In recent years, the demand for goat products has been growing due to the fact that goat milk has a number of advantages over cow milk, for example, it is low in lactose, and is considered less allergenic and easier t...In recent years, the demand for goat products has been growing due to the fact that goat milk has a number of advantages over cow milk, for example, it is low in lactose, and is considered less allergenic and easier to digest. To increase production during both breeding and non-breeding seasons and reduce the price of dairy products, it is necessary to effectively use reproductive management and assisted reproductive technologies. In vitro embryo production makes it possible to obtain a large number of eggs from goats, which for some reason are unable to conceive, but have genetic value. Afterward in vitro produced embryos can be transferred into recipient goats of other less genetically valuable breeds, such as the Ukrainian local breed. Therefore, the aim of the present study was to investigate the effectiveness of transfers of in vitro produced embryos of Saanen goats into surrogate sires of the Ukrainian local breed in different seasons. All manipulations with animals were carried out following ethical standards (Strasbourg, 1986). Six Saanen goats were selected as the oocyte donors. After the hormonal stimulation oocytes were retrieved by laparoscopic ovum pick-up. In vitro produced embryos were transferred laparotomically into 24 recipients of Ukrainian local breed. Fifty days after embryo transfers, pregnancies were determined by ultrasound diagnostics. Although the embryo development rate in the breeding season was 20% higher than in the non-breeding season, there was no difference in pregnancy and kidding rates between seasons. In conclusion, the transfer of in vitro produced Saanen goat embryos to recipients of the Ukrainian local breed gives the opportunity to achieve pregnancy and kidding regardless of the breeding season, which will enable a faster and more efficient increase in the livestock of highly productive goats in Ukraine in the post-war period.展开更多
Figs (Moracea: Ficus) and fig wasps (Hymenoptera: Chlocloids: Agaonideae) depend on each other to complete their reproduction. Monoecious fig species and their pollinating wasps are in conflict over the use of fig ov...Figs (Moracea: Ficus) and fig wasps (Hymenoptera: Chlocloids: Agaonideae) depend on each other to complete their reproduction. Monoecious fig species and their pollinating wasps are in conflict over the use of fig ovaries which can either produce one seed or one wasp. From observation on Ficus virens Ait., we showed that female flowers with outer layer of ovaries (near to the wall of syconium) had no significant difference from that with inner and interval layer of ovaries (near to the syconium cavity), in which most seeds and wasps were produced. This meant that fig tree provided the same potential resource for seed and wasps production. Observation indicated that there was usually only one foundress in syconium at female flower phase and no com- petition pollinators. Measurement of the style length of female flowers and the ovipositor of pollinators indicated that most ovaries could be reached by pollinator’s ovipositor. However, at the male flower phase, production of seeds was significantly more than that of wasps including non-pollinating wasps but there was no significant difference between seed and pollinating wasp production when without non-pollinating wasps produced. This result indicated that non-pollinating wasps competed ovaries not with seeds but with pollinating wasps for ovipositing. Bagged experiment showed that the sampling fig species was not self-sterile which was important for figs and wasps to survive bad season. Seed production in self-pollinated figs was not significantly different from total wasps in- cluding non-pollinating ones. This might be related with the weaker competition among wasps since bagged figs were not easy to reach by wasps from outside.展开更多
