Relationship of egg parasitoids of rice planthoppers between rice and non-rice habitats

Egg parasitoids,Anagrus spp.and Oligositaspp.have been found to be important biologi-cal agents in suppressing rice planthoppers inAsian countries.However,most of these stud-ies have been confined to conditions within ricefields.In this study,field surveys were madeto study the floral and faunal diversity in vari-ous habitats surrounding rice fields.The riceand grass(Echinochloa spp.)plants were in-

Sustainable Management of Rice Insect Pests by Non-Chemical-Insecticide Technologies in China

Chemical pesticides play crucial roles in the management of crop diseases and pests. However, excessive and irrational use of pesticides has become a major concern and obstacle to sustainable agriculture. As a result, the quality and security of agricultural products are reduced, and the ecological and environmental integrities are threatened. Recently, environment-friendly pest management measures have been introduced and adopted to manage rice insect pests and reduce the use of insecticides. This paper reviewed the advancements in development and application of non-chemical technologies for insect pest management during rice production in China.

Growth Characteristics and Yield of Late-Season Rice under No-tillage and Non-flooded Cultivation with Straw Mulching

A long-term field experiment （started at 2003） was conducted to determine the effects of different dce cultivation methods on growth characteristics and grain yield of late-season rice under double-rice cropping system in seasonal drought region of southeast China （Yujiang County, Jiangxi Province）. The rice cultivation methods included no-tillage and flooded rice cultivation （N-F）, no-tillage and non-flooded rice cultivation with straw mulching （N-SM）, and no-tillage and non-flooded rice cultivation without straw mulching （N-ZM）. There was no significant difference in rice grain yield between the N-SM and N-F treatments. However, the rice grain yields in the N-SM and N-F treatments were significantly higher than that in the N-ZM treatment. The late-season rice plants in the N-SM treatment had significantly higher numbers of effective panicles and total grains per hill compared with those in the N-ZM treatment. The above-ground dry matter of late-season rice was similar between the N-SM and N-F treatments. Compared with the N-F treatment, the N-ZM and N-SM treatments significantly decreased the leaf area at the heading stage. Moreover, the N-SM treatment could significantly increase total root length and root tip number at the grain-filling stage compared with the N-ZM treatment.

Impact Assessments of Transgenic cry1Ab Rice on the Population Dynamics of Five Non-Target Thrips Species and Their General Predatory Flower Bug in Bt and Non-Bt Rice Fields Using Color Sticky Card Traps

A 2-yr field study was conducted to assess the effects of transgenic japonica rice（KMD1 and KMD2） with a synthetic cry1 Ab gene from Bacillus thuingiensis Berliner on population dynamics and seasonal average densities of five thrips species including Stenchaetothrips biformis（Bagnall）,Frankliniella intonsa（Trybom）,F.tenuicornis（Uzel）,Haplothrips aculeatus（Fabricius）,Haplothrips tritici（Kurd） and their general predatory flower bug,Orius similis Zheng as compared to the parental control rice line using the white,blue and yellow sticky card traps.Population dynamics and seasonal average densities of these five thrips species and their general predatory flower bug were not significantly affected by rice type.Additionally,the white sticky card trap was suggested to be the most suitable for monitoring the population of these five thrips species and their general predator.These results show that our tested Bt rice lines do not interrupt the population of non-target thrips species and their general predatory flower bug in the field,and also cannot result in more occurrence of these thrips species in the rice ecosystem.

Systematic Characterization of Long Non-Coding RNAs and Their Responses to Drought Stress in Dongxiang Wild Rice

Long non-coding RNAs(lncRNAs) play important roles in response to various biotic and abiotic stresses. So far, systematic identification and characterization of lncRNAs have been reported in a few model plant species and major crops, but their roles in abiotic stress response have not yet been reported in common wild rice(Oryza rufipogon). Dongxiang wild rice(DXWR) possesses a high degree of drought resistance and has been well recognized as a precious genetic resource for drought resistant rice breeding. We presented the reference catalog of 1 655 novel lncRNA transcripts in DXWR using strand-specific RNA sequencing and bioinformatics approaches. Meanwhile, a total of 1092 lncRNAs were determined as differentially expressed lncRNAs under drought stress. Quantitative real-time PCR results exhibited a high concordance with RNA sequencing data, which confirmed that the expression patterns of lncRNAs based on RNA sequencing were highly reliable. Furthermore, 8 711 transcripts were predicted as target genes of the differentially expressed lncRNAs. Functional annotation analysis based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases showed that the target genes were significantly enriched in cellular and metabolic processes, cell part, binding and plant hormone signal transduction, as well as many other terms related to abiotic stress resistance. These results expanded our understanding of lncRNA biology and provided candidate regulators for genetic improvement of drought resistance in rice cultivars.

