Effect of continuous casting speed on mold surface flow and the related near-surface distribution of non-metallic inclusions

For the control of surface defects in interstitial-free(IF) steel, quantitative metallographic analyses of near-surface inclusions and surface liquid flow detection via the nail-board tipping method were conducted. The results show that, at casting speeds of 0.8 and 1.0 m/min, a thin liquid mold flux layer forms and non-uniform floating of argon bubbles occurs, inducing the entrainment and subsequent entrapment of the liquid flux; fine inclusion particles of Al_2O_3 can also aggregate at the solidification front. At higher casting speeds of 1.4 and 1.6 m/min, the liquid mold flux can be entrained and carried deeper into the liquid steel pool because of strong level fluctuations of the liquid steel and the flux. The optimal casting speed is approximately 1.2 m/min, with the most favorable surface flow status and, correspondingly, the lowest number of inclusions near the slab surface.

Current Trends and Perspectives of Detection and Location for Buried Non-Metallic Pipelines

Buried pipelines are an essential component of the urban infrastructure of modern cities.Traditional buried pipes are mainly made of metal materials.With the development of material science and technology in recent years,non-metallic pipes,such as plastic pipes,ceramic pipes,and concrete pipes,are increasingly taking the place of pipes made from metal in various pipeline networks such as water supply,drainage,heat,industry,oil,and gas.The location technologies for the location of the buried metal pipeline have become mature,but detection and location technologies for the non-metallic pipelines are still developing.In this paper,current trends and future perspectives of detection and location of buried non-metallic pipelines are summarized.Initially,this paper reviews and analyzes electromagnetic induction technologies,electromagnetic wave technologies,and other physics-based technologies.It then focuses on acoustic detection and location technologies,and finally introduces emerging technologies.Then the technical characteristics of each detection and location method have been compared,with their strengths and weaknesses identified.The current trends and future perspectives of each buried non-metallic pipeline detection and location technology have also been defined.Finally,some suggestions for the future development of buried non-metallic pipeline detection and location technologies are provided.

Study on non-metallic inclusions in Al killed high strength alloy steel refined by high basicity and high Al_2O_3 content slag

Laboratory and industrial studies were carried out to investigate non-metallic inclusions in high strength alloy steel refined by high basicity and high Al_2O_3 slag.It was found that the steel/slag reaction time largely affected non-metallic inclusions.With the reaction time increased from 30 min to 90 min in laboratory study,MgO-Al_2O_3 spinels were gradually changed into CaO-MgO-Al_2O_3 system inclusions surrounded by softer CaO-Al_2O_3 surface layers.By using high basicity slag which contained as much as 41%Al_2O_3 in the laboratory study,ratio of low melting temperature CaO-MgO-Al_2O_3 system inclusions was remarkably increased to above 80%.In the industrial experiment,during the secondary refining,the inclusions changed in order of 'Al_2O_3→MgO-Al_2O_3→CaO-MgO-Al_2O_3'.Through the LF and RH refining,most inclusions could be transferred to lower melting temperature CaO-Al_2O_3 and CaO-MgO-Al_2O_3 system inclusions.

A presumed iridocyclitis developed to panophthalmitis caused by a non-metallic intraocular foreign body

Dear Editor,I write to present one case report of a patient suffered the panophthalmitis caused by non-metallic foreign body with no etiologic agent and recovered quickly by phacoemulsification and vitrectomy with silicone oil tamponade.Ocular trauma remains a major cause of blindness, particularly in the working-age population.

Three-dimensional analysis technology for accurately characterizing non-metallic inclusion particles in steel

In this paper, a kind of three-dimensional analysis technology for characterizing non-metallic inclusions in steel was clearly elaborated. It is an electron microscopy observation, namely the non-aqueous electrolysis extraction method with a settled coulometer. In the research,the extraction effects of non-metallic inclusions in different electrolysis systems were studied, and it was concluded that alkalescent 2% TEA non-aqueous electrolyte was applicable for extracting most of non-metallic inclusion particles in steel. And then, in order to ensure the microscopic characterization and statistical calculation of inclusion particles, some electrolysis parameters should be confirmed, such as the size of the sample, control of the electrolysis mass, electric current, etc. Furthermore, for preventing the disturbance of carbides and presenting clear three-dimensional appearance by microscopic characterization, magnetic separation was utilized to separate the inclusion particles from carbides, which was useful for getting more veracious types, particle sizes and chemical composition of inclusions. Moreover, through calculation of quantity and particle size of inclusions in continuous determinate fields, the total quantity per unit volume or area and the particle size distribution of inclusions could be acquired by conversion with electrolysis loss. Besides, the comparison between this method and traditional quantitative metallography was also discussed, and finally, a conclusion was drawn that both of them have respectively applications in characterizing inclusions.

