The damage evolution process of non-penetrating cracks often causes some unexpected engineering disasters.Gypsum specimens containing non-penetrating crack(s)are used to study the damage evolution and characteristics ...The damage evolution process of non-penetrating cracks often causes some unexpected engineering disasters.Gypsum specimens containing non-penetrating crack(s)are used to study the damage evolution and characteristics under cyclic loading.The results show that under cyclic loading,the relationship between the number of non-penetrating crack(s)and the characteristic parameters(cyclic number,peak stress,peak strain,failure stress,and failure strain)of the pre-cracked specimens can be represented by a decreasing linear function.The damage evolution equation is fitted by calibrating the accumulative plastic strain for each cycle,and the damage constitutive equation is proposed by the concept of effective stress.Additionally,non-penetrating cracks are more likely to cause uneven stress distribution,damage accumulation,and local failure of specimen.The local failure can change the stress distribution and relieve the inhibition of non-penetrating crack extension and eventually cause a dramatic destruction of the specimen.Therefore,the evolution process caused by non-penetrating cracks can be regarded as one of the important reasons for inducing rockburst.These results are expected to improve the understanding of the process of spalling formation and rockburst and can be used to analyze the stability of rocks or rock structures.展开更多
Quantifying surface cracks in alpine meadows is a prerequisite and a key aspect in the study of grassland crack development.Crack characterization indices are crucial for the quantitative characterization of complex c...Quantifying surface cracks in alpine meadows is a prerequisite and a key aspect in the study of grassland crack development.Crack characterization indices are crucial for the quantitative characterization of complex cracks,serving as vital factors in assessing the degree of cracking and the development morphology.So far,research on evaluating the degree of grassland degradation through crack characterization indices is rare,especially the quantitative analysis of the development of surface cracks in alpine meadows is relatively scarce.Therefore,based on the phenomenon of surface cracking during the degradation of alpine meadows in some regions of the Qinghai-Tibet Plateau,we selected the alpine meadow in the Huangcheng Mongolian Township,Menyuan Hui Autonomous County,Qinghai Province,China as the study area,used unmanned aerial vehicle(UAV)sensing technology to acquire low-altitude images of alpine meadow surface cracks at different degrees of degradation(light,medium,and heavy degradation),and analyzed the representative metrics characterizing the degree of crack development by interpreting the crack length,length density,branch angle,and burrow(rat hole)distribution density and combining them with in situ crack width and depth measurements.Finally,the correlations between the crack characterization indices and the soil and root parameters of sample plots at different degrees of degradation in the study area were analyzed using the grey relation analysis.The results revealed that with the increase of degradation,the physical and chemical properties of soil and the mechanical properties of root-soil composite changed significantly,the vegetation coverage reduced,and the root system aggregated in the surface layer of alpine meadow.As the degree of degradation increased,the fracture morphology developed from"linear"to"dendritic",and eventually to a complex and irregular"polygonal"pattern.The crack length,width,depth,and length density were identified as the crack characterization indices via analysis of variance.The results of grey relation analysis also revealed that the crack length,width,depth,and length density were all highly correlated with root length density,and as the degradation of alpine meadows intensified,the underground biomass increased dramatically,forming a dense layer of grass felt,which has a significant impact on the formation and expansion of cracks.展开更多
