A CuNi/C Nanosheet Array Based on a Metal–Organic Framework Derivate as a Supersensitive Non-Enzymatic Glucose Sensor

Bimetal catalysts are good alternatives for nonenzymatic glucose sensors owing to their low cost, high activity, good conductivity, and ease of fabrication. In the present study, a self-supported CuNi/C electrode prepared by electrodepositing Cu nanoparticles on a Ni-based metal–organic framework(MOF) derivate was used as a non-enzymatic glucose sensor. The porous construction and carbon scaffold inherited from the Ni-MOF guarantee good kinetics of the electrode process in electrochemical glucose detection. Furthermore, Cu nanoparticles disturb the array structure of MOF derived films and evidently enhance their electrochemical performances in glucose detection. Electrochemical measurements indicate that the CuNi/C electrode possesses a high sensitivity of17.12 mA mM^(-1) cm^(-2), a low detection limit of 66.67 nM,and a wider linearity range from 0.20 to 2.72 mM. Additionally, the electrode exhibits good reusability, reproducibility, and stability, thereby catering to the practical use of glucose sensors. Similar values of glucose concentrations in human blood serum samples are detected with our electrode and with the method involving glucose-6-phosphate dehydrogenase; the results further demonstrate the practical feasibility of our electrode.

A novel sensitive non-enzymatic glucose sensor

Cu nanoclusters were electrochemically deposited on the film of a Nafion-solubilized multi-wall carbon nanotubes (CNTs) modified glassy carbon electrode (CNTs-GCE), which fabricated a Cu-CNTs composite sensor (Cu-CNTs-GCE) to detect glucose with non-enzyme. The linear range is 7.0 × 10?7 to 3.5 × 10?3 mol/L with a high sensitivity of 17.76 μA/(mmol L), with a low detection limit 2.1 × 10?7 mol/L, fast response time (within 5 s), good reproducibility and stability.

Non-Enzymatic Glucose Sensor Based on the Novel Flower Like Morphology of Nickel Oxide

In this study, novel nickel oxide (NiO) flowers like nanostructures were fabricated onto gold coated glass substrate by hydrothermal method using high alkaline pH medium. The structural study of nickel oxide nanostructures was performed by scanning electron microscopy (SEM) and X-ray differaction (XRD) techniques. Nickel oxide nanostructures are highly dense, uniform and possess good crystalline quality. The so prepared structures were investigated for their electrochemical properties by cyclic voltammetry and amperometric techniques. The nickel oxide flower like morphology has shown good electrochemical performances for the oxidation of glucose. The presented sensing material was able to detected glucose in a wide range of concentration of 0.001 mM to 8 mM with a high sensitivity (123 μmA/mM) and regression coefficient of 0.99. Moreover, the NiO nanostructures based sensor is highly reproducible, stable, exhibiting a fast response time and selective in the response. All the obtained results indicate the potential use of this material in the development of enzyme free sensors for the detection of glucose.

Reusable electrochemical non-enzymatic glucose sensors based on Au-inlaid nanocages

Mass detection of glucose,which is required in many applications,remains challenging.The commercial enzyme-based glucose test strips cannot be reused,and current non-enzymatic glucose sensors exhibit a narrow range of detection and slow glucose oxidation kinetics.Herein,controlled etching of Prussian blue analogue(PBA)nanocubes at the vertices is conducted and Au nanoparticles(Au NPs)are subsequently inlaid in the etched cavities by in-situ reduction of HAuCl4.The unique AuNP-PBA nanocomplexes exhibit low electrochemical potential for glucose oxidation,high electrocatalytic activity,and rapid redox electron transfer rate.Covalent immobilization of the Au-inlaid nanomaterials on a fine Au wire leads to a non-enzymatic glucose sensor with a particularly wide linear detection range(10μM to 16 mM),excellent anti-interference,and fast response.More importantly,the sensor is reusable,and its sensitivity is well maintained even after 150 times of detection.This new-concept material promises to enable high-throughput glucose detection at a low cost,which is essential in diabetic management and other healthcare applications.