Behavior of Non-metallic Inclusions in Centrifugal Induction Electroslag Castings

In order to know the behavior of non-metallic inclusions in centrifugal induction electroslag castings (CIESC), non-metallic inclusions in 5CrMnMo and 4Cr5MoSiV1 were qualitatively and quantitatively analyzed. The largest size of inclusions in the casting and the thermodynamic possibility of TiN precipitation in steel were also calculated. The results show that sulfide inclusions are evenly distributed and the content is low. The amount of oxide inclusions in CIESC: 4Cr5MoSiV1 steel is close to the ESR steel and lower than that in the EAF steel, and there are some differences along radial direction. Nitride inclusions are fine and the diameter of the largest one is 3-1 mum. With the increase of the centrifugal machine's rotational speed, the ratio of round inclusions increases and the ratio of sharp inclusions decreases. According to the experiment and the calculation results, it is pointed out that the largest diameter of non-metallic inclusions in the CIESC 4Cr5MoSiV1 casting is only 6.6 mum, and [N%][Ti%] in 4Cr5MoSiV1 steel should be controlled less than 4.4x 10(-5) in order to further reduce the amount and size of TiN inclusions.

Strategies to achieve effective nitrogen activation

Ammonia serves as a crucial chemical raw material and hydrogen energy carrier.Aqueous electrocatalytic nitrogen reduction reaction(NRR),powered by renewable energy,has attracted tremendous interest during the past few years.Although some achievements have been revealed in aqueous NRR,significant challenges have also been identified.The activity and selectivity are fundamentally limited by nitrogen activation and competitive hydrogen evolution.This review focuses on the hurdles of nitrogen activation and delves into complementary strategies,including materials design and system optimization(reactor,electrolyte,and mediator).Then,it introduces advanced interdisciplinary technologies that have recently emerged for nitrogen activation using high-energy physics such as plasma and triboelectrification.With a better understanding of the corresponding reaction mechanisms in the coming years,these technologies have the potential to be extended in further applications.This review provides further insight into the reaction mechanisms of selectivity and stability of different reaction systems.We then recommend a rigorous and detailed protocol for investigating NRR performance and also highlight several potential research directions in this exciting field,coupling with advanced interdisciplinary applications,in situ/operando characterizations,and theoretical calculations.

Influence of nitrogen status on fermentation performances of non-Saccharomyces yeasts:a review

Nitrogen,one of the most crucial nutrients present in grapes and musts,plays a key role in yeast activities during alcoholic fermentation.Such influences are imposed on yeast growth and fermentation performances including the formation of secondary metabolites.Saccharomyces cerevisiae,the main yeast responsible for fermentation,has been studied extensively regarding nitrogen impacts.On the other hand,a similar study for non-Saccharomyces yeasts,whose contributions to winemaking have gradually been acknowledged,remains to be fully explored,with a few studies being reported.This review starts by discussing nitrogen impacts on non-Saccharomyces yeast growth and fermentation kinetics in different case scenarios,then proceeds to summarize the nitrogen preferences of individual yeast strains with regulation mechanisms elucidated by recent studies.Detailed discussions on the influences on the production of volatile compounds and proposed pathways therein are made,followed by future work suggested as the final section.In summarizing the nitrogen impacts on non-Saccharomyces yeasts throughout alcoholic fermentation,this review will be helpful in obtaining a more comprehensive view on these non-conventional wine yeasts in terms of nutrient requirements and corresponding volatile production.Research gaps will therefore be elucidated for future research.