Soybean is an important upland crop,but its productivity is often limited by anaerobic stress caused by waterlogging.The ability to adjust root growth under environmental constraints is an important physiological trai...Soybean is an important upland crop,but its productivity is often limited by anaerobic stress caused by waterlogging.The ability to adjust root growth under environmental constraints is an important physiological trait for adapting to an everchanging environment,and root pruning is an artificial technique for regenerating the root system.In the present study,we investigated whether root pruning in soybean can effectively alleviate the inhibitory effects of anaerobic stress.Soybean plants were affected by anaerobic stress at the germination,vegetative stage 1(V1),and reproductive stage 1(R1)stages,and then the plants were treated with root pruning just after the stress treatment.Soybean plants at the germination stage were treated with root cap and tip removals after hypoxia(N_(2)treatment).Root cap removal was more effective in suppressing the inhibitory effects of hypoxia than root tip removal(5 mm from the tip).The shoot dry weights of the soybean plants with and without root cap removal after hypoxia were 51.2 and 73.8%of the control,respectively,while the root dry weights of plants with and without root cap removal after hypoxia were 43.2 and 62.8%of the control,respectively.As root cap removal effectively enhanced soybean growth after anaerobic stress,the root cap may be the candidate tissue for the stress memory mechanism.When soybean plants at the V1 stage of growth were affected by anaerobic stress,the branch number,pod weight in the main stem,root length,and root surface area of the soybean plants treated with anaerobic stress at the R1 stage significantly decreased compared with those of the control.In contrast,root pruning(2 mm from the tip)immediately after the stress treatment enhanced root growth,branch number,and pod weight.The branch number,pod weight,root length,and root surface area of the plants treated with root pruning were 1.13,1.14,1.12,and 1.13 times higher than those of plants treated with anaerobic stress.Plasmolysis was observed in the root meristem,columella,and cortical cells in soybean roots subjected to anaerobic conditions.However,damage was not observed in the newly emerged roots after root pruning in plants treated with anaerobic stress.These results suggested that root pruning is effective in enhancing soybean growth after anaerobic stress.This effectiveness may be due to the regeneration and elongation of healthy lateral roots during the recovery period.When soybean plants were affected by anaerobic stress at the R1 stage,root pruning just after the stress treatment was ineffective.Thus,suppressing the growth reduction due to anaerobic stress at reproductive stages using only root pruning may be difficult.展开更多
目的研究根管预备、充填及纤维桩预备对根管壁微裂产生的影响。方法收集150颗离体前磨牙,并随机分为四个大组[1组(n=10)、2组(n=20)、3组(n=40)、4组(n=80)]及15个小组(1组,2组25#、2组40#,3组A25#、3组B25#、3组A40#、3组B40#,4组AX25#...目的研究根管预备、充填及纤维桩预备对根管壁微裂产生的影响。方法收集150颗离体前磨牙,并随机分为四个大组[1组(n=10)、2组(n=20)、3组(n=40)、4组(n=80)]及15个小组(1组,2组25#、2组40#,3组A25#、3组B25#、3组A40#、3组B40#,4组AX25#、4组AX40#、4组AT25#、4组AT40#、4组BX25#、4组BX40#、4组BT25#、4组BT40#,n=10)。1组:即对照组,不进行预备;2组:均分为两组,一组预备至WaveOne25#,另一组预备则预备至40#;3组:将样本与2组一样进行分组预备,之后进一步分为2个小组,进行冷牙胶侧压充填(A)及热牙胶连续波充填(B);4组:将样本与3组一样进行分组预备及充填,之后将不同方法充填后的样本进一步分为2小组进行RelyXTM(X)和RTD(T)纤维桩预备。将所有的样本从距离根尖3、5、7 mm处垂直于牙体长轴截断,在显微镜下观察微裂发生情况,并进行比较分析。结果1组及2组中均并未有裂纹存在。充填后,3组A与3组B的裂纹数目比较差异无统计学意义(P>0.05)。置入纤维桩后,4组A的牙本质裂纹明显较4组B多,且差异具有统计学意义(22/120 VS 10/120,P<0.05)。在与根尖距离7 mm处,相较于RelyXTM纤维桩,RTD纤维桩预备后牙本质产生更多裂纹,差异具有统计学意义(9/120 VS 23/120,P<0.05)。4组的裂纹总发生率高于1组、2组、3组(P<0.05),说明纤维桩置入后,牙本质裂纹较仅进行牙体预备和充填明显增多。结论纤维桩的预备主要导致根管壁裂纹的产生,冷侧压充填后进行纤维桩预备比热牙胶充填后进行桩体预备更容易产生根管壁裂纹。展开更多
