Preliminary research and scheme design of deep underground in situ geo-information detection experiment for Geology in Time

The deep earth,deep sea,and deep space are the main parts of the national“three deep”strategy,which is in the forefront of the strategic deployment clearly defined in China’s 14th Five-Year Plan(2021-2025)and the Long-Range Objectives Through the Year 2035.It is important to reveal the evolutionary process and mechanism of deep tectonics to understand the earth’s past,present and future.The academic con-notation of Geology in Time has been given for the first time,which refers to the multi-field evolution response process of geological bodies at different time and spatial scales caused by geological processes inside and outside the Earth.Based on the deep in situ detection space and the unique geological envi-ronment of China Jinping Underground Laboratory,the scientific issue of the correlation mechanism and law between deep internal time-varying and shallow geological response is given attention.Innovative research and frontier exploration on deep underground in situ geo-information detection experiments for Geology in Time are designed to be carried out,which will have the potential to explore the driving force of Geology in Time,reveal essential laws of deep earth science,and explore innovative technologies in deep underground engineering.

Acoustic Non-Destructive Testing Technology in Concrete Bridge Inspection and Pile Foundation Detection

This article takes the actual construction project of a certain concrete bridge project as an example to analyze the application of acoustic non-destructive testing technology in its detection.It includes an overview of a certain bridge construction project studied and acoustic non-destructive testing technology and the application of acoustic non-destructive testing technology in actual testing.This analysis hopes to provide some guidelines for acoustic non-destructive testing of modern concrete bridge projects.

THE PRELIMINARY APPLICATION OF IN SITU HYBRIDI-ZATION IN DETECTING PROTO-ONCOGENES EXPRESSION IN HUMAN LEUKEMIC CELLS

An in situ hybridization technique with 35S labelled proto-oncogene probes (c-myc & c-fes) was used to detect their expression in bone marrow cells of 22 cases of leukemia of various types and immature granulocytes and erythroblasts of 16 nomal myelograms as controls. Both c-myc and c-fes were detectable in leukemic cells as well as in immature granulocytes and erythroblasts of normal bone marrow, but the expression extent varied in different cases. The levels of c-myc expression in leukemic cells were higher than those in controls (P<0.001). There was no difference of c-fes expression in two groups of bone marrow cells (P>0.05). This technique provides us a new method in studying variations of proto-oncogene expression in leukemic cells.

Specific Detection of Toxigenic Vibrio cholerae Based on in situ PCR in Combination With Flow Cytometry

Objective To develop an in situ PCR in combination with flow cytometry （ISPCR-FCM） for monitoring cholera toxin positive Vibrio cholerae. Methods In running this method, 4% paraformaldehyde was used to fix the Vibrio cholerae cells and 1 mg/mL lysozyme for 20 min to permeabilize the cells. Before the PCR thermal cycling, 2.5% glycerol was added into the PCR reaction mixture in order to protect the integrality of the cells. Results A length of 1037bp DNA sequence was amplified, which is specific for the cholera toxin gene （ctxAB gene）. Cells subjected to ISPCR showed the presences of ctxAB gene both in epifluorescence microscopy and in flow cytometric analysis. The specificity and sensitivity of the method were investigated. The sensitivity was relatively low （10^5 cells/mL）, while the specificity was high. Conclusion We have successfully developed a new technique for detection of toxigenic Vibrio cholerae strains. Further study is needed to enhance its sensitivities. ISPCR-FCM shows a great promise in monitoring specific bacteria and their physiological states in environmental samples.