Increased dependence on nitrogen-fixation of a native legume in competition with an invasive plant

Suppression of roots and/or their symbiotic microorganisms,such as mycorrhizal fungi and rhizobia,is an effective way for alien plants to outcompete native plants.However,little is known about how invasive and native plants interact with the quantity and activity of nutrient-acquisition agents.Here a pot experiment was conducted with monoculture and mixed plantings of an invasive plant,Xanthium strumarium,and a common native legume,Glycine max.We measured traits related to root and nodule quantity and activity and mycorrhizal colonization.Compared to the monoculture,fine root quantity(biomass,surface area)and activity(root nitrogen(N)concentration,acid phosphatase activity)of G.max decreased in mixed plantings;nodule quantity(biomass)decreased by 45%,while nodule activity in Nfixing via rhizobium increased by 106%;mycorrhizal colonization was unaffected.Contribution of N fixation to leaf N content in G.max increased in the mixed plantings,and this increase was attributed to a decrease in the rhizosphere soil N of G.max in the mixed plantings.Increased root quantity and activity,along with a higher mycorrhizal association was observed in X.strumarium in the mixed compared to monoculture.Together,the invasive plant did not directly scavenge N from nodule-fixed N,but rather depleted the rhizosphere soil N of the legume,thereby stimulating the activity of N-fixation and increasing the dependence of the native legume on this N source.The quantity-activity framework holds promise for future studies on how native legumes respond to alien plant invasions.

Non-metallic Inclusions in Continuously Cast Aluminum Killed Steels

In aluminum killed steels, the size, shape, quantity and formation of non-metallic inclusions in ladle steel (before and after RH vacuum treatment) and in tundish as well as in slabs were studied by EPMA (Electron Probe Microanalysis) and by analyzing the total oxygen. The results showed that in the slabs the total oxygen was quite low and the inclusions discovered were mainly small-sized angular alumina inclusions. This indicates that most inclusions have been removed by floating out during the continuous casting process. In addition, the countermeasures were discussed to decrease the alumina inclusions in the slabs further.

ZmbZIP27 regulates nitrogen-mediated leaf angle by modulating lignin deposition in maize

In grain crops such as maize(Zea mays),leaf angle(LA)is a key agronomic trait affecting light interception and thus planting density and yield.Nitrogen(N)affects LA in plants,but we lack a good understanding of how N regulates LA.Here,we report that N deficiency enhanced lignin deposition in the ligular region of maize seedlings.In situ hybridization showed that the bZIP transcription factor gene ZmbZIP27 is mainly expressed in the phloem of maize vascular bundles.Under N-sufficient conditions,transgenic maize overexpressing ZmbZIP27 showed significantly smaller LA compared with wild type(WT).By contrast,zmbzip27_(ems)mutant showed larger LA under both N-deficient and N-sufficient conditions compared with WT.Overexpression of ZmbZIP27 enhanced lignin deposition in the ligular region of maize in the field.We further demonstrated that ZmbZIP27 could directly bind the promoters of the microRNA genes ZmMIR528a and ZmMIR528b and negatively regulate the expression levels of ZmmiR528.ZmmiR528 knockdown transgenic maize displayed erect architecture in the field by increasing lignin content in the ligular region of maize.Taken together,these results indicate that ZmbZIP27 regulates N-mediated LA size by regulating the expression of ZmmiR528 and modulating lignin deposition in maize.

Effects of the kinetic pattern of dietary glucose release on nitrogen utilization, the portal amino acid profile, and nutrient transporter expression in intestinal enterocytes in piglets

