Comparison of the clinical characteristics and survival between Uyghur patients with hepatitis virus-related and non-B, non-C hepatocellular carcinoma in Xinjiang, China

Objective： To compare the clinical characteristics and prognosis between hepatitis virus-related hepatocellular carcinoma（viral HCC） and non-B, non-C HCC（NBC-HCC） among Uyghur patients in Xinjiang province, China. Methods： Between 01/01/2000 and 31/12/2012, 319 Uyghur HCC patients were treated at the Cancer Centre of The First Affiliated Hospital of Xinjiang Medical University. The data for the patients were obtained from a retrospective review of the patients＇ medical records. A total of 18 patients were excluded from the study because of incomplete information. The patients were classified into two groups： viral HCC and NBC-HCC. The clinical characteristics and prognostic factors were statistically analysed.Results： For all 301 patients, gender（P=0.000）, area of residence（P=0.002）, diabetes mellitus（P=0.009）, BMI（P=0.000）, cirrhosis（P=0.000）, tumour stage（P=0.004）, Child-Pugh class（P=0.000）, the TBIL level（P=0.000）, and the alpha-fetoprotein（AFP） level（P=0.000） were significantly different between the NBC-HCC and viral HCC groups. The NBC-HCC patients tended to be diagnosed at advanced stages; however, the NBC-HCC patients exhibited lower Child-Pugh scores than the viral HCC patients. In all patients examined, the 0.5-, 1-, 3- and 5-year overall survival（OS） rates were 35.6%, 20.3%, 12.6% and 4.5%, respectively. No significant difference in OS was observed between the two groups（P=0.124）. Cox multivariate analysis revealed that age（RR =1.539, P=0.001）, TNM stage（RR =12.708, P=0.000）, portal vein tumour thrombus（PVTT）（RR =2.003, P=0.000）, Child-Pugh class（RR =1.715, P=0.000）, and TACE ＋ radiotherapy/RFA（RR =0.567, P=0.000） were significant independent prognostic factors for HCC patients. Conclusions： The clinical characteristics differ between Uyghur patients with NBC-HCC and viral HCC. HCC in the Xinjiang region displays specific regional characteristics. Age, TNM stage, PVTT, Child-Pugh class and TACE ＋ radiotherapy/RFA are significant risk factors that influence patient survival.

A mutation of the start codon in the X region of hepatitis B virus DNA in a patient with non-B,non-C chronic hepatitis

There are cases of hepatitis involving occult hepatitis B virus(HBV)infection in which,even though the HB surface antigen(HBsAg)is negative,HBV-DNA is detected by a polymerase chain reaction(PCR).We con-ducted a sequence analysis of the entire HBV region in a case of non-B non-C chronic hepatitis in a 46-yearold female.A diagnosis of non-B non-C chronic hepatitis was made.Although HBV markers,such as HBs antibody(anti-HBs),anti-HBc,HBeAg and anti-HBe,were negative,HBV-DNA was positive.Nested PCR was performed to amplify the precore region of HBV-DNA and all remaining regions by long nested PCR.Sequence analysis of the two obtained bands was conducted by direct sequencing.Compared with the control strains,the ATG(Methionine)start codon in the X region had mut ated to GTG(Valine).It is assumed that a mutation at the start codon in the X region may be the reason why HBV markers are negative in some cases of hepatitis that involve occult HBV infection.

Occult hepatitis B virus infection and surgical outcomes in non-B, non-C patients with curative resection for hepatocellular carcinoma

AIM To investigate the prevalence, clinicopathological characteristics and surgical outcomes of occult hepatitis B virus(HBV) infection(OBI) in patients with non-B, non-C(NBNC) hepatocellular carcinoma(HCC).METHODS This study retrospectively examined the cases of 78 NBNC patients with curative resection for HCC for whom DNA could be extracted from formalin-fixed paraffin-embedded tissue. OBI was determined by the HBV-DNA amplification of at least two different sets of primers by TaqM an realtime polymerase chain reaction. Possibly carcinogenetic factors such as alcohol abuse, diabetes mellitus, obesity and non-alcoholic steatohepatitis(NASH) were examined. Surgical outcomes were evaluated according to diseasefree survival(DFS), overall survival(OS) and diseasespecific survival(DSS).RESULTS OBI was found in 27/78 patients(34.6%) with NBNC HCC. The OBI patients were significantly younger than the non-OBI cases at the time of surgery(average age 63.0 vs 68.1, P = 0.0334) and the OBI cases overlapped with other etiologies significantly more frequently compared to the non-OBI cases(P = 0.0057). OBI had no impact on the DFS, OS or DSS. Only tumorrelated factors affected these surgical outcomes.CONCLUSION Our findings indicate that OBI had no impact on surgical outcomes. The surgical outcomes of NBNC HCC depend on early tumor detection; this reconfirms the importance of a periodic medical examination for individuals who have NBNC HCC risk factors.