Application of fluorescence in situ hybridization in the detection of bladder transitional-cell carcinoma: A multi-center clinical study based on Chinese population

Objective:To evaluate the diagnostic value of fluorescence in situ hybridization(FISH)in bladder cancer.Methods:We enrolled healthy volunteers and patients who were clinically suspected to have bladder cancer and conducted FISH tests and cytology examinations from August 2007 to December 2008.Receiver operating characteristic(ROC)curve analysis was performed and the area under curve(AUC)values were calculated for both the FISH and urine cytology tests.Results:A cohort of 988 healthy volunteers was enrolled to establish a reference range for the normal population.A total of 4807 patients with hematuria were prospectively,randomly enrolled for the simultaneous analysis of urine cytology,FISH testing,and a final diagnosis as determined by the pathologic findings of a biopsy or a surgically-excised specimen.Overall,the sensitivity of FISH in detecting transitional-cell carcinoma was 82.7%,while that of cytology was 33.4%(p<0.001).The sensitivity values of FISH for non-muscle invasive and muscle invasive bladder transitional-cell carcinoma were 81.7%and 89.6%,respectively(p=0.004).The sensitivity values of FISH for low and high grade bladder cancer were 82.6%and 90.1%,respectively(p=0.002).Conclusion:FISH is significantly more sensitive than voided urine cytology for detecting bladder cancer in patients evaluated for gross hematuria at all cancer grades and stages.Higher sensitivity using FISH was obtained in high grade and muscle invasive tumors.

Optical techniques in non-destructive detection of wheat quality:A review

Wheat quality detection is essential to ensure the safety ofwheat circulation and storage.The traditional wheat quality detection methods mainly include artificial sensory evaluation and physicochemical index analysis,which are difficult to meet the requirements for high accuracy and efficiency in modern wheat quality detection due to the disadvantages of subjectivity,destruction of sample integrity and low efficiency.With the rapid development of optical technology,various optical-based methods,using near-infrared spectroscopy technology,hyperspectral imaging technology and terahertz,etc.,have been proposed for wheat quality detection.These methods have the characteristics of nondestructiveness and high efficiency which make them popular in wheat quality detection in recent years.In this paper,various state-of-the-art optical-based techniques of wheat quality detection are analyzed and summarized in detail.Firstly,the principle and process of common optical non-destructive detection methods for wheat quality are introduced.Then,the optical techniques used in these detection methods are divided into seven categories,and the comparison of these technologies and their advantages and disadvantages are further discussed.It shows that terahertz technology is regarded as the most promising wheat quality detection method compared with other optical detection technologies,because it can not only detect most types of wheat deterioration,but also has higher accuracy and efficiency.Finally,the research of optical technology in wheat quality detection is prospected.The future research of optical technology-based wheat quality detection mainly includes the construction of wheat quality optical detection standardization database,the fusion of multiple optical detection technologies and multiple quality index information,the improvement of the anti-interference of optical technology and the industrialization of optical inspection technology for wheat quality.These studies are of great significance to improve the detection technology of wheat and ensure the storage safety of wheat in the future.

Optical generation,detection and non-destructive testing applications of terahertz waves

Optoelectronic terahertz generation and detection play a key role in the applications of non-destructive testing,which involves different areas such as physics,biological,material science,imaging,explosions detection,astronomy applications,semiconductor technology and superconductiong electronics. In this article,we present a reviewof the principle and performance of typical terahertz sources,detectors and non-destructive testing applications. On this basis,the newdevelopment and trends of terahertz radiation detectors are also discussed.

Study of pressure effects on ocean in-situ detection using laser-induced breakdown spectroscopy

Laser-induced breakdown spectroscopy(LIBS) has attracted extensive attention as a new technique for in-situ marine application. In this work, the influence of deep-sea high pressure environment on LIBS signals was investigated by using a compact LIBS-sea system developed by Ocean University of China for the in-situ chemical analysis of seawater. The results from the field measurements show that the liquid pressure has a significant effect on the LIBS signals. Higher peak intensity and larger line broadening were obtained as the pressure increases. By comparing the variations of the temperature and salinity with the LIBS signals, a weak correlation between them can be observed. Under high pressure conditions, the optimal laser energy was higher than that in air environment. When the laser energy exceeded 17 mJ, the effect of laser energy on the signal intensity weakened. The signal intensity decreases gradually at larger delays. The obtained results verified the feasibility of the LIBS technique for the deep-sea in-situ detection, and we hope this technology can contribute to surveying more deep-sea environments such as the hydrothermal vent regions.