Background Promoting the synchronization of glucose and amino acid release in the digestive tract of pigs could effectively improve dietary nitrogen utilization.The rational allocation of dietary starch sources and the exploration of appropriate dietary glucose release kinetics may promote the dynamic balance of dietary glucose and amino acid supplies.However,research on the effects of diets with different glucose release kinetic profiles on amino acid absorption and portal amino acid appearance in piglets is limited.This study aimed to investigate the effects of the kinetic pattern of dietary glucose release on nitrogen utilization,the portal amino acid profile,and nutrient transporter expression in intestinal enterocytes in piglets.Methods Sixty-four barrows(15.00±1.12 kg)were randomly allotted to 4 groups and fed diets formulated with starch from corn,corn/barley,corn/sorghum,or corn/cassava combinations(diets were coded A,B,C,or D respectively).Protein retention,the concentrations of portal amino acid and glucose,and the relative expression of amino acid and glucose transporter m RNAs were investigated.In vitro digestion was used to compare the dietary glucose release profiles.Results Four piglet diets with different glucose release kinetics were constructed by adjusting starch sources.The in vivo appearance dynamics of portal glucose were consistent with those of in vitro dietary glucose release kinetics.Total nitrogen excretion was reduced in the piglets in group B,while apparent nitrogen digestibility and nitrogen retention increased(P<0.05).Regardless of the time(2 h or 4 h after morning feeding),the portal total free amino acids content and contents of some individual amino acids(Thr,Glu,Gly,Ala,and Ile)of the piglets in group B were significantly higher than those in groups A,C,and D(P<0.05).Cluster analysis showed that different glucose release kinetic patterns resulted in different portal amino acid patterns in piglets,which decreased gradually with the extension of feeding time.The portal His/Phe,Pro/Glu,Leu/Val,Lys/Met,Tyr/Ile and Ala/Gly appeared higher similarity among the diet treatments.In the anterior jejunum,the glucose transporter SGLT1 was significantly positively correlated with the amino acid transporters B0AT1,EAAC1,and CAT1.Conclusions Rational allocation of starch resources could regulate dietary glucose release kinetics.In the present study,group B(corn/barley)diet exhibited a better glucose release kinetic pattern than the other groups,which could affect the portal amino acid contents and patterns by regulating the expression of amino acid transporters in the small intestine,thereby promoting nitrogen deposition in the body,and improving the utilization efficiency of dietary nitrogen.

Microfluidic-oriented synthesis of enriched iridium nanodots/carbon architecture for robust electrocatalytic nitrogen fixation

Electrocatalytic nitrogen reduction reaction(NRR)is considered as a promising candidate to achieve ammonia synthesis because of clean electric energy,moderate reaction condition,safe operating process and harmless by-products.However,the chemical inertness of nitrogen and poor activated capacity on catalyst surface usually produce low ammonia yield and faradic efficiency.Herein,the microfluidic technology is proposed to efficiently fabricate enriched iridium nanodots/carbon architecture.Owing to in-situ co-precipitation reaction and microfluidic manipulation,the iridium nanodots/carbon nanomaterials possess small average size,uniform dispersion,high conductivity and abundant active sites,producing good proton activation and rapid electrons transmission and moderate adsorption/desorption capacity.As a result,the as-prepared iridium nanodots/carbon nanomaterials realize large ammonia yield of 28.73 μg h^(-1) cm^(-2) and faradic efficiency of 9.14%in KOH solution.Moreover,the high ammonia yield of 11.21 μg h^(-1) cm^(-2) and faradic efficiency of 24.30%are also achieved in H_(2)SO_(4) solution.The microfluidic method provides a reference for large-scale fabrication of nano-sized catalyst materials,which may accelerate the progress of electrocatalytic NRR in industrialization field.

Evaluation of Characteristics of Non-Metallic Inclusions in P/M Ni-Base Superalloy by Automatic Image Analysis

Non-metallic inclusions,especially the large ones,within P/M Ni-base superalloy have a major influence on fatigue characteristics,but are not directly measurable by routine inspection.In this paper,a method,automatic image analysis,is proposed for estimation of the content,size and amount of non-metallic inclusions in superalloy.The methodology for the practical application of this method is described and the factors affecting the precision of the estimation are discussed.In the experiment,the characteristics of the non-metallic inclusions in Ni-base P/M superalloy are analyzed.

Regulation of 2-acetyl-1-pyrroline and grain quality in early-season indica fragrant rice by nitrogen and silicon fertilization under different plantation methods