Facile synthesis of CuO-Co_(3)O_(4)prickly-sphere-like composite for non-enzymatic glucose sensors

In the field of glucose sensors,the development of inexpensive and high-efficiency electrochemical glucose sensors is the current research hotspot.In this paper,CuO-Co_(3)O_(4)composite with a prickly-sphere-like morphology is prepared by the facile hydrothermal method for the non-enzymatic electrochemical glucose detection.X-ray diffraction,scanning electron microscopy,transmission electron microscopy,energy-dispersive X-ray spec-troscopy,and X-ray photoelectron spectroscopy are used to analyze the structure,composition,and morphology of the material.In addition,the electrochemical catalytic perfor-mance of CuO-Co_(3)O_(4)to glucose is obtained by cyclic voltammetry and chronoamperometry.The excellent elec-trochemical sensing performance may be attributed to the large number of catalytic sites in the prickly-sphere-like composite and the synergistic effect of Cu and Co.Under an applied voltage of 0.55 V,CuO-Co_(3)O_(4)composite shows sensitivity to glucose(1503.45μA·(mmol·L^(-1))^(-1)-cm^(-2)),a low detection limit(21.95μmol·L^(-1)),excellent selectivity,a high level of reproducibility,and good sta-bility.This indicates that the CuO-Co_(3)O_(4)composite has a broad prospect of non-enzymatic glucose sensing application.

Sandwich structure confined gold as highly sensitive and stable electrochemical non-enzymatic glucose sensor with low oxidation potential

The preparation of highly sensitive and stable non-enzymatic glucose sensors is critical to the prevention and treatment of diabetes.Fe_(3)O_(4)@Au@Co Fe-LDH is prepared through a spontaneous galvanic displacement reaction.A series of structural characterizations testify the successful formation of Fe_(3)O_(4)@Au@Co FeLDH electrocatalyst,with the Au intercalating between Fe_(3)O_(4)and LDH to form the sandwich structure.Cyclic voltammetry tests indicate that Au is responsible for the electrocatalytic oxidation of glucose.The characterizations of the electrochemical sensor for glucose detection indicate that Fe_(3)O_(4)@Au@Co FeLDH possesses high sensitivity of 6342μA m M^(-1)cm^(-2),with an extremely low oxidation potential of 0.82 V vs.RHE.Even with the high glucose concentration of 15 m M,the sensitivity remains at 4359μA m M^(-1)cm^(-2).Due to the broad linear detection range(0.0375 to 15.64 m M)and the low limit of detection(12.7μM),Fe_(3)O_(4)@Au@Co Fe-LDH is applicable towards practical application.Thanks to the sandwich structure,which confines the Au in between Fe_(3)O_(4)and Co Fe-LDH,the Fe_(3)O_(4)@Au@Co Fe-LDH glucose sensor shows high long-term stability and satisfactory selectivity.The successful synthesis of the sandwichstructured Fe_(3)O_(4)@Au@Co Fe-LDH provides a new conception for the design of highly sensitive and stable non-enzymatic glucose electrodes.

Polydopamine-Based Colorimetric Superwettable Biosensor for Highly Sensitive Detection of Hydrogen Peroxide and Glucose

Superwettable surface has broad application prospects in fabricating biosensors due to its significant enrichment effect.Here,we report a polydopamine-based colorimetric superwettable sensor that integrates superhydrophobic-superhydrophilic micropatterns for the determination of hydrogen peroxide(H_(2)O_(2))and glucose.Dopamine can be oxidized into polydopamine with the addition of horseradish peroxidase(HRP)and H_(2)O_(2),leading to the deposited spots color change from colorless to black.The concentration of target can be determined by analyzing RGB value using a smartphone software.The superhydrophobic area on the superwettable surface helps capture droplets by confining them to superhydrophilic microwells.After droplet evaporation,the analytes are concentrated in the small superhydrophilic domain,thus greatly enhancing the sensitivity.The experimental results manifested that superwettable sensor is able to detect H_(2)O_(2)with a broad linear range of 0.25μmol/L-25 mmol/L and a low limit of detection(LOD)of 0.25μmol/L by naked eye.For glucose detection,the linear range of the sensor is from 2μmol/L to 20 mmol/L and LOD is 0.69μmol/L.The superwettable sensor has been successfully applied in practical samples,including cancerous cells,milk,urine,and human serum samples with acceptable results.This superwettable sensor has several merits,such as high sensitivity,rapid response,and low sample volume in a single microdroplet,and shows great potential in manufacturing portable devices for complex biosensing applications.

Electrochemical non-enzymatic glucose sensors based on nano-composite of Co3O4 and multiwalled carbon nanotube

Nanocomposite of Co3O4 and multiwalled carbon nanotube (MCNT) was synthesised using one step solvothermal method, and an electrochemical non-enzymatic glucose sensor (Co3O4-MCNT/GCE) was successfully constructed by a dropping method. The obtained Co3O4 and Co3O4- MCNT were characterized and investigated by transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDS). Quantitative analysis of glucose was performed using the amperometric (i–t) method, and plot of current difference versus concentration of glucose was linear in the range of 1.0–122μmol/L, with a linear correlation coefficient (R^2) of 0.9983 and limit of detection (LOD) of 0.28μmol/L. Sensitivity of this sensor was evaluated as 2550μA L mmol^-1 cm^-2. This new sensor produced satisfactory reproducibility and stability and was applied to monitor trace amounts of glucose in human serum samples.