Ecological Fitness of Non-vector Planthopper Sogatella furcifera on Rice Plants Infected with Rice Black Streaked Dwarf Virus

We evaluated the effects of rice black streak dwarf virus （RBSDV）-infested rice plants on the ecological parameters and its relevant defensive and detoxification enzymes of white-backed planthopper （WBPH） in laboratory for exploring the relationship between RBSDV and the non-vector planthopper. The results showed that nymph survival rate, female adult weight and fecundity, and egg hatchability of WBPH fed on RBSDV-infested rice plants did not markedly differ from those on healthy plants, whereas the female adult longevity and egg duration significantly shortened on diseased plants. Furthermore, significantly higher activities of defensive enzymes （dismutase, catalase and peroxidase） and detoxification enzymes （acetylcholinesterase, carboxylesterase and glutathione S-transferase） were found in WBPH adults fed on infected plants. Results implied that infestation by RBSDV increased the ecological fitness of non-vector planthopper population.

Effects of Panicle Nitrogen Fertilization on Non-Structural Carbohydrate and Grain Filling in Indica Rice

Grain filling, a crucial stage of grain yield formation in rice, is usually affected by the panicle nitrogen （N） fertilization. Field and pot culture experiments were conducted to explore the underlying mechanisms of N effect. Two rice cultivars with high lodging resistance were grown in the field and pot. Four panicle N fertilization treatments were conducted in 2006 and repeated in 2007. The result showed that medium level of panicle N fertilization treatment （NM） enhanced the accumulation and translocation of non-structural carbohydrate （NSC） in the stem and sheath. Compared with non-nitrogen treatment （NO）, NM promoted the translocation of labeled ^13C from stem and sheath to grain. But, low level of panicle N fertilization treatment （NL） and high level of panicle N fertilization treatment （NH） showed the negative effect. The endosperm cell, grain length, and grain width of NM increased more quickly than that of NO from 4 to 10 d after anthesis. During the early period of grain filling, sucrose-phosphate synthase （EC 2.4.1.14, SPS） activity were significantly higher for the NM treatment than those of the NL and NH treatments. Sucrose synthase （EC 2.4.1.13, SuSase） activity in the grains was substantially enhanced by NM, with the duration of higher activity being longer than those of the other treatments. At maturing stage, NM significantly increased the filled grain number, the seed-setting rate, and the grain weight compared with NL and NH. The results suggest that NM have a positive effect on the activities of enzymes of physiological importance, thereby increasing the grain size and promoting grain filling.

Effects of Non-flooded Cultivation with Straw Mulching on Rice Agronomic Traits and Water Use Efficiency

A field experiment was conducted to study water use efficiency and agronomic traits in rice cultivated in flooded soil and non-flooded soils with and without straw mulching. The total amount of water used by rice under flooded cultivation （FC） was 2.42 and 3.31 times as much as that by rice under the non-flooded cultivation with and without straw mulching, respectively. The average water seepage was 13 560 m^3/ha under the flooded cultivation, 4 750 m^3/ha under the non-flooded cultivation without straw mulching （ZM） and 4 680 m^3/ha under non-flooded cultivation with straw mulching （SM）. The evapotranspiration in the SM treatment was only 38.2% and 63.6% of the FC treatment and ZM treatment, respectively. Compared with the ZM treatment, straw mulching significantly increased leaf area per plant, main root length, gross root length and root dry weight per plant of rice. The highest grain yield under the SM treatment （6 747 kg/ha） was close to the rice cultivated in flooded soil （6 811.5 kg / ha）. However, the yield under the ZM treatment （4 716 kg/ha） was much lower than that under the FS treatment and SM treatment. The order of water use efficiency and irrigation water use efficiency were both as follows： SM〉 ZM〉 FC.