Full-State-Constrained Non-Certainty-Equivalent Adaptive Control for Satellite Swarm Subject to Input Fault

Satellite swarm coordinated flight(SSCF)technology has promising applications,but its complex nature poses significant challenges for control implementation.In response,this paper proposes an easily solvable adaptive control scheme to achieve high-performance trajectory tracking of the SSCF system subject to actuator efficiency losses and external disturbances.Most existing adaptive controllers based on the certaintyequivalent(CE)principle show unpredictability and nonconvergence in their online parameter estimations.To overcome the above vulnerabilities and the difficulties caused by input failures of SSCF,this paper proposes an adaptive estimator based on scaling immersion and invariance(I&I),which reduces the computational complexity while improving the performance of the parameter estimator.Besides,a barrier Lyapunov function(BLF)is applied to satisfy both the boundedness of the system states and the singularity avoidance of the computation.It is proved that the estimator error becomes sufficiently small to converge to a specified attractive invariant manifold and the closed-loop SSCF system can obtain asymptotic stability under full-state constraints.Finally,numerical simulations are performed for comparison and analysis to verify the effectiveness and superiority of the proposed method.

Impact of smoking habit on surgical outcomes in non-B non-C patients with curative resection for hepatocellular carcinoma

AIM To analyzed the correlation between smoking status and surgical outcomes in patients with non-B non-C hepatocellular carcinoma(NBNC-HCC), and we investigated the patients' clinicopathological characteristics according to smoking status.METHODS We retrospectively analyzed the consecutive cases of 83 NBNC-HCC patients who underwent curative surgical treatment for the primary lesion at Saga University Hospital between 1984 and December 2012. We collected information about possibly carcinogenic factors such as alcohol abuse, diabetes mellitus, obesity and smoking habit from medical records. Smoking habits were subcategorized as never, ex- and current smoker at the time of surgery. The diagnosis of nonalcoholic steatohepatitis(NASH) was based on both clinical information and pathological confirmation.RESULTS Alcohol abuse, diabetes mellitus, obesity and NASH had no significant effect on the surgical outcomes. Current smoking status was strongly correlated with both overall survival(P = 0.0058) and disease-specific survival(P = 0.0105) by multivariate analyses. Subset analyses revealed that the current smokers were significantly younger at the time of surgery(P = 0.0002) and more likely to abuse alcohol(P = 0.0188) and to have multiple tumors(P = 0.023).CONCLUSION Current smoking habit at the time of surgical treatment is a risk factor for poor long-term survival in NBNC-HCC patients. Current smokers tend to have multiple HCCs at a younger age than other patients.

Non-coding RNAs in hepatitis C-induced hepatocellular carcinoma:Dysregulation and implications for early detection,diagnosis and therapy

Hepatitis C virus(HCV)infection is one of main causes of hepatocellular carcinoma(HCC)and the prevalence of HCV-associated HCC is on the rise worldwide.It is particularly important and helpful to identify potential markers for screening and early diagnosis of HCC among high-risk individuals with chronic hepatitis C,and to identify target molecules for the prevention and treatment of HCV-associated-HCC.Small noncoding RNAs,mainly microRNAs(miRNAs),and long non-coding RNAs(lncRNAs)with size greater than 200nucleotides,are likely to play important roles in a variety of biological processes,including development and progression of HCC.For the most part their underlying mechanisms of action remain largely unknown.In recent years,with the advance of high-resolution of microarray and application of next generation sequencing techniques,a significant number of non-coding RNAs(ncRNAs)associated with HCC,particularly caused by HCV infection,have been found to be differentially expressed and to be involved in pathogenesis of HCVassociated HCC.In this review,we focus on recent studies of ncRNAs,especially miRNAs and lncRNAs related to HCV-induced HCC.We summarize those ncRNAs aberrantly expressed in HCV-associated HCC and highlight the potential uses of ncRNAs in early detection,diagnosis and therapy of HCV-associated HCC.We also discuss the limitations of recent studies,and suggest future directions for research in the field.miRNAs,lncRNAs and their target genes may represent new candidate molecules for the prevention,diagnosis and treatment of HCC in patients with HCV infection.Studies of the potential uses of miRNAs and lncRNAs as diagnostic tools or therapies are still in their infancy.