Fragrant rice has a high market value,and it is a popular rice type among consumers owing to its pleasant flavor.Plantation methods,nitrogen(N)fertilizers,and silicon(Si)fertilizers can affect the grain yield and fragrance of fragrant rice.However,the core commercial rice production attributes,namely the head rice yield(HRY)and 2-acetyl-1-pyrroline(2-AP)content of fragrant rice,under various nitrogen and silicon(N-Si)fertilization levels and different plantation methods remain unknown.The field experiment in this study was performed in the early seasons of 2018 and 2019 with two popular indica fragrant rice cultivars(Yuxiangyouzhan and Xiangyaxiangzhan).They were grown under six N-Si fertilization treatments(combinations of two levels of Si fertilizer,0 kg Si ha^(−1)(Si0)and 150 kg Si ha^(−1)(Si1),and three levels of N fertilizer,0 kg N ha^(−1)(N0),150 kg N ha^(−1)(N1),and 220 kg N ha^(−1)(N2))and three plantation methods(artificial transplanting(AT),mechanical transplanting(MT),and mechanical direct-seeding(MD)).The results showed that the N-Si fertilization treatments and all the plantation methods significantly affected the HRY and 2-AP content and related parameters of the two different fragrant rice cultivars.Compared with the Si0N0 treatment,the N-Si fertilization treatments resulted in higher HRY and 2-AP contents.The rates of brown rice,milled rice,head rice,and chalky rice of the fragrant rice also improved with the N-Si fertilization treatments.The N-Si fertilization treatments increased the activities of N metabolism enzymes and the accumulation of N and Si in various parts of the fragrant rice,and affected their antioxidant response parameters.The key parameters for the HRY and 2-AP content were assessed by redundancy analysis.Furthermore,the structural equation model revealed that the Si and N accumulation levels indirectly affected the HRY by affecting the N metabolism enzyme activity,N use efficiency,and grain quality of fragrant rice.Moreover,high N and Si accumulation directly promoted the 2-AP content or affected the antioxidant response parameters and indirectly regulated 2-AP synthesis.The interactions of the MT method with the N-Si fertilization treatments varied in the fragrant rice cultivars in terms of the HRY and 2-AP content,whereas the MD method was beneficial to the 2-AP content in both fragrant rice cultivars under the N-Si fertilization treatments.

Composition Engineering Opens an Avenue Toward Efficient and Sustainable Nitrogen Fixation

In this work,we open an avenue toward rational design of potential efficient catalysts for sustainable ammonia synthesis through composition engineering strategy by exploiting the synergistic effects among the active sites as exemplified by diatomic metals anchored graphdiyne via the combination of hierarchical high-throughput screening,first-principles calculations,and molecular dynamics simulations.Totally 43 highly efficient catalysts feature ultralow onset potentials(|U_(onset)|≤0.40 V)with Rh-Hf and Rh-Ta showing negligible onset potentials of 0 and-0.04 V,respectively.Extremely high catalytic activities of Rh-Hf and Rh-Ta can be ascribed to the synergistic effects.When forming heteronuclears,the combinations of relatively weak(such as Rh)and relatively strong(such as Hf or Ta)components usually lead to the optimal strengths of adsorption Gibbs free energies of reaction intermediates.The origin can be ascribed to the mediate d-band centers of Rh-Hf and Rh-Ta,which lead to the optimal adsorption strengths of intermediates,thereby bringing the high catalytic activities.Our work provides a new and general strategy toward the architecture of highly efficient catalysts not only for electrocatalytic nitrogen reduction reaction(eNRR)but also for other important reactions.We expect that our work will boost both experimental and theoretical efforts in this direction.

The extending length calculation of hydraulic drive non-metallic completion screen pipe running into ultra-short radius horizontal well

Focusing on the extending length restriction of the completion screen pipe resistance running into ultra-short radius horizontal well,this paper proposed technology of hydraulic drive completion tubular string running into ultra-short radius horizontal well.Innovative hydraulic drive tools and string structure are designed,which are composed of guide tubing,hydraulic drive tubing and non-metallic completion screen pipe from inside to outside.A novel mechanical-hydraulic coupling model is established.Based on the wellbore structure of an ultra-short radius horizontal well for deep coalbed methane,the numerical calculations of force and hydraulic load on tubular strings were accomplished by the mechanical-hydraulic coupling model.The results show that the extending length of completion tubular string with the hydraulic drive is 17 times that of conventional completion technology under the same conditions.The multi-factor orthogonal design is adopted to analyze the numerical calculations,and the results show that the extending length of the completion tubular string is mainly affected by the completion tubular string structure and the friction coefficient between the non-metallic composite continuous screen pipe and the wellbore.Two series of hydraulic drive completion tubular string structures suitable for ultra-short radius horizontal wells under different conditions are optimized,with the extending limits of 381 m and 655 m,respectively.These researches will provide theoretical guidance for design and control of hydraulic drive non-metallic composite continuous completion screen pipe running into ultra-short radius horizontal wells.