Cruciform joints in ships are prone to fatigue damage and the determination of type of weld plays a significant role in thefatigue design of the joint. In this paper, the effect of weld geometry on fatigue failure of ...Cruciform joints in ships are prone to fatigue damage and the determination of type of weld plays a significant role in thefatigue design of the joint. In this paper, the effect of weld geometry on fatigue failure of load carrying cruciform joints inships is investigated using Effective Notch Stress (ENS) approach. A fictitious notch of 1 mm radius is introduced at theweld root and toe and fatigue stress is evaluated. The effect of weld leg length (l) and weld penetration depth (p) on ENS atweld root and toe are determined. The critical weld leg length (lcr) at which fatigue failure transitions from weld root toweld toe is investigated. An approximation formula for determination of the critical weld leg length considering weldpenetration depth (p) is proposed.展开更多
Peanut root invasion by Bradyrhizobia is through a crack entry, which is different from many other legumes applying an infection thread entry in root hair. Understanding the role of root hair in the crack entry of Bra...Peanut root invasion by Bradyrhizobia is through a crack entry, which is different from many other legumes applying an infection thread entry in root hair. Understanding the role of root hair in the crack entry of Bradyrhizobia invasion of peanut root and subsequent peanut nodulation would facilitate improvement of biological nitrogen fixation in cultivated peanut. The objective of this study was to investigate the involvement of root hair in Bradyrhizobial invasion of peanut. Seedling roots of a nodulating peanut cultivar were observed for root hair emergence, its life span, and nodule formation at the base of the lateral roots with and without rhizobia inoculation for 14 days after germination (DAG). Scanning electron microscopy (SEM) was utilized to observe rhizobia accumulation at lateral roots at 24 hours after inoculation (HAI) before the emergence of root hair. Root hair emerged at 7 DAG with or without rhizobia inoculation. Two variations of rosette hair (RoH) were observed, the transient-thin RoH had life span of 3 days after root hair emergence and the thick and densely distributed RoH type stayed till the time of nodule emergence (9 days after inoculation). The lateral root devoid of root hair at the top 2 cm region was found to produce nodules. The SEM observation of seedling roots at 24 HAI showed that Bradyrhizobia invaded the roots at epidermis, protoplasm of cortical cell, and cortical cells of the main root near the newly emerged lateral root in the absence of RoH. The observations validated that root hair is not required in the Bradyrhizobia invasion of peanut root in the crack entry mode. Results from this study provided important morphological information for the hypothesis of close relationship between RoH and peanut nodulation for further genetic study of crack entry mechanism and signaling pathway of symbiosis between Bradyrhizobia and peanut.展开更多
基金supported by the National Natural Science Foundation of China(Nos.52204092 and 52274203).
文摘The damage evolution process of non-penetrating cracks often causes some unexpected engineering disasters.Gypsum specimens containing non-penetrating crack(s)are used to study the damage evolution and characteristics under cyclic loading.The results show that under cyclic loading,the relationship between the number of non-penetrating crack(s)and the characteristic parameters(cyclic number,peak stress,peak strain,failure stress,and failure strain)of the pre-cracked specimens can be represented by a decreasing linear function.The damage evolution equation is fitted by calibrating the accumulative plastic strain for each cycle,and the damage constitutive equation is proposed by the concept of effective stress.Additionally,non-penetrating cracks are more likely to cause uneven stress distribution,damage accumulation,and local failure of specimen.The local failure can change the stress distribution and relieve the inhibition of non-penetrating crack extension and eventually cause a dramatic destruction of the specimen.Therefore,the evolution process caused by non-penetrating cracks can be regarded as one of the important reasons for inducing rockburst.These results are expected to improve the understanding of the process of spalling formation and rockburst and can be used to analyze the stability of rocks or rock structures.
基金This study was funded by the National Natural Science Foundation of China(42062019,42002283)the Project of Qinghai Science&Technology Department(2021-ZJ-927).