Hepatocellular carcinoma in non-cirrhotic liver: A comprehensive review

Hepatocellular carcinoma(HCC) is the most common type of primary liver cancer, which in turns accounts for the sixth most common cancer worldwide.Despite being the 6 th most common cancer it is the second leading cause of cancer related deaths. HCC typically arises in the background of cirrhosis, however,about 20% of cases can develop in a non-cirrhotic liver. This particular subgroup of HCC generally presents at an advanced stage as surveillance is not performed in a non-cirrhotic liver. HCC in non-cirrhotic patients is clinically silent in its early stages because of lack of symptoms and surveillance imaging; and higher hepatic reserve in this population. Interestingly, F3 fibrosis in non-alcoholic fatty liver disease, hepatitis B virus and hepatitis C virus infections are associated with high risk of developing HCC. Even though considerable progress has been made in the management of this entity, there is a dire need for implementation of surveillance strategies in the patient population at risk, to decrease the disease burden at presentation and improve the prognosis of these patients. This comprehensive review details the epidemiology, risk factors, clinical features,diagnosis and management of HCC in non-cirrhotic patients and provides future directions for research.

C反应蛋白联合全身免疫炎症指数对非老年动脉瘤性蛛网膜下腔出血的预后价值

目的探讨C反应蛋白(CRP)、全身免疫炎症指数(SⅡ)对非老年动脉瘤性蛛网膜下腔出血(aSAH)患者的预后价值。方法收集2021年7月至2023年4月郑州大学第五附属医院住院的108例aSAH患者的临床资料,根据出院3个月的改良Rankin量表(mRS)评分标准将患者分为预后良好组(mRS 0~2分)和预后不良组(mRS 3~6分),比较两组患者的临床资料,分析预后不良的独立影响因素,并绘制受试者工作特征(ROC)曲线评估不同独立影响因素对患者预后不良的预测价值。结果108例非老年aSAH患者中预后不良组48例,预后良好组60例。单因素分析结果显示,两组患者在Fisher分级、Hunt-Hess分级、CRP、白细胞计数、淋巴细胞计数、中性粒细胞计数、SⅡ方面差异均有统计学意义(P<0.05)。多因素logistic回归分析结果显示,Fisher分级、CRP、SⅡ是非老年aSAH患者预后不良的独立影响因素。ROC曲线分析结果显示CRP与SⅡ预测非老年aSAH患者预后不良的敏感度、特异度、曲线下面积(AUC)分别为99.9%、60.0%、0.874和95.8%、63.3%、0.881,二者联合预测非老年aSAH患者预后不良的敏感度、特异度、AUC分别为72.9%、95.0%、0.911。结论升高的CRP与SⅡ是非老年aSAH患者预后不良的重要指标,二者联合的预测价值最高。

