To regenerate three-dimensional endometrium in vitro as a novel model for studying the mechanism of implantation of embryos, the luminal epithelial cells and stromal cells of the rabbit uterus were separated and cultu...To regenerate three-dimensional endometrium in vitro as a novel model for studying the mechanism of implantation of embryos, the luminal epithelial cells and stromal cells of the rabbit uterus were separated and cultured in vitro. The type Ⅰ mouse tail collagen was used as scaffolding material. The stromal cells were inoculated in the type I mouse tail collagen, and the luminal epithelial cells were inoculated on the type i mouse tall collagen to regenerate the endometrium in vitro. The regenerated endometrium was cultured in DMEM-F/12 media containing 100 nmol L^-1 progesterone, 10 nM β-estradiol, and 10% fetal bovine serum （FBS） for 3 d. The media were then replaced with CZB containing 100 nM progesterone, 10 nmol L-1 β-estradiol, and 10% FBS, and the mouse blastulas were co-cultured with it. The results of scanning electronic micrography showed that the epithelial cells on the surface of the reconstructed endometrium were covered with numerous slender microvilli and some epithelial cells protruded pinopodes. After culturing for 12 h with the mouse blastula, the shedding, attachment, and implantation of the blastula were observed. The blastula can escape from zona pellucida and attach to the three-dimensional endometrium and is then implanted into it. This study showed that the reconstructed three-dimensional endometrium can serve as a robust embryo implantation model in vitro.展开更多
Objective: We established a transplantation tumor model of human osteosarcoma in chick embryo, studied its morphological and biological characteristics, and observed its dynamic process of angiogenesis induction so th...Objective: We established a transplantation tumor model of human osteosarcoma in chick embryo, studied its morphological and biological characteristics, and observed its dynamic process of angiogenesis induction so that a simple and practical model can be provided for studying osteosarcoma. Methods: Human osteosarcoma cells at different concentrations were inoculated in chorioallantoic membrane (CAM) of chick embryos at different embryonic ages to observe the factors affecting the survival of the transplanted osteosarcoma in chick embryo, growth characteristics of the transplantation tumor, and the morphological characteristics and biological characteristics of the transplantation tumor. Results: The transplantation tumor model of human osteosarcoma in chick embryo was successfully established. It was found that the transplantation tumor was easy to grow and it showed strong angiogenesis-inducing effects. Under the light microscope, the transplantation tumor showed a similar tissue structure to human osteosarcoma. Conclusion: It is feasible to establish a transplantation tumor model of human osteosarcoma in chick embryo. The model can be easily duplicated with a simple operation, which provides a useful animal model for studying osteosarcoma.展开更多
[Objective] The aim was to study the effect of the content of copper and zinc on in medium the vitamin E accumulation in wheat embryo-dreived callus.[Method] The mathematical models were established to describe the gr...[Objective] The aim was to study the effect of the content of copper and zinc on in medium the vitamin E accumulation in wheat embryo-dreived callus.[Method] The mathematical models were established to describe the growth kinetics and the vitamin E accumulation in wheat embryo callus cells.With the aim of getting the highest accumulation of the secondary metabolite Vitamin E,the optimal combination of copper and zinc in medium was confirmed by testing.[Result] The results showed that the production of vitamin E in B5 medium reached the highest value with 2.0 mg/mL ZnSO4·7H2O and 0.1 mg/mL CuSO4·5H2O.The fitting degrees of kinetic models of vitamin E accumulation and cell growth were 97.53% and 95.60%,respectively,which indicated good nonlinear relationships.[Conclusion] Both copper and zinc could affect the accumulation of vitamin E in wheat germ callus,and Copper showed more prominent effect than Zn.Synergism existed in low copper and zinc concentration,and the inhibitive effect enhanced with the increase of the concentrations.展开更多
A DH population derived from C49S-87/01Y1-1069 was used to study the inheritance of wheat haploid embryo production frequency（EPF） in wheat × maize cross with the mixed major gene and polygene inheritance model...A DH population derived from C49S-87/01Y1-1069 was used to study the inheritance of wheat haploid embryo production frequency（EPF） in wheat × maize cross with the mixed major gene and polygene inheritance model of quantitative traits. The results showed that the EPF of wheat × maize cross was controlled by two dominant epistatic genes and polygene with gene effects of 1.95 for the first major gene, 6.69 for the second one and 2.80 for the polygene. The inheritability of major genes was as high as 72.09%, suggesting that the differences in EPF among wheat materials were mainly influenced by genotype. However, non-genetic factors were still important, especially for wheat materials with low EPF.展开更多