Progress on Transferring Elite Genes from Non-AA Genome Wild Rice into Oryza sativa through Interspecific Hybridization

The progress of research on transferring elite genes from non-AA genome wild rice into Oryza sativa through interspecific hybridization are in three respects, that is, breeding monosomic alien addition lines （MAALs）, constructing introgression lines （ILs） and analyzing the heredity of the characters and mapping the related genes. There are serious reproductive barriers, mainly incrossability and hybrid sterility, in the interspecific hybridization of O. sativa with non-AA genome wild rice. These are the ＇bottleneck＇ for transferring elite genes from wild rice to O. sativa. Combining traditional crossing method with biotechnique is a reliable way to overcome the reproductive barriers and to improve the utilizing efficiency of non-AA genome wild rice.

Characterization and Fine Mapping of Non-panicle Mutant (nop) in Rice

A mutant of panicle differentiation in rice called non-panicle （nop） was discovered in the progeny of a cross between 93-11 and Nipponbare. The mutant exhibits normal plant morphology but has apparently few tillers. The most striking change in nop is that its panicle differentiation is blocked, with masses of fluffy bract nodes generate from the positions where rachis branches normally develop in wild-type plants. Genetic analysis suggests that nop is controlled by a single recessive gene, which is temporarily named Nop（t）. Based on its mutant phenotype, Nop（t） represents a key gene controlling the initiation of inflorescence differentiation, By using simple sequence repeat markers and sequence tagged site markers, Nop（t） gene was fine mapped in a 102-kb interval on the long arm of chromosome 6. These results will facilitate the positional cloning and functional studies of the gene.

Optical Sum Frequency Generation Spectroscopy of Cracked Non-Glutinous Rice (Oryza sativa L.) Kernels

In order to study the correlation between the cracking of rice (Oryza sativa L.) kernels and the molecular structure of the amylopectin in them, we attempted optical sum frequency generation (SFG) spectroscopy in the C-H stretching vibration region for normal and cracked japonica non-glutinous rice kernels. The samples were Koshihikari and Nipponbare. In Nipponbare, the width of the SFG spectrum peak at 2915 cm- 1 of the cracked rice kernels was broader than that of the normal ones, while for Koshihikari there was no clear difference. The width of the 2915 cm- 1 peak is suggested to originate from the variety of the higher-order structure of the saccharide chains in amylopectin. Although this is a tentative result, this method is shown to have a potential of serving for preventing the cracking of the rice kernels.

Introgression of Gene for Non-Pollen Type Thermo-Sensitive Genic Male Sterility to Thai Rice Cultivars

For the two-line hybrid rice system, pol en sterility is regulated by recessive gene that responds to temperature. The recessive gene controlling thermo-sensitive genetic male sterility （TGMS） is expressed when the plants are grown in conditions with higher or lower critical temperatures. To transfer tgms gene（s） control ing TGMS to Thai rice cultivars by backcross breeding method, a male sterile line was used as a donor parent while Thai rice cultivars ChaiNat 1, PathumThani 1, and SuphanBuri 1 were used as recurrent parents. The BC2F2 lines were developed from backcrossing and selfing. Moreover, the simple sequence repeat （SSR） markers were developed for identifying tgms gene and the linked marker was used for assisting selection in backcrossing. The identification lines were confirmed by pol en observation. The results showed the success of introgression of the tgms gene into Thai rice cultivars. These lines will be tested for combining ability and used as female parent in hybrid rice production in Thailand.

基于AquaCrop模型的水稻多目标灌溉制度优化研究

【目的】优化现有水稻灌溉制度,节约灌溉用水量,减少稻田氮磷流失量以降低面源污染风险。【方法】构建了基于AquaCrop模型和NSGA-Ⅱ算法的水稻灌溉制度模拟优化模型,利用水稻田间试验数据,开展以产量最大、氮磷流失量最小、灌水次数最少的3种目标组合(产量-氮磷流失量(Y-TNP)、产量-灌水次数(Y-N)、产量-氮磷流失量-灌水次数(Y-TNP-N))下的灌溉制度优化模型研究,提出适应不同生育期降水年型的稳产-控污-提效灌溉制度。【结果】(1)与常规灌溉相比,Y-TNP灌溉制度优化水稻产量下降2.14%,氮磷流失量减少23.09%;Y-N灌溉制度优化水稻产量下降1.76%,灌水次数减少53%;Y-TNP-N灌溉制度优化水稻产量下降2.64%,氮磷流失量减少22.83%,灌水次数减少2次。(2)不同典型年以Y-TNP-N为目标优化的稳产-控污-提效灌溉制度水稻产量介于7.74~7.78 t/hm^(2),同时大幅度减少灌水量进而降低氮磷流失量。【结论】AquaCrop模型可模拟试验区水稻的生长发育过程,本文构建的模拟-优化耦合模型可用于优化不同生育期降水年型下稳产-控污-提效的灌溉制度。