非高密度脂蛋白胆固醇对绝经后女性心血管疾病的影响

目的:探讨非高密度脂蛋白胆固醇(non-HDL-C)水平对绝经后女性心血管疾病(CVD)的影响。方法:采用前瞻性队列研究方法,选取2006~2018年参加开滦集团职工健康体检中总胆固醇、高密度脂蛋白胆固醇资料完整且无CVD病史的8893例已绝经女性为研究对象,以发生CVD、死亡或2021年12月31日随访结束为随访终点。根据《中国血脂管理指南(2023年)》,将研究对象分为non-HDL-C<4.1 mmol/L组(n=6079)、4.1 mmol/L≤non-HDL-C<4.9 mmol/L组(n=1824)和non-HDL-C≥4.9 mmol/L组(n=990)。用Kaplan-Meier法计算不同non-HDL-C水平分组的CVD累积发病率,并以log-rank进行检验。采用多因素Cox回归模型分析不同non-HDL-C水平对CVD的影响。结果:平均随访(10.78±4.48)年期间,non-HDL-C<4.1 mmol/L组、4.1 mmol/L≤non-HDL-C<4.9 mmol/L组和non-HDL-C≥4.9 mmol/L组的CVD累积发病率分别为1.82%、3.24%、2.89%,Kaplan-Meier生存曲线分析显示,三组累积发病率差异有统计学意义(log-rank P<0.0001)。Cox回归分析结果显示,校正年龄、性别等混杂因素后,与non-HDL-C<4.1 mmol/L组相比,4.1 mmol/L≤non-HDL-C<4.9 mmol/L组与non-HDL-C≥4.9 mmol/L组发生CVD的HR(95%CI)值分别为1.40(1.13~1.74)、1.35(1.03~1.78)。结论:高non-HDL-C水平是绝经后女性发生CVD的独立危险因素。

商业载人航天器测控通信模式初步构想

随着商业航天的快速发展与可重复使用等前沿技术应用的逐步成熟,以往以国家行为为主体的载人航天领域已开始积极吸纳商业航天力量融合发展。针对载人航天高可靠性要求与商业航天低成本控制之间的矛盾,梳理分析了现役载人航天器测控需求、链路配置与使用模式,在此基础上,综合考虑载人航天传统需求与商业航天特点,提出了标配+选配的测控链路配置模式;针对在轨关键事件与常态化运行场景,设计了中继、对地等传统链路与卫星互联网系统、北斗短报文系统等非传统链路相结合,支持遥测、遥控、图像、话音等多样化业务的载人航天器测控通信模式。相关研究成果可为商业载人航天器测控通信设计提供借鉴参考。

UVC技术灭活常见病原微生物研究现状及临床应用

作为一种新型非抗生素治疗方法,波长范围为100~400 nm的紫外线已被证明具有一定的抗菌特性。不同波长紫外线的灭活效果存在差异,生物安全性也不尽相同。近年在这个领域涌现一批研究报道,尤其是波长范围为200~280 nm的C波段紫外线(Ultraviolet C,UVC)成为研究热点。本文对这一波长范围的研究新发现进行了综述,包括UVC灭活不同病原微生物的作用机制、影响因素及灭活效果,分析UVC技术的生物安全性,并简要归纳UVC技术设备在临床应用的研究进展,以期为相关研究提供参考。

浮针治疗急性非特异性腰背痛临床研究

目的研究浮针治疗急性非特异性腰背痛的临床疗效、慢性转归率和安全性。方法选取2020年1月—2023年6月深圳平乐骨伤科医院(深圳市坪山区中医院)收治的急性非特异性腰背痛患者256例,根据患者治疗方式不同分为浮针组和对照组,浮针组129例,对照组127例。两组患者均予中药封包治疗,对照组加服洛芬待因缓释片、盐酸乙哌立松片,浮针组在上述治疗基础上再加用浮针治疗,比较两组患者临床疗效、复发率、腰部疼痛[视觉疼痛模拟分法(VAS)评分]、腰部功能障碍[腰椎功能障碍指数评分量表(ODI)评分]、指地间距(FFD)。结果治疗前两组患者VAS评分、ODI评分、FFD比较,差异无统计学意义(P>0.05);治疗后,两组患者VAS评分、ODI评分、FFD较治疗前显著下降,且浮针组VAS评分、ODI评分、FFD均低于对照组,差异有统计学意义(P<0.05)。浮针组复发率低于对照组,临床疗效高于对照组,差异有统计学意义(P<0.05)。结论浮针治疗急性非特异性腰背痛能够快速高效地降低痛感、改善腰背功能,降低复发率,近期疗效和远期疗效较好,值得临床应用。