Extended detection range for an optical enzymatic glucose sensor coupling with a novel data-processing method

A new data-processing method was established and applied for optical enzymatic glucose sensing,in which oxygen and glucose were simultaneously consumed.The oxygen level remaining in the detection system,which was equal to the difference between the initial and consumed oxygen concentrations,could be measured using fluorescent oxygen indicators immobilized in the sensing layer.It was deduced that the ratio of I0 and I was inversely proportional to glucose concentration,where I0 is the maximum fluorescence intensity in various glucose solutions,and I is the fluorescence intensity at various concentrations of glucose.Using the new data-processing method,the detection range of the calibration curve method was extended from 0 to 1.2 mmol L-1,which was enlarged about 2–3 folds over that in ordinary approaches.The prepared glucose sensor could be directly applied to detect high concentrations of glucose.

Sensing nanomaterials of wearable glucose sensors

The metabolic disorder of glucose in human body will cause diseases such as diabetes and hyperglycemia.Hence the determination of glucose content is very important in clinic diagnosing.In recent years,researchers have proposed various non-invasive wearable sensors for rapid and real-time glucose monitoring from human body fluids.Unlike those reviews which discussed performances,detection environments or substrates of the wearable glucose sensor,this review focuses on the sensing nanomaterials since they are the key elements of most wearable glucose sensors.The sensing nanomaterials such as carbon,metals,and conductive polymers are summarized in detail.And also the structural characteristics of different sensing nanomaterials and the corresponding wearable glucose sensors are highlighted.Finally,we prospect the future development requirements of sensing nanomaterials for wearable glucose sensors.This review would give some insights to the further development of wearable glucose sensors and the modern medical treatment.

Electrochemical sensor investigation of carbon-supported PdCoAg multimetal catalysts using sugar-containing beverages

Novel PdCoAg/C nanostructures were suc-cessfully synthesized by the polyol method in order to develop electrocatalysts,related to the glucose sensor performance of the high glycemic index in beverages.The characterization of this novel PdCoAg/C electrocatalyst was performed by X-ray diffraction,scanning electron microscopy,transmission clectron microscopy,and high-resolution transmission electron microscopy equipped with energy dispersive X-ray.The characterization results revealed that electronic state of the PdCoAg/C electro-catalyst was modified by the addition of the third metal.The electrochemical performances of the sensor were investigated by cyclic voltammetry and differential pulse voltammetry.The prepared enzyme-free sensor exhibited excellent catalytic activity against glucose with a wide detection range(0.005 to 0.35 mmol·L^-1),low limit of detection(0.003 mmol·L^-1),high sensitivity(4156.34μA·mmol^-1·L·cm^-3),and long-term stability(10 days)because of the synergistic effect between the termary metals.The glucose contents of several energy drinks,fruit juices,and carbonated beverages were analyzed using the novel PdCoAgNGCE/C sensor system.These results indicate the feasibility for applications in the foods industry.

一种新型二氧化硅多孔材料固定化酶光纤葡萄糖传感器的研究

目的 研制一种新型的基于氧敏感指示剂Ru(bpy)3Cl2和固定化葡萄糖氧化酶(GOD)的复合敏感膜光纤传感器,用于实时检测葡萄糖溶液质量浓度。方法 以多孔SiO_(2)纳米粒子为载体固定GOD,并采用溶胶-凝胶法同时加入Ru(bpy)_(3)Cl_(2)制备葡萄糖敏感膜,通过提拉法在光纤端面沉积敏感膜制成光纤传感探头,搭建光纤传感器用于实时检测葡萄糖溶液质量浓度。结果 光纤传感器检测葡萄糖溶液质量浓度为50~900mg/dL(2.78~50.00mmol/L),在用于实时监测葡萄糖溶液质量浓度变化时响应时间短,测试标准曲线线性良好。结论 SiO_(2)多孔材料固定化酶光纤葡萄糖传感器具有灵敏度高、响应速度快、检测范围大等优点,未来可以检测人体血液中的葡萄糖质量浓度。

