Non-O157 STEC has been shown to have a diverse ecological distribution among food-animals. It has been associated with both outbreaks and individual cases of severe illness. This group of the organisms is now consider...Non-O157 STEC has been shown to have a diverse ecological distribution among food-animals. It has been associated with both outbreaks and individual cases of severe illness. This group of the organisms is now considered as a major contributor to human disease. The clinical description of the diseases caused by these organisms is reviewed. The host specificity of these pathogens is described and discussed. These organisms appear widespread among food animals like cattle and sheep, and can therefore affect a range of foods directly from the meat and excretions of these animals being used in farming practices. This article reviews the origins, diversity and pathogenesis of non-O157 STEC.展开更多
Objective:The present study has characterized dialyzed toxin from non-0157 VTEC E.coli isolates by vero cell toxicity assay and pathogenecity in mice model.Methods:Toxins from non-0157 verocytotoxic Escherichia coli i...Objective:The present study has characterized dialyzed toxin from non-0157 VTEC E.coli isolates by vero cell toxicity assay and pathogenecity in mice model.Methods:Toxins from non-0157 verocytotoxic Escherichia coli isolated from neonatal calves were characterized.Dialyzed toxin from E.coli 026,0111 and 0103 serotypes were prepared and characterized by verocell toxicity assay and pathogenicity in mice model.E.coli 0157:H7 considered as positive control for this study.Results:Cytopathic effects in vero cell line first rounding of vero cells,followed by clumping of cells and finally disintegrated,blackened,shriveled cell line within 16 to 72 hrs.Phenotypic markers such as hind limb paralysis and reddening of tail were prominent in all the toxicated mice.Extensive histopathological study was conducted for multiple organ involvement.Conclusion: Several methods for toxin assay were developed based on biological,immunological and detection of virulence genes related to toxin production but each test has draw back.Therefore,it is likely that future effort will be focused on the development of assay,which is fast,reliable,specific and sensitive methods based on mice model.展开更多
RECOMBINANT GLYCOPROTEINS AS NOVEL ANTIGENIC TARGETS FOR DIAGNOSIS OF HEMOLYTIC UREMIC SYNDROME Human infection with Shiga toxin-producing Escherichia coli(STEC)is a major cause of postdiarrheal hemolytic uremic syndr...RECOMBINANT GLYCOPROTEINS AS NOVEL ANTIGENIC TARGETS FOR DIAGNOSIS OF HEMOLYTIC UREMIC SYNDROME Human infection with Shiga toxin-producing Escherichia coli(STEC)is a major cause of postdiarrheal hemolytic uremic syndrome(HUS),a life-threatening condition characterized by hemolytic anemia,thrombocytopenia and acute renal failure.E.coli O157:H7 is the dominant STEC serotype associated with HUS worldwide although non-O157 STEC serogroups can cause a similar disease.Detection of anti-O157 LPS antibodies in combination with bacteriological procedures considerably improves diagnosis of STEC infections.展开更多
文摘Non-O157 STEC has been shown to have a diverse ecological distribution among food-animals. It has been associated with both outbreaks and individual cases of severe illness. This group of the organisms is now considered as a major contributor to human disease. The clinical description of the diseases caused by these organisms is reviewed. The host specificity of these pathogens is described and discussed. These organisms appear widespread among food animals like cattle and sheep, and can therefore affect a range of foods directly from the meat and excretions of these animals being used in farming practices. This article reviews the origins, diversity and pathogenesis of non-O157 STEC.
文摘Objective:The present study has characterized dialyzed toxin from non-0157 VTEC E.coli isolates by vero cell toxicity assay and pathogenecity in mice model.Methods:Toxins from non-0157 verocytotoxic Escherichia coli isolated from neonatal calves were characterized.Dialyzed toxin from E.coli 026,0111 and 0103 serotypes were prepared and characterized by verocell toxicity assay and pathogenicity in mice model.E.coli 0157:H7 considered as positive control for this study.Results:Cytopathic effects in vero cell line first rounding of vero cells,followed by clumping of cells and finally disintegrated,blackened,shriveled cell line within 16 to 72 hrs.Phenotypic markers such as hind limb paralysis and reddening of tail were prominent in all the toxicated mice.Extensive histopathological study was conducted for multiple organ involvement.Conclusion: Several methods for toxin assay were developed based on biological,immunological and detection of virulence genes related to toxin production but each test has draw back.Therefore,it is likely that future effort will be focused on the development of assay,which is fast,reliable,specific and sensitive methods based on mice model.
文摘RECOMBINANT GLYCOPROTEINS AS NOVEL ANTIGENIC TARGETS FOR DIAGNOSIS OF HEMOLYTIC UREMIC SYNDROME Human infection with Shiga toxin-producing Escherichia coli(STEC)is a major cause of postdiarrheal hemolytic uremic syndrome(HUS),a life-threatening condition characterized by hemolytic anemia,thrombocytopenia and acute renal failure.E.coli O157:H7 is the dominant STEC serotype associated with HUS worldwide although non-O157 STEC serogroups can cause a similar disease.Detection of anti-O157 LPS antibodies in combination with bacteriological procedures considerably improves diagnosis of STEC infections.
