The author used ear acupuncture to treat 52 cases with reaction to blood transfu-sion or fluid infusion and obtained a good and quick therapeutic effect, 50 cases (96. 2％) were curedand 2 cases (3. 8％) improved.
To study the diagnosis of hepatitis C viremia,17 patients(including 14 in acute phase)with post-transfusion non-A,non-B hepatitis(NANBH)were investigated by nested polymerasechain reaction after reverse transcription ...To study the diagnosis of hepatitis C viremia,17 patients(including 14 in acute phase)with post-transfusion non-A,non-B hepatitis(NANBH)were investigated by nested polymerasechain reaction after reverse transcription with 3 sets of primers complemented to different regions ofthe viral sequence.Hepatitis C virus(HCV)RNA was detected in 6(35%)cases with reverse tran-script polymerase chain reaction(RT-PCR)using the primers according to the Japanese clones;in13(76%)using the primers according to the non-coding region which is highly conserved in theviral sequence,and in 16(94%)using both or either one of these two sets of primers.However,no HCV RNA was detected with the primers according to the Chiron prototype.The nucleotide se-quence of HCV is quite variable,and the virus level in blood is very low,therefore,in order to getprecise detection,it is suggested to do nested RT-PCR with several sets of primers complementaryto different conserved regions.展开更多
Objective: To assess the clinical efficiency of the transfusion of leucocyte filtrated RBC concentrates to prevent febrile nonhemolytic transfusion reactions (FNHTRs). Methods: One hundred patients with liver cirrhosi...Objective: To assess the clinical efficiency of the transfusion of leucocyte filtrated RBC concentrates to prevent febrile nonhemolytic transfusion reactions (FNHTRs). Methods: One hundred patients with liver cirrhosis, gastric ulcer or cancer were subjected to receive RBC concentrates after leucocyte filtration. Another 50 patients with similar diseases were selected to receive non-filtrated RBC concentrates. The incidence of FNHTRs in all patients was investigated. Results: There was no FNHTR in 100 transfusions with leucocyte filtrated RBC concentrates, while FNHTRs occurred in 8 of 50 patients with non-filtrated RBC concentrates, with the incidence of 16%. Conclusion: FNHTRs to RBC transfusion can be prevented with leucocyte filtration.展开更多
INTRODUCTIONAlthough several specific detecting methods hadbeen applied to determine the hepatitis virus,therewas a lot of cryptogenic hepatitis without anyknown hepatitis infectious marker.Theprevalence of hepatitis ...INTRODUCTIONAlthough several specific detecting methods hadbeen applied to determine the hepatitis virus,therewas a lot of cryptogenic hepatitis without anyknown hepatitis infectious marker.Theprevalence of hepatitis G virus (HGV) (also knownas GB-C virus) infection has been reported to be 5%-13% in patients with non-A-E hepatitis andcirrhosis,however,there is little evidencesuggesting that HGV causes hepatitis in human.展开更多
Objective To investigate transfusion transmitted virus (TTV) infection among population of different groups in Shaanxi Province.Methods A nested polymerase chain reaction (PCR) with primers from ORF1 of TTV genome was...Objective To investigate transfusion transmitted virus (TTV) infection among population of different groups in Shaanxi Province.Methods A nested polymerase chain reaction (PCR) with primers from ORF1 of TTV genome was established to detect TTV DNA in serum of the patients.Results TTV DAN was detected in the sera of 3 of 50 cases of general population(6%), 2 of 30 cases of vocational blood donors(6.7%),21 of 97 cases with Type B hepatitis(21.6%),9 of 35 cases of Type C hepatitis (25.7%),and 23 of 40 cases with non A^non G hepatitis(57 5%).Conclusion There is TTV infection among general population in Shaanxi Province.TTV may be an important agent to cause non A^non G hepatitis .And the patients with HBV or HCV can have overlapping TTV infection.展开更多
文摘The author used ear acupuncture to treat 52 cases with reaction to blood transfu-sion or fluid infusion and obtained a good and quick therapeutic effect, 50 cases (96. 2％) were curedand 2 cases (3. 8％) improved.
文摘To study the diagnosis of hepatitis C viremia,17 patients(including 14 in acute phase)with post-transfusion non-A,non-B hepatitis(NANBH)were investigated by nested polymerasechain reaction after reverse transcription with 3 sets of primers complemented to different regions ofthe viral sequence.Hepatitis C virus(HCV)RNA was detected in 6(35%)cases with reverse tran-script polymerase chain reaction(RT-PCR)using the primers according to the Japanese clones;in13(76%)using the primers according to the non-coding region which is highly conserved in theviral sequence,and in 16(94%)using both or either one of these two sets of primers.However,no HCV RNA was detected with the primers according to the Chiron prototype.The nucleotide se-quence of HCV is quite variable,and the virus level in blood is very low,therefore,in order to getprecise detection,it is suggested to do nested RT-PCR with several sets of primers complementaryto different conserved regions.
文摘Objective: To assess the clinical efficiency of the transfusion of leucocyte filtrated RBC concentrates to prevent febrile nonhemolytic transfusion reactions (FNHTRs). Methods: One hundred patients with liver cirrhosis, gastric ulcer or cancer were subjected to receive RBC concentrates after leucocyte filtration. Another 50 patients with similar diseases were selected to receive non-filtrated RBC concentrates. The incidence of FNHTRs in all patients was investigated. Results: There was no FNHTR in 100 transfusions with leucocyte filtrated RBC concentrates, while FNHTRs occurred in 8 of 50 patients with non-filtrated RBC concentrates, with the incidence of 16%. Conclusion: FNHTRs to RBC transfusion can be prevented with leucocyte filtration.
基金Science Fund of Military Medical Science for the Ninth Five-Year Key Research,No.98Z073
文摘INTRODUCTIONAlthough several specific detecting methods hadbeen applied to determine the hepatitis virus,therewas a lot of cryptogenic hepatitis without anyknown hepatitis infectious marker.Theprevalence of hepatitis G virus (HGV) (also knownas GB-C virus) infection has been reported to be 5%-13% in patients with non-A-E hepatitis andcirrhosis,however,there is little evidencesuggesting that HGV causes hepatitis in human.
基金supported by Natural Science Foundation of Shaanxi Province(No.2000 SM56)
文摘Objective To investigate transfusion transmitted virus (TTV) infection among population of different groups in Shaanxi Province.Methods A nested polymerase chain reaction (PCR) with primers from ORF1 of TTV genome was established to detect TTV DNA in serum of the patients.Results TTV DAN was detected in the sera of 3 of 50 cases of general population(6%), 2 of 30 cases of vocational blood donors(6.7%),21 of 97 cases with Type B hepatitis(21.6%),9 of 35 cases of Type C hepatitis (25.7%),and 23 of 40 cases with non A^non G hepatitis(57 5%).Conclusion There is TTV infection among general population in Shaanxi Province.TTV may be an important agent to cause non A^non G hepatitis .And the patients with HBV or HCV can have overlapping TTV infection.