In Situ Raman Monitoring of Trace Antibiotics in Different Harsh Water Environments

In situ surface-enhanced Raman scattering(SERS)is a widely used operando analytical technique,while facing numerous complex factors in applications under aqueous environment,such as low detection sensitivity,poor anti-interference capability,etc.,resulting in unreliable detectability.To address these issues,herein a new hydrophobic SERS strategy has been attempted.By comprehensively designing and researching a SERS-active structure of superhydrophobic ZnO/Ag nanowires,we demonstrate that hydrophobicity can not only draw analytes from water onto substrate,but also adjust"hottest spot"from the bottom of the nanowires to the top.As a result,the structure can simultaneously concentrate the dispersed molecules in water and the enhanced electric field in structure into a same zone,while perfecting its own anti-interference ability.The underwater in situ analytical enhancement factor of this platform is as high as 1.67×10^(11),and the operando limited of detection for metronidazole(MNZ)reaches to 10^(-9)M.Most importantly,we also successfully generalized this structure to various real in situ detection scenarios,including on-site detection of MNZ in corrosive urine,real-time warning of wrong dose of MNZ during intravenous therapy,in situ monitoring of MNZ in flowing wastewater with particulate interference,etc.,demonstrating the great application potential of this hydrophobic platform.This work realizes a synergistic promotion for in situ SERS performance under aqueous environment,and also provides a novel view for improving other in situ analytical techniques.

Multi-Energy Gamma-Ray Attenuations for Non-Destructive Detection of Hazardous Materials

We present a non-destructive method (NDM) to identify minute quantities of high atomic number (Z) elements in containers such as passenger baggage, goods carrying transport trucks, and environmental samples. This method relies on the fact that photon attenuation varies with its energy and properties of the absorbing medium. Low-energy gamma-ray intensity loss is sensitive to the atomic number of the absorbing medium, while that of higher-energies vary with the density of the medium. To verify the usefulness of this feature for NDM, we carried out simultaneous measurements of intensities of multiple gamma rays of energies 81 to 1408 keV emitted by sources 133Ba (half-life = 10.55 y) and 152Eu (half-life = 13.52 y). By this arrangement, we could detect minute quantities of lead and copper in a bulk medium from energy dependent gamma-ray attenuations. It seems that this method will offer a reliable, low-cost, low-maintenance alternative to X-ray or accelerator-based techniques for the NDM of high-Z materials such as mercury, lead, uranium, and transuranic elements etc.

Synthesis of Cu2O/Ag Composite with Visible Light Photocatalytic Degradation Activity for in situ SERS Analysis

A multifunctional Cu2O/Ag micro-nanocomposite, which has the characteristics of high cat- alytic activities under the visible light and high surface-enhanced Raman scattering （SERS） activity, was fabricated via a facile method and employed for the in situ SERS monitoring of the photocatalytic degradation reaction of crystal violet. Through the variation of the AgNO3 concentration, Ag content on the Cu2O template can be controllably tuned, which has great influence on the SERS effect. The results indicate that Ag nanopartieles form on the Cu2O nanoframes to obtain the Cu2O/Ag nanoeomposite, which can act as an excellent bifunetional platform for in situ monitoring of photocatalytic degradation of organic pollutions by SERS.

A fast and adaptive method for automatic weld defect detection in various real-time X-ray imaging systems

A first and effective method is proposed to detect weld deject adaptively in various Dypes of real-time X-ray images obtained in different conditions. After weld extraction and noise reduction, a proper template of median filter is used to estimate the weld background. After the weld background is subtracted from the original image, an adaptite threshold segmentation algorithm is proposed to obtain the binary image, and then the morphological close and open operation, labeling algorithm and fids＇e alarm eliminating algorithm are applied to pracess the binary image to obtain the defect, ct detection result. At last, a fast realization procedure jbr proposed method is developed. The proposed method is tested in real-time X-ray image,s obtairted in different X-ray imaging sutems. Experiment results show that the proposed method is effective to detect low contrast weld dejects with few .false alarms and is adaptive to various types of real-time X-ray imaging systems.