三维葡萄糖电化学传感器与胶原水凝胶集成实时监测细胞代谢

葡萄糖代谢的实时监测不仅能够提供细胞生长状态信息,还有助于深入理解细胞代谢机制。电化学传感器已成为葡萄糖实时动态测量的重要方法之一,然而,目前报道的葡萄糖传感器大多基于二维电极,很难与三维细胞培养体系良好兼容并进行准确检测。通过在对H2O2还原具有催化作用的多孔电极表面固定葡萄糖氧化酶(GOD),制备了一种灵敏度高、选择性好的三维葡萄糖电化学传感器。然后,将包裹了肿瘤细胞(MCF-7)的胶原水凝胶填充到三维传感器孔隙,构建了兼具细胞三维培养和电化学检测于一体的集成平台。通过实时监测MCF-7细胞培养环境中葡萄糖的浓度变化,实现了肿瘤细胞在抗癌药物作用下葡萄糖代谢能力的实时分析,为细胞能量代谢行为研究提供了一种新的评估方法。

无酶葡萄糖检测的研究进展

本文主要介绍了各种无酶葡萄糖的检测方法。相对于酶法,该方法克服了由于酶本身固有的属性而导致的在固定化过程中不稳定易失活的缺点,具有较好的重现性、稳定性以及抗干扰能力。

用于血糖无创检测的葡萄糖传感器研究

研制一种新型葡萄糖传感器,初步实现用于反离子电渗透技术提取皮下组织液的葡萄糖的浓度测量。用铁氰化钾作为电子媒介体,固定在聚环氧乙烷凝胶里的葡萄糖氧化酶与溶液中的葡萄糖催化氧化生成葡萄糖酸和亚铁氰化钾,通过检测该反应产生的氧化还原电流的大小来计算葡萄糖溶液的浓度。新型葡萄糖传感器的检测浓度范围2～22 mmol/L内线性度较好,传感器的一致性测试表明:同一传感器多次测量的偏差不超过2%,反应时间较短,接近于1 s。

葡萄糖的六种荧光检测法研究

介绍了6种葡萄糖荧光检测法.详细介绍了每种检测方法的荧光强度变化机理和葡萄糖检测原理,并在此基础上对葡萄糖荧光传感器的发展趋势作了展望.

一种改进的能量代谢守恒法无创检测血糖算法

为了提升基于能量代谢守恒方法无创检测血糖的精度,设计新型无创血糖检测探头,采集300组临床试验数据,提出决策树和偏最小二乘法方法相结合的DE-PLS算法建立预测血糖值的模型。并采用多参数相关性分析,得出代谢率M和血糖浓度真值BG显著相关,验证了能量代谢守恒法无创检测血糖理论的可行性。最终结果表示,DE-PLS算法计算出的血糖浓度与真实血糖值的相关性达到了88.5%,证实该算法的精度高于应用于该领域的其他算法。

无酶血糖传感器研究进展

血糖监测对糖尿病患者具有重要的意义,发展快速、准确、实时的葡萄糖检测技术已成为当今社会研究的热点。本研究介绍了无酶葡萄糖传感器的概念与分类,详细阐述了无酶血糖传感器电极的表征方法和不同类型材料在无酶血糖传感器中的应用进展,并提出了目前无酶血糖传感器应用中存在的问题,对其未来应用前景进行了展望。

基于液芯MTC的低浓度血液葡萄糖光学传感器研究

为了实现超低样本体积、高灵敏度的血液葡萄糖浓度检测,采用时域有限差分法分析了液芯微管腔(MTC)的壁厚和直径对回音壁模式(WGM)共振特性的影响。利用熔融拉锥法制备了MTC,通过高精度电控位移平台实现MTC和锥形光纤的高精度耦合以及WGM共振谱的激发,并对MTC进行表面功能化和过耦合方法以提高灵敏和稳定性,同时进行了理论分析和实验验证。结果表明,表面功能化的液芯MTC传感器取得的灵敏度约为0.911pm/(mmol·L^(-1)),线性度为0.988;该低浓度血液葡萄糖光学传感器的灵敏度和稳定性很高。这一结果对运动员训练中血糖的实时、快速监测,保障运动安全性和持久性等是有帮助的。

电催化剂Pt-WO_3用作葡萄糖传感器工作电极性能的研究

用铂金属和溶解的三氧化钨电化学共沉积制备P_1/WO_3电极,利用流动分析等方法研究厂电极作为葡萄糖传感器工作电极的性能.结果表明:该电极具有工作电位低、催化活性高、响应快、重复性好、抗干扰能力强及使用寿命长等特点.在2×10^(-3)～4×1O^(-2)mol/L范围内.