Identification of the lysine and histidine transporter family in Camellia sinensis and the characterizations in nitrogen utilization

In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino acids.The tea plant(Camellia sinensis)is a perennial evergreen with a relatively high level of amino acids.However,systematic identification and molecular characterization of the LHT gene family has rarely been reported in tea plants.In this study,22 CsLHTs were identified from the‘Shuchazao’genome and classified into two groups.The modeled three-dimensional structure and the conserved domains presented a high similarity among the LHTs proteins.Moreover,it was predicted that a few genes were conserved through the analysis of the physiochemical characters,structures and cis-elements in promoters.The expression patterns in tea plants revealed that CsLHT7 was mainly expressed in the roots,and CsLHT4 and CsLHT11 exhibited relatively high expression in both the roots and leaves.Moreover,the expression of all three genes could be induced by organic nitrogen.Additionally,heterogeneous expression of CsLHT4,CsLHT7 and CsLHT11 in Arabidopsis thaliana decreased the aerial parts biomass compared with that in WT plants while significantly increased the rosette biomass only for CsLHT11transgenic plants versus WT plants.Overall,our results provide fundamental information about CsLHTs and potential genes in N utilization for further analysis in tea plants.

Studies of non-metallic organic disinfectants on inactivation of avian influenza viruses

Six different kinds of non-metallic or organic disinfectants were obtained in this research study including “Neutral Electrolyzed Water”, “M22” organic disinfectant solution, Superoxy Food Wash disinfectant, Hydrogen Peroxide, Clorox Germicidal Bleach and Clidox-S. The effectiveness of these disinfectants was studied against various subtypes of avian influenza virus (AIV). The virus-disinfectant mixtures were prepared in serial dilutions of each disinfectant with a constant virus titer and incubated at ambient temperature in different time intervals for virus inactivation. The virus inactivation results were determined by virus recovery in embryonating chicken eggs. Among the six different kinds of nonmetallic disinfectants obtained for this research project, Neutral Electrolyzed Water, “M22” solution, Clorox Germicidal Bleach and Clidox-S were effectively inactivated AIV with appropriate working dilutions and reaction times. Superoxy Food Wash disinfectant and Hydrogen Peroxide were found having limited effect on virus inactivation with extended exposure times of more than 2 hours. These research findings provide scientific data to poultry industry with guidelines to select and use non-metallic organic disinfectants for poultry flock sanitation and disinfection to effectively prevent and control of avian influenza outbreaks.

Nitrogen rhizodeposition from corn and soybean,and its contribution to the subsequent wheat crops

Nitrogen(N)is a key factor in the positive response of cereal crops that follow leguminous crops when compared to gramineous crops in rotations,with the nonrecyclable rhizosphere-derived N playing an important role.However,quantitative assessments of differences in the N derived from rhizodeposition(NdfR)between legumes and gramineous crops are lacking,and comparative studies on their contributions to the subsequent cereals are scarce.In this study,we conducted a meta-analysis of NdfR from leguminous and gramineous crops based on 34 observations published worldwide.In addition,pot experiments were conducted to study the differences in the NdfR amounts,distributions and subsequent effects of two major wheat(Triticum aestivum L.)-preceding crops,corn(Zea mays L.)and soybean(Glycine max L.),by the cotton wick-labelling method in the main wheat-producing areas of China.The meta-analysis results showed that the NdfR of legumes was significantly greater by 138.93%compared to gramineous crops.In our pot experiment,the NdfR values from corn and soybean were 502.32 and 944.12 mg/pot,respectively,and soybean was also significantly higher than corn,accounting for 76.91 and 84.15%of the total belowground nitrogen of the plants,respectively.Moreover,in different soil particle sizes,NdfR was mainly enriched in the large macro-aggregates(>2 mm),followed by the small macro-aggregates(2–0.25 mm).The amount and proportion of NdfR in the macro-aggregates(>0.25 mm)of soybean were 3.48 and 1.66 times higher than those of corn,respectively,indicating the high utilization potential of soybean NdfR.Regarding the N accumulation of subsequent wheat,the contribution of soybean NdfR to wheat was approximately 3 times that of corn,accounting for 8.37 and 4.04%of the total N uptake of wheat,respectively.In conclusion,soybean NdfR is superior to corn in terms of the quantity and distribution ratio of soil macro-aggregates.In future field production,legume NdfR should be included in the nitrogen pool that can be absorbed and utilized by subsequent crops,and the role and potential of leguminous plants as nitrogen source providers in crop rotation systems should be fully utilized.