A novel 4D resolution imaging method for low and medium atomic number objects at the centimeter scale by coincidence detection technique of cosmic-ray muon and its secondary particles

The muon radiography imaging technique for high-atomic-number objects(Z)and large-volume objects via muon transmission imaging and muon multiple scattering imaging remains a popular topic in the field of radiation detection imaging.However,few imaging studies have been reported on low and medium Z objects at the centimeter scale.This paper presents an imaging system that consists of three layers of a position-sensitive detector and four plastic scintillation detectors.It acquires data by coincidence detection technique of cosmic-ray muon and its secondary particles.A 3D imaging algorithm based on the density of the coinciding muon trajectory was developed,and 4D imaging that takes the atomic number dimension into account by considering the secondary particle ratio information was achieved.The resultant reconstructed 3D images could distinguish between a series of cubes with 5-mm-side lengths and 2-mm-intervals.If the imaging time is more than 20 days,this method can distinguish intervals with a width of 1 mm.The 4D images can specify target objects with low,medium,and high Z values.

Current Trends and Perspectives of Detection and Location for Buried Non-Metallic Pipelines

Buried pipelines are an essential component of the urban infrastructure of modern cities.Traditional buried pipes are mainly made of metal materials.With the development of material science and technology in recent years,non-metallic pipes,such as plastic pipes,ceramic pipes,and concrete pipes,are increasingly taking the place of pipes made from metal in various pipeline networks such as water supply,drainage,heat,industry,oil,and gas.The location technologies for the location of the buried metal pipeline have become mature,but detection and location technologies for the non-metallic pipelines are still developing.In this paper,current trends and future perspectives of detection and location of buried non-metallic pipelines are summarized.Initially,this paper reviews and analyzes electromagnetic induction technologies,electromagnetic wave technologies,and other physics-based technologies.It then focuses on acoustic detection and location technologies,and finally introduces emerging technologies.Then the technical characteristics of each detection and location method have been compared,with their strengths and weaknesses identified.The current trends and future perspectives of each buried non-metallic pipeline detection and location technology have also been defined.Finally,some suggestions for the future development of buried non-metallic pipeline detection and location technologies are provided.

Simple synthesis of silver nanocluster composites AgNCs@PE-g-PAA by irradiation method and fluorescence detection of Cr^(3+)

Silver nanoclusters(AgNCs)are a new type of nanomaterials with similar properties to molecules and unique applications.The applications of AgNCs can be significantly expanded by combining them with different matrix materials to obtain AgNC composites.Using irradiation techniques,we developed a simple two-step method for preparing silver nanocluster composites.First,polyacrylic acid(PAA)chains were grafted onto the surface of a PE film as templates(PE-g-PAA).Subsequently,silver ions were reduced in situ on the surface of the template material to obtain the AgNC composites(AgNCs@PE-g-PAA).The degree of AgNC loading on the composite film was easily controlled by adjusting the reaction conditions.The loaded AgNCs were anchored to the carboxyl groups of the PAA and wrapped in the graft chain.The particle size of the AgNCs was only 4.38±0.85 nm,with a very uniform particle size distribution.The AgNCs@PE-g-PAA exhibited fluorescence characteristics derived from the AgNCs.The fluorescence of the AgNCs@PE-g-PAA was easily quenched by Cr^(3+)ions.The composite can be used as a fluorescence test paper to realize visual detection of Cr^(3+).