鼻咽癌组织中LncRNA CTBP1-AS2和miR-140-5p水平表达与放疗疗效及预后的关系研究

目的探讨鼻咽癌癌组织中长链非编码RNA羧基末端结合蛋白1反义RNA2(long non-coding RNA C-terminal binding protein 1 antisense RNA2,LncRNA CTBP1-AS2)、微小核糖核酸(microRNA,miR)-140-5p水平表达与放疗疗效及预后的关系。方法选取2018年3月~2020年3月于南通市肿瘤医院确诊的222例鼻咽癌患者为鼻咽癌组,记录患者临床资料,评估放疗疗效及预后,并分为生存组(n=194)和死亡组(n=28);另选取同期219例鼻咽炎患者为对照组。采用Pearson相关分析检验鼻咽癌患者中LncRNA CTBP1-AS2与miR-140-5p表达水平的相关性;采用Kaplan-Meier生存曲线分析鼻咽癌组织中LncRNA CTBP1-AS2,miR-140-5p表达水平与患者预后的关系;采用COX比例风险回归模型对鼻咽癌患者预后进行多因素分析。结果鼻咽癌组患者组织中LncRNA CTBP1-AS2表达水平(2.25±0.46)高于对照组(1.02±0.22),miR-140-5p表达水平(0.67±0.19)低于对照组(1.01±0.23),差异具有统计学意义(t=35.742,16.934,均P<0.001)。鼻咽癌患者中LncRNA CTBP1-AS2与miR-140-5p表达水平呈负相关(r=-0.624,P<0.001)。LncRNA CTBP1-AS2高表达的鼻咽癌患者放疗后总有效率(74.11%)和三年生存率(77.68%)低于低表达患者(93.64%,97.27%),差异具有统计学意义(χ^(2)=15.578,19.331,均P<0.001);miR-140-5p高表达患者放疗后总有效率(93.58%)和三年生存率(96.33%)显著高于低表达患者(74.34%,78.76%),差异具有统计学意义(χ^(2)=15.119,15.538,均P<0.001)。死亡组磁共振酰胺质子转移(amide proton transfer,APT)值(2.10±0.26)、放疗无效(85.71%)、LncRNA CTBP1-AS2高表达(89.29%)及miR-140-5p低表达(85.71%)患者比例高于生存组(1.82±0.31,6.19%,44.85%,45.88%),差异具有统计学意义(t/χ^(2)=4.551,108.127,19.331,15.538,均P<0.001)。LncRNA CTBP1-AS2表达水平为鼻咽癌患者三年内死亡的危险因素(HR=2.762,95%CI:1.510~5.051,P=0.001),miR-140-5p表达水平为鼻咽癌患者三年内死亡的保护因素(HR=0.817,95%CI:0.718~0.930,P=0.002)。结论鼻咽癌组织中LncRNA CTBP1-AS2高表达,miR-140-5p低表达,二者与放疗疗效及预后关系密切。

基于转录组测序探究C-MET表达在非小细胞肺癌中的免疫调控机制

目的通过转录组测序技术分析C-MET表达在非小细胞肺癌中的免疫调控机制。方法使用siRNA分子干扰技术将C-MET高表达肺腺癌细胞株(H1993)、肺鳞癌细胞株(EBC-1)的C-MET表达沉默,利用转录组测序技术检测C-MET沉默前后细胞差异表达的基因(DEGs),通过生物信息学分析挖掘出C-MET可能参与调控的免疫微环境信号通路及相关基因。最后使用人免疫细胞与H1993、EBC-1共培养技术验证C-MET对免疫因子(INF-γ、INF-β、CXCL-10)的影响。结果通过转录组测序技术共检测到505个DEGs,其中H1993的C-MET调控前后表达差异组的表达差异基因共有38个,上调的差异表达基因有24个,下调的差异表达基因有14个。EBC-1的C-MET调控前后表达差异组的表达差异基因共有467个,上调的差异表达基因有347个,下调的差异表达基因121个。差异基因的KEGG分析表明,C-MET表达可能通过白细胞介素(IL-17)信号通路、白细胞分化、细胞因子受体活性、细胞周期、细胞因子-细胞因子受体相互作用参与免疫细胞调节因子的调控。使用肺癌细胞与人免疫细胞共同培养技术验证C-MET对免疫因子分泌的影响,Rt-qPCR检测结果提示:与C-MET高表达组共培养的PBMC中干扰素(INF-γ)的m RNA转录水平是低表达组的77倍、CXCL-10的mRNA转录水平是低表达组的1.6倍,INF-β的mRNA转录水平是低表达组的2倍。结论C-MET表达可能通过IL-17信号通路、白细胞分化、细胞因子受体活性通路参与肿瘤周围免疫微环境调控。