Three species ofnymphalid butterflies, Vanessa cardui, V. indica and Nymphalis xanthomelasjaponica, do not exhibit seasonal polyphenism in wing coloration. To deter-mine whether seasonal non-polyphenic butterflies pos...Three species ofnymphalid butterflies, Vanessa cardui, V. indica and Nymphalis xanthomelasjaponica, do not exhibit seasonal polyphenism in wing coloration. To deter-mine whether seasonal non-polyphenic butterflies possess a cerebral factor affecting wing coloration, we used a Polygonia c-aureurn female short-day pupal assay for detection of summer-morph-producing hormone （SMPH） activity in P. c-aureum. When 2% NaCl extracts of 25 brain-equivalents prepared from the pupal brains of V. cardui, V. indica or N. xanthomelasjaponica were injected into Polygonia female short-day pupae, all recipients developed into summer-morph adults with dark-yellow wings, and the average grade score （AGS） of summer morphs showing SMPH activity was 3.8, 3.7 and 4.0, respectively. In contrast, when acetone or 80% ethanol extracts prepared from pupal brains were injected into Polygonia pupae, all recipients developed into autumn-morph adults with a dark-brown coloration and each exhibited an AGS of less than 0.5. Our results indicate that a cerebral factor showing SMPH activity is present in the pupal brain of seasonal non-polyphenic nymphalid butterflies, suggesting that a SMPH and cerebral factor showing SMPH activity occur widely among butterfly species. This finding will improve our understanding of the presence of cerebral factors showing interspecific actions of SHPH.展开更多
该研究利用电感耦合等离子体质谱(inductively coupled plasma mass spectrometry,ICP-MS)对青岛市、合肥市和杭州市三地茶鲜叶中的9种金属元素(Na、K、Ca、Fe、Co、Cu、Zn、Mg和Mo)含量进行检测。利用单因素方差分析、多因素方差分析和...该研究利用电感耦合等离子体质谱(inductively coupled plasma mass spectrometry,ICP-MS)对青岛市、合肥市和杭州市三地茶鲜叶中的9种金属元素(Na、K、Ca、Fe、Co、Cu、Zn、Mg和Mo)含量进行检测。利用单因素方差分析、多因素方差分析和Pearson相关分析,探究产地、品种、季节及其交互作用对茶鲜叶中金属元素含量的影响,解析茶鲜叶中的金属元素含量与种源土壤中相关元素含量间的关系。结果显示:茶鲜叶中的金属元素含量在不同产地、品种和季节均具有各自的特征;除Mg、Zn和Mo外,茶鲜叶中其他金属元素的含量均与种源土壤显著相关。茶鲜叶中Na含量变异与产地×品种及产地×季节交互作用密切相关;Mg、K、Ca和Zn的含量变异与季节密切相关;Fe和Cu的含量变异与产地密切相关;Co含量变异与产地和产地×品种交互作用密切相关,而Mo含量变异与季节×品种及产地×品种×季节密切相关。研究表明,茶鲜叶中的金属元素含量变异与产地、品种、季节及其交互作用密切相关。展开更多
文摘In recent years, the demand for goat products has been growing due to the fact that goat milk has a number of advantages over cow milk, for example, it is low in lactose, and is considered less allergenic and easier to digest. To increase production during both breeding and non-breeding seasons and reduce the price of dairy products, it is necessary to effectively use reproductive management and assisted reproductive technologies. In vitro embryo production makes it possible to obtain a large number of eggs from goats, which for some reason are unable to conceive, but have genetic value. Afterward in vitro produced embryos can be transferred into recipient goats of other less genetically valuable breeds, such as the Ukrainian local breed. Therefore, the aim of the present study was to investigate the effectiveness of transfers of in vitro produced embryos of Saanen goats into surrogate sires of the Ukrainian local breed in different seasons. All manipulations with animals were carried out following ethical standards (Strasbourg, 1986). Six Saanen goats were selected as the oocyte donors. After the hormonal stimulation oocytes were retrieved by laparoscopic ovum pick-up. In vitro produced embryos were transferred laparotomically into 24 recipients of Ukrainian local breed. Fifty days after embryo transfers, pregnancies were determined by ultrasound diagnostics. Although the embryo development rate in the breeding season was 20% higher than in the non-breeding season, there was no difference in pregnancy and kidding rates between seasons. In conclusion, the transfer of in vitro produced Saanen goat embryos to recipients of the Ukrainian local breed gives the opportunity to achieve pregnancy and kidding regardless of the breeding season, which will enable a faster and more efficient increase in the livestock of highly productive goats in Ukraine in the post-war period.