Higher-Order Statistics for Automatic Weld Defect Detection

Image processing and image analysis are the main aspects for obtaining information from digital image owing to the fact that this techniques give the desired details in most of the applications generally and Non-Destructive testing specifically. This paper presents a proposed method for the automatic detection of weld defects in radiographic images. Firstly, the radiographic images were enhanced using adaptive histogram equalization and are filtered using mean and wiener filters. Secondly, the welding area is selected from the radiography image. Thirdly, the Cepstral features are extracted from the Higher-Order Spectra (Bispectrum and Trispectrum). Finally, neural networks are used for feature matching. The proposed method is tested using 100 radiographic images in the presence of noise and image blurring. Results show that in spite of time consumption, the proposed method yields best results for the automatic detection of weld defects in radiography images when the features were extracted from the Trispectrum of the image.

Chromosome 11 aneusomy in esophageal cancers and precancerous lesions-an early event in neoplastic transformation:An interphase fluorescence in situ hybridization study from south India

AIM: To detect aneusomic changes with respect to chromosome 11 copy number in esophageal precancers and cancers wherein the generation of cancer-specific phenotypes is believed to be associated with specific chromosomal aneuploidies. METHODS: We performed fluorescence in situ hybridization (FISH) on esophageal tissue paraffin sections to analyze changes in chromosome 11 copy number using apotome-generated images by optical sectioning microscopy. Sections were prepared from esophageal tumor tissue, tissues showing preneoplastic changes and histologically normal tissues (control) obtained from patients referred to the clinic for endoscopic evaluation. RESULTS: Our results demonstrated that aneusomy was seen in all the cancers and preneoplastic tissues, while none of the controls showed aneusomic cells. There was no increase in aneusomy from precancers to cancers. CONCLUSION: Our results suggest that evaluation of chromosome 11 aneusomy in esophageal tissue using FISH with an appropriate signal capture-analysis system, can be used as an ancillary molecular marker predictive of early neoplastic changes. Future studies can be directed towards the genes on chromosome 11,which may play a role in the neoplastic transformation of esophageal precancerous lesions to cancers.

An Automated Ultrasonic NDT System for In-Situ Inspection of Wind Turbine Blades

It is crucial to maintain wind turbine blades regularly, due to the high stress leading to defects or damage. Conventional methods require shipping the blades to a workshop for off-site inspection, which is extremely time-consuming and very costly. This work investigates the use of pulse-echo ultrasound to detect internal damages in wind turbine blades without the necessity to ship the blades off-site. A prototype 2D ultrasonic NDT （non-destructive testing） system has been developed and optimised for in-situ wind turbine blade inspection. The system is designed to be light weight so it can be easily carried by an inspector onto the wind turbine blade for in-situ inspection. It can be operated in 1D A-scan, 2D C-scan or 3D volume scan. A software system has been developed to control the automated scanning and show the damage areas in a 2D/3D map with different colours so that the inspector can easily identify the defective areas. Experiments on GFRP （glass fibre reinforced plastics） and wind turbine blades （made of GFRP） samples showed that internal defects can be detected. The main advantages of this system are fully automated 2D spatial scanning and the ability to alert the user to the damage of the inspected sample. It is intended to be used for in-situ inspection to save maintenance time and hence considered to be economically beneficial for the wind energy industry.

月面原位水资源获取技术与发展趋势

随着深空探测活动的持续推进,月球探测已成为开展行星际探测的重要部分.月球原位资源利用是保障载人月球探测及月面长期驻留的关键技术途径,中国已将月面原位水获取方法列为月球探测的关键技术之一.月面原位水获取方法主要分为极区水冰勘探开采和月壤氢还原制水两种技术途径.现有勘探到的水冰资源主要位于月球极区,分布不均匀,开采难度大.目前研究提出了多种不同类型的极区水冰勘探开采方法,但实际效果有待月面原位试验验证.月壤加氢还原制水技术方法工作条件不受区域限制,应用范围较广,但仍存在反应条件要求高、能耗大等技术限制,未来需在节能和有效矿物成分富集等方面有所突破.