This study was to find the regularity in the hereditary variation for the main culturing characters of the immature embryo culture in maize. Two kinds of inbred-line, R18-599 （red） with very excellent embryo culturi...This study was to find the regularity in the hereditary variation for the main culturing characters of the immature embryo culture in maize. Two kinds of inbred-line, R18-599 （red） with very excellent embryo culturing capacity and R15 with very poor embryo culturing capacity, were used as P1 and P2 for obtaining six generations. By culturing immature embryos of the six generations, four culturing characters, namely embryonic callus induction efficiency, nonembryonic callus induction efficiency, cloning ability of the embryonic callus, and number of regenerating plants, were analyzed using the general mean analysis and generation joint analysis. Results showed that the embryonic callus induction efficiency accorded with two major additive-dominance-epistatic genes and polygene-mixed additive-dominance-epistatic inheritance model. The induction efficiency of the nonembryonic callus accorded with two major additive-dominance-epistatic genes. The number of regenerating plants accorded with one major gene and polygene-mixed additive-dominance inheritance model. The cloning ability of the embryo callus accorded with two major genes and polygene-mixed inheritance model, whereas the effect of epistatic gene on this character was identified results of the two methods, generation joint analysis may genetic information. to be different using the two methods. By comparison of the not only raise experimental precision but also provide more展开更多
This work presents a performance comparison between several incubators models including CO2 and NH4 emission.A mathematical model for incubators carbon foot print was developed to estimate CO2 and NH4 emission.The pro...This work presents a performance comparison between several incubators models including CO2 and NH4 emission.A mathematical model for incubators carbon foot print was developed to estimate CO2 and NH4 emission.The program written by C++language including convert line.The modular structure of program consists of a main programme and series of independent subroutine:each one deals with a specific parameter of the required data.The computer programme has a wide range of applicability several values of size of the machine(NO.egg),Fertility(F),Heat production embryo(HPe),maximum CO2 level(CO2)m,CO2 level incoming air(CO2)I,RQ value(RQ)to estimate Heat production(HP),CO2 production,Ventilation(V),Ventilation of egg(Vegg)Input data:Enter size of the machine,Fertility(F),Heat production embryo(HPe),maximum CO2 level(CO2)m,CO2 level incoming air(CO2)I,RQ value(RQ)the results As the growth period passed from the first day of the twenty-first day,the amount of heat produced increased from 0.0001 to 0.35 w/egg,and ventilation from 0 to 352 m^3/hr as well as the amount of carbon dioxide produced from 0.0000158 to 0.04318 lit/hr/Mach.As the number of eggs increased from 5,000 to 30,000 eggs,each of the heat produced increased from 923.4 to 5540.4 kg/hr,the resulting carbon dioxide from 32 to 190 lit/hr/Mach,and ventilation from 9 to 54 m^3/hr.展开更多
文摘To regenerate three-dimensional endometrium in vitro as a novel model for studying the mechanism of implantation of embryos, the luminal epithelial cells and stromal cells of the rabbit uterus were separated and cultured in vitro. The type Ⅰ mouse tail collagen was used as scaffolding material. The stromal cells were inoculated in the type I mouse tail collagen, and the luminal epithelial cells were inoculated on the type i mouse tall collagen to regenerate the endometrium in vitro. The regenerated endometrium was cultured in DMEM-F/12 media containing 100 nmol L^-1 progesterone, 10 nM β-estradiol, and 10% fetal bovine serum （FBS） for 3 d. The media were then replaced with CZB containing 100 nM progesterone, 10 nmol L-1 β-estradiol, and 10% FBS, and the mouse blastulas were co-cultured with it. The results of scanning electronic micrography showed that the epithelial cells on the surface of the reconstructed endometrium were covered with numerous slender microvilli and some epithelial cells protruded pinopodes. After culturing for 12 h with the mouse blastula, the shedding, attachment, and implantation of the blastula were observed. The blastula can escape from zona pellucida and attach to the three-dimensional endometrium and is then implanted into it. This study showed that the reconstructed three-dimensional endometrium can serve as a robust embryo implantation model in vitro.
文摘Objective: We established a transplantation tumor model of human osteosarcoma in chick embryo, studied its morphological and biological characteristics, and observed its dynamic process of angiogenesis induction so that a simple and practical model can be provided for studying osteosarcoma. Methods: Human osteosarcoma cells at different concentrations were inoculated in chorioallantoic membrane (CAM) of chick embryos at different embryonic ages to observe the factors affecting the survival of the transplanted osteosarcoma in chick embryo, growth characteristics of the transplantation tumor, and the morphological characteristics and biological characteristics of the transplantation tumor. Results: The transplantation tumor model of human osteosarcoma in chick embryo was successfully established. It was found that the transplantation tumor was easy to grow and it showed strong angiogenesis-inducing effects. Under the light microscope, the transplantation tumor showed a similar tissue structure to human osteosarcoma. Conclusion: It is feasible to establish a transplantation tumor model of human osteosarcoma in chick embryo. The model can be easily duplicated with a simple operation, which provides a useful animal model for studying osteosarcoma.