ACAT1和MTNR1B基因多态性与非酒精性脂肪性肝病易感性的关系

目的本研究拟探讨乙酰辅酶A乙酰转移酶1(ACAT1)和褪黑激素受体1B(MTNR1B)基因多态性与非酒精性脂肪性肝病(NAFLD)疾病易感性的关系。方法本研究共纳入2020年12月—2022年6月就诊于青岛市市立医院的健康体检者164例、NAFLD患者228例。采用PCR及测序的方法对ACAT1 rs1044925、rs1157651和MTNR1B rs10830963基因多态性进行基因分型,并采集空腹静脉血进行生化检测。符合正态分布的计量资料两组间比较采用成组t检验;非正态分布的计量资料两组间比较采用Mann-Whitney U非参数检验;计数资料两组间比较采用χ2检验。结果ACAT1 rs1044925、rs1157651和MTNR1B rs10830963基因型分布在NAFLD及健康对照组间无统计学差异(P值均>0.05),ACAT1 rs1044925 AA基因型携带者的LDL水平明显高于C等位基因携带者(Z=−2.08,P=0.04),MTNR1B rs10830963 G等位基因携带者空腹血糖水平明显高于CC基因型携带者(Z=−3.01,P<0.01)。结论ACAT1 rs1044925、rs1157651和MTNR1B rs10830963多态性与NAFLD易感性无明显相关,ACAT1 rs1044925和MTNR1B rs10830963位点分别与LDL和空腹血糖水平有关。

C-V2X网联环境下应急优先的绿灯补偿模型研究

针对在路口实施应急车辆优先策略会对非优先相位交通造成影响的问题,在蜂窝车联网(C-V2X)环境下进行了均衡非优先相位通行效益的绿灯补偿模型研究。搭建了相应的算法模型,并对一辆应急车辆的场景进行了模拟。该模型在不影响应急车辆优先通行的前提下,根据非优先相位实时交通需求计算补偿绿灯时间,从而最小化应急优先对非优先相位的车辆通行影响。仿真结果证明,所提模型在不同的饱和度状态下均可有效地降低应急车辆通过后非优先相位车辆的平均延误时间和平均排队长度。所提模型对于应急车辆通过红绿灯路口的策略进行了优化,提高了通行效率。

慢性丙型肝炎合并非酒精性脂肪肝患者血清25(OH)D水平变化及其与HCV-RNA载量、糖脂代谢的关系

目的 探讨慢性丙型肝炎(CHC)合并非酒精性脂肪性肝病(NAFLD)患者血清25-羟维生素D[25(OH)D]水平及其与HCV-RNA载量、糖脂代谢的关系。方法 选取2018年9月至2021年9月医院收治CHC患者174例,根据是否合并NAFLD分为单纯CHC组(110例)和CHC-NAFLD组(64例),选取同期体检健康成人87例(健康组),使用酶联免疫吸附法检测纳入对象血清25(OH)D水平,生化分析检测糖脂代谢指标,荧光定量PCR法检测CHC患者HCV-RNA载量,spearman相关性分析血清25(OH)D水平与HCV-RNA载量、糖脂代谢指标水平的相关性,logistics回归分析CHC合并NAFLD的危险因素。结果 CHC-NAFLD组、CHC组患者中BMI≥28kg/m^(2)比率、HbA1c、FBG、INS、IR、TG和LDL-C水平显著高于健康组(P<0.05),血清25(OH)D水平显著低于健康组(P<0.05)。CHC-NAFLD组BMI≥28kg/m^(2)比率、HbA1c、FBG、INS、IR、TG、LDL-C水平和HCV-RNA载量显著高于CHC组(P<0.05),血清25(OH)D水平显著低于CHC组(P<0.05)。25(OH)D水平与HbA1c、FBG、IR、TG水平呈负相关(r=-0.426、-0.387、-0.406、-0.514,P<0.001)。25(OH)D<42 nmol/L、BMI≥28 kg/m^(2)、TG≥1.72 mmol/L、HCV-RNA载量≥3.5×10^(6) IU/mL是CHC患者合并NAFLD的危险因素(P<0.05)。结论 CHC合并NAFLD患者血清25(OH)D水平降低,25(OH)D水平与HbA1c、FBG、IR、TG呈负相关且其水平升高会增加CHC患者发NAFLD发生风险。

