation.The hypothesis was further confirmed by in-silico analysis and dual-luciferase reporter assay.Moreover,we demonstrated that the transfections of miR-16-5p mimics could alter some biological characteristics of NSCLC cells remarkably accomplished by the expression variance of FERMT2 in vitro and in vivo assays.Summarily,this study demonstrated that miR-16-5p,as a tumor suppression factor in NSCLC by targeting FERMT2,could serve as one promising biomarker in the prediction for NSCLC patients.

Quaternary phosphonium polymer-supported dual-ionically bound[Rh(CO)I_(3)]^(2-)catalyst for heterogeneous ethanol carbonylation

A single-Rh-site catalyst(Rh-TPISP)that was ionically-embedded on a P(V)quaternary phosphonium porous polymer was evaluated for heterogeneous ethanol carbonylation.The[Rh(CO)I_(3)]^(2-)unit was proposed to be the active center of Rh-TPISP for the carbonylation reaction based on detailed Rh L3-edge X-ray absorption near edge structure(XANES),X-ray photoelectron spectroscopy(XPS),and Rh extended X-ray absorption fine structure(EXAFS)analyses.As the highlight of this study,Rh-TPISP displayed distinctly higher activity for heterogeneous ethanol carbonylation than the reported catalytic systems in which[Rh(CO)_(2)I_(2)]^(-)is the traditional active center.A TOF of 350 h^(-1)was obtained for the reaction over[Rh(CO)I_(3)]^(2-),with>95%propionyl selectivity at 3.5 MPa and 468 K.No deactivation was detected during a near 1000 h running test.The more electron-rich Rh center was thought to be crucial for explaining the superior activity and selectivity of Rh-TPISP,and the formation of two ionic bonds between[Rh(CO)I_(3)]^(2-)and the cationic P(V)framework([P]^(+))of the polymer was suggested to play a key role in firmly immobilizing the active species to prevent Rh leaching.

Preparation and Ionic Conductivity of Ultrafine Li_(2+x)RE_xSi_(1-x)O_3 by Sol-Gel Method

Li 2+ x RE x Si 1- x O 3(RE=Pr, Nd, Sm, Gd; x =0～0 15) samples were prepared by the sol gel method. DTA TG, XRD, TEM and A C impedance techniques were used to investigate the structure, shape, and ionic conductivity of these samples. The results show that the range of solid solution formation is in 0< x ≤0 09, in which the conductivity of these samples raises with the increasing amount of RE 2O 3 (RE=Pr, Nd, Sm, Gd) added, and the diameter of the powders is about 100 nm. Compared with the conventional solid state reaction, the sol gel method needs low temperature and presents high ionic conductivity.

Exact Traveling Wave Solutions of Nano-Ionic Solitons and Nano-Ionic Current of MTs Using the exp(-φ (ξ ))-Expansion Method

In this work, the exp(-φ (ξ )) -expansion method is used for the first time to investigate the exact traveling wave solutions involving parameters of nonlinear evolution equations. When these parameters are taken to be special values, the solitary wave solutions are derived from the exact traveling wave solutions. The validity and reliability of the method are tested by its applications to Nano-ionic solitons wave’s propagation along microtubules in living cells and Nano-ionic currents of MTs which play an important role in biology.