文摘Quantifying surface cracks in alpine meadows is a prerequisite and a key aspect in the study of grassland crack development.Crack characterization indices are crucial for the quantitative characterization of complex cracks,serving as vital factors in assessing the degree of cracking and the development morphology.So far,research on evaluating the degree of grassland degradation through crack characterization indices is rare,especially the quantitative analysis of the development of surface cracks in alpine meadows is relatively scarce.Therefore,based on the phenomenon of surface cracking during the degradation of alpine meadows in some regions of the Qinghai-Tibet Plateau,we selected the alpine meadow in the Huangcheng Mongolian Township,Menyuan Hui Autonomous County,Qinghai Province,China as the study area,used unmanned aerial vehicle(UAV)sensing technology to acquire low-altitude images of alpine meadow surface cracks at different degrees of degradation(light,medium,and heavy degradation),and analyzed the representative metrics characterizing the degree of crack development by interpreting the crack length,length density,branch angle,and burrow(rat hole)distribution density and combining them with in situ crack width and depth measurements.Finally,the correlations between the crack characterization indices and the soil and root parameters of sample plots at different degrees of degradation in the study area were analyzed using the grey relation analysis.The results revealed that with the increase of degradation,the physical and chemical properties of soil and the mechanical properties of root-soil composite changed significantly,the vegetation coverage reduced,and the root system aggregated in the surface layer of alpine meadow.As the degree of degradation increased,the fracture morphology developed from"linear"to"dendritic",and eventually to a complex and irregular"polygonal"pattern.The crack length,width,depth,and length density were identified as the crack characterization indices via analysis of variance.The results of grey relation analysis also revealed that the crack length,width,depth,and length density were all highly correlated with root length density,and as the degradation of alpine meadows intensified,the underground biomass increased dramatically,forming a dense layer of grass felt,which has a significant impact on the formation and expansion of cracks.
基金supported by the Japan Society for the Promotion of Science(JSPS)KAKENHI(JP20K06003 to M.I.)。
文摘Soybean is an important upland crop,but its productivity is often limited by anaerobic stress caused by waterlogging.The ability to adjust root growth under environmental constraints is an important physiological trait for adapting to an everchanging environment,and root pruning is an artificial technique for regenerating the root system.In the present study,we investigated whether root pruning in soybean can effectively alleviate the inhibitory effects of anaerobic stress.Soybean plants were affected by anaerobic stress at the germination,vegetative stage 1(V1),and reproductive stage 1(R1)stages,and then the plants were treated with root pruning just after the stress treatment.Soybean plants at the germination stage were treated with root cap and tip removals after hypoxia(N_(2)treatment).Root cap removal was more effective in suppressing the inhibitory effects of hypoxia than root tip removal(5 mm from the tip).The shoot dry weights of the soybean plants with and without root cap removal after hypoxia were 51.2 and 73.8%of the control,respectively,while the root dry weights of plants with and without root cap removal after hypoxia were 43.2 and 62.8%of the control,respectively.As root cap removal effectively enhanced soybean growth after anaerobic stress,the root cap may be the candidate tissue for the stress memory mechanism.When soybean plants at the V1 stage of growth were affected by anaerobic stress,the branch number,pod weight in the main stem,root length,and root surface area of the soybean plants treated with anaerobic stress at the R1 stage significantly decreased compared with those of the control.In contrast,root pruning(2 mm from the tip)immediately after the stress treatment enhanced root growth,branch number,and pod weight.The branch number,pod weight,root length,and root surface area of the plants treated with root pruning were 1.13,1.14,1.12,and 1.13 times higher than those of plants treated with anaerobic stress.Plasmolysis was observed in the root meristem,columella,and cortical cells in soybean roots subjected to anaerobic conditions.However,damage was not observed in the newly emerged roots after root pruning in plants treated with anaerobic stress.These results suggested that root pruning is effective in enhancing soybean growth after anaerobic stress.This effectiveness may be due to the regeneration and elongation of healthy lateral roots during the recovery period.When soybean plants were affected by anaerobic stress at the R1 stage,root pruning just after the stress treatment was ineffective.Thus,suppressing the growth reduction due to anaerobic stress at reproductive stages using only root pruning may be difficult.