文摘[Objective] The aim was to study the effect of the content of copper and zinc on in medium the vitamin E accumulation in wheat embryo-dreived callus.[Method] The mathematical models were established to describe the growth kinetics and the vitamin E accumulation in wheat embryo callus cells.With the aim of getting the highest accumulation of the secondary metabolite Vitamin E,the optimal combination of copper and zinc in medium was confirmed by testing.[Result] The results showed that the production of vitamin E in B5 medium reached the highest value with 2.0 mg/mL ZnSO4·7H2O and 0.1 mg/mL CuSO4·5H2O.The fitting degrees of kinetic models of vitamin E accumulation and cell growth were 97.53% and 95.60%,respectively,which indicated good nonlinear relationships.[Conclusion] Both copper and zinc could affect the accumulation of vitamin E in wheat germ callus,and Copper showed more prominent effect than Zn.Synergism existed in low copper and zinc concentration,and the inhibitive effect enhanced with the increase of the concentrations.
基金Supported by National High Technology Research and Development Program of China(863 Program)(2011AA10A106)Yunnan Provincial Fund for Applied Basic Researches(2010CC001)Key New Product Development Plan of Yunnan Province(2012BB015)~~
文摘A DH population derived from C49S-87/01Y1-1069 was used to study the inheritance of wheat haploid embryo production frequency（EPF） in wheat × maize cross with the mixed major gene and polygene inheritance model of quantitative traits. The results showed that the EPF of wheat × maize cross was controlled by two dominant epistatic genes and polygene with gene effects of 1.95 for the first major gene, 6.69 for the second one and 2.80 for the polygene. The inheritability of major genes was as high as 72.09%, suggesting that the differences in EPF among wheat materials were mainly influenced by genotype. However, non-genetic factors were still important, especially for wheat materials with low EPF.
文摘This study was to find the regularity in the hereditary variation for the main culturing characters of the immature embryo culture in maize. Two kinds of inbred-line, R18-599 （red） with very excellent embryo culturing capacity and R15 with very poor embryo culturing capacity, were used as P1 and P2 for obtaining six generations. By culturing immature embryos of the six generations, four culturing characters, namely embryonic callus induction efficiency, nonembryonic callus induction efficiency, cloning ability of the embryonic callus, and number of regenerating plants, were analyzed using the general mean analysis and generation joint analysis. Results showed that the embryonic callus induction efficiency accorded with two major additive-dominance-epistatic genes and polygene-mixed additive-dominance-epistatic inheritance model. The induction efficiency of the nonembryonic callus accorded with two major additive-dominance-epistatic genes. The number of regenerating plants accorded with one major gene and polygene-mixed additive-dominance inheritance model. The cloning ability of the embryo callus accorded with two major genes and polygene-mixed inheritance model, whereas the effect of epistatic gene on this character was identified results of the two methods, generation joint analysis may genetic information. to be different using the two methods. By comparison of the not only raise experimental precision but also provide more
文摘This work presents a performance comparison between several incubators models including CO2 and NH4 emission.A mathematical model for incubators carbon foot print was developed to estimate CO2 and NH4 emission.The program written by C++language including convert line.The modular structure of program consists of a main programme and series of independent subroutine:each one deals with a specific parameter of the required data.The computer programme has a wide range of applicability several values of size of the machine(NO.egg),Fertility(F),Heat production embryo(HPe),maximum CO2 level(CO2)m,CO2 level incoming air(CO2)I,RQ value(RQ)to estimate Heat production(HP),CO2 production,Ventilation(V),Ventilation of egg(Vegg)Input data:Enter size of the machine,Fertility(F),Heat production embryo(HPe),maximum CO2 level(CO2)m,CO2 level incoming air(CO2)I,RQ value(RQ)the results As the growth period passed from the first day of the twenty-first day,the amount of heat produced increased from 0.0001 to 0.35 w/egg,and ventilation from 0 to 352 m^3/hr as well as the amount of carbon dioxide produced from 0.0000158 to 0.04318 lit/hr/Mach.As the number of eggs increased from 5,000 to 30,000 eggs,each of the heat produced increased from 923.4 to 5540.4 kg/hr,the resulting carbon dioxide from 32 to 190 lit/hr/Mach,and ventilation from 9 to 54 m^3/hr.