结合非局部空间信息和KL信息的鲁棒FCM算法

针对传统模糊C均值(Fuzzy C-Means,FCM)聚类算法对噪声敏感的问题,提出一种结合非局部空间信息和KL信息的鲁棒FCM算法.首先,将灰度信息与非局部空间信息相融合,用于增强算法对噪声的鲁棒性;其次,在目标函数中引入KL信息,以便减少分割的模糊性.在密度为5%的混合噪声条件下,合成图像和自然图像的实验结果表明,该文算法的分割精度较高、鲁棒性较强,能较好地分割噪声图像.

冠心病患者血清胱抑素C、高敏C反应蛋白、非高密度脂蛋白胆固醇与血流储备分数相关性研究

目的:探讨冠心病(CHD)患者血清胱抑素C(CysC)、高敏C反应蛋白(hs-CRP)、非高密度脂蛋白胆固醇(non-HDL-C)与血流储备分数的相关性。方法:选取150例冠心病患者为研究对象,行冠状动脉造影(CAG)检查,以血流储备分数(FFR)为分组标准,FFR>0.75组81例、FFR≤0.75组69例,比较两组患者冠脉造影结果,血清CysC、hs-CRP、non-HDL-C水平,分析血清CysC、hs-CRP、non-HDL-C与FFR值的相关性及FFR值影响因素。结果:FFR≤0.75组管腔狭窄比高于FFR>0.75组及最小管腔直径小于FFR>0.75组(均P<0.05)。FFR≤0.75组血清CysC、hs-CRP、non-HDL-C水平高于FFR>0.75组(均P<0.05)。血清CysC、hs-CRP、non-HDL-C水平与冠心病患者FFR值均呈负相关(均P<0.05)。Gensini总评分、纤维蛋白原(FG)、CysC、hs-CRP、non-HDL-C为影响CHD患者FFR值的危险因素(均P<0.05)。结论:冠心病患者FFR值降低与血清CysC、hs-CRP、non-HDL-C水平密切相关,可为临床初步判断CHD患者是否存在心肌缺血提供依据。

高州油茶果实膨大期光合产物的运输和分配特征

【目的】研究高州油茶在果实快速膨大期光合产物的运输和分配特征,进一步掌握南方特色油茶品种果实品质与光合产物分配的相关性,为华南地区油茶产业高质量发展提供理论支撑。【方法】采用^(13)C同位素脉冲标记法,对高州油茶饲喂^(13)CO_(2)气体2 h,在标记后0、6、24、48、72 h分别测定叶、枝、果皮和种仁的^(13)C含量,同时测定其叶片、种仁和果皮中的糖组分特征和非结构性碳水化合物(NSC)。【结果】1)^(13)C光合产物在叶中合成后,在标记结束后0~6 h向各库器官的运输速率最大,6~48 h光合产物运输趋于稳定。2)光合产物运输趋于稳定后,在各器官的分配比从大到小依次为叶>种仁>果皮>枝。高州油茶光合作用固定的^(13)C光合产物在标记结束后0~72 h消耗了95.07%。3)光合产物主要以果糖的形式在各器官存储,叶与果实之间存在着较高的蔗糖质量浓度梯度。4)各器官非结构性碳水化合物(NSC)积累量为果皮>叶>种仁。可溶性糖/淀粉比值为种仁最大,果皮次之,叶最小。【结论】果实膨大期高州油茶叶中合成的光合产物在合成后的24 h内向各库器官快速运输,大多光合产物被植物呼吸消耗。近期合成的光合产物在各器官中分配比以叶最高,不利于高州油茶果实发育。叶与果皮中较高的蔗糖质量浓度是高州油茶果皮较厚的原因之一。果实快速膨大期果皮发育、脂肪酸合成和油脂积累是种仁与果皮中可溶性糖/淀粉比值较高的原因。