文摘目的研究根管预备、充填及纤维桩预备对根管壁微裂产生的影响。方法收集150颗离体前磨牙,并随机分为四个大组[1组(n=10)、2组(n=20)、3组(n=40)、4组(n=80)]及15个小组(1组,2组25#、2组40#,3组A25#、3组B25#、3组A40#、3组B40#,4组AX25#、4组AX40#、4组AT25#、4组AT40#、4组BX25#、4组BX40#、4组BT25#、4组BT40#,n=10)。1组:即对照组,不进行预备;2组:均分为两组,一组预备至WaveOne25#,另一组预备则预备至40#;3组:将样本与2组一样进行分组预备,之后进一步分为2个小组,进行冷牙胶侧压充填(A)及热牙胶连续波充填(B);4组:将样本与3组一样进行分组预备及充填,之后将不同方法充填后的样本进一步分为2小组进行RelyXTM(X)和RTD(T)纤维桩预备。将所有的样本从距离根尖3、5、7 mm处垂直于牙体长轴截断,在显微镜下观察微裂发生情况,并进行比较分析。结果1组及2组中均并未有裂纹存在。充填后,3组A与3组B的裂纹数目比较差异无统计学意义(P>0.05)。置入纤维桩后,4组A的牙本质裂纹明显较4组B多,且差异具有统计学意义(22/120 VS 10/120,P<0.05)。在与根尖距离7 mm处,相较于RelyXTM纤维桩,RTD纤维桩预备后牙本质产生更多裂纹,差异具有统计学意义(9/120 VS 23/120,P<0.05)。4组的裂纹总发生率高于1组、2组、3组(P<0.05),说明纤维桩置入后,牙本质裂纹较仅进行牙体预备和充填明显增多。结论纤维桩的预备主要导致根管壁裂纹的产生,冷侧压充填后进行纤维桩预备比热牙胶充填后进行桩体预备更容易产生根管壁裂纹。
文摘Cruciform joints in ships are prone to fatigue damage and the determination of type of weld plays a significant role in thefatigue design of the joint. In this paper, the effect of weld geometry on fatigue failure of load carrying cruciform joints inships is investigated using Effective Notch Stress (ENS) approach. A fictitious notch of 1 mm radius is introduced at theweld root and toe and fatigue stress is evaluated. The effect of weld leg length (l) and weld penetration depth (p) on ENS atweld root and toe are determined. The critical weld leg length (lcr) at which fatigue failure transitions from weld root toweld toe is investigated. An approximation formula for determination of the critical weld leg length considering weldpenetration depth (p) is proposed.
文摘Peanut root invasion by Bradyrhizobia is through a crack entry, which is different from many other legumes applying an infection thread entry in root hair. Understanding the role of root hair in the crack entry of Bradyrhizobia invasion of peanut root and subsequent peanut nodulation would facilitate improvement of biological nitrogen fixation in cultivated peanut. The objective of this study was to investigate the involvement of root hair in Bradyrhizobial invasion of peanut. Seedling roots of a nodulating peanut cultivar were observed for root hair emergence, its life span, and nodule formation at the base of the lateral roots with and without rhizobia inoculation for 14 days after germination (DAG). Scanning electron microscopy (SEM) was utilized to observe rhizobia accumulation at lateral roots at 24 hours after inoculation (HAI) before the emergence of root hair. Root hair emerged at 7 DAG with or without rhizobia inoculation. Two variations of rosette hair (RoH) were observed, the transient-thin RoH had life span of 3 days after root hair emergence and the thick and densely distributed RoH type stayed till the time of nodule emergence (9 days after inoculation). The lateral root devoid of root hair at the top 2 cm region was found to produce nodules. The SEM observation of seedling roots at 24 HAI showed that Bradyrhizobia invaded the roots at epidermis, protoplasm of cortical cell, and cortical cells of the main root near the newly emerged lateral root in the absence of RoH. The observations validated that root hair is not required in the Bradyrhizobia invasion of peanut root in the crack entry mode. Results from this study provided important morphological information for the hypothesis of close relationship between RoH and peanut nodulation for further genetic study of crack entry mechanism and signaling pathway of symbiosis between Bradyrhizobia and peanut.