取秧侧向零偏移的空间轨迹再生稻分插机构设计与试验

为了解决现有空间行星轮系式分插机构在再生稻宽窄行机械化种植取秧过程中出现侧向偏移量和侧向偏转角的技术难题,本文提出一种具有局部平面轨迹特性的空间轨迹不等速行星轮系机构,并开展基于取秧口和机构回转中心位置约束下的宽窄行分插机构综合研究。构建了基于关键位姿点(取秧起始点、取秧结束点、推秧点)的空间轮系机构运动综合模型,利用关键位姿点求解机构杆长参数与空间交错轴信息,并通过优选二杆相对角位移参数实现机构传动比分配。将不完全非圆齿轮副引入空间行星轮系机构,利用间歇机构锁止弧约束行星轴,实现机构取秧过程侧向零偏移量、侧向零偏转角的平面轨迹段。通过仿真分析与机构样机试验验证了机构实际作业性能与理论设计相一致,结果表明:分插机构取秧侧向零偏移量,取秧侧向零偏转角,推秧侧向总偏移量为50.24 mm、取秧角为5.18°、推秧角为71.56°、推秧侧向角为16.26°、插秧穴口宽度为22.43 mm、轨迹高度为289.76 mm,满足预期设计要求。最后通过田间试验验证,分插机构可实现等行距取秧口和既定机构回转中心下宽行(40 cm)与窄行(20 cm)间隔机插,满足再生稻宽窄行种植要求。

不同氮肥处理对香稻和非香稻品种籽粒灌浆和叶片光合特性的影响

在大田条件下,通过对香稻和非香稻品种的籽粒灌浆、叶片光合特性进行研究,明确香稻和非香稻品种产量和品质形成的生理机制。结果表明:在籽粒灌浆期,相对于非香稻品种,香稻品种在不施氮处理下表现为较高的籽粒灌浆速率,在施氮处理下表现为较低的籽粒灌浆速率;对于剑叶光合速率,无论在施氮还是非施氮条件下均表现为香稻品种高于非香稻品种,说明香稻品种更具有高光效特性。对2品种的产量性状分析表明,香稻品种较非香稻品种粒多,穗大;而非香稻品种相对于香稻品种则穗小,穗数多,这说明,香稻品种主要表现为大穗型,而非香稻品种主要表现为多穗型。对2品种的品质性状进行分析,加工品质2品种差异较小,外观品质表现为非香稻品种明显优于香稻品种,食味值2品种相差相对较小,且均表现为不施氮处理的食味值较高。综合以上分析表明,相对于非香稻品种,香稻品种在不施氮处理下主要表现为较高的籽粒灌浆速率,较高的剑叶光合速率,较高的产量及较高的食味品质;在施氮处理下,除光合速率香稻高于非香稻品种外,其他性状稍低于非香稻品种,这说明,在水稻提优栽培中,在确保产量不降低的情况下,不施或适当少施氮肥,有利于增加香稻品种的籽粒灌浆速率和剑叶光合速率,从而提高香稻的品质。

非圆齿轮-连杆组合式水稻钵苗膜上移栽机构优化设计与试验

为实现机械化一体式水稻钵苗膜上移栽要求,提出非圆齿轮-连杆组合式行星轮系移栽机构,用一套机构实现取苗、送苗、破膜挖穴和栽植4个工序协同配合作业,满足水稻钵苗膜上移栽所需的轨迹与姿态要求。对移栽机构进行理论分析并建立运动学模型,结合优化目标,基于Matlab GUI平台开发了数字可视化优化设计软件,通过人机交互获得1组满足移栽要求的机构参数。根据优化参数对机构开展结构设计与三维建模,通过ADAMS软件完成了虚拟样机仿真验证。研制了水稻钵苗膜上移栽机构物理样机与多功能试验测试台架,开展了移栽机构在空转和带苗状态下运动学特性和工作性能试验,结果表明,理论轨迹、虚拟样机仿真轨迹和物理样机台架试验轨迹在误差允许范围内保持一致,验证了移栽机构设计的一致性与正确性,性能试验结果:取苗成功率为94%,移栽成功率为92.36%,栽植株距变异系数为2.67%,满足移栽作业要求,验证了水稻钵苗膜上移栽机构设计的合理性与可行性。

水稻灌区面源污染治理研究进展

水稻生育期与雨热同期导致灌排机率高,也导致化肥中的氮磷伴随地表径流产生大量流失成为稻作区农业面源污染的主要来源。通过对水稻高效节水灌溉制度、农田退水污染迁移转化机理和灌区退水污染协同调控技术等方面国内外研究动态系统分析,并结合国家和学科发展需求,从退水污染机理解析、灌区水资源调配模拟、灌区面源水污协同调控等方面提出了稻作区面源污染治理研究中亟待解决的关键科学问题,以期为我国农业面源污染防控提供借鉴。

长江下游地区味优高产协同粳稻的品种特性

【目的】探明长江下游地区味优高产协同粳稻品种共性农艺与生理特征,为实现区域稻米产量和品质协同提升提供理论依据和技术支撑。【方法】于2018年和2022年,分别采用14个和13个常规粳稻品种开展品种筛选试验,系统比较不同品种的产量及构成、食味品质及质构特性,以及生物量、茎鞘非结构性碳水化合物(NSC)、群体光合势(LAD)等农艺与生理指标的差异。【结果】品种间的食味值和产量具有较大变幅,为此,按食味值和产量进行聚类,将不同水稻品种分为味中低产(ML)、味中高产(MH)和味优高产(GH)3种类型。GH类型品种2年的平均食味值和产量分别为68.5和10.2 t·hm^(-2),较MH和ML综合提高食味值6.8%、产量14.6%。从产量和品质性状的表现来看,与MH和ML相比,GH类型品种表现最高的结实率和口感得分。从生物量积累的过程来看,GH类型品种增加了灌浆初期的干物质积累比例,表现最高的抽穗-抽穗后15d的干物质积累。进一步分析此阶段干物质积累成因发现,GH类型品种在稳定增加LAD的同时,显著增加了茎鞘中NSC的转运率。相关性分析表明,水稻结实率与口感得分、抽穗-抽穗后15 d的干物质积累和LAD及NSC转运率均呈极显著正相关关系。【结论】在保持较高的抽穗-抽穗后15 d LAD(100.4 m^(2)·m^(-2)·d)的同时,进一步提高灌浆期茎鞘NSC的转运率(79.9%)促进灌浆启动,增加抽穗-抽穗后15 d干物质积累(3.6 t·hm^(-2)),提高水稻结实率(95.4%)和口感(9.6),是该区域味优高产协同水稻品种的共性特征。此外,研发以提高灌浆期NSC转运量和抽穗期植株茎鞘NSC积累为目标的水肥管理技术,有望进一步发挥上述味优高产协同品种的产量和食味品质潜力。

内源性物质对不同品种大米淀粉消化性的影响

近年来低血糖生成指数(GI)大米品种被培育出来,但是其产生低GI作用机理并不明确,内源性非淀粉物质作为大米关键成分,对不同GI大米淀粉消化率的影响值得探讨。本研究以典型的2种不同GI大米作为研究对象,探究了去除内源性非淀粉物质对大米淀粉体外消化性、理化性质和微观结构的影响。结果表明,对于低GI品种,蛋白质和膳食纤维的去除显著促进了淀粉消化,使最终消化率提升了7.37%~13.28%,但高GI品种的内源性非淀粉物质对于淀粉消化性的影响并不显著。在理化性质上,蛋白质和膳食纤维的去除将淀粉的膨胀力显著提升至(17.83±0.30)~(13.44±0.27)g/g,显著改变了米粉的糊化特性和热力学特性,从而使其更容易糊化和消化。在微观结构上,去除蛋白质和膳食纤维使淀粉颗粒结构减小了3~8μm,蛋白质、膳食纤维和淀粉的相互作用提高了米粉的有序度和聚集结构。