基金Supported by the Knowledge Innovation Research Program,Chinese Academy of Sciences (KSCX2-SW-105)
文摘Figs (Moracea: Ficus) and fig wasps (Hymenoptera: Chlocloids: Agaonideae) depend on each other to complete their reproduction. Monoecious fig species and their pollinating wasps are in conflict over the use of fig ovaries which can either produce one seed or one wasp. From observation on Ficus virens Ait., we showed that female flowers with outer layer of ovaries (near to the wall of syconium) had no significant difference from that with inner and interval layer of ovaries (near to the syconium cavity), in which most seeds and wasps were produced. This meant that fig tree provided the same potential resource for seed and wasps production. Observation indicated that there was usually only one foundress in syconium at female flower phase and no com- petition pollinators. Measurement of the style length of female flowers and the ovipositor of pollinators indicated that most ovaries could be reached by pollinator’s ovipositor. However, at the male flower phase, production of seeds was significantly more than that of wasps including non-pollinating wasps but there was no significant difference between seed and pollinating wasp production when without non-pollinating wasps produced. This result indicated that non-pollinating wasps competed ovaries not with seeds but with pollinating wasps for ovipositing. Bagged experiment showed that the sampling fig species was not self-sterile which was important for figs and wasps to survive bad season. Seed production in self-pollinated figs was not significantly different from total wasps in- cluding non-pollinating ones. This might be related with the weaker competition among wasps since bagged figs were not easy to reach by wasps from outside.
文摘Three species ofnymphalid butterflies, Vanessa cardui, V. indica and Nymphalis xanthomelasjaponica, do not exhibit seasonal polyphenism in wing coloration. To deter-mine whether seasonal non-polyphenic butterflies possess a cerebral factor affecting wing coloration, we used a Polygonia c-aureurn female short-day pupal assay for detection of summer-morph-producing hormone （SMPH） activity in P. c-aureum. When 2% NaCl extracts of 25 brain-equivalents prepared from the pupal brains of V. cardui, V. indica or N. xanthomelasjaponica were injected into Polygonia female short-day pupae, all recipients developed into summer-morph adults with dark-yellow wings, and the average grade score （AGS） of summer morphs showing SMPH activity was 3.8, 3.7 and 4.0, respectively. In contrast, when acetone or 80% ethanol extracts prepared from pupal brains were injected into Polygonia pupae, all recipients developed into autumn-morph adults with a dark-brown coloration and each exhibited an AGS of less than 0.5. Our results indicate that a cerebral factor showing SMPH activity is present in the pupal brain of seasonal non-polyphenic nymphalid butterflies, suggesting that a SMPH and cerebral factor showing SMPH activity occur widely among butterfly species. This finding will improve our understanding of the presence of cerebral factors showing interspecific actions of SHPH.
文摘该研究利用电感耦合等离子体质谱(inductively coupled plasma mass spectrometry,ICP-MS)对青岛市、合肥市和杭州市三地茶鲜叶中的9种金属元素(Na、K、Ca、Fe、Co、Cu、Zn、Mg和Mo)含量进行检测。利用单因素方差分析、多因素方差分析和Pearson相关分析,探究产地、品种、季节及其交互作用对茶鲜叶中金属元素含量的影响,解析茶鲜叶中的金属元素含量与种源土壤中相关元素含量间的关系。结果显示:茶鲜叶中的金属元素含量在不同产地、品种和季节均具有各自的特征;除Mg、Zn和Mo外,茶鲜叶中其他金属元素的含量均与种源土壤显著相关。茶鲜叶中Na含量变异与产地×品种及产地×季节交互作用密切相关;Mg、K、Ca和Zn的含量变异与季节密切相关;Fe和Cu的含量变异与产地密切相关;Co含量变异与产地和产地×品种交互作用密切相关,而Mo含量变异与季节×品种及产地×品种×季节密切相关。研究表明,茶鲜叶中的金属元素含量变异与产地、品种、季节及其交互作